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The map label for this gene is ygcA [C]

Identifier: 86751561

GI number: 86751561

Start: 5053281

End: 5054519

Strand: Direct

Name: ygcA [C]

Synonym: RPB_4463

Alternate gene names: 86751561

Gene position: 5053281-5054519 (Clockwise)

Preceding gene: 86751560

Following gene: 86751564

Centisome position: 94.78

GC content: 69.25

Gene sequence:

>1239_bases
ATGAACGAACTCATCGCAATCGATCACGTCGGCCACCGCGGCGACGGCGTCAGCCTGGGCCACGGCGGCGCGGTCTATGT
GCCCTATGCGCTCGGCGGCGAGACGATCGAGGCCGAGCCGGTCGCCGGCCATCCCGACCGCCGCAAATTGCGGCGCGTGG
CGGTCGCGAGCCCGCAGCGGATCGATCCGTTCTGCCCGCATTTCGGGATGTGCGGCGGCTGCGCGATCCAGCATTGGCAG
AGCGATGCGTATCAGGCGTGGAAGCGCAATCTCGTGGTCGAAACGCTGGCGCAGGCCGGGATCGACTGTGCCGTCGGTCC
GCTGATCGACGCCCATGGCGCGGGTCGCCGCCGCATCACCCTGCACGCCCGCAAGGGCACCCACGACGTGCTGAAGGTCG
GCTTCAGCGCCGCGGCCAGCCACGACGTGATCGCGATCGATCACTGCCCGATCCTCGACCCGGCGCTCGACGGCGCGATC
GAGGCGGCGTGGGCGCTGGCCGAAGCGCTGACCCTGATCGGCAAGCCGCTCGACATCCAGGTCACCGCCGCCGAGAACGG
GCTCGACGTCGACGTCCGCGGCTCCGGTCCGCTGCCGCCGAAGCTGATCGGCGCGCTGTCGCTGCTCGCTGAAAAGCACC
GACTGGCGCGGCTGACGCGGCACGGCGAGTTGGTGCTGATGCGCACGCCGCCGACGATCGCAATCGGCAGCGCACGCGTC
ACGCTGCCGCCGGGCGCGTTCCTGCAGGCGACCGCGCTCGGCGAACAGACGCTGGCCGAACTGGCGCTCTCCGAGATCAA
GCGCGTGAAGCTGATCGCCGATCTGTTCTGTGGCGTCGGCCCGTTCGCGCTGCGGCTCGCGACCAAGGCGCGGGTGGTCG
CCTATGACAGCGACGCGCCGGCGGTGAACGCGCTGCAGAAGGCCGCGCAGGCCACCTCCGGGCTGAAGCCGATCAAGGCC
GAACCGCGCGATTTGTTCCGACGCCCGCTGGCGCCGCCGGAACTGCTCGATTTCGACGCCGTGCTGTTCGACCCACCGCG
CCAGGGCGCGCAGGCGCAAGCCGCGCAACTAGCGGCAAGCAAAGTGCCGCTGATCATCGCGGTGTCGTGCAACGCGCAGA
CCTTCGCGCGCGATGCAAAGCTCCTGATCGACGGCGGCTATCGGCTGGAGAAGGTGACGCCGGTCGATCAGTTCAAATAC
ACGCCGCACGTCGAATTGGTGGGCGTATTGCGGAAGTAA

Upstream 100 bases:

>100_bases
GCGCGATTCCATCTGCTGACGCGCGCGCCGCCGCCGGTTCCGGCGCAAAGCTGATCACTTCCGGCTTTACGCATCGCGGC
GCGAAGCCTATCGGATCGCA

Downstream 100 bases:

>100_bases
TAACTTCCCGCGCGTCCAGCAACAAGCAACACGCTATCATTCGTCGTTCCGGGGCGCGCATTGCAACCCTCTCCCCGCTT
GCGGGGAGAGGGTGGACCGA

Product: (Uracil-5)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNELIAIDHVGHRGDGVSLGHGGAVYVPYALGGETIEAEPVAGHPDRRKLRRVAVASPQRIDPFCPHFGMCGGCAIQHWQ
SDAYQAWKRNLVVETLAQAGIDCAVGPLIDAHGAGRRRITLHARKGTHDVLKVGFSAAASHDVIAIDHCPILDPALDGAI
EAAWALAEALTLIGKPLDIQVTAAENGLDVDVRGSGPLPPKLIGALSLLAEKHRLARLTRHGELVLMRTPPTIAIGSARV
TLPPGAFLQATALGEQTLAELALSEIKRVKLIADLFCGVGPFALRLATKARVVAYDSDAPAVNALQKAAQATSGLKPIKA
EPRDLFRRPLAPPELLDFDAVLFDPPRQGAQAQAAQLAASKVPLIIAVSCNAQTFARDAKLLIDGGYRLEKVTPVDQFKY
TPHVELVGVLRK

