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The map label for this gene is yfkN [H]

Identifier: 86751471

GI number: 86751471

Start: 4953346

End: 4955082

Strand: Reverse

Name: yfkN [H]

Synonym: RPB_4369

Alternate gene names: 86751471

Gene position: 4955082-4953346 (Counterclockwise)

Preceding gene: 86751472

Following gene: 86751470

Centisome position: 92.94

GC content: 66.72

Gene sequence:

>1737_bases
ATGCAGGGCCGCGTCTGCGCGGACAAGGGAACGGAAACGATGATCTCGCGGCGGGAATTCCTGCAGGCCACAGCGGCCGC
ATCGGCGCTCACGATCGGCAGCGGCCTCGGTCCGATTGGGCGGGTGGCGGCGCAGCAGCGGCTGACGCAGGCCGACATTC
TGAAGTTCGATCCGCTCGGCACGCTGACGCTGCTGCACGTCACCGACATCCACGCCCAGCTGATGCCGCTGCATTTCCGC
GAGCCGTCGGTCAATCTCGGCGTCGGCGAGGTCAAGGGCAAGCCGCCGCATCTGACCGACGCGGAATTCCGCAACTACTT
CCACGTCGCCACCGGATCGCCGGACGCCTTCGCGCTCACCGCCGATGATTTCGTCGCGCTCGCCCGCAATTACGGCCGGA
TGGGCGGCATGGACCGCATCGCCACCTTGGTGAACGCGGTGCGCGCCGAGCGCGGCGCCGACAAGGTGCTGCTGCTCGAC
GGCGGCGACACCTGGCAGGGCAGCTGGACCTCGCTGCAGAGCAAGGGCCAGGACATGATCGACGTCATGACCGCGCTGAA
GCTCGACGCGATGACCGGCCATTGGGAATTCACCTACGGCGCCGAGCGGGTCAAGCAGGTCGCCGACTCGGCGCCGTTCG
CCTTCCTGGCGCAGAACGTCCGCGACAACGAATGGCAGGAGCCGGTGTTCGAGGCGCGCAAGATGTTCGAGCGCGGCGGC
GTCAAGATCGCGGTGATCGGGCAGGCGCTGCCGCGCACCGCGGTCGCCAATCCGCGCTGGATGTTTCCGAACTGGGAGTT
CGGCATCCGCGAGGAGGACATCCAGAAGCAGGCCGACGACGCCCGCGCCGAAGGCGCCGAGGTCGTGGTGCTGCTGTCGC
ACAACGGCTTCGACGTCGACCGCAAGCTCGCCGGCCGGGTCAAGGGCCTCGACATCATCCTCACCGCCCACACCCACGAC
GCGATGCCGGGCCTGATCAAGGTCGGCGACACCGTGCTGGTGGCGTCGGGCTCGCACGGCAAATTCGTGTCGCGGCTCGA
CATCGCGGTGAAGGGCAAGAAAGTGTCCGACATCCGCTTCAAACTGATGCCGGTGTTCGCCGACGCCATCGCGCCGGACC
CGGCGATGAAGCAACTGGTCGAGAAGCTGCGTGCGCCCTACGCCAAGGATCTCGCGCGCGTCGTCGGCAAGACCGATTCG
CTGCTGTATCGCCGCGGCAATTTCAACGGCACCTTCGACGACCTGATCTGCGACGCGATGCTGAAGCAGCGCGACACCGA
GATCGCGCTGTCGCCGGGCTTCCGTTGGGGCGGCACGCTGCTGCCGAACGAGGACATCACCTGGGAGGCGATCACCAACG
CCACCGCGATCACCTATCCGAACTGCTACCGCAGCGAGATGACCGGCGAGCAGCTCAAGAACGTGCTCGAGGACATCGCC
GACAACATCTTCCACCCAGACCCGTATTTCCAGGGCGGCGGCGACATGGTCCGCACCGGCGGCATGGGCTATTCGATCGA
TATCGGCAAGGAGATCGGCTCGCGGATCTCCGGCATGGTGCATCTCAAGACCGGCAAGCCCATCGAGGCGTCGAAGACCT
ACACCGTCTCCGGCTGGGCCAGCATCAACCAGAACACCGAGGGCCCGCCGATCTGGGACGTGCTGGCCAAGCACGTCGCG
CAGGCGGGGCCGGTGAAGATCGATCCCAACAGCGCCGTCAAGGTGTCGGGCGCCTGA

Upstream 100 bases:

>100_bases
GACGGCGCGTCGGCGCTGATTCGCATCGACCGTGGGCGTGCAGCCCGCGGATCGCGGAGTTGCGCCGAATTTTTAGTCGA
GCGGATCGGGCCCGTGCGCA

Downstream 100 bases:

>100_bases
GATGACGACCGCCTCCGACGCTCCCCGACCGCTGCAGTCGCGCATGGTGATAGCAAAAGGCAATCCGGCATGAGTGAGAA
GCCCCCCTCCGACATCCTCG

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MQGRVCADKGTETMISRREFLQATAAASALTIGSGLGPIGRVAAQQRLTQADILKFDPLGTLTLLHVTDIHAQLMPLHFR
EPSVNLGVGEVKGKPPHLTDAEFRNYFHVATGSPDAFALTADDFVALARNYGRMGGMDRIATLVNAVRAERGADKVLLLD
GGDTWQGSWTSLQSKGQDMIDVMTALKLDAMTGHWEFTYGAERVKQVADSAPFAFLAQNVRDNEWQEPVFEARKMFERGG
VKIAVIGQALPRTAVANPRWMFPNWEFGIREEDIQKQADDARAEGAEVVVLLSHNGFDVDRKLAGRVKGLDIILTAHTHD
AMPGLIKVGDTVLVASGSHGKFVSRLDIAVKGKKVSDIRFKLMPVFADAIAPDPAMKQLVEKLRAPYAKDLARVVGKTDS
LLYRRGNFNGTFDDLICDAMLKQRDTEIALSPGFRWGGTLLPNEDITWEAITNATAITYPNCYRSEMTGEQLKNVLEDIA
DNIFHPDPYFQGGGDMVRTGGMGYSIDIGKEIGSRISGMVHLKTGKPIEASKTYTVSGWASINQNTEGPPIWDVLAKHVA
QAGPVKIDPNSAVKVSGA

Sequences:

>Translated_578_residues
MQGRVCADKGTETMISRREFLQATAAASALTIGSGLGPIGRVAAQQRLTQADILKFDPLGTLTLLHVTDIHAQLMPLHFR
EPSVNLGVGEVKGKPPHLTDAEFRNYFHVATGSPDAFALTADDFVALARNYGRMGGMDRIATLVNAVRAERGADKVLLLD
GGDTWQGSWTSLQSKGQDMIDVMTALKLDAMTGHWEFTYGAERVKQVADSAPFAFLAQNVRDNEWQEPVFEARKMFERGG
VKIAVIGQALPRTAVANPRWMFPNWEFGIREEDIQKQADDARAEGAEVVVLLSHNGFDVDRKLAGRVKGLDIILTAHTHD
AMPGLIKVGDTVLVASGSHGKFVSRLDIAVKGKKVSDIRFKLMPVFADAIAPDPAMKQLVEKLRAPYAKDLARVVGKTDS
LLYRRGNFNGTFDDLICDAMLKQRDTEIALSPGFRWGGTLLPNEDITWEAITNATAITYPNCYRSEMTGEQLKNVLEDIA
DNIFHPDPYFQGGGDMVRTGGMGYSIDIGKEIGSRISGMVHLKTGKPIEASKTYTVSGWASINQNTEGPPIWDVLAKHVA
QAGPVKIDPNSAVKVSGA
>Mature_578_residues
MQGRVCADKGTETMISRREFLQATAAASALTIGSGLGPIGRVAAQQRLTQADILKFDPLGTLTLLHVTDIHAQLMPLHFR
EPSVNLGVGEVKGKPPHLTDAEFRNYFHVATGSPDAFALTADDFVALARNYGRMGGMDRIATLVNAVRAERGADKVLLLD
GGDTWQGSWTSLQSKGQDMIDVMTALKLDAMTGHWEFTYGAERVKQVADSAPFAFLAQNVRDNEWQEPVFEARKMFERGG
VKIAVIGQALPRTAVANPRWMFPNWEFGIREEDIQKQADDARAEGAEVVVLLSHNGFDVDRKLAGRVKGLDIILTAHTHD
AMPGLIKVGDTVLVASGSHGKFVSRLDIAVKGKKVSDIRFKLMPVFADAIAPDPAMKQLVEKLRAPYAKDLARVVGKTDS
LLYRRGNFNGTFDDLICDAMLKQRDTEIALSPGFRWGGTLLPNEDITWEAITNATAITYPNCYRSEMTGEQLKNVLEDIA
DNIFHPDPYFQGGGDMVRTGGMGYSIDIGKEIGSRISGMVHLKTGKPIEASKTYTVSGWASINQNTEGPPIWDVLAKHVA
QAGPVKIDPNSAVKVSGA

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=430, Percent_Identity=23.2558139534884, Blast_Score=80, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922444, Length=432, Percent_Identity=23.6111111111111, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221329836, Length=197, Percent_Identity=29.9492385786802, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641187, Length=197, Percent_Identity=29.9492385786802, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573524, Length=208, Percent_Identity=26.4423076923077, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 62986; Mature: 62986

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGRVCADKGTETMISRREFLQATAAASALTIGSGLGPIGRVAAQQRLTQADILKFDPLG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCEEECCCC
TLTLLHVTDIHAQLMPLHFREPSVNLGVGEVKGKPPHLTDAEFRNYFHVATGSPDAFALT
CEEEEEEEHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCEEEEE
ADDFVALARNYGRMGGMDRIATLVNAVRAERGADKVLLLDGGDTWQGSWTSLQSKGQDMI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCHHHH
DVMTALKLDAMTGHWEFTYGAERVKQVADSAPFAFLAQNVRDNEWQEPVFEARKMFERGG
HHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCC
VKIAVIGQALPRTAVANPRWMFPNWEFGIREEDIQKQADDARAEGAEVVVLLSHNGFDVD
CEEEEECCCCCCHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
RKLAGRVKGLDIILTAHTHDAMPGLIKVGDTVLVASGSHGKFVSRLDIAVKGKKVSDIRF
HHHHCCCCCEEEEEEECCCCCCCCCEEECCEEEEEECCCCCEEEEEEEEEECCEECCEEE
KLMPVFADAIAPDPAMKQLVEKLRAPYAKDLARVVGKTDSLLYRRGNFNGTFDDLICDAM
EEHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHEEECCCCCCCHHHHHHHHH
LKQRDTEIALSPGFRWGGTLLPNEDITWEAITNATAITYPNCYRSEMTGEQLKNVLEDIA
HHCCCCEEEECCCCCCCCEECCCCCCCEEEECCCEEEECCCHHHHHCCHHHHHHHHHHHH
DNIFHPDPYFQGGGDMVRTGGMGYSIDIGKEIGSRISGMVHLKTGKPIEASKTYTVSGWA
HHCCCCCCCCCCCCCEEEECCCCEEEECCHHHHHHHCCEEEEECCCCCCCCCEEEEEEEE
SINQNTEGPPIWDVLAKHVAQAGPVKIDPNSAVKVSGA
ECCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEECCC
>Mature Secondary Structure
MQGRVCADKGTETMISRREFLQATAAASALTIGSGLGPIGRVAAQQRLTQADILKFDPLG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCEEECCCC
TLTLLHVTDIHAQLMPLHFREPSVNLGVGEVKGKPPHLTDAEFRNYFHVATGSPDAFALT
CEEEEEEEHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCEEEEE
ADDFVALARNYGRMGGMDRIATLVNAVRAERGADKVLLLDGGDTWQGSWTSLQSKGQDMI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCHHHH
DVMTALKLDAMTGHWEFTYGAERVKQVADSAPFAFLAQNVRDNEWQEPVFEARKMFERGG
HHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCC
VKIAVIGQALPRTAVANPRWMFPNWEFGIREEDIQKQADDARAEGAEVVVLLSHNGFDVD
CEEEEECCCCCCHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
RKLAGRVKGLDIILTAHTHDAMPGLIKVGDTVLVASGSHGKFVSRLDIAVKGKKVSDIRF
HHHHCCCCCEEEEEEECCCCCCCCCEEECCEEEEEECCCCCEEEEEEEEEECCEECCEEE
KLMPVFADAIAPDPAMKQLVEKLRAPYAKDLARVVGKTDSLLYRRGNFNGTFDDLICDAM
EEHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHEEECCCCCCCHHHHHHHHH
LKQRDTEIALSPGFRWGGTLLPNEDITWEAITNATAITYPNCYRSEMTGEQLKNVLEDIA
HHCCCCEEEECCCCCCCCEECCCCCCCEEEECCCEEEECCCHHHHHCCHHHHHHHHHHHH
DNIFHPDPYFQGGGDMVRTGGMGYSIDIGKEIGSRISGMVHLKTGKPIEASKTYTVSGWA
HHCCCCCCCCCCCCCEEEECCCCEEEECCHHHHHHHCCEEEEECCCCCCCCCEEEEEEEE
SINQNTEGPPIWDVLAKHVAQAGPVKIDPNSAVKVSGA
ECCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]