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The map label for this gene is nodL [H]

Identifier: 86751292

GI number: 86751292

Start: 4758405

End: 4758965

Strand: Reverse

Name: nodL [H]

Synonym: RPB_4185

Alternate gene names: 86751292

Gene position: 4758965-4758405 (Counterclockwise)

Preceding gene: 86751293

Following gene: 86751289

Centisome position: 89.26

GC content: 71.12

Gene sequence:

>561_bases
GTGACCCGGACCGAGAAGGAGAAGATGCTGGCGGGCGAGCTGTACCGCCCCGGCAGCCCCGAGCTCGCCGCCGACGAGGC
CGTCAACAAGGCCTGGATCGTGCGCTACAACGCCGCCGGCGCGCTGCCGGCGGTCGAGCGGCATGCGCTTCTGTCGGAGC
ATTTCGGCCATGTCGGCAAGGGCGCCGTCGTCCGCCCGCCGTTCTTCTGCGACTGCGGCTACAACATCCGGCTCGGCGAC
GGCGTGTTTCTGAATTTCAACTGCGTCATCCTCGACATCATGCCGGTGTCGATCGGCGACCGCACCCAGATCGGCCCGGC
GGTGCAGATCTACGCCGCCGACCACCCCCGCGACGCTGCCACCCGCCGCGACGGCCTCGAATTCGGTCGGCCGGTGACGA
TCGGCGCCGACGTCTGGATCGGCGGCGGTTCGATCATCCTGCCGGGCATAACGATCGGCGACGGCGCCGTGATCGGCGCC
GGCAGCGTCGTCACCCGCGACGTCGCCCCGGGGGCGACAGTGGGTGGCAACCCGGCCAGGCCGCTCGGGCGGCGGGACTG
A

Upstream 100 bases:

>100_bases
GCATCGAGGCGCTGGTCCGGCAGATGGACGACGACAGCGCCCGCGCCCGCGCCGCGCTCGCCGCCGACCATGGCGCATTT
CCGAAACTCGGGACGATCGG

Downstream 100 bases:

>100_bases
GAAGGCTGGGGTCAGGTCGCCAACAGGCGGGGATCGAACGGCGCCTTGATGATCTCCGCCACGGTGACGGCAGATGCGTC
GTGATGCGCAGGGTTGAGCA

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTRTEKEKMLAGELYRPGSPELAADEAVNKAWIVRYNAAGALPAVERHALLSEHFGHVGKGAVVRPPFFCDCGYNIRLGD
GVFLNFNCVILDIMPVSIGDRTQIGPAVQIYAADHPRDAATRRDGLEFGRPVTIGADVWIGGGSIILPGITIGDGAVIGA
GSVVTRDVAPGATVGGNPARPLGRRD

Sequences:

>Translated_186_residues
MTRTEKEKMLAGELYRPGSPELAADEAVNKAWIVRYNAAGALPAVERHALLSEHFGHVGKGAVVRPPFFCDCGYNIRLGD
GVFLNFNCVILDIMPVSIGDRTQIGPAVQIYAADHPRDAATRRDGLEFGRPVTIGADVWIGGGSIILPGITIGDGAVIGA
GSVVTRDVAPGATVGGNPARPLGRRD
>Mature_185_residues
TRTEKEKMLAGELYRPGSPELAADEAVNKAWIVRYNAAGALPAVERHALLSEHFGHVGKGAVVRPPFFCDCGYNIRLGDG
VFLNFNCVILDIMPVSIGDRTQIGPAVQIYAADHPRDAATRRDGLEFGRPVTIGADVWIGGGSIILPGITIGDGAVIGAG
SVVTRDVAPGATVGGNPARPLGRRD

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=181, Percent_Identity=49.171270718232, Blast_Score=180, Evalue=4e-47,
Organism=Escherichia coli, GI1786537, Length=180, Percent_Identity=36.6666666666667, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322243, Length=175, Percent_Identity=41.7142857142857, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 19570; Mature: 19438

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTEKEKMLAGELYRPGSPELAADEAVNKAWIVRYNAAGALPAVERHALLSEHFGHVGK
CCCCCHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
GAVVRPPFFCDCGYNIRLGDGVFLNFNCVILDIMPVSIGDRTQIGPAVQIYAADHPRDAA
CCEECCCEEECCCCEEEECCEEEEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCHH
TRRDGLEFGRPVTIGADVWIGGGSIILPGITIGDGAVIGAGSVVTRDVAPGATVGGNPAR
HHHCCHHCCCCEEECCEEEECCCCEEECCEEECCCCEEECCCEEEECCCCCCCCCCCCCC
PLGRRD
CCCCCC
>Mature Secondary Structure 
TRTEKEKMLAGELYRPGSPELAADEAVNKAWIVRYNAAGALPAVERHALLSEHFGHVGK
CCCCHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
GAVVRPPFFCDCGYNIRLGDGVFLNFNCVILDIMPVSIGDRTQIGPAVQIYAADHPRDAA
CCEECCCEEECCCCEEEECCEEEEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCHH
TRRDGLEFGRPVTIGADVWIGGGSIILPGITIGDGAVIGAGSVVTRDVAPGATVGGNPAR
HHHCCHHCCCCEEECCEEEECCCCEEECCEEECCCCEEECCCEEEECCCCCCCCCCCCCC
PLGRRD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1558960; 8801423; 11481432 [H]