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The map label for this gene is btuB [H]

Identifier: 86751779

GI number: 86751779

Start: 5295193

End: 5297307

Strand: Direct

Name: btuB [H]

Synonym: RPB_4681

Alternate gene names: 86751779

Gene position: 5295193-5297307 (Clockwise)

Preceding gene: 86751775

Following gene: 86751780

Centisome position: 99.32

GC content: 64.87

Gene sequence:

>2115_bases
ATGCCGTCTCGTTCCGATGCCGATTGCCGGCATCATTCCGCGCGCTGCCGCGCGCTGCTGACATCCACCATTCTGGCGTC
CAGTCTGTTCGCGAGCGTCGCGCCGGCGTCGGCGCAGGACAATACGCTGCCCGCCATCAACGTCACCGCGACCCGCAGCT
ACGAGGGCATCGTCGGCACTTCGAGCACGGTGATCACCGCCGACGACATCGCGCATTCGCCGGCGCAGACCGTGCAGGAG
ATCATCGCGCAGACGCCCGGCGTGCAGACCAGGACGCTGTATGGCGGCGTCAACGGCACCGGCTCGTCGATCGATCTGCG
CGGCTTCGGCGCCACCGCGACCTCGAACACACTGTTCCTGGTCAACGGCCGCCGCCTCAACGACCTCGATCTGCAGGGCG
TCGACCTGTCGAGCATCCCGCTGCAATCGATCGAGCGCATCGAGATCACCCGCGGCAGCAGCGGCGCGGTGCTGTACGGC
GACAATGCGGTCGGCGGCGTCATCAATATCGTCACCAAGACCGGCGCCGGCGGACCGCCCGCGACGTTCCGGGCCGAAGG
CGGCTTCGGCTCGTTCAACCAGCGGCTGGCATCGATTTCCGCGGCGTACAATTCCGGGCCGTGGTCGACCTCGGTGTTCG
CCAACGGCGTGCGCTCCAACGGCTATCGCGCCAACAACGCGCTGGAACAGAACAACGCGATCGGCGAGCTGCGCTATGCC
ACGCCGGATCTCAGCGCCTTCGTCAACGTCTCCGGCGACAATCAGCATCTCGGCCTGCCCGGTTCCCGGATCGTGGATCC
CTCGATCGGCGTCAACCAGCTCCAGACCGATCGCCGCGGCACCAACACGCCGTTCGATTTCGGCGACAAGCAGGGCGCCA
ACATCACCGCCGGCTTCACCAAATCGCTGTGGGACGGCGCCGAATTGATCGTCGACGGCGGCGTCCGCGACAAGCGGCAG
CAGGCCGGCTATTTCGGCAATGTGCCGCTGTCGAGCTTCAGCGCGAGCTGGTTCGACGCACATCTGCAGACCTGGTCGAT
CACGCCGCGGCTGAGCATCACCCATGCGATGTTCGGCCTGCCGTCGAAAATCCTGACCGGTCTCGACTATTACGACGCGA
CCTATGATTCCGACCGCGGCCAGTATCGCAGCACGCCGCCGGTGCACGTCTACAACCTGTCGCAGAAGACGCTGGCCGGC
TATTGGCAGCAGACCGTCGGCATCCTGCCGACCACCGACCTGTCCTATGGCGGCCGGCTGCAGAGCGTCAAAGTCGAGGC
CAGCGATCGGCTGAACCCGCTGGCGCCGGGCTATTTCGGCGAAGCGCAGGCGCTGCCGCTGAGCAGCACCGAAACCCAGC
ACGCGCTGCATATCGGCCTCGACCATCGCTTCAACGACGTGTTCAGCGTGTTCGCCCGCGCCGCGCGCGCGTTCCGCACC
CCGAATGTCGACGAGCGGGTCTCGTCGGGACCGTCCTACGACGCGTTCTTCAATCCGATTCCGGGCAACTTCGCGCTGAA
GACCCAGACCTCGGTCGATTTCGAAGCCGGCTTCCGCGTCAAATCGGGGCCGTTCGAGATGCAGACCAGCGCCTACAACA
TGAATCTCAACAACGAGCTTCATTACGACCCGGTCAATTTCTACAACACCAATCTCGACCCGACCCGGCGCTATGGCGGC
GAGACCAGCGCCTCGTTCCGCGTCAGCGATACGCTGCTGCTGCGCGGCGGCGGCGCCTATACCCGCGCGACGTTTCGCGA
GGGCCCGTTCTCCGGCAACGACGTGCCGCTGGTGTCGCGCTATACGGCGAATGCCGGCGTGACCTGGAACGTCTGGCAGA
AATACGTCGTGTTCGATGCGACCGCCCGCTACTGGAGCAGCCGCTATATGGACAGCGACGAGAACAACCTGCAGACCAAG
ATCCCGGCGAACGGCACGGTCGATGTCAAGCTGAGCGGCGCCTACGAGCATTTCTTCTGGTCGGCCAGCGTGATCAATCT
GTTCAACTCGCAATATTACGATTACGCGGTGGCGAGCAGTTTCACGCCGGGACGCTACGGCGCCTATCCGCTGCCGGGAC
GAACCTATCTCGTCAAGGCCGGCGTGACGTTCTGA

Upstream 100 bases:

>100_bases
CCGGTCGGGAATTGCAGGCATTGGCTTCATCGGATGCGCAGGCATGTCGATGAAACCTCGTTCGCTGTGACGTGCCACAA
ACGTCGACCGAGGTTCATCC

Downstream 100 bases:

>100_bases
CACGACATCACGCTTCATCACATCGTGCATGACAGGCCCCGGCAGCAAAGGCTGTTCCGGGGCCTTTTTCGTGCTACACC
CCGCCCTTCCCAATCCACGA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MPSRSDADCRHHSARCRALLTSTILASSLFASVAPASAQDNTLPAINVTATRSYEGIVGTSSTVITADDIAHSPAQTVQE
IIAQTPGVQTRTLYGGVNGTGSSIDLRGFGATATSNTLFLVNGRRLNDLDLQGVDLSSIPLQSIERIEITRGSSGAVLYG
DNAVGGVINIVTKTGAGGPPATFRAEGGFGSFNQRLASISAAYNSGPWSTSVFANGVRSNGYRANNALEQNNAIGELRYA
TPDLSAFVNVSGDNQHLGLPGSRIVDPSIGVNQLQTDRRGTNTPFDFGDKQGANITAGFTKSLWDGAELIVDGGVRDKRQ
QAGYFGNVPLSSFSASWFDAHLQTWSITPRLSITHAMFGLPSKILTGLDYYDATYDSDRGQYRSTPPVHVYNLSQKTLAG
YWQQTVGILPTTDLSYGGRLQSVKVEASDRLNPLAPGYFGEAQALPLSSTETQHALHIGLDHRFNDVFSVFARAARAFRT
PNVDERVSSGPSYDAFFNPIPGNFALKTQTSVDFEAGFRVKSGPFEMQTSAYNMNLNNELHYDPVNFYNTNLDPTRRYGG
ETSASFRVSDTLLLRGGGAYTRATFREGPFSGNDVPLVSRYTANAGVTWNVWQKYVVFDATARYWSSRYMDSDENNLQTK
IPANGTVDVKLSGAYEHFFWSASVINLFNSQYYDYAVASSFTPGRYGAYPLPGRTYLVKAGVTF

Sequences:

>Translated_704_residues
MPSRSDADCRHHSARCRALLTSTILASSLFASVAPASAQDNTLPAINVTATRSYEGIVGTSSTVITADDIAHSPAQTVQE
IIAQTPGVQTRTLYGGVNGTGSSIDLRGFGATATSNTLFLVNGRRLNDLDLQGVDLSSIPLQSIERIEITRGSSGAVLYG
DNAVGGVINIVTKTGAGGPPATFRAEGGFGSFNQRLASISAAYNSGPWSTSVFANGVRSNGYRANNALEQNNAIGELRYA
TPDLSAFVNVSGDNQHLGLPGSRIVDPSIGVNQLQTDRRGTNTPFDFGDKQGANITAGFTKSLWDGAELIVDGGVRDKRQ
QAGYFGNVPLSSFSASWFDAHLQTWSITPRLSITHAMFGLPSKILTGLDYYDATYDSDRGQYRSTPPVHVYNLSQKTLAG
YWQQTVGILPTTDLSYGGRLQSVKVEASDRLNPLAPGYFGEAQALPLSSTETQHALHIGLDHRFNDVFSVFARAARAFRT
PNVDERVSSGPSYDAFFNPIPGNFALKTQTSVDFEAGFRVKSGPFEMQTSAYNMNLNNELHYDPVNFYNTNLDPTRRYGG
ETSASFRVSDTLLLRGGGAYTRATFREGPFSGNDVPLVSRYTANAGVTWNVWQKYVVFDATARYWSSRYMDSDENNLQTK
IPANGTVDVKLSGAYEHFFWSASVINLFNSQYYDYAVASSFTPGRYGAYPLPGRTYLVKAGVTF
>Mature_703_residues
PSRSDADCRHHSARCRALLTSTILASSLFASVAPASAQDNTLPAINVTATRSYEGIVGTSSTVITADDIAHSPAQTVQEI
IAQTPGVQTRTLYGGVNGTGSSIDLRGFGATATSNTLFLVNGRRLNDLDLQGVDLSSIPLQSIERIEITRGSSGAVLYGD
NAVGGVINIVTKTGAGGPPATFRAEGGFGSFNQRLASISAAYNSGPWSTSVFANGVRSNGYRANNALEQNNAIGELRYAT
PDLSAFVNVSGDNQHLGLPGSRIVDPSIGVNQLQTDRRGTNTPFDFGDKQGANITAGFTKSLWDGAELIVDGGVRDKRQQ
AGYFGNVPLSSFSASWFDAHLQTWSITPRLSITHAMFGLPSKILTGLDYYDATYDSDRGQYRSTPPVHVYNLSQKTLAGY
WQQTVGILPTTDLSYGGRLQSVKVEASDRLNPLAPGYFGEAQALPLSSTETQHALHIGLDHRFNDVFSVFARAARAFRTP
NVDERVSSGPSYDAFFNPIPGNFALKTQTSVDFEAGFRVKSGPFEMQTSAYNMNLNNELHYDPVNFYNTNLDPTRRYGGE
TSASFRVSDTLLLRGGGAYTRATFREGPFSGNDVPLVSRYTANAGVTWNVWQKYVVFDATARYWSSRYMDSDENNLQTKI
PANGTVDVKLSGAYEHFFWSASVINLFNSQYYDYAVASSFTPGRYGAYPLPGRTYLVKAGVTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=155, Percent_Identity=36.7741935483871, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786798, Length=143, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790405, Length=157, Percent_Identity=35.6687898089172, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1786344, Length=306, Percent_Identity=26.4705882352941, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 76191; Mature: 76060

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRSDADCRHHSARCRALLTSTILASSLFASVAPASAQDNTLPAINVTATRSYEGIVGT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEECC
SSTVITADDIAHSPAQTVQEIIAQTPGVQTRTLYGGVNGTGSSIDLRGFGATATSNTLFL
CCEEEEEHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEE
VNGRRLNDLDLQGVDLSSIPLQSIERIEITRGSSGAVLYGDNAVGGVINIVTKTGAGGPP
EECCCCCCCCCCCCCCCCCCHHCCEEEEEECCCCCEEEECCCCCCCEEEEEEECCCCCCC
ATFRAEGGFGSFNQRLASISAAYNSGPWSTSVFANGVRSNGYRANNALEQNNAIGELRYA
CEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEECCCCHHCCCCCEEEEEC
TPDLSAFVNVSGDNQHLGLPGSRIVDPSIGVNQLQTDRRGTNTPFDFGDKQGANITAGFT
CCCCEEEEEECCCCCEECCCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCH
KSLWDGAELIVDGGVRDKRQQAGYFGNVPLSSFSASWFDAHLQTWSITPRLSITHAMFGL
HHHCCCCEEEEECCCCHHHHHCCCCCCCCHHHCCCHHHHHEEEEEECCCCEEEHHHHHCC
PSKILTGLDYYDATYDSDRGQYRSTPPVHVYNLSQKTLAGYWQQTVGILPTTDLSYGGRL
CHHHHHCCCHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEE
QSVKVEASDRLNPLAPGYFGEAQALPLSSTETQHALHIGLDHRFNDVFSVFARAARAFRT
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
PNVDERVSSGPSYDAFFNPIPGNFALKTQTSVDFEAGFRVKSGPFEMQTSAYNMNLNNEL
CCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCEEEEEEEEECCCCCE
HYDPVNFYNTNLDPTRRYGGETSASFRVSDTLLLRGGGAYTRATFREGPFSGNDVPLVSR
EECCCCEEECCCCHHHHCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEEE
YTANAGVTWNVWQKYVVFDATARYWSSRYMDSDENNLQTKIPANGTVDVKLSGAYEHFFW
EECCCCEEHHHCEEEEEEECHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCHHHHHH
SASVINLFNSQYYDYAVASSFTPGRYGAYPLPGRTYLVKAGVTF
HHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
PSRSDADCRHHSARCRALLTSTILASSLFASVAPASAQDNTLPAINVTATRSYEGIVGT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEECC
SSTVITADDIAHSPAQTVQEIIAQTPGVQTRTLYGGVNGTGSSIDLRGFGATATSNTLFL
CCEEEEEHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEE
VNGRRLNDLDLQGVDLSSIPLQSIERIEITRGSSGAVLYGDNAVGGVINIVTKTGAGGPP
EECCCCCCCCCCCCCCCCCCHHCCEEEEEECCCCCEEEECCCCCCCEEEEEEECCCCCCC
ATFRAEGGFGSFNQRLASISAAYNSGPWSTSVFANGVRSNGYRANNALEQNNAIGELRYA
CEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEECCCCHHCCCCCEEEEEC
TPDLSAFVNVSGDNQHLGLPGSRIVDPSIGVNQLQTDRRGTNTPFDFGDKQGANITAGFT
CCCCEEEEEECCCCCEECCCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCH
KSLWDGAELIVDGGVRDKRQQAGYFGNVPLSSFSASWFDAHLQTWSITPRLSITHAMFGL
HHHCCCCEEEEECCCCHHHHHCCCCCCCCHHHCCCHHHHHEEEEEECCCCEEEHHHHHCC
PSKILTGLDYYDATYDSDRGQYRSTPPVHVYNLSQKTLAGYWQQTVGILPTTDLSYGGRL
CHHHHHCCCHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEE
QSVKVEASDRLNPLAPGYFGEAQALPLSSTETQHALHIGLDHRFNDVFSVFARAARAFRT
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
PNVDERVSSGPSYDAFFNPIPGNFALKTQTSVDFEAGFRVKSGPFEMQTSAYNMNLNNEL
CCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCEEEEEEEEECCCCCE
HYDPVNFYNTNLDPTRRYGGETSASFRVSDTLLLRGGGAYTRATFREGPFSGNDVPLVSR
EECCCCEEECCCCHHHHCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEEE
YTANAGVTWNVWQKYVVFDATARYWSSRYMDSDENNLQTKIPANGTVDVKLSGAYEHFFW
EECCCCEEHHHCEEEEEEECHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCHHHHHH
SASVINLFNSQYYDYAVASSFTPGRYGAYPLPGRTYLVKAGVTF
HHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA