BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is echA8 [H]

Identifier: 86750667

GI number: 86750667

Start: 4070564

End: 4071343

Strand: Direct

Name: echA8 [H]

Synonym: RPB_3557

Alternate gene names: 86750667

Gene position: 4070564-4071343 (Clockwise)

Preceding gene: 86750666

Following gene: 86750668

Centisome position: 76.35

GC content: 63.33

Gene sequence:

>780_bases
ATGAGCACTTTTGAATTCATCATCGTCGAACGCCAGAACGCCGTCGGCATCATCAAGCTCAACCGTCCGAAGCTGTTGAA
CGCGCTGTCGTTCGGTGTGTTCGGCGAGATCGGCGCGGCGGTCGATGATCTCGAAGCCGACGACGCGATCGGATGCATCC
TGATCACCGGCAGTGACAAGGCGTTCGCCGCCGGCGCCGATATCAAGGAGATGCAGCCGAAGGGCTTCATCGACATGTTC
AACGAGGACTTCACCTCGATCGGCGGCGACCGTCTGGCGCGCTGCCGCAAGCCGACCATCGCCGCGGTCGCCGGCTACGC
GCTCGGCGGCGGCTGCGAATTGGCGATGATGTGCGACATCATCATCGCGGCCGACACTGCGAAGTTCGGCCAGCCGGAAA
TCACCCTCGGAACAATCCCGGGCATTGGCGGGACGCAGCGCCTGACGCGCGCCATCGGCAAGTCGAAGGCGATGGATCTG
TGCCTCACCGGACGGATGATGGATGCGCAGGAAGCCGAACGCGCCGGCCTGGTCAGCAGGATCGTGCCCGCCGACAAGCT
GATGGAAGAGGCGCTCGCCGCCGCCGAGAAGATCGCGTCGATGTCGTATCCTGCGGCCGCGATGGCGAAAAGCGCGGTGA
ACCGTGCGCTGGAAACCACACTGGCCGAAGGTCTCGCGGTCGAGCGCGATTTGTTCCGCTCCAGCTTCGCACTCGAAGAT
CGCTCCGAGGGCATGGCGGCCTTCATCGAAAAGCGCAAACCGAACAACAAGAACCGCTGA

Upstream 100 bases:

>100_bases
CGCGCGGATGTTCATGGCCTGACCCGCCTCCCCTTGAAGCCGGAGCCGCCAGCGGGCAGATTGCCCGCGGCCGCGACCGG
CCCTCGAGCGGGAATGCATC

Downstream 100 bases:

>100_bases
CAGCCGGCTGACAGGCAAGGGCCGGGGCGATTTGACGCCGCATCATGCCACGCGCCTGATCGTCGCAGCGTTGCTCCATG
ACCACAGTCGCGGCGACAAC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSTFEFIIVERQNAVGIIKLNRPKLLNALSFGVFGEIGAAVDDLEADDAIGCILITGSDKAFAAGADIKEMQPKGFIDMF
NEDFTSIGGDRLARCRKPTIAAVAGYALGGGCELAMMCDIIIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDL
CLTGRMMDAQEAERAGLVSRIVPADKLMEEALAAAEKIASMSYPAAAMAKSAVNRALETTLAEGLAVERDLFRSSFALED
RSEGMAAFIEKRKPNNKNR

Sequences:

