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The map label for this gene is paaF [C]

Identifier: 86750688

GI number: 86750688

Start: 4102740

End: 4103546

Strand: Reverse

Name: paaF [C]

Synonym: RPB_3578

Alternate gene names: 86750688

Gene position: 4103546-4102740 (Counterclockwise)

Preceding gene: 86750689

Following gene: 86750687

Centisome position: 76.97

GC content: 65.43

Gene sequence:

>807_bases
GTGCTCACAGGAAACGCCTCCGCCGCGACGCTCGCGGACCCCTCGACTCTGGTGATCGACACCGTCGGCTCGGTGCTGAC
GGTCGGCCTCAACCGTGCCAAGAAACGCAATGCGCTGAACGACGGCCTGATGGCCGCGCTGAAGGATTGCTTCGACGATC
TGCCGGCCGACATCCGCTCGGTGGTGATCCACGGCATCGGCGATCACTTCTCCGCCGGCCTCGATCTGTCGGAGCTGCGC
GTGCGCGACGCCACCGAAGGGCTGGTGCATTCGCAGACCTGGCACCGGGTGTTCGACAAGATTCAATATTGCCGCGTGCC
GGTGATCGCGGCGCTGAAGGGCGCGGTGATCGGCGGCGGGCTCGAACTGGCCTGCGCCGCGCATATCCGCGTCGCCGAGC
CGTCGGCCTACTACGCACTGCCGGAAGGCAGCCGCGGCATTTTCGTCGGCGGCGGCGGATCGGTGCGCCTGCCGCGGCTG
ATCGGCGTGGCGCGGATGGCCGACATGATGCTGACCGGCCGGGTGTATTCGGCAACCGAAGGCGTCGTGCACGGTTTCTC
GCAATATCTGTCGGAAGACAAAACCGCCTACGACAAGGCGCTCGAACTCGGCAATCGCGTCGCCCAGAATGCGCCGCTGA
CCAATTTCGCGGTGCTGCAGGCGCTGCCGATGATCGCCGAGGCCAATCCGCAGACCGGTCTGCTGATGGAATCGCTGATG
GCGACGGTGGCGCAGAGCGACCAAGAGGCCAAGAACCGGATTCGCGACTTCCTCGATCACAAGACCGCAAAGGTTAAACC
GACCTGA

Upstream 100 bases:

>100_bases
CGTGCTGCGATCGACAACGGGCTCGAAATGTCCGCGCACACCGCATTGACTCCCGCCAAATTCGTTATAGTCTATAACTA
TTCTGCACAGGACGTAATAA

Downstream 100 bases:

>100_bases
GATGGGCGCCATGACCAACCCGACTGCGCGCGCCGGCGACGTGGAGCACGGCGAGCCTCATCCGCTACGCGACATTTCGT
TCGGCGACATCGGGATCGCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLTGNASAATLADPSTLVIDTVGSVLTVGLNRAKKRNALNDGLMAALKDCFDDLPADIRSVVIHGIGDHFSAGLDLSELR
VRDATEGLVHSQTWHRVFDKIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYYALPEGSRGIFVGGGGSVRLPRL
IGVARMADMMLTGRVYSATEGVVHGFSQYLSEDKTAYDKALELGNRVAQNAPLTNFAVLQALPMIAEANPQTGLLMESLM
ATVAQSDQEAKNRIRDFLDHKTAKVKPT

Sequences:

>Translated_268_residues
MLTGNASAATLADPSTLVIDTVGSVLTVGLNRAKKRNALNDGLMAALKDCFDDLPADIRSVVIHGIGDHFSAGLDLSELR
VRDATEGLVHSQTWHRVFDKIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYYALPEGSRGIFVGGGGSVRLPRL
IGVARMADMMLTGRVYSATEGVVHGFSQYLSEDKTAYDKALELGNRVAQNAPLTNFAVLQALPMIAEANPQTGLLMESLM
ATVAQSDQEAKNRIRDFLDHKTAKVKPT
>Mature_268_residues
MLTGNASAATLADPSTLVIDTVGSVLTVGLNRAKKRNALNDGLMAALKDCFDDLPADIRSVVIHGIGDHFSAGLDLSELR
VRDATEGLVHSQTWHRVFDKIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYYALPEGSRGIFVGGGGSVRLPRL
IGVARMADMMLTGRVYSATEGVVHGFSQYLSEDKTAYDKALELGNRVAQNAPLTNFAVLQALPMIAEANPQTGLLMESLM
ATVAQSDQEAKNRIRDFLDHKTAKVKPT

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.9610894941634, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI194097323, Length=266, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI68989263, Length=194, Percent_Identity=32.4742268041237, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502327, Length=247, Percent_Identity=32.3886639676113, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=32.7683615819209, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI31542718, Length=202, Percent_Identity=26.2376237623762, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=32.421875, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI221142681, Length=197, Percent_Identity=34.010152284264, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788682, Length=154, Percent_Identity=34.4155844155844, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=29.3785310734463, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787660, Length=191, Percent_Identity=30.3664921465969, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=204, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=192, Percent_Identity=30.2083333333333, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=31.4285714285714, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=202, Percent_Identity=33.1683168316832, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=147, Percent_Identity=38.7755102040816, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=147, Percent_Identity=38.7755102040816, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=147, Percent_Identity=38.7755102040816, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=156, Percent_Identity=31.4102564102564, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=229, Percent_Identity=28.3842794759825, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=220, Percent_Identity=30.9090909090909, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=29.2035398230088, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357171, Length=205, Percent_Identity=29.7560975609756, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=28.2178217821782, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=160, Percent_Identity=35.625, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583079, Length=160, Percent_Identity=35.625, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921000, Length=160, Percent_Identity=35.625, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28554; Mature: 28554

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGNASAATLADPSTLVIDTVGSVLTVGLNRAKKRNALNDGLMAALKDCFDDLPADIRS
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVIHGIGDHFSAGLDLSELRVRDATEGLVHSQTWHRVFDKIQYCRVPVIAALKGAVIGGG
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCC
LELACAAHIRVAEPSAYYALPEGSRGIFVGGGGSVRLPRLIGVARMADMMLTGRVYSATE
HHEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHCCHHHHHH
GVVHGFSQYLSEDKTAYDKALELGNRVAQNAPLTNFAVLQALPMIAEANPQTGLLMESLM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
ATVAQSDQEAKNRIRDFLDHKTAKVKPT
HHHHCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLTGNASAATLADPSTLVIDTVGSVLTVGLNRAKKRNALNDGLMAALKDCFDDLPADIRS
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVIHGIGDHFSAGLDLSELRVRDATEGLVHSQTWHRVFDKIQYCRVPVIAALKGAVIGGG
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCC
LELACAAHIRVAEPSAYYALPEGSRGIFVGGGGSVRLPRLIGVARMADMMLTGRVYSATE
HHEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHCCHHHHHH
GVVHGFSQYLSEDKTAYDKALELGNRVAQNAPLTNFAVLQALPMIAEANPQTGLLMESLM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
ATVAQSDQEAKNRIRDFLDHKTAKVKPT
HHHHCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA