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The map label for this gene is aceE [H]

Identifier: 86750362

GI number: 86750362

Start: 3716696

End: 3719092

Strand: Reverse

Name: aceE [H]

Synonym: RPB_3251

Alternate gene names: 86750362

Gene position: 3719092-3716696 (Counterclockwise)

Preceding gene: 86750364

Following gene: 86750361

Centisome position: 69.75

GC content: 66.42

Gene sequence:

>2397_bases
ATGCCGCAGCGCCGACTACGTCTCGACATGCTGACTGCGCTGTCGCGCAAGGTGCTGTGGCTGTCGACCTGGACGATCCA
CCACGCCAACCACATCCGTCCCAACGCCGACGGGCTGAAGGTCGGCGGCCATCAGGCCTCGTCGGCGTCGCTCGCGACCA
TCATGTCGGCGCTGTATTTCTCGGTGCTGCGGCCGGAGGACCGCGTCGCGGTCAAGCCGCATGCCAGCCCGATTTTTCAC
GCCATCCAGTATCTGTTCGGGCGGCAGAGCCGCGCCCGGCTGGAGAATTTCCGCGGCTTCAAGGGCGCGCAATCCTATCC
GTCGCGGACCAAGGATGCCGACGACGTCGATTTTTCGACCGGCTCGGTCGGGCTCGGCGTGGCGCAAACGTTGTTCGCCT
CGCTGGTGCAGGACTACGTCAAGTCGCACGGCTGGATGAAGGACCGTCGAGAAGGCCGGATGATCGCGCTGGTCGGCGAT
GCCGAGATGGACGAGGGCAACATCTTCGAGGCGTTGCTCGAAGGCTGGAAGCACGGCCTGCGCAACACCTGGTGGGTGGT
CGACTACAATCGCCAGAGCCTCGACGCGGTGGTGCGAGAGGGGTTGTGGGAGAAATTCGAGTCGATGTTCCGCAATTTCG
GCTGGGACGTCGTCATCGTGAAATACGGCCGGCTGATGCAGCAGGCGTTCGCCGAGCCCGGTGGCGAGGCGCTGCGGCGC
TGGATCGATAACTGCCCCAACCAGATGTACGCGGCGCTGTGCTTTCAGGGCGGCGCGGCGTTCCGCAAGCGGTTGCTCGA
CGAGATCGGCGACCAGGCCGAGGTGTCGCGGCTGATCGATGCGCGCAACGACCACGAGTTGTTGGCGCTGATGTCCAATC
TCGGCGGCCACGATATGGCCAGCATGCTCGAGGCATTCGAACAGATCGATCACGACCGCCCGGTCTGCTTCATCGCCTAC
ACGATCAAGGGCGTCGGCCTGCCGTTCCAGGGCCACAAGGACAATCACGCCGGCCTGATGACGCCGGCGCAGATCGAGAC
CTATCGCACGGCGCAGAACGTCCGGCCCGGCCACGAATGGGATCGGTTCGAGGGGCTGACGCAGGATGCGGCCGCGCTGG
ATGCGTTTCTGAAGACGGCCCCGATCAATACCGGTGGCGAGCGCCGGCTGAGCGCGCCGGTGCTCGAGGTGCCGCAACAA
CTGGCATTCAAGCCCGCGCCGGAGATGTCGACGCAGCAGGGCTTCGGCCTGATCCTCAACGAACTGGCGCGCGGCGAGAC
CGAATTGGCCTCGCGCATCGTCACCGCATCGCCGGACGTCACCGTCTCGACCAATCTCGGCGCCTGGGTCAACCGCCGCG
GCCTGTTCGCCCGCGCCGAAAAGGCGGACCTGTTCCGCAGCGAGAAAATCCCGACCACGTTCAACTGGGACTTTTCGCCG
AAGGGCCAGCACATCGAACTCGGCATCGCCGAGATGAACCTGTTCATCCTGATGTCGGCGCTGGGGCTGTCGCACAGCAT
CAATGGCGAGCGACTGCTGCCGATCGCCACGCTGTACGACCCGTTCATCCAGCGCGGCCTCGATGCGCTGAACTATGCCT
GCTATCAGGATGCCCGTTTCATGGTGGTGGCGACGCCGTCGGGCATCACGCTGGCGCCGGAAGGCGGCGCGCATCAGTCG
ATCGCGACGCCACTGATCGGTATCGCGCAGGACGGGCTGGCGTCGTTCGAGCCGGCCTTTGTCGACGAACTTGCCGTCAT
CATGCGCTGGGGCTTCGAGCACATGCAGCGCGACGACGTCGAGGGCGGCGATTCCGCGGACGGCATGCGCGCCGGCGGCT
CGGTCTACTTGCGGCTGTCGACGCGGACCATCGAGCAGCCGCAGCGGATGATGACCCCCGATTTGCAGAAGTCGATCGTC
GACGGCGCGTATTGGCTGCGTCGACCGGGGCCGAATTGCGAACTGGTGATCGCCACCACCGGCGCGGTCGCGCCCGAAGC
AATCGAAGCGGTCGGCTTGCTCGGCGACAGTCGCCGCGATGTCGGGCTGCTGGCGATCACTTCCGCCGATCGGTTGCACG
CGGGCTGGACCGCCGCGCGCCGGTTGCGCCGCGCGCATCGCGGGTCGCGCCGCGCGAGTCACATCGAGACGCTGCTGGCG
CCGCTGCCGCGCAGTTGCGGCATCGTCACGGTGCTCGACGGTCACCCGGCGACGCTGGGCTGGATCGGGGCGGTCTGCGG
CCATCGCATGGAAGCGCTCGGCGTCGAGCATTTCGGCCAAACCGGCAACATCGCCGACCTGTATCGGCACTACGGCATCG
ACGCCAACGCGATCATCGACGCGGCCGAGAGCCTCATCGCCGGCGCGCCGGTGTTGCACCGGAAGATGGCGGTGTAG

