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The map label for this gene is birA [H]

Identifier: 86750410

GI number: 86750410

Start: 3769317

End: 3770180

Strand: Reverse

Name: birA [H]

Synonym: RPB_3299

Alternate gene names: 86750410

Gene position: 3770180-3769317 (Counterclockwise)

Preceding gene: 86750411

Following gene: 86750409

Centisome position: 70.71

GC content: 67.59

Gene sequence:

>864_bases
ATGGTCCGCTTCGCTGACATCATCCCGAACGCAAAGCGCCTCGCTGCGGTCGAGCAGGCCGATGGCATCGGGCGGACCGT
TCTCCACGTCGCCGAGATCGACAGCACCAATCGGGCTCTGATCGATCTCGGCAAGGCCGGCGCCGACCACGGCACCGTCC
TCGTCGCCGATCATCAGACCCGCGGCCGCGGCAAGGGCAGCCGCGCCTGGTTTTCCCAGCCGTCGGGAAGTCTGTGTGTC
TCGACACTGATCCGCACCGCGCGGCCGATCGAGGAGGCGCCGCAATTGGCCCTGCTCGGCGCCGTCGCGCTGTGGGAAGC
GATCGCGGCCCACACCGGCGTTGCCGCCGGCATCAAATGGCCGAACGACCTGTTGGTCGACGGCCGCAAGATCTGCGGCA
TCCTGGCCGAAGCGCACACCGACGGCGCGGGCGAGCTCGACTTCGTCGTGCTCGGCTTTGGCCTGAACGTCGCGATCGCG
CAGGCGGAGTTTCCCGAAGAGCTACGTCGTTCTGCAGCGTCGCTCGCGGCGCTGGCGGGTCGGCCGATCGATCCGATCGG
ACTGCTGAGAGGCTTCCTGGAGTCGCTCGGGGTGTGGCTGCCGCTGTGGGAACGCGACGGTGTCGCGTCGTTCGCCGGCA
CCTGGACCCGGCACGCCGAACATCTCGGCCAAATGGTTCGCGTCACCGACGGTGACGAACCCGTCACGGCACGACTGATC
GGCCTCGCCGACGATGGCGCTCTTCGAATTCTAGACGAGACGGGCGTCACCCGCCTCGTTCACTCCGGCGAAATCGCCAC
GGGCATCTCCGTTGGCCGGGGGCCCGACTACATCTCCACGAAGCACACGGGTGTAAGCTCATGA

Upstream 100 bases:

>100_bases
TGCGGCGTGATCGTCTCCGCCATCGCGGTCGGCATCTTCGCCTCGACGATCTCCCTCGTGGCACCGCACTGAGCCGCATC
GGGAAAGCTGGAGAACGATC

Downstream 100 bases:

>100_bases
AGACCGAACGCAAATGGGACACGCTGGCATCGGATACACGCCAGCGAATGGAGGCCGCGGCGCGCTACATCGGCCCCGGC
AAGCAGGTGAAGAAGGAAGA

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MVRFADIIPNAKRLAAVEQADGIGRTVLHVAEIDSTNRALIDLGKAGADHGTVLVADHQTRGRGKGSRAWFSQPSGSLCV
STLIRTARPIEEAPQLALLGAVALWEAIAAHTGVAAGIKWPNDLLVDGRKICGILAEAHTDGAGELDFVVLGFGLNVAIA
QAEFPEELRRSAASLAALAGRPIDPIGLLRGFLESLGVWLPLWERDGVASFAGTWTRHAEHLGQMVRVTDGDEPVTARLI
GLADDGALRILDETGVTRLVHSGEIATGISVGRGPDYISTKHTGVSS

Sequences:

>Translated_287_residues
MVRFADIIPNAKRLAAVEQADGIGRTVLHVAEIDSTNRALIDLGKAGADHGTVLVADHQTRGRGKGSRAWFSQPSGSLCV
STLIRTARPIEEAPQLALLGAVALWEAIAAHTGVAAGIKWPNDLLVDGRKICGILAEAHTDGAGELDFVVLGFGLNVAIA
QAEFPEELRRSAASLAALAGRPIDPIGLLRGFLESLGVWLPLWERDGVASFAGTWTRHAEHLGQMVRVTDGDEPVTARLI
GLADDGALRILDETGVTRLVHSGEIATGISVGRGPDYISTKHTGVSS
>Mature_287_residues
MVRFADIIPNAKRLAAVEQADGIGRTVLHVAEIDSTNRALIDLGKAGADHGTVLVADHQTRGRGKGSRAWFSQPSGSLCV
STLIRTARPIEEAPQLALLGAVALWEAIAAHTGVAAGIKWPNDLLVDGRKICGILAEAHTDGAGELDFVVLGFGLNVAIA
QAEFPEELRRSAASLAALAGRPIDPIGLLRGFLESLGVWLPLWERDGVASFAGTWTRHAEHLGQMVRVTDGDEPVTARLI
GLADDGALRILDETGVTRLVHSGEIATGISVGRGPDYISTKHTGVSS

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=260, Percent_Identity=25.7692307692308, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790408, Length=258, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30201; Mature: 30201

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRFADIIPNAKRLAAVEQADGIGRTVLHVAEIDSTNRALIDLGKAGADHGTVLVADHQT
CCCCHHHCCCHHHHHHHHHHCCCCHHEEEEECCCCCCCCEEEECCCCCCCCEEEEECCCC
RGRGKGSRAWFSQPSGSLCVSTLIRTARPIEEAPQLALLGAVALWEAIAAHTGVAAGIKW
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PNDLLVDGRKICGILAEAHTDGAGELDFVVLGFGLNVAIAQAEFPEELRRSAASLAALAG
CCHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCC
RPIDPIGLLRGFLESLGVWLPLWERDGVASFAGTWTRHAEHLGQMVRVTDGDEPVTARLI
CCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEE
GLADDGALRILDETGVTRLVHSGEIATGISVGRGPDYISTKHTGVSS
EECCCCCEEEEECCCHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVRFADIIPNAKRLAAVEQADGIGRTVLHVAEIDSTNRALIDLGKAGADHGTVLVADHQT
CCCCHHHCCCHHHHHHHHHHCCCCHHEEEEECCCCCCCCEEEECCCCCCCCEEEEECCCC
RGRGKGSRAWFSQPSGSLCVSTLIRTARPIEEAPQLALLGAVALWEAIAAHTGVAAGIKW
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PNDLLVDGRKICGILAEAHTDGAGELDFVVLGFGLNVAIAQAEFPEELRRSAASLAALAG
CCHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCC
RPIDPIGLLRGFLESLGVWLPLWERDGVASFAGTWTRHAEHLGQMVRVTDGDEPVTARLI
CCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEE
GLADDGALRILDETGVTRLVHSGEIATGISVGRGPDYISTKHTGVSS
EECCCCCEEEEECCCHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]