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The map label for this gene is yitF [H]

Identifier: 86751169

GI number: 86751169

Start: 4632059

End: 4633186

Strand: Direct

Name: yitF [H]

Synonym: RPB_4061

Alternate gene names: 86751169

Gene position: 4632059-4633186 (Clockwise)

Preceding gene: 86751165

Following gene: 86751170

Centisome position: 86.88

GC content: 69.59

Gene sequence:

>1128_bases
ATGACCGCGCAGGATTTCACCATCCGCAGCATCGAGGCGATGTGTTTCCGCTATCCGTTGTCGCTGCCGGTTGTGACCTC
ATTCGGGCGGATGACGACCCGCCCCGCGGTTTTCATCCGTGTCACGGATGAAGACGGCATCAGCGGCTGGGGCGAGGCGT
GGGCGAACTTTCCCGCCACCGGAGCCGAGCATCGCGCCCGAACCATCAACGAGGTGCTGGCGCCGCTCGCGGCGGGCGCC
CGCATCGCGCATCCGTCCGAATTCTTCGAGGCGCTGACGCAGCGCACGGCTGTGCTGGCGCTGCAATCCGGCGAGCAAGG
GCCGTTCGCGCAGGCGATCGCCGGCATCGATCTGGCGCTGTGGGATCTGTTCGCGCGACGCCGCGCCACGCCGCTGTGGC
GGCTGCTCGGCGGCGCGAATGCCACGATCAAGGTCTATGCCAGCGGCATCAATCCCACCGGCACGCGGGAGATGGCCGAA
ACCGCGCTGGCGCGCGGCCATCGCGCGCTCAAGCTGAAGATCGGCTTCGGCGCCGAGATCGATCACCCCAATCTCGCCGC
GCTGCGCGCGCTGGCCGGCGACGGCACGCTGGCCGCCGACGCCAATCAGGCCTGGACGCTGCAGCAGGCATGCGAGGCGG
CACCGCATTTGCGCGACTACAATCTCGCCTGGCTGGAGGAGCCGATCCGCGCCGATCGTCCGTGGCCGGAATGGCAGGCG
CTGCGCCGCGCCGCCACGATGCCGCTCGCCGCCGGCGAGAATTTCGCGAGCCGCGAGAGCTTTCAGCAAGCGCTGTCCGA
CGACACGCTCGGCGTCATCCAGCCCGATATCGCCAAATGGGGCGGGCTGTCGGCCTGCGCGCCGATCGCCCGCGACATCG
TGGCCGCCGGCAAGCGGTTCTGCCCGCATTATCTCGGCGGCGGCATCGGTCTGCTGGCATCGGCGCATCTGCTCGCCGGC
ATCGGCGGTGACGGCCTGCTGGAGGTCGACGCCAACGACAATCCGCTGCGCGAAGCGTTCTGTGGCCCCGTCGCCGCGAT
CAGCGACGGCGCCATCACGCTCGGCGATGCGCCCGGCCTCGGAGTCGAGCCGGACCTCGTCGGCATCGCGCAGTATCGAA
CCGTATAG

Upstream 100 bases:

>100_bases
GTGCGTTGTCCGTCATCTTTGCGTTGATCGGTTGGCGAAGTCATACTATAAATAGGATGACCATACAAGAGGCGGCGGAT
GCGCCGCGGAACGGGACGCG

Downstream 100 bases:

>100_bases
GACGCCGCATCGATGGCCGCGCAGGGTTGGGACTACATCATCGTCGGCGCCGGCTCCGCGGGCTGCATCGTCGCCAACCG
GCTGTCGGCCGATCCGGCCT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTAQDFTIRSIEAMCFRYPLSLPVVTSFGRMTTRPAVFIRVTDEDGISGWGEAWANFPATGAEHRARTINEVLAPLAAGA
RIAHPSEFFEALTQRTAVLALQSGEQGPFAQAIAGIDLALWDLFARRRATPLWRLLGGANATIKVYASGINPTGTREMAE
TALARGHRALKLKIGFGAEIDHPNLAALRALAGDGTLAADANQAWTLQQACEAAPHLRDYNLAWLEEPIRADRPWPEWQA
LRRAATMPLAAGENFASRESFQQALSDDTLGVIQPDIAKWGGLSACAPIARDIVAAGKRFCPHYLGGGIGLLASAHLLAG
IGGDGLLEVDANDNPLREAFCGPVAAISDGAITLGDAPGLGVEPDLVGIAQYRTV

Sequences:

>Translated_375_residues
MTAQDFTIRSIEAMCFRYPLSLPVVTSFGRMTTRPAVFIRVTDEDGISGWGEAWANFPATGAEHRARTINEVLAPLAAGA
RIAHPSEFFEALTQRTAVLALQSGEQGPFAQAIAGIDLALWDLFARRRATPLWRLLGGANATIKVYASGINPTGTREMAE
TALARGHRALKLKIGFGAEIDHPNLAALRALAGDGTLAADANQAWTLQQACEAAPHLRDYNLAWLEEPIRADRPWPEWQA
LRRAATMPLAAGENFASRESFQQALSDDTLGVIQPDIAKWGGLSACAPIARDIVAAGKRFCPHYLGGGIGLLASAHLLAG
IGGDGLLEVDANDNPLREAFCGPVAAISDGAITLGDAPGLGVEPDLVGIAQYRTV
>Mature_374_residues
TAQDFTIRSIEAMCFRYPLSLPVVTSFGRMTTRPAVFIRVTDEDGISGWGEAWANFPATGAEHRARTINEVLAPLAAGAR
IAHPSEFFEALTQRTAVLALQSGEQGPFAQAIAGIDLALWDLFARRRATPLWRLLGGANATIKVYASGINPTGTREMAET
ALARGHRALKLKIGFGAEIDHPNLAALRALAGDGTLAADANQAWTLQQACEAAPHLRDYNLAWLEEPIRADRPWPEWQAL
RRAATMPLAAGENFASRESFQQALSDDTLGVIQPDIAKWGGLSACAPIARDIVAAGKRFCPHYLGGGIGLLASAHLLAGI
GGDGLLEVDANDNPLREAFCGPVAAISDGAITLGDAPGLGVEPDLVGIAQYRTV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=220, Percent_Identity=30.4545454545455, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994953, Length=298, Percent_Identity=28.8590604026846, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39849; Mature: 39717

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQDFTIRSIEAMCFRYPLSLPVVTSFGRMTTRPAVFIRVTDEDGISGWGEAWANFPAT
CCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCC
GAEHRARTINEVLAPLAAGARIAHPSEFFEALTQRTAVLALQSGEQGPFAQAIAGIDLAL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHH
WDLFARRRATPLWRLLGGANATIKVYASGINPTGTREMAETALARGHRALKLKIGFGAEI
HHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
DHPNLAALRALAGDGTLAADANQAWTLQQACEAAPHLRDYNLAWLEEPIRADRPWPEWQA
CCCCHHHHHHHCCCCCEECCCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHH
LRRAATMPLAAGENFASRESFQQALSDDTLGVIQPDIAKWGGLSACAPIARDIVAAGKRF
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
CPHYLGGGIGLLASAHLLAGIGGDGLLEVDANDNPLREAFCGPVAAISDGAITLGDAPGL
CHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCHHCCCCCCEEECCCCCC
GVEPDLVGIAQYRTV
CCCCCCEEHHHHCCC
>Mature Secondary Structure 
TAQDFTIRSIEAMCFRYPLSLPVVTSFGRMTTRPAVFIRVTDEDGISGWGEAWANFPAT
CCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCC
GAEHRARTINEVLAPLAAGARIAHPSEFFEALTQRTAVLALQSGEQGPFAQAIAGIDLAL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHH
WDLFARRRATPLWRLLGGANATIKVYASGINPTGTREMAETALARGHRALKLKIGFGAEI
HHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
DHPNLAALRALAGDGTLAADANQAWTLQQACEAAPHLRDYNLAWLEEPIRADRPWPEWQA
CCCCHHHHHHHCCCCCEECCCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHH
LRRAATMPLAAGENFASRESFQQALSDDTLGVIQPDIAKWGGLSACAPIARDIVAAGKRF
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
CPHYLGGGIGLLASAHLLAGIGGDGLLEVDANDNPLREAFCGPVAAISDGAITLGDAPGL
CHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCHHCCCCCCEEECCCCCC
GVEPDLVGIAQYRTV
CCCCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]