Upstream 100 bases:

>100_bases
GGCAGGATATCCCAATCCGGCCGGCGTTGTCGCCGGGATTTGAGAAGTCCCGGTTTGCGATTCGTCGTAGATTCGGCGGA
CAGAACACCGGGTGATCGCC

Downstream 100 bases:

>100_bases
GTTTCACCGGCGTTGATGGTGAACAAGGGGTTGATTCCACAAATAGTGAACCGGCGCGCCGAATTCGTCCGCGTGACCCG
CATCCGGGGGGCCTCCTTTC

Product: pyruvate dehydrogenase alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 798; Mature: 797

Protein sequence:

>798_residues
MPQRRLRLDMLTALSRKVLWLSTWTIHHANHIRPNADGLKVGGHQASSASLATIMSALYFSVLRPEDRVAVKPHASPIFH
AIQYLFGRQSRARLENFRGFKGAQSYPSRTKDADDVDFSTGSVGLGVAQTLFASLVQDYVKSHGWMKDRREGRMIALVGD
AEMDEGNIFEALLEGWKHGLRNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVVIVKYGRLMQQAFAEPGGEALRR
WIDNCPNQMYAALCFQGGAAFRKRLLDEIGDQAEVSRLIDARNDHELLALMSNLGGHDMASMLEAFEQIDHDRPVCFIAY
TIKGVGLPFQGHKDNHAGLMTPAQIETYRTAQNVRPGHEWDRFEGLTQDAAALDAFLKTAPINTGGERRLSAPVLEVPQQ
LAFKPAPEMSTQQGFGLILNELARGETELASRIVTASPDVTVSTNLGAWVNRRGLFARAEKADLFRSEKIPTTFNWDFSP
KGQHIELGIAEMNLFILMSALGLSHSINGERLLPIATLYDPFIQRGLDALNYACYQDARFMVVATPSGITLAPEGGAHQS
IATPLIGIAQDGLASFEPAFVDELAVIMRWGFEHMQRDDVEGGDSADGMRAGGSVYLRLSTRTIEQPQRMMTPDLQKSIV
DGAYWLRRPGPNCELVIATTGAVAPEAIEAVGLLGDSRRDVGLLAITSADRLHAGWTAARRLRRAHRGSRRASHIETLLA
PLPRSCGIVTVLDGHPATLGWIGAVCGHRMEALGVEHFGQTGNIADLYRHYGIDANAIIDAAESLIAGAPVLHRKMAV

