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The map label for this gene is mdtE [H]

Identifier: 86750522

GI number: 86750522

Start: 3898432

End: 3899523

Strand: Reverse

Name: mdtE [H]

Synonym: RPB_3411

Alternate gene names: 86750522

Gene position: 3899523-3898432 (Counterclockwise)

Preceding gene: 86750525

Following gene: 86750521

Centisome position: 73.14

GC content: 66.85

Gene sequence:

>1092_bases
ATGAGCCAACCCACCATGTTACCGCCGCTTCGGATCATCCTGTTGCTGCTTATGGCCACGCCGCTGGCGGCGTGCGGCGA
GAAGGCTCAGCAAGCCCGGCCGATCAATCTGGTGAAGACCGAGATCGTGCACCTCGAGCCCCGGCAGACGATTGTTCGTC
TCACCGGCGACGTCCAGGCGCGCGTGACCAGCGAACTGTCCTTCCGCGTCAGTGGCCGGGTCACCGAGCGGCTGGTCGAT
GTCGGCGCCCATGTGAATGCCGGCGACGTGCTGGCGCGCATCGATCCGACCGAGCAACAGGCCGACCTGGTCGGCTCGCA
GGCCGCCGTCGCCTCGGCGGAGGCGCAACTGCGCCTCGCCAATGCGACCTTCGACCGGCAGAAATCGCTGATGGCCAGCG
GCTTCACCACCCGCAGCTCCTACGACCAGGCGCAGGAGGGATTGCGGACCGCCGAAGGCTCCCTCGACAACGCCAAGGCC
CAGCTCGAAATCGCAAGGGATGCGCTGACCTATACCGAACTGCGCGCGAGCGCGTCGGGCATCATCACGGCGCGCAACAT
CGAGGTCGGGCAGGTCGCCCAATCCGCCCAGTCGGCCTACACGCTGGCGGAGGATGGCGCCCGCGACGCCGTGTTCGACG
TCTACGAATCGGTGTTTCTGACGCCGCTCCAGGGCGGCACCATCAAACTCACGCTGGTGTCGGATCCGTCGGTCACCGCC
ATCGGGCGTCCGCGGGAGATTTCTCCCACCGTCGACCAGAAGAGCGGCACCGTTCGGGTCAAGCTGTCGATCGAGAATCC
GCCGGCCGCGATGACGCTCGGCAGCGCCGTCACGGGCGAGGGGCGCAGCCGTTCCGTGGACAAGATCGTGCTGCCCTGGA
GTGCATTGACCTCCGACCAGAAAGGGCCGGCCGTCTGGGTGATCGACCCCAAGACCCGCGCCGTGTCGCTCAGGAGCGTC
ACCGTGGAAAGCTACGAAACCAGCTCGATCATCGTGGCCGACGGCCTGAAGGCCGGCGAGCGCGTCGTCGTCGACGGCGG
CAAGATGCTCCGCCCCGCGGAGATCGTCACTTATGACGGGGAAAACGCATGA

Upstream 100 bases:

>100_bases
TTCTTGGCTTCGGGAGGCATTGCAGGAACTCCAATCGTTCGCAGCCTACTATTGACTTTCGAGTCAGTCAATATTATTGC
AGAGCAACACGGTCGATCAA

Downstream 100 bases:

>100_bases
CGGCGTCGCGGGTCACAGGGGTGATTGCAGCCTGCCTGCTCATCGCGGGCTGTCAGGAAAAAGCCAGCCAGCCGGAACCG
ATCCGGCCGGTGCTGTCGAC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSQPTMLPPLRIILLLLMATPLAACGEKAQQARPINLVKTEIVHLEPRQTIVRLTGDVQARVTSELSFRVSGRVTERLVD
VGAHVNAGDVLARIDPTEQQADLVGSQAAVASAEAQLRLANATFDRQKSLMASGFTTRSSYDQAQEGLRTAEGSLDNAKA
QLEIARDALTYTELRASASGIITARNIEVGQVAQSAQSAYTLAEDGARDAVFDVYESVFLTPLQGGTIKLTLVSDPSVTA
IGRPREISPTVDQKSGTVRVKLSIENPPAAMTLGSAVTGEGRSRSVDKIVLPWSALTSDQKGPAVWVIDPKTRAVSLRSV
TVESYETSSIIVADGLKAGERVVVDGGKMLRPAEIVTYDGENA

Sequences:

>Translated_363_residues
MSQPTMLPPLRIILLLLMATPLAACGEKAQQARPINLVKTEIVHLEPRQTIVRLTGDVQARVTSELSFRVSGRVTERLVD
VGAHVNAGDVLARIDPTEQQADLVGSQAAVASAEAQLRLANATFDRQKSLMASGFTTRSSYDQAQEGLRTAEGSLDNAKA
QLEIARDALTYTELRASASGIITARNIEVGQVAQSAQSAYTLAEDGARDAVFDVYESVFLTPLQGGTIKLTLVSDPSVTA
IGRPREISPTVDQKSGTVRVKLSIENPPAAMTLGSAVTGEGRSRSVDKIVLPWSALTSDQKGPAVWVIDPKTRAVSLRSV
TVESYETSSIIVADGLKAGERVVVDGGKMLRPAEIVTYDGENA
>Mature_362_residues
SQPTMLPPLRIILLLLMATPLAACGEKAQQARPINLVKTEIVHLEPRQTIVRLTGDVQARVTSELSFRVSGRVTERLVDV
GAHVNAGDVLARIDPTEQQADLVGSQAAVASAEAQLRLANATFDRQKSLMASGFTTRSSYDQAQEGLRTAEGSLDNAKAQ
LEIARDALTYTELRASASGIITARNIEVGQVAQSAQSAYTLAEDGARDAVFDVYESVFLTPLQGGTIKLTLVSDPSVTAI
GRPREISPTVDQKSGTVRVKLSIENPPAAMTLGSAVTGEGRSRSVDKIVLPWSALTSDQKGPAVWVIDPKTRAVSLRSVT
VESYETSSIIVADGLKAGERVVVDGGKMLRPAEIVTYDGENA

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=28.8, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=29.3333333333333, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789665, Length=365, Percent_Identity=26.3013698630137, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082036, Length=300, Percent_Identity=25, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081951, Length=181, Percent_Identity=31.4917127071823, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081796, Length=340, Percent_Identity=25.5882352941176, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38647; Mature: 38516

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPTMLPPLRIILLLLMATPLAACGEKAQQARPINLVKTEIVHLEPRQTIVRLTGDVQA
CCCCCCCCHHHHHHHHHHHCCHHHHCHHHHHCCCCEEEEEEEEEECCHHEEEEECCCEEE
RVTSELSFRVSGRVTERLVDVGAHVNAGDVLARIDPTEQQADLVGSQAAVASAEAQLRLA
EEEEEEEEEECCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCHHHHHCCCCEEEEE
NATFDRQKSLMASGFTTRSSYDQAQEGLRTAEGSLDNAKAQLEIARDALTYTELRASASG
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHCCCC
IITARNIEVGQVAQSAQSAYTLAEDGARDAVFDVYESVFLTPLQGGTIKLTLVSDPSVTA
EEEEECCCHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHEEEECCCCEEEEEEECCCCEEE
IGRPREISPTVDQKSGTVRVKLSIENPPAAMTLGSAVTGEGRSRSVDKIVLPWSALTSDQ
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCEECCCCCCCCCCEEEECHHHHCCCC
KGPAVWVIDPKTRAVSLRSVTVESYETSSIIVADGLKAGERVVVDGGKMLRPAEIVTYDG
CCCEEEEECCCCEEEEEEEEEEECCCCCEEEEECCCCCCCEEEECCCCEECCEEEEEECC
ENA
CCC
>Mature Secondary Structure 
SQPTMLPPLRIILLLLMATPLAACGEKAQQARPINLVKTEIVHLEPRQTIVRLTGDVQA
CCCCCCCHHHHHHHHHHHCCHHHHCHHHHHCCCCEEEEEEEEEECCHHEEEEECCCEEE
RVTSELSFRVSGRVTERLVDVGAHVNAGDVLARIDPTEQQADLVGSQAAVASAEAQLRLA
EEEEEEEEEECCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCHHHHHCCCCEEEEE
NATFDRQKSLMASGFTTRSSYDQAQEGLRTAEGSLDNAKAQLEIARDALTYTELRASASG
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHCCCC
IITARNIEVGQVAQSAQSAYTLAEDGARDAVFDVYESVFLTPLQGGTIKLTLVSDPSVTA
EEEEECCCHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHEEEECCCCEEEEEEECCCCEEE
IGRPREISPTVDQKSGTVRVKLSIENPPAAMTLGSAVTGEGRSRSVDKIVLPWSALTSDQ
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCEECCCCCCCCCCEEEECHHHHCCCC
KGPAVWVIDPKTRAVSLRSVTVESYETSSIIVADGLKAGERVVVDGGKMLRPAEIVTYDG
CCCEEEEECCCCEEEEEEEEEEECCCCCEEEEECCCCCCCEEEECCCCEECCEEEEEECC
ENA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]