Sequences:

>Translated_412_residues
MNELIAIDHVGHRGDGVSLGHGGAVYVPYALGGETIEAEPVAGHPDRRKLRRVAVASPQRIDPFCPHFGMCGGCAIQHWQ
SDAYQAWKRNLVVETLAQAGIDCAVGPLIDAHGAGRRRITLHARKGTHDVLKVGFSAAASHDVIAIDHCPILDPALDGAI
EAAWALAEALTLIGKPLDIQVTAAENGLDVDVRGSGPLPPKLIGALSLLAEKHRLARLTRHGELVLMRTPPTIAIGSARV
TLPPGAFLQATALGEQTLAELALSEIKRVKLIADLFCGVGPFALRLATKARVVAYDSDAPAVNALQKAAQATSGLKPIKA
EPRDLFRRPLAPPELLDFDAVLFDPPRQGAQAQAAQLAASKVPLIIAVSCNAQTFARDAKLLIDGGYRLEKVTPVDQFKY
TPHVELVGVLRK
>Mature_412_residues
MNELIAIDHVGHRGDGVSLGHGGAVYVPYALGGETIEAEPVAGHPDRRKLRRVAVASPQRIDPFCPHFGMCGGCAIQHWQ
SDAYQAWKRNLVVETLAQAGIDCAVGPLIDAHGAGRRRITLHARKGTHDVLKVGFSAAASHDVIAIDHCPILDPALDGAI
EAAWALAEALTLIGKPLDIQVTAAENGLDVDVRGSGPLPPKLIGALSLLAEKHRLARLTRHGELVLMRTPPTIAIGSARV
TLPPGAFLQATALGEQTLAELALSEIKRVKLIADLFCGVGPFALRLATKARVVAYDSDAPAVNALQKAAQATSGLKPIKA
EPRDLFRRPLAPPELLDFDAVLFDPPRQGAQAQAAQLAASKVPLIIAVSCNAQTFARDAKLLIDGGYRLEKVTPVDQFKY
TPHVELVGVLRK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=425, Percent_Identity=28, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787083, Length=175, Percent_Identity=33.1428571428571, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 43790; Mature: 43790

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELIAIDHVGHRGDGVSLGHGGAVYVPYALGGETIEAEPVAGHPDRRKLRRVAVASPQR
CCCEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCC
IDPFCPHFGMCGGCAIQHWQSDAYQAWKRNLVVETLAQAGIDCAVGPLIDAHGAGRRRIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCCCEEEE
LHARKGTHDVLKVGFSAAASHDVIAIDHCPILDPALDGAIEAAWALAEALTLIGKPLDIQ
EEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VTAAENGLDVDVRGSGPLPPKLIGALSLLAEKHRLARLTRHGELVLMRTPPTIAIGSARV
EEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCEEE
TLPPGAFLQATALGEQTLAELALSEIKRVKLIADLFCGVGPFALRLATKARVVAYDSDAP
ECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEECCCCC
AVNALQKAAQATSGLKPIKAEPRDLFRRPLAPPELLDFDAVLFDPPRQGAQAQAAQLAAS
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHCCCCEEEECCCCCCCHHHHHHHHHC
KVPLIIAVSCNAQTFARDAKLLIDGGYRLEKVTPVDQFKYTPHVELVGVLRK
CCCEEEEEECCCHHHHCCCEEEEECCEEEEECCCHHHCCCCCCHHHHEECCC
>Mature Secondary Structure
MNELIAIDHVGHRGDGVSLGHGGAVYVPYALGGETIEAEPVAGHPDRRKLRRVAVASPQR
CCCEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCC
IDPFCPHFGMCGGCAIQHWQSDAYQAWKRNLVVETLAQAGIDCAVGPLIDAHGAGRRRIT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEECCCCCCEEEE
LHARKGTHDVLKVGFSAAASHDVIAIDHCPILDPALDGAIEAAWALAEALTLIGKPLDIQ
EEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
VTAAENGLDVDVRGSGPLPPKLIGALSLLAEKHRLARLTRHGELVLMRTPPTIAIGSARV
EEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCEEE
TLPPGAFLQATALGEQTLAELALSEIKRVKLIADLFCGVGPFALRLATKARVVAYDSDAP
ECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEECCCCC
AVNALQKAAQATSGLKPIKAEPRDLFRRPLAPPELLDFDAVLFDPPRQGAQAQAAQLAAS
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHCCCCEEEECCCCCCCHHHHHHHHHC
KVPLIIAVSCNAQTFARDAKLLIDGGYRLEKVTPVDQFKYTPHVELVGVLRK
CCCEEEEEECCCHHHHCCCEEEEECCEEEEECCCHHHCCCCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA