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The map label for this gene is ilvD [H]

Identifier: 86750358

GI number: 86750358

Start: 3709231

End: 3710940

Strand: Reverse

Name: ilvD [H]

Synonym: RPB_3247

Alternate gene names: 86750358

Gene position: 3710940-3709231 (Counterclockwise)

Preceding gene: 86750359

Following gene: 86750356

Centisome position: 69.6

GC content: 67.78

Gene sequence:

>1710_bases
ATGGCCGACGGATTGCGCAAGGGGCTGACCAGCTACGGCGACGCCGGTTTTTCGCTGTTCCTGCGCAAGGCGTTCATCAA
GGCGATGGGCTATTCCGACGACGCGCTGGAGCGGCCGATCGTCGGCATCACCAATACCCACAGCGATTACAATCCGTGCC
ACGGCAACGTGCCGCAGATCATCGAGGCGGTGAAGCGCGGCGTGATGCTGGCGGGCGCGATGCCGATGGTGTTTCCGACC
ATCTCGATCGCCGAGAGCTTCGCGCATCCGACCTCGATGTATCTGCGCAATCTGATGGCGATGGACACCGAGGAGATGAT
CCGCGCCCAGCCGATGGATGCGGTGGTAGTGATCGGCGGCTGCGACAAGACGCTGCCGGCGCAGATCATGGCGGCGGTGT
CGGCGGATCTGCCGACGGTGGTGATCCCGGTCGGGCCGATGGTGGTCGGCCATCACAAGGGCGAAGTGCTGGGTGCCTGC
ACCGACTGCCGGCGGTTGTGGGCGAAGCATCGCGCCGGTGAGATCGACGAGGCGGAGATCGAGGCCGTCAACGGCCGGCT
GGCGCCGTCGGTCGGCACCTGCATGGTGATGGGCACCGCCTCGACGATGGCGTGTCTCACCGAGGCGATGGGCCTGTCGC
TGCCGATGAGCGCGACGATCCCGGCGCCGCATGCCGAGCGGTTTCGCTCGGCGGAAGAAAGCGGCAGGGTCGCGGCTGCG
ATGGCCAAGGCGAAAGGCCCGAAGCCGAGCGATCTGCTGACCCCCGCCGCGTTCCGCAACGCGCAAGTCGTGCTGCAGGC
GATCGGCGGCTCGACCAACGGACTGATTCATCTCACCGCGATCGCCGGCCGCGTGCCGCATAAGATCGACCTCGACGGTT
TCGACCGGATCGGCCGCGACGTGCCGGTGCTGGTCGATCTGAAGCCGTCGGGCGATCACTACATGGAGCATTTTCATCAC
GCCGGCGGCGTGCCGAAGCTGATGGCGCAGCTCGGCGAACTGATCGATCTCGACGCGCGGACGATCACCGGCGCGCCGCT
GCGCGACATCGTCGCCAGGGCCGAACACGTGCAGGGCCAGGACGTGATCCGCTCGCGCGACAATCCGATCCGGCGCGAGG
GCGGGCTCGCGATGCTCACCGGCAATCTGGCGCCGCGCGGCGCGGTGATCAAACACGCCGCCGCGTCGCCGCAACTGATG
CAGCACACCGGCCGCGCCGTGGTGTTCGACTCGGTCGAGGACATGACGCTGCGGATCGACGATCCCGATCTCGACGTTGC
GGCCGACGACGTGCTGGTGCTGCGCAATGCCGGGCCGCGCGGCGCGCCGGGGATGCCGGAGGCGGGCTATCTGCCGATCC
CGATGAAGCTGGCGCGGGCGGGCATCAAAGACATGGTGCGCATTTCGGACGCGCGGATGAGCGGCACCGCGTTCGGTACC
ATCGTGCTGCACATCACGCCAGAGAGCGCGGATGGTGGGCCGCTGGCGCTGGTCGAAACCGGCGACCGGATCGCGCTGGA
TGTCGCGGCGCGGCGGATCGATCTGTTGGTTGACGAAAGCGAACTCGCGCGCCGCCGTGCCGCATTGTCGTCGTCAGCCG
CGGCGCGGCCGACGCGCGGCTATGCGCAACTGTTTCACGACACCATCCTGCAGGCCGACGAGGGCTGCGATTTCGATTTT
CTCACCGCAGCCGGGCGCAGCGAGCGTTGA

Upstream 100 bases:

>100_bases
TGCGCCACATCGCCTAGCGTCGCTTCGGCCAGGCCGCCTGCCGATTGACAACGAAATGCGGGAGTTCGTAACTGAGCCGA
CACGAGCACAGGATGACAGA

Downstream 100 bases:

>100_bases
GACGCGCCGCGCTCACATCAAGGTCACTTCCAGTTCTTCAAGCATCGCCAGCGTTTCGAGGTCGCCATTGTCCTCGAGCA
ACTCACGCAGCGTCGGCAGA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALERPIVGITNTHSDYNPCHGNVPQIIEAVKRGVMLAGAMPMVFPT
ISIAESFAHPTSMYLRNLMAMDTEEMIRAQPMDAVVVIGGCDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGAC
TDCRRLWAKHRAGEIDEAEIEAVNGRLAPSVGTCMVMGTASTMACLTEAMGLSLPMSATIPAPHAERFRSAEESGRVAAA
MAKAKGPKPSDLLTPAAFRNAQVVLQAIGGSTNGLIHLTAIAGRVPHKIDLDGFDRIGRDVPVLVDLKPSGDHYMEHFHH
AGGVPKLMAQLGELIDLDARTITGAPLRDIVARAEHVQGQDVIRSRDNPIRREGGLAMLTGNLAPRGAVIKHAAASPQLM
QHTGRAVVFDSVEDMTLRIDDPDLDVAADDVLVLRNAGPRGAPGMPEAGYLPIPMKLARAGIKDMVRISDARMSGTAFGT
IVLHITPESADGGPLALVETGDRIALDVAARRIDLLVDESELARRRAALSSSAAARPTRGYAQLFHDTILQADEGCDFDF
LTAAGRSER

Sequences:

>Translated_569_residues
MADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALERPIVGITNTHSDYNPCHGNVPQIIEAVKRGVMLAGAMPMVFPT
ISIAESFAHPTSMYLRNLMAMDTEEMIRAQPMDAVVVIGGCDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGAC
TDCRRLWAKHRAGEIDEAEIEAVNGRLAPSVGTCMVMGTASTMACLTEAMGLSLPMSATIPAPHAERFRSAEESGRVAAA
MAKAKGPKPSDLLTPAAFRNAQVVLQAIGGSTNGLIHLTAIAGRVPHKIDLDGFDRIGRDVPVLVDLKPSGDHYMEHFHH
AGGVPKLMAQLGELIDLDARTITGAPLRDIVARAEHVQGQDVIRSRDNPIRREGGLAMLTGNLAPRGAVIKHAAASPQLM
QHTGRAVVFDSVEDMTLRIDDPDLDVAADDVLVLRNAGPRGAPGMPEAGYLPIPMKLARAGIKDMVRISDARMSGTAFGT
IVLHITPESADGGPLALVETGDRIALDVAARRIDLLVDESELARRRAALSSSAAARPTRGYAQLFHDTILQADEGCDFDF
LTAAGRSER
>Mature_568_residues
ADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALERPIVGITNTHSDYNPCHGNVPQIIEAVKRGVMLAGAMPMVFPTI
SIAESFAHPