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The map label for this gene is benA [H]

Identifier: 86751003

GI number: 86751003

Start: 4450910

End: 4452181

Strand: Direct

Name: benA [H]

Synonym: RPB_3895

Alternate gene names: 86751003

Gene position: 4450910-4452181 (Clockwise)

Preceding gene: 86751002

Following gene: 86751004

Centisome position: 83.48

GC content: 63.92

Gene sequence:

>1272_bases
ATGACCAGCCTGGCGGAGCTCTCCACCAATCCCGACGACCTGATCCGCAAGGATCGCGTTCGCACCCCGCTCTACACCGA
TCCGGCGATCTTCGACGCCGAGATGACCAAGATCTTCCGCAATACATGGGTCTGGGTCGCGCATGTCAGCGAGATCGCCA
ACAACGGCGACTTCAAGATGGCGCAGGTCGGGCTCGAGCCGGTGATCGTGGTGCGCGACCGCAAGGGCAAGGTCCACGTC
CACCTCAACCGCTGCCGGCATCGCGGCGCGACGCTGTGCGAAGTGCGCAAGGGCAAGACCTCCAGCTTGGTCTGCCCGTA
TCACGGCTGGGGCTATTCGCTCGACGGCAAGCTGCGCGGCGTGCCTTACGAGAACGGCTACGACGAGACGACGCTCGACC
GCAACGAGCTGTCTTTGAAGTCGCTGCGGGTCGAGGAATATAACGGCCTGATCTTCGCGACCTTCCGCGACGACATCGAG
CCCCTGGCCGACTTCCTCGGGCCGGCGAAGAAGTGGATCGACCTGTTCATGAAGCAGGGCGGCGGGTTTCCGGTGAAGGT
GCTCGGCGAACACAAGTTCCGCTTTCCCGGCAACTGGAAGATCCAGCTCGAGAACACCACCGACGCCTATCACTTCCCGC
TGGTGCACAAATCCTTCCTCACCGCGGTCGACAAGGAAACCGAGCAGCAGCTCGACATGCTGAACAATGGCGGCTTCGTC
GAGGATCTCGGCAACGGCCACAGCGTGATGGTGATGATCCCCGAACTGATCGACCTCGACGACAATCTCGAGGCGCCGAT
CCCGGAGAAGTTCGCCGATCTCGCCAAGGAGCTCGTCGCCGAAGGCTATTCCGACACGGAAGTCCGGCGCATCGTCCGCG
CCGCCGGCGGCTCCGGCTTCAACATCAATCTGTTTCCGAATCTGGCCTGCTCGATGGCGTTCTTCCGCGTGCTGCAGCCG
GTCTCGGTGAACGAGACCGAGATCCGCCACATCGCGATCGGCATGGACGGCGGGCCGGCGGCGGCCAACCGCGCGCGGAT
CCGCCTGCACGAATTCTTCCAGGGCCCGATGGGCTTCGGCAGCCCCGACGACGCTGAGGTGTGGGATCGCGTCCAGCACG
GCGCGCAGGGCGGCGACGAGATGTGGATCATGCTGAACCGCGGTTTTCCAAAGGAGGAAAACCTGCCCGACGGGCGCGCC
CGCAGCGACGTCAGCGCCGAAACCGGGATGCGCGCCGCCTATGATCAATGGAAGCGGATGATGACGGTCTGA

Upstream 100 bases:

>100_bases
CAGAGCGCCGGCCGCGTGCTGCTCGGGCTGCCCTCGGTCCCCGGCTATCCATGATGCCGCGCCCCTCTCCGACCATTTCC
CAGCGACAAGGATCAGACAC

Downstream 100 bases:

>100_bases
CCATGAACATGCCCATGACAATCCCCGCGACCACCTCCCTGCCCGTCACGCTCGCCGAGGCGATGGAATTCGCCTGGACC
GAGGCCGATCTGCTCGACCA

Product: ring hydroxylating dioxygenase, Rieske (2Fe-2S) protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTSLAELSTNPDDLIRKDRVRTPLYTDPAIFDAEMTKIFRNTWVWVAHVSEIANNGDFKMAQVGLEPVIVVRDRKGKVHV
HLNRCRHRGATLCEVRKGKTSSLVCPYHGWGYSLDGKLRGVPYENGYDETTLDRNELSLKSLRVEEYNGLIFATFRDDIE
PLADFLGPAKKWIDLFMKQGGGFPVKVLGEHKFRFPGNWKIQLENTTDAYHFPLVHKSFLTAVDKETEQQLDMLNNGGFV
EDLGNGHSVMVMIPELIDLDDNLEAPIPEKFADLAKELVAEGYSDTEVRRIVRAAGGSGFNINLFPNLACSMAFFRVLQP
VSVNETEIRHIAIGMDGGPAAANRARIRLHEFFQGPMGFGSPDDAEVWDRVQHGAQGGDEMWIMLNRGFPKEENLPDGRA
RSDVSAETGMRAAYDQWKRMMTV

Sequences:

>Translated_423_residues
MTSLAELSTNPDDLIRKDRVRTPLYTDPAIFDAEMTKIFRNTWVWVAHVSEIANNGDFKMAQVGLEPVIVVRDRKGKVHV
HLNRCRHRGATLCEVRKGKTSSLVCPYHGWGYSLDGKLRGVPYENGYDETTLDRNELSLKSLRVEEYNGLIFATFRDDIE
PLADFLGPAKKWIDLFMKQGGGFPVKVLGEHKFRFPGNWKIQLENTTDAYHFPLVHKSFLTAVDKETEQQLDMLNNGGFV
EDLGNGHSVMVMIPELIDLDDNLEAPIPEKFADLAKELVAEGYSDTEVRRIVRAAGGSGFNINLFPNLACSMAFFRVLQP
VSVNETEIRHIAIGMDGGPAAANRARIRLHEFFQGPMGFGSPDDAEVWDRVQHGAQGGDEMWIMLNRGFPKEENLPDGRA
RSDVSAETGMRAAYDQWKRMMTV
>Mature_422_residues
TSLAELSTNPDDLIRKDRVRTPLYTDPAIFDAEMTKIFRNTWVWVAHVSEIANNGDFKMAQVGLEPVIVVRDRKGKVHVH
LNRCRHRGATLCEVRKGKTSSLVCPYHGWGYSLDGKLRGVPYENGYDETTLDRNELSLKSLRVEEYNGLIFATFRDDIEP
LADFLGPAKKWIDLFMKQGGGFPVKVLGEHKFRFPGNWKIQLENTTDAYHFPLVHKSFLTAVDKETEQQLDMLNNGGFVE
DLGNGHSVMVMIPELIDLDDNLEAPIPEKFADLAKELVAEGYSDTEVRRIVRAAGGSGFNINLFPNLACSMAFFRVLQPV
SVNETEIRHIAIGMDGGPAAANRARIRLHEFFQGPMGFGSPDDAEVWDRVQHGAQGGDEMWIMLNRGFPKEENLPDGRAR
SDVSAETGMRAAYDQWKRMMTV

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=444, Percent_Identity=27.4774774774775, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788103, Length=201, Percent_Identity=29.3532338308458, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 47633; Mature: 47502

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLAELSTNPDDLIRKDRVRTPLYTDPAIFDAEMTKIFRNTWVWVAHVSEIANNGDFKM
CCCCHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEHHHHHCCCCEEE
AQVGLEPVIVVRDRKGKVHVHLNRCRHRGATLCEVRKGKTSSLVCPYHGWGYSLDGKLRG
EEECCCEEEEEECCCCEEEEEEHHHHCCCCCEEEECCCCCCCEECCCCCCCEEECCEEEC
VPYENGYDETTLDRNELSLKSLRVEEYNGLIFATFRDDIEPLADFLGPAKKWIDLFMKQG
CCCCCCCCCCCCCCCHHHHHHEEEEHHCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHCC
GGFPVKVLGEHKFRFPGNWKIQLENTTDAYHFPLVHKSFLTAVDKETEQQLDMLNNGGFV
CCCEEEEECCCEEECCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EDLGNGHSVMVMIPELIDLDDNLEAPIPEKFADLAKELVAEGYSDTEVRRIVRAAGGSGF
EECCCCCEEEEEECHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCE
NINLFPNLACSMAFFRVLQPVSVNETEIRHIAIGMDGGPAAANRARIRLHEFFQGPMGFG
EEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHEEHHHHHCCCCCCC
SPDDAEVWDRVQHGAQGGDEMWIMLNRGFPKEENLPDGRARSDVSAETGMRAAYDQWKRM
CCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHH
MTV
HCC
>Mature Secondary Structure 
TSLAELSTNPDDLIRKDRVRTPLYTDPAIFDAEMTKIFRNTWVWVAHVSEIANNGDFKM
CCCHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEHHHHHCCCCEEE
AQVGLEPVIVVRDRKGKVHVHLNRCRHRGATLCEVRKGKTSSLVCPYHGWGYSLDGKLRG
EEECCCEEEEEECCCCEEEEEEHHHHCCCCCEEEECCCCCCCEECCCCCCCEEECCEEEC
VPYENGYDETTLDRNELSLKSLRVEEYNGLIFATFRDDIEPLADFLGPAKKWIDLFMKQG
CCCCCCCCCCCCCCCHHHHHHEEEEHHCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHCC
GGFPVKVLGEHKFRFPGNWKIQLENTTDAYHFPLVHKSFLTAVDKETEQQLDMLNNGGFV
CCCEEEEECCCEEECCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EDLGNGHSVMVMIPELIDLDDNLEAPIPEKFADLAKELVAEGYSDTEVRRIVRAAGGSGF
EECCCCCEEEEEECHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCE
NINLFPNLACSMAFFRVLQPVSVNETEIRHIAIGMDGGPAAANRARIRLHEFFQGPMGFG
EEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHEEHHHHHCCCCCCC
SPDDAEVWDRVQHGAQGGDEMWIMLNRGFPKEENLPDGRARSDVSAETGMRAAYDQWKRM
CCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHH
MTV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]