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The map label for this gene is alkJ [H]

Identifier: 86751170

GI number: 86751170

Start: 4633199

End: 4634794

Strand: Direct

Name: alkJ [H]

Synonym: RPB_4062

Alternate gene names: 86751170

Gene position: 4633199-4634794 (Clockwise)

Preceding gene: 86751169

Following gene: 86751171

Centisome position: 86.9

GC content: 68.48

Gene sequence:

>1596_bases
ATGGCCGCGCAGGGTTGGGACTACATCATCGTCGGCGCCGGCTCCGCGGGCTGCATCGTCGCCAACCGGCTGTCGGCCGA
TCCGGCCTGCCGCGTGCTGCTGCTCGAGGCCGGCGGCTCGGATCGCAACATCTGGCTGAAGCTGCCGGTCGGCTATTATC
GCTCGATCTACGACGACCGCTTCTCGCGCAAATTCATCACCGAGCCGAGCGACGTCACCGGCGGCCGCGCCATCGTCTGG
CCGCGCGGCCGCGTGCTCGGGGGCTCGTCGTCGATCAACGGGCTGATCTTCATCCGCGGCGAGCCGGCCGGCTTCGACGA
TTGGGAGCGGCTCGGCGCCAAAGGCTGGAGCTATCAGGAGCTGCTGCCGTATTTCCGGCGCTACGAGCGCTATCGCGGCG
GCGACAGCCAGTATCACGGCGGTTTCGGCGAGTTCGAAGTCTCCGATCTGCGCACCGGCAGCGAGGCCGCCGCCGCATGG
GTGCAGGCCGGCATCGAATTCGGCCTGCCGCGCAATCCGGACTTCAACGCGGAGACGACTTACGGCGTCGGCGCCTATCA
GCTCGGCATCGGCCGGCGCTGGCGCTCGAGCTCGGCTTCCGCTTTTCTGCATCCGGTCATGCACCGCACGAATCTGACGG
TGATCACCCGGGCACACGCGAGCCGCGTGCTGTTCGACGGCACCACCGCCACCGGCGTCGAATGGATCAGGGACGGGCAA
CGGATCCAGGCGCGCGCCGAACGTGAAGTAATCCTCTCGGCCGGCGCGCTGCAGTCGCCGCAATTGCTACAGCTCTCCGG
TATCGGCCCTGCGGCGCTGCTGCGCGGCCTCGGCATCGAAATCGTGGCCGATGCGCCCGAGGTCGGGCGCAACCTGCAGG
ATCATTATCAGGCGCGGATGATCGTGCGGCTGAAGCAGAAGCACTCGCTCAACGATCAGGTGCGCAGCCCGGTCGGGCTC
GCGAAGATGGGCCTGCAATGGCTGCTCGCCGGCAACGGGCCGCTCACCGCCGGCGCCGGCCAGGTCGGCGGCGCCGCCTG
CACGCGCTATGCGAAGAACGGCCGCCCCGACGTGCAGTTCAACGTCATGCCGCTGTCGGTCGACAAGCCCGGCGAGCCGC
TGCACAGCTACTCGGGCTTCACCGCTTCGGTGTGGCAGTGCCACGCCGAATCGCGGGGCCATCTGGCGATCCGTTCGACC
GACCCGTTCGAGCAGCCGACCATCGTACCGAACTATTTCGAGCGCGAGATCGATCGTAACACCATCGTCGCCGGGCTCGA
GATCCTGCGCGAGATCTATCGGCAGCCGTCGTTCCGCGAGCGCTGGGACCTCGACGTGGTGCCGGGCGAGAACATCAACG
ACCCTGCCGGGCTGTGGGAGTTCGCCCGCACCACCGGCGGCACGGTGTTCCATGCCTGCGGCACCTGCCGGATGGGTTCC
GACGACGGCGCGGTGGTCGATCCGCGCCTGCGCGTGCGCGGCGTCGAGCGGCTGCGCGTGGTCGACGCCTCGGTGATGCC
ACTGATCACCTCGGCCAATACCAACGCCGCCAGCCTGATGATCGGCGAGAAGGGCGCCGCCCTGATCGCCTCATGA

Upstream 100 bases:

>100_bases
CAGCGACGGCGCCATCACGCTCGGCGATGCGCCCGGCCTCGGAGTCGAGCCGGACCTCGTCGGCATCGCGCAGTATCGAA
CCGTATAGGACGCCGCATCG

Downstream 100 bases:

>100_bases
GGAAGATCAACGCCAGTTCCGACGCGGATCGTCATGCTCACGGTATGGACCTGAGCATCGACAGCCACGTGCCCAAATAC
GCGCAGATCGCCGACATCTT

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MAAQGWDYIIVGAGSAGCIVANRLSADPACRVLLLEAGGSDRNIWLKLPVGYYRSIYDDRFSRKFITEPSDVTGGRAIVW
PRGRVLGGSSSINGLIFIRGEPAGFDDWERLGAKGWSYQELLPYFRRYERYRGGDSQYHGGFGEFEVSDLRTGSEAAAAW
VQAGIEFGLPRNPDFNAETTYGVGAYQLGIGRRWRSSSASAFLHPVMHRTNLTVITRAHASRVLFDGTTATGVEWIRDGQ
RIQARAEREVILSAGALQSPQLLQLSGIGPAALLRGLGIEIVADAPEVGRNLQDHYQARMIVRLKQKHSLNDQVRSPVGL
AKMGLQWLLAGNGPLTAGAGQVGGAACTRYAKNGRPDVQFNVMPLSVDKPGEPLHSYSGFTASVWQCHAESRGHLAIRST
DPFEQPTIVPNYFEREIDRNTIVAGLEILREIYRQPSFRERWDLDVVPGENINDPAGLWEFARTTGGTVFHACGTCRMGS
DDGAVVDPRLRVRGVERLRVVDASVMPLITSANTNAASLMIGEKGAALIAS

Sequences:

>Translated_531_residues
MAAQGWDYIIVGAGSAGCIVANRLSADPACRVLLLEAGGSDRNIWLKLPVGYYRSIYDDRFSRKFITEPSDVTGGRAIVW
PRGRVLGGSSSINGLIFIRGEPAGFDDWERLGAKGWSYQELLPYFRRYERYRGGDSQYHGGFGEFEVSDLRTGSEAAAAW
VQAGIEFGLPRNPDFNAETTYGVGAYQLGIGRRWRSSSASAFLHPVMHRTNLTVITRAHASRVLFDGTTATGVEWIRDGQ
RIQARAEREVILSAGALQSPQLLQLSGIGPAALLRGLGIEIVADAPEVGRNLQDHYQARMIVRLKQKHSLNDQVRSPVGL
AKMGLQWLLAGNGPLTAGAGQVGGAACTRYAKNGRPDVQFNVMPLSVDKPGEPLHSYSGFTASVWQCHAESRGHLAIRST
DPFEQPTIVPNYFEREIDRNTIVAGLEILREIYRQPSFRERWDLDVVPGENINDPAGLWEFARTTGGTVFHACGTCRMGS
DDGAVVDPRLRVRGVERLRVVDASVMPLITSANTNAASLMIGEKGAALIAS
>Mature_530_residues
AAQGWDYIIVGAGSAGCIVANRLSADPACRVLLLEAGGSDRNIWLKLPVGYYRSIYDDRFSRKFITEPSDVTGGRAIVWP
RGRVLGGSSSINGLIFIRGEPAGFDDWERLGAKGWSYQELLPYFRRYERYRGGDSQYHGGFGEFEVSDLRTGSEAAAAWV
QAGIEFGLPRNPDFNAETTYGVGAYQLGIGRRWRSSSASAFLHPVMHRTNLTVITRAHASRVLFDGTTATGVEWIRDGQR
IQARAEREVILSAGALQSPQLLQLSGIGPAALLRGLGIEIVADAPEVGRNLQDHYQARMIVRLKQKHSLNDQVRSPVGLA
KMGLQWLLAGNGPLTAGAGQVGGAACTRYAKNGRPDVQFNVMPLSVDKPGEPLHSYSGFTASVWQCHAESRGHLAIRSTD
PFEQPTIVPNYFEREIDRNTIVAGLEILREIYRQPSFRERWDLDVVPGENINDPAGLWEFARTTGGTVFHACGTCRMGSD
DGAVVDPRLRVRGVERLRVVDASVMPLITSANTNAASLMIGEKGAALIAS

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=535, Percent_Identity=39.6261682242991, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1786503, Length=533, Percent_Identity=38.2739212007505, Blast_Score=343, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17532301, Length=546, Percent_Identity=35.3479853479854, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24642042, Length=565, Percent_Identity=36.6371681415929, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24642059, Length=570, Percent_Identity=35.0877192982456, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24642048, Length=564, Percent_Identity=36.1702127659575, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI45549471, Length=574, Percent_Identity=35.191637630662, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI45551458, Length=570, Percent_Identity=35.2631578947368, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24650267, Length=566, Percent_Identity=34.982332155477, Blast_Score=279, Evalue=4e-75,
Organism=Drosophila melanogaster, GI18859995, Length=562, Percent_Identity=36.1209964412811, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642055, Length=571, Percent_Identity=33.9754816112084, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24642035, Length=571, Percent_Identity=34.8511383537653, Blast_Score=268, Evalue=1e-71,
Organism=Drosophila melanogaster, GI17137792, Length=551, Percent_Identity=34.1197822141561, Blast_Score=267, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642051, Length=568, Percent_Identity=32.7464788732394, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24642037, Length=585, Percent_Identity=34.1880341880342, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24642039, Length=570, Percent_Identity=33.1578947368421, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI18859993, Length=571, Percent_Identity=32.7495621716287, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24642057, Length=582, Percent_Identity=30.5841924398625, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=27.319587628866, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 57956; Mature: 57825

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQGWDYIIVGAGSAGCIVANRLSADPACRVLLLEAGGSDRNIWLKLPVGYYRSIYDDR
CCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHH
FSRKFITEPSDVTGGRAIVWPRGRVLGGSSSINGLIFIRGEPAGFDDWERLGAKGWSYQE
HHHHHCCCCCCCCCCEEEEECCCEEECCCCCCCEEEEEECCCCCCCHHHHHCCCCCCHHH
LLPYFRRYERYRGGDSQYHGGFGEFEVSDLRTGSEAAAAWVQAGIEFGLPRNPDFNAETT
HHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
YGVGAYQLGIGRRWRSSSASAFLHPVMHRTNLTVITRAHASRVLFDGTTATGVEWIRDGQ
CCCCEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEECCCCHHHHHHHHCCH
RIQARAEREVILSAGALQSPQLLQLSGIGPAALLRGLGIEIVADAPEVGRNLQDHYQARM
HHHHHHHHHEEEECCCCCCCCEEEECCCCHHHHHHCCCEEEEECCHHHCCCHHHHHHHHH
IVRLKQKHSLNDQVRSPVGLAKMGLQWLLAGNGPLTAGAGQVGGAACTRYAKNGRPDVQF
HHHHHHHCCCCHHHHCCCCHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
NVMPLSVDKPGEPLHSYSGFTASVWQCHAESRGHLAIRSTDPFEQPTIVPNYFEREIDRN
EEEEEECCCCCCCHHHCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHCCCH
TIVAGLEILREIYRQPSFRERWDLDVVPGENINDPAGLWEFARTTGGTVFHACGTCRMGS
HHHHHHHHHHHHHHCCCCCHHCCEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCC
DDGAVVDPRLRVRGVERLRVVDASVMPLITSANTNAASLMIGEKGAALIAS
CCCCEECCHHHHCCCHHEEEECCCCEEEEEECCCCCEEEEECCCCCEEECC
>Mature Secondary Structure 
AAQGWDYIIVGAGSAGCIVANRLSADPACRVLLLEAGGSDRNIWLKLPVGYYRSIYDDR
CCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHH
FSRKFITEPSDVTGGRAIVWPRGRVLGGSSSINGLIFIRGEPAGFDDWERLGAKGWSYQE
HHHHHCCCCCCCCCCEEEEECCCEEECCCCCCCEEEEEECCCCCCCHHHHHCCCCCCHHH
LLPYFRRYERYRGGDSQYHGGFGEFEVSDLRTGSEAAAAWVQAGIEFGLPRNPDFNAETT
HHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
YGVGAYQLGIGRRWRSSSASAFLHPVMHRTNLTVITRAHASRVLFDGTTATGVEWIRDGQ
CCCCEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEECCCCHHHHHHHHCCH
RIQARAEREVILSAGALQSPQLLQLSGIGPAALLRGLGIEIVADAPEVGRNLQDHYQARM
HHHHHHHHHEEEECCCCCCCCEEEECCCCHHHHHHCCCEEEEECCHHHCCCHHHHHHHHH
IVRLKQKHSLNDQVRSPVGLAKMGLQWLLAGNGPLTAGAGQVGGAACTRYAKNGRPDVQF
HHHHHHHCCCCHHHHCCCCHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
NVMPLSVDKPGEPLHSYSGFTASVWQCHAESRGHLAIRSTDPFEQPTIVPNYFEREIDRN
EEEEEECCCCCCCHHHCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHCCCH
TIVAGLEILREIYRQPSFRERWDLDVVPGENINDPAGLWEFARTTGGTVFHACGTCRMGS
HHHHHHHHHHHHHHCCCCCHHCCEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCC
DDGAVVDPRLRVRGVERLRVVDASVMPLITSANTNAASLMIGEKGAALIAS
CCCCEECCHHHHCCCHHEEEECCCCEEEEEECCCCCEEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]