>Translated_259_residues
MSTFEFIIVERQNAVGIIKLNRPKLLNALSFGVFGEIGAAVDDLEADDAIGCILITGSDKAFAAGADIKEMQPKGFIDMF
NEDFTSIGGDRLARCRKPTIAAVAGYALGGGCELAMMCDIIIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDL
CLTGRMMDAQEAERAGLVSRIVPADKLMEEALAAAEKIASMSYPAAAMAKSAVNRALETTLAEGLAVERDLFRSSFALED
RSEGMAAFIEKRKPNNKNR
>Mature_258_residues
STFEFIIVERQNAVGIIKLNRPKLLNALSFGVFGEIGAAVDDLEADDAIGCILITGSDKAFAAGADIKEMQPKGFIDMFN
EDFTSIGGDRLARCRKPTIAAVAGYALGGGCELAMMCDIIIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDLC
LTGRMMDAQEAERAGLVSRIVPADKLMEEALAAAEKIASMSYPAAAMAKSAVNRALETTLAEGLAVERDLFRSSFALEDR
SEGMAAFIEKRKPNNKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=57.4712643678161, Blast_Score=291, Evalue=5e-79,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=31.6872427983539, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=37.6470588235294, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=36.5079365079365, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=31.6981132075472, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=35.3932584269663, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI37594469, Length=246, Percent_Identity=30.0813008130081, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI37594471, Length=246, Percent_Identity=30.0813008130081, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=32.0987654320988, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI157694516, Length=233, Percent_Identity=26.6094420600858, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=233, Percent_Identity=26.6094420600858, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI25453481, Length=269, Percent_Identity=23.7918215613383, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538573, Length=269, Percent_Identity=23.7918215613383, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI261878539, Length=93, Percent_Identity=43.010752688172, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=284, Percent_Identity=24.2957746478873, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI51538581, Length=284, Percent_Identity=24.2957746478873, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4757968, Length=268, Percent_Identity=22.7611940298507, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI49355787, Length=268, Percent_Identity=22.7611940298507, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI62530384, Length=248, Percent_Identity=24.5967741935484, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=260, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221136753, Length=260, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221307494, Length=260, Percent_Identity=23.0769230769231, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI157694520, Length=193, Percent_Identity=25.9067357512953, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=46.484375, Blast_Score=214, Evalue=3e-57,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=35.0194552529183, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI221142681, Length=273, Percent_Identity=33.6996336996337, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=34.7490347490347, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=31.1787072243346, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788682, Length=217, Percent_Identity=32.258064516129, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=55.6, Blast_Score=265, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=50.4166666666667, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=32.3144104803493, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17540306, Length=254, Percent_Identity=31.496062992126, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=265, Percent_Identity=33.2075471698113, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=227, Percent_Identity=33.0396475770925, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=212, Percent_Identity=34.4339622641509, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=212, Percent_Identity=34.4339622641509, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=212, Percent_Identity=34.4339622641509, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=275, Percent_Identity=30.5454545454545, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=32.0388349514563, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=28.125, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=25.0980392156863, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=27.1356783919598, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=55.9055118110236, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=55.9055118110236, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=35.8490566037736, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=28.4615384615385, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21357171, Length=264, Percent_Identity=30.6818181818182, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=33.7349397590361, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=32.1951219512195, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24654903, Length=185, Percent_Identity=29.1891891891892, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19921000, Length=223, Percent_Identity=35.8744394618834, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583077, Length=223, Percent_Identity=35.8744394618834, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583079, Length=223, Percent_Identity=35.8744394618834, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571729, Length=186, Percent_Identity=31.1827956989247, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28571730, Length=186, Percent_Identity=31.1827956989247, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24583165, Length=187, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27547; Mature: 27416

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFEFIIVERQNAVGIIKLNRPKLLNALSFGVFGEIGAAVDDLEADDAIGCILITGSDK
CCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
AFAAGADIKEMQPKGFIDMFNEDFTSIGGDRLARCRKPTIAAVAGYALGGGCELAMMCDI
CEECCCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
IIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDLCLTGRMMDAQEAERAGLVSR
HEEECCCCCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHH
IVPADKLMEEALAAAEKIASMSYPAAAMAKSAVNRALETTLAEGLAVERDLFRSSFALED
HCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RSEGMAAFIEKRKPNNKNR
HHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STFEFIIVERQNAVGIIKLNRPKLLNALSFGVFGEIGAAVDDLEADDAIGCILITGSDK
CCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
AFAAGADIKEMQPKGFIDMFNEDFTSIGGDRLARCRKPTIAAVAGYALGGGCELAMMCDI
CEECCCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
IIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDLCLTGRMMDAQEAERAGLVSR
HEEECCCCCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHH
IVPADKLMEEALAAAEKIASMSYPAAAMAKSAVNRALETTLAEGLAVERDLFRSSFALED
HCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RSEGMAAFIEKRKPNNKNR
HHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]