Sequences:

>Translated_798_residues
MPQRRLRLDMLTALSRKVLWLSTWTIHHANHIRPNADGLKVGGHQASSASLATIMSALYFSVLRPEDRVAVKPHASPIFH
AIQYLFGRQSRARLENFRGFKGAQSYPSRTKDADDVDFSTGSVGLGVAQTLFASLVQDYVKSHGWMKDRREGRMIALVGD
AEMDEGNIFEALLEGWKHGLRNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVVIVKYGRLMQQAFAEPGGEALRR
WIDNCPNQMYAALCFQGGAAFRKRLLDEIGDQAEVSRLIDARNDHELLALMSNLGGHDMASMLEAFEQIDHDRPVCFIAY
TIKGVGLPFQGHKDNHAGLMTPAQIETYRTAQNVRPGHEWDRFEGLTQDAAALDAFLKTAPINTGGERRLSAPVLEVPQQ
LAFKPAPEMSTQQGFGLILNELARGETELASRIVTASPDVTVSTNLGAWVNRRGLFARAEKADLFRSEKIPTTFNWDFSP
KGQHIELGIAEMNLFILMSALGLSHSINGERLLPIATLYDPFIQRGLDALNYACYQDARFMVVATPSGITLAPEGGAHQS
IATPLIGIAQDGLASFEPAFVDELAVIMRWGFEHMQRDDVEGGDSADGMRAGGSVYLRLSTRTIEQPQRMMTPDLQKSIV
DGAYWLRRPGPNCELVIATTGAVAPEAIEAVGLLGDSRRDVGLLAITSADRLHAGWTAARRLRRAHRGSRRASHIETLLA
PLPRSCGIVTVLDGHPATLGWIGAVCGHRMEALGVEHFGQTGNIADLYRHYGIDANAIIDAAESLIAGAPVLHRKMAV
>Mature_797_residues
PQRRLRLDMLTALSRKVLWLSTWTIHHANHIRPNADGLKVGGHQASSASLATIMSALYFSVLRPEDRVAVKPHASPIFHA
IQYLFGRQSRARLENFRGFKGAQSYPSRTKDADDVDFSTGSVGLGVAQTLFASLVQDYVKSHGWMKDRREGRMIALVGDA
EMDEGNIFEALLEGWKHGLRNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVVIVKYGRLMQQAFAEPGGEALRRW
IDNCPNQMYAALCFQGGAAFRKRLLDEIGDQAEVSRLIDARNDHELLALMSNLGGHDMASMLEAFEQIDHDRPVCFIAYT
IKGVGLPFQGHKDNHAGLMTPAQIETYRTAQNVRPGHEWDRFEGLTQDAAALDAFLKTAPINTGGERRLSAPVLEVPQQL
AFKPAPEMSTQQGFGLILNELARGETELASRIVTASPDVTVSTNLGAWVNRRGLFARAEKADLFRSEKIPTTFNWDFSPK
GQHIELGIAEMNLFILMSALGLSHSINGERLLPIATLYDPFIQRGLDALNYACYQDARFMVVATPSGITLAPEGGAHQSI
ATPLIGIAQDGLASFEPAFVDELAVIMRWGFEHMQRDDVEGGDSADGMRAGGSVYLRLSTRTIEQPQRMMTPDLQKSIVD
GAYWLRRPGPNCELVIATTGAVAPEAIEAVGLLGDSRRDVGLLAITSADRLHAGWTAARRLRRAHRGSRRASHIETLLAP
LPRSCGIVTVLDGHPATLGWIGAVCGHRMEALGVEHFGQTGNIADLYRHYGIDANAIIDAAESLIAGAPVLHRKMAV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=842, Percent_Identity=23.8717339667458, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 88040; Mature: 87909

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQRRLRLDMLTALSRKVLWLSTWTIHHANHIRPNADGLKVGGHQASSASLATIMSALYF
CCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH
SVLRPEDRVAVKPHASPIFHAIQYLFGRQSRARLENFRGFKGAQSYPSRTKDADDVDFST
HHHCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GSVGLGVAQTLFASLVQDYVKSHGWMKDRREGRMIALVGDAEMDEGNIFEALLEGWKHGL
CCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
RNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVVIVKYGRLMQQAFAEPGGEALRR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHH
WIDNCPNQMYAALCFQGGAAFRKRLLDEIGDQAEVSRLIDARNDHELLALMSNLGGHDMA
HHHHCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
SMLEAFEQIDHDRPVCFIAYTIKGVGLPFQGHKDNHAGLMTPAQIETYRTAQNVRPGHEW
HHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCH
DRFEGLTQDAAALDAFLKTAPINTGGERRLSAPVLEVPQQLAFKPAPEMSTQQGFGLILN
HHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCHHHHHH
ELARGETELASRIVTASPDVTVSTNLGAWVNRRGLFARAEKADLFRSEKIPTTFNWDFSP
HHHCCHHHHHHHHEECCCCEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCC
KGQHIELGIAEMNLFILMSALGLSHSINGERLLPIATLYDPFIQRGLDALNYACYQDARF
CCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVVATPSGITLAPEGGAHQSIATPLIGIAQDGLASFEPAFVDELAVIMRWGFEHMQRDDV
EEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHCCC
EGGDSADGMRAGGSVYLRLSTRTIEQPQRMMTPDLQKSIVDGAYWLRRPGPNCELVIATT
CCCCCCCCCCCCCEEEEEEECCHHHCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
GAVAPEAIEAVGLLGDSRRDVGLLAITSADRLHAGWTAARRLRRAHRGSRRASHIETLLA
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
PLPRSCGIVTVLDGHPATLGWIGAVCGHRMEALGVEHFGQTGNIADLYRHYGIDANAIID
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
AAESLIAGAPVLHRKMAV
HHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
PQRRLRLDMLTALSRKVLWLSTWTIHHANHIRPNADGLKVGGHQASSASLATIMSALYF
CCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH
SVLRPEDRVAVKPHASPIFHAIQYLFGRQSRARLENFRGFKGAQSYPSRTKDADDVDFST
HHHCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GSVGLGVAQTLFASLVQDYVKSHGWMKDRREGRMIALVGDAEMDEGNIFEALLEGWKHGL
CCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
RNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVVIVKYGRLMQQAFAEPGGEALRR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHH
WIDNCPNQMYAALCFQGGAAFRKRLLDEIGDQAEVSRLIDARNDHELLALMSNLGGHDMA
HHHHCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
SMLEAFEQIDHDRPVCFIAYTIKGVGLPFQGHKDNHAGLMTPAQIETYRTAQNVRPGHEW
HHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCH
DRFEGLTQDAAALDAFLKTAPINTGGERRLSAPVLEVPQQLAFKPAPEMSTQQGFGLILN
HHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCHHHHHH
ELARGETELASRIVTASPDVTVSTNLGAWVNRRGLFARAEKADLFRSEKIPTTFNWDFSP
HHHCCHHHHHHHHEECCCCEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCC
KGQHIELGIAEMNLFILMSALGLSHSINGERLLPIATLYDPFIQRGLDALNYACYQDARF
CCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVVATPSGITLAPEGGAHQSIATPLIGIAQDGLASFEPAFVDELAVIMRWGFEHMQRDDV
EEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHCCC
EGGDSADGMRAGGSVYLRLSTRTIEQPQRMMTPDLQKSIVDGAYWLRRPGPNCELVIATT
CCCCCCCCCCCCCEEEEEEECCHHHCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
GAVAPEAIEAVGLLGDSRRDVGLLAITSADRLHAGWTAARRLRRAHRGSRRASHIETLLA
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
PLPRSCGIVTVLDGHPATLGWIGAVCGHRMEALGVEHFGQTGNIADLYRHYGIDANAIID
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
AAESLIAGAPVLHRKMAV
HHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA