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The map label for this gene is hcl [H]

Identifier: 86750687

GI number: 86750687

Start: 4100846

End: 4102738

Strand: Reverse

Name: hcl [H]

Synonym: RPB_3577

Alternate gene names: 86750687

Gene position: 4102738-4100846 (Counterclockwise)

Preceding gene: 86750688

Following gene: 86750686

Centisome position: 76.95

GC content: 67.35

Gene sequence:

>1893_bases
ATGGGCGCCATGACCAACCCGACTGCGCGCGCCGGCGACGTGGAGCACGGCGAGCCTCATCCGCTACGCGACATTTCGTT
CGGCGACATCGGGATCGCCACCGAACGCCGCCCCGACGGCACGATCTATGTCCGTTCGACCACGACGCTCACCGACTATC
CGGTGCGGATCACCGACCGGCTGCATCATTTCGCCGAAACCGCGCCCGACCGCGTGTTCATGGCGGAGCGCGACGGTTCG
GGCGGCTGGCGCAAGATCACTTACGCCGGAATGCTCGGCGCAGCGCGCAAGATCGCGTCCGCGCTGCTGGCGCGCGGGCT
GTCGGCCGAGCGGCCGGTGATGATCCTGTCGGGCAATTCGATCGACCACGCGCAAATGATGTTCGGCGCGCTGTATGCCG
GTGTCGCGATGTGTCCGGTGTCGCCGCCTTACTCGCTGGTGTCGAAGGATTTCGGCAAGCTGCGCTACATCGTCGGCCTG
CTCACGCCGGGGCTGATCTTCGCCGACGACGTCACCCCGTTCGCGCCGGCGATCCTCGCCACCGTGCCGAGCGATGTCGA
GATCGCGGCGACGCGCGGCGCTGTCGAAGGCCGCCGCGTCACCTCGCTCGACGAGTTGCTGAATACGCCCGAGCATCCCG
GGCTCGCCACGGCGCATGATGCGATCGGCCACGACACCATCGCCAAATTCCTTCTCACCTCGGGCTCGACCGGCAATCCC
AAGGCGGTGATCAACACCCAGCGGATGATCTGCGCCAACCAGGTGATGATCCGCGAGGCGATGGCGTTCCTCAAGGACGA
GCCGCCGGTGATCGTCGACTGGCTGCCGTGGAATCACACCTTCGGCGGCAACCACAATATCGGGCTGACGCTGTTCAACG
GCGGCTCGATGTATATCGACGACGGCAAGCCGACGCCGGCCGGCATCGCGGCGACGATCCGCAACCTGCGCGAGATCGCG
CCCACGGTGTATTTCAACGTCCCGAAAGGTTACGAATCGCTGCTGCCGGTGCTGCGCGAGGACGCGCAGTTGCGGAAGAT
GTTCTTCAGCCGGCTGCACGCGATGTTCTTCTCAGGGGCCAGTCTGGCCGCCCATGTCTGGAAAGGGCTCGACGAGGTGG
GCGCGCAGGAGACCGGCGCGCGGGTGCCGATGCTGACCGGCCTCGGCGCCACCGAGACCGCGCCGTTCTTCATGTCGGTG
ACGCCGCAGACCTCACGTTCGGGGCATGTCGGCCTGCCGGTGCCGGGCAACGAGGCCAAGCTGGTGCCGAACAACGGCAA
GCTGGAGGTCCGCGCCCGCGGACCCAACATCACGCCGGGCTACTGGCGCGCCCCCGAACTCACCGCCAAGGCGTTCGACG
AGGAGGGCTTCTACAAGCTCAACGACGCGCTGAAGCCGGTCGATCCCAACGATCTCAGCGCCGGCTTCGATTTCGACGGT
CGCATCTCCGAGGACTTCAAGCTCGCCTCGGGCACCTGGGTCAGTGTCGGGCCGCTGCGCGCGAAATTGATCGCCGCCTG
CGCGCCGCTGGTGCGCGACGTGGTGATCGCGGGGCTGGATCGCGACACCGTGTCGGCCCTGGCGATCCTCGATGCCGACG
GCTGCAAGCTGATCAACCCGACGCTGCAGCTCGACGATCTCGCCGGCATGGCGGCGGACGATCTGATCCGCGATGCGTTT
CGCGAACGGCTCGGACGGCTGCTGTCGCAGGCGACCGGCTCGTCGAACCGCATCACCCGCGCTGTGCTGCTGGACGAGCC
TTTGTCGATCGACAAGGGCGAGGTCACCGACAAGGGCTCGGTCAACCAGCGCGCCGTGCTGGAGCATCGCGCGGCGCTGA
TCGCCGACCTCTATGCCGAGCCGCCGCCGGCGCATGTGATCGCGGTGGGGTAG

Upstream 100 bases:

>100_bases
GAATCGCTGATGGCGACGGTGGCGCAGAGCGACCAAGAGGCCAAGAACCGGATTCGCGACTTCCTCGATCACAAGACCGC
AAAGGTTAAACCGACCTGAG

Downstream 100 bases:

>100_bases
GGCGGACTGTATGGCCTCAGTGGTTGTCATTCCGGGGCGCGCGCAGCGCGAACCCGGAATCCATAACCCCTGCAGGGAGT
ATGGATTCCGGGCCCGCACC

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: 3-hydroxybenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MGAMTNPTARAGDVEHGEPHPLRDISFGDIGIATERRPDGTIYVRSTTTLTDYPVRITDRLHHFAETAPDRVFMAERDGS
GGWRKITYAGMLGAARKIASALLARGLSAERPVMILSGNSIDHAQMMFGALYAGVAMCPVSPPYSLVSKDFGKLRYIVGL
LTPGLIFADDVTPFAPAILATVPSDVEIAATRGAVEGRRVTSLDELLNTPEHPGLATAHDAIGHDTIAKFLLTSGSTGNP
KAVINTQRMICANQVMIREAMAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYIDDGKPTPAGIAATIRNLREIA
PTVYFNVPKGYESLLPVLREDAQLRKMFFSRLHAMFFSGASLAAHVWKGLDEVGAQETGARVPMLTGLGATETAPFFMSV
TPQTSRSGHVGLPVPGNEAKLVPNNGKLEVRARGPNITPGYWRAPELTAKAFDEEGFYKLNDALKPVDPNDLSAGFDFDG
RISEDFKLASGTWVSVGPLRAKLIAACAPLVRDVVIAGLDRDTVSALAILDADGCKLINPTLQLDDLAGMAADDLIRDAF
RERLGRLLSQATGSSNRITRAVLLDEPLSIDKGEVTDKGSVNQRAVLEHRAALIADLYAEPPPAHVIAVG

Sequences:

>Translated_630_residues
MGAMTNPTARAGDVEHGEPHPLRDISFGDIGIATERRPDGTIYVRSTTTLTDYPVRITDRLHHFAETAPDRVFMAERDGS
GGWRKITYAGMLGAARKIASALLARGLSAERPVMILSGNSIDHAQMMFGALYAGVAMCPVSPPYSLVSKDFGKLRYIVGL
LTPGLIFADDVTPFAPAILATVPSDVEIAATRGAVEGRRVTSLDELLNTPEHPGLATAHDAIGHDTIAKFLLTSGSTGNP
KAVINTQRMICANQVMIREAMAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYIDDGKPTPAGIAATIRNLREIA
PTVYFNVPKGYESLLPVLREDAQLRKMFFSRLHAMFFSGASLAAHVWKGLDEVGAQETGARVPMLTGLGATETAPFFMSV
TPQTSRSGHVGLPVPGNEAKLVPNNGKLEVRARGPNITPGYWRAPELTAKAFDEEGFYKLNDALKPVDPNDLSAGFDFDG
RISEDFKLASGTWVSVGPLRAKLIAACAPLVRDVVIAGLDRDTVSALAILDADGCKLINPTLQLDDLAGMAADDLIRDAF
RERLGRLLSQATGSSNRITRAVLLDEPLSIDKGEVTDKGSVNQRAVLEHRAALIADLYAEPPPAHVIAVG
>Mature_629_residues
GAMTNPTARAGDVEHGEPHPLRDISFGDIGIATERRPDGTIYVRSTTTLTDYPVRITDRLHHFAETAPDRVFMAERDGSG
GWRKITYAGMLGAARKIASALLARGLSAERPVMILSGNSIDHAQMMFGALYAGVAMCPVSPPYSLVSKDFGKLRYIVGLL
TPGLIFADDVTPFAPAILATVPSDVEIAATRGAVEGRRVTSLDELLNTPEHPGLATAHDAIGHDTIAKFLLTSGSTGNPK
AVINTQRMICANQVMIREAMAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYIDDGKPTPAGIAATIRNLREIAP
TVYFNVPKGYESLLPVLREDAQLRKMFFSRLHAMFFSGASLAAHVWKGLDEVGAQETGARVPMLTGLGATETAPFFMSVT
PQTSRSGHVGLPVPGNEAKLVPNNGKLEVRARGPNITPGYWRAPELTAKAFDEEGFYKLNDALKPVDPNDLSAGFDFDGR
ISEDFKLASGTWVSVGPLRAKLIAACAPLVRDVVIAGLDRDTVSALAILDADGCKLINPTLQLDDLAGMAADDLIRDAFR
ERLGRLLSQATGSSNRITRAVLLDEPLSIDKGEVTDKGSVNQRAVLEHRAALIADLYAEPPPAHVIAVG

Specific function: Catalyzes the ligation of 3-hydroxybenzoate or 4- hydroxybenzoate and CoA at the expense of ATP. The enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4- chlorobenzoate.

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=413, Percent_Identity=24.455205811138, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI145693145, Length=431, Percent_Identity=23.8979118329466, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17564090, Length=445, Percent_Identity=25.6179775280899, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556552, Length=332, Percent_Identity=27.1084337349398, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.27 [H]

Molecular weight: Translated: 67608; Mature: 67477

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAMTNPTARAGDVEHGEPHPLRDISFGDIGIATERRPDGTIYVRSTTTLTDYPVRITDR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECEECCCCCHHHHH
LHHFAETAPDRVFMAERDGSGGWRKITYAGMLGAARKIASALLARGLSAERPVMILSGNS
HHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
IDHAQMMFGALYAGVAMCPVSPPYSLVSKDFGKLRYIVGLLTPGLIFADDVTPFAPAILA
CCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
TVPSDVEIAATRGAVEGRRVTSLDELLNTPEHPGLATAHDAIGHDTIAKFLLTSGSTGNP
CCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCC
KAVINTQRMICANQVMIREAMAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYID
CEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEE
DGKPTPAGIAATIRNLREIAPTVYFNVPKGYESLLPVLREDAQLRKMFFSRLHAMFFSGA
CCCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLAAHVWKGLDEVGAQETGARVPMLTGLGATETAPFFMSVTPQTSRSGHVGLPVPGNEAK
HHHHHHHHCHHHHCCHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCCCCE
LVPNNGKLEVRARGPNITPGYWRAPELTAKAFDEEGFYKLNDALKPVDPNDLSAGFDFDG
EECCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
RISEDFKLASGTWVSVGPLRAKLIAACAPLVRDVVIAGLDRDTVSALAILDADGCKLINP
CCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECC
TLQLDDLAGMAADDLIRDAFRERLGRLLSQATGSSNRITRAVLLDEPLSIDKGEVTDKGS
CEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
VNQRAVLEHRAALIADLYAEPPPAHVIAVG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure 
GAMTNPTARAGDVEHGEPHPLRDISFGDIGIATERRPDGTIYVRSTTTLTDYPVRITDR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECEECCCCCHHHHH
LHHFAETAPDRVFMAERDGSGGWRKITYAGMLGAARKIASALLARGLSAERPVMILSGNS
HHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
IDHAQMMFGALYAGVAMCPVSPPYSLVSKDFGKLRYIVGLLTPGLIFADDVTPFAPAILA
CCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
TVPSDVEIAATRGAVEGRRVTSLDELLNTPEHPGLATAHDAIGHDTIAKFLLTSGSTGNP
CCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCC
KAVINTQRMICANQVMIREAMAFLKDEPPVIVDWLPWNHTFGGNHNIGLTLFNGGSMYID
CEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEE
DGKPTPAGIAATIRNLREIAPTVYFNVPKGYESLLPVLREDAQLRKMFFSRLHAMFFSGA
CCCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLAAHVWKGLDEVGAQETGARVPMLTGLGATETAPFFMSVTPQTSRSGHVGLPVPGNEAK
HHHHHHHHCHHHHCCHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCCCCE
LVPNNGKLEVRARGPNITPGYWRAPELTAKAFDEEGFYKLNDALKPVDPNDLSAGFDFDG
EECCCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
RISEDFKLASGTWVSVGPLRAKLIAACAPLVRDVVIAGLDRDTVSALAILDADGCKLINP
CCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECC
TLQLDDLAGMAADDLIRDAFRERLGRLLSQATGSSNRITRAVLLDEPLSIDKGEVTDKGS
CEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
VNQRAVLEHRAALIADLYAEPPPAHVIAVG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11208796 [H]