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The map label for this gene is mer [H]

Identifier: 86751513

GI number: 86751513

Start: 4999051

End: 5000004

Strand: Reverse

Name: mer [H]

Synonym: RPB_4412

Alternate gene names: 86751513

Gene position: 5000004-4999051 (Counterclockwise)

Preceding gene: 86751514

Following gene: 86751512

Centisome position: 93.78

GC content: 69.92

Gene sequence:

>954_bases
TTGTCTGAACTCTCGATCTCCAGCGACGGCAGAGACGAACCGGCCGTGTTTCGCGACAAGGTCGCGGCCGGCGAAGCCGG
TGGCGCCGACACGATCTGGATCGCCAACCATCTGTTCCTGCGCGATCCGGCGGTGCTCGGCGCGCTGACGCTGTCGGAGA
CGCGGCGCCTCAAGGTCGCGCTGATGGCGGTGAGCCCGCTGACGCAGCATCCCGTGCAGATCGCCATGGCCACGGCGACG
CTGGCGGAACGCTTTCCGGGGCGACTCAAGCTGTGCCTCGGCGTCGGCGCGCCCGCCGACCTCGCGGCGATCGGGGTCGA
CGGCGCCAAGCCGCTGCGGGCGATGCGCGAAGCGCTGCTGCTCGTCCGCGCGCTGCTGTCGGGCGAGACGGTCACGTTCC
AGGGCGAGACGTTTCGCGTCGATCGCCGCCGACTGGCGGCGGCCGGGGCCGAGATTCCGATCGTGCTGGCGGCGTCGGGT
CCGCAGATGCTCGAGCTGGCCGGCGCCGAGGCCGACGGCGTGCTGATCAGCGCCGGCGCCTCGGTGCCGTTCGTGGCGCA
GACGCTGCAGAGCGTCGCGCGCGGCGCCAAGGGGCGCAAGGTCAAGACCTCGGGCCTGGTCTACGCCTCGGTCGACGACG
ACGAGAGGCGTGCCAACGACCGTCTGCGGCGGATTCTCGCGATTCTGCTGCGCGGCTCACATCACAAGACCAATCTCGGT
CTGGCGGGTACCACGCTCGATCAGCAAGGGCTGAACGACGCGGTGCTGGCGGAGGATTGGAGCCGCGCCGAAGCGATGAT
CGGCGACGACATCGTCGCCCGCCATGCCGCCAGCGGAACGCCCGAACAATTGCGCCGGCGGCTCGCCGAGTATCACGCCT
CCGGGCTCGACGAGATCGTCATCGCCGGCGTGCGCGACGACGCGCAGATCAAAGCCATCCTAACATCGTCATGA

Upstream 100 bases:

>100_bases
CACCATCCGCATCCGACCGAGCAGGAGATCCGGCATTATCTGCGCGGCAACATCTGCCGCTGCACCGGCTACATCAAGAT
CCTCGAAGCGGTGAAGAGCC

Downstream 100 bases:

>100_bases
GGAAACGCATGAAGTTCAGTGTCTGTCTGTCGACCGGATTCGAAGGCGTGATGTATCCGATCCCGTTCGCGGGGCCGGAG
GATTTCATCGCCCAGGCGCA

Product: luciferase-like

Products: NA

Alternate protein names: Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase; Methylene-H(4)MPT reductase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSELSISSDGRDEPAVFRDKVAAGEAGGADTIWIANHLFLRDPAVLGALTLSETRRLKVALMAVSPLTQHPVQIAMATAT
LAERFPGRLKLCLGVGAPADLAAIGVDGAKPLRAMREALLLVRALLSGETVTFQGETFRVDRRRLAAAGAEIPIVLAASG
PQMLELAGAEADGVLISAGASVPFVAQTLQSVARGAKGRKVKTSGLVYASVDDDERRANDRLRRILAILLRGSHHKTNLG
LAGTTLDQQGLNDAVLAEDWSRAEAMIGDDIVARHAASGTPEQLRRRLAEYHASGLDEIVIAGVRDDAQIKAILTSS

Sequences:

>Translated_317_residues
MSELSISSDGRDEPAVFRDKVAAGEAGGADTIWIANHLFLRDPAVLGALTLSETRRLKVALMAVSPLTQHPVQIAMATAT
LAERFPGRLKLCLGVGAPADLAAIGVDGAKPLRAMREALLLVRALLSGETVTFQGETFRVDRRRLAAAGAEIPIVLAASG
PQMLELAGAEADGVLISAGASVPFVAQTLQSVARGAKGRKVKTSGLVYASVDDDERRANDRLRRILAILLRGSHHKTNLG
LAGTTLDQQGLNDAVLAEDWSRAEAMIGDDIVARHAASGTPEQLRRRLAEYHASGLDEIVIAGVRDDAQIKAILTSS
>Mature_316_residues
SELSISSDGRDEPAVFRDKVAAGEAGGADTIWIANHLFLRDPAVLGALTLSETRRLKVALMAVSPLTQHPVQIAMATATL
AERFPGRLKLCLGVGAPADLAAIGVDGAKPLRAMREALLLVRALLSGETVTFQGETFRVDRRRLAAAGAEIPIVLAASGP
QMLELAGAEADGVLISAGASVPFVAQTLQSVARGAKGRKVKTSGLVYASVDDDERRANDRLRRILAILLRGSHHKTNLGL
AGTTLDQQGLNDAVLAEDWSRAEAMIGDDIVARHAASGTPEQLRRRLAEYHASGLDEIVIAGVRDDAQIKAILTSS

Specific function: Catalyzes the oxidation of methyl-H(4)MPT to methylene- H(4)MPT [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mer family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR019946 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.5.99.11 [H]

Molecular weight: Translated: 33441; Mature: 33310

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELSISSDGRDEPAVFRDKVAAGEAGGADTIWIANHLFLRDPAVLGALTLSETRRLKVA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECEEEEECCHHHEEEECCCCCEEEEE
LMAVSPLTQHPVQIAMATATLAERFPGRLKLCLGVGAPADLAAIGVDGAKPLRAMREALL
EEEECCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
LVRALLSGETVTFQGETFRVDRRRLAAAGAEIPIVLAASGPQMLELAGAEADGVLISAGA
HHHHHHCCCEEEECCCEEEHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCEEEECCC
SVPFVAQTLQSVARGAKGRKVKTSGLVYASVDDDERRANDRLRRILAILLRGSHHKTNLG
CCHHHHHHHHHHHCCCCCCEEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
LAGTTLDQQGLNDAVLAEDWSRAEAMIGDDIVARHAASGTPEQLRRRLAEYHASGLDEIV
EEECCCCCCCCCCCHHHCCHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
IAGVRDDAQIKAILTSS
EECCCCCCEEEEEEECC
>Mature Secondary Structure 
SELSISSDGRDEPAVFRDKVAAGEAGGADTIWIANHLFLRDPAVLGALTLSETRRLKVA
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECEEEEECCHHHEEEECCCCCEEEEE
LMAVSPLTQHPVQIAMATATLAERFPGRLKLCLGVGAPADLAAIGVDGAKPLRAMREALL
EEEECCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
LVRALLSGETVTFQGETFRVDRRRLAAAGAEIPIVLAASGPQMLELAGAEADGVLISAGA
HHHHHHCCCEEEECCCEEEHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCEEEECCC
SVPFVAQTLQSVARGAKGRKVKTSGLVYASVDDDERRANDRLRRILAILLRGSHHKTNLG
CCHHHHHHHHHHHCCCCCCEEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
LAGTTLDQQGLNDAVLAEDWSRAEAMIGDDIVARHAASGTPEQLRRRLAEYHASGLDEIV
EEECCCCCCCCCCCHHHCCHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
IAGVRDDAQIKAILTSS
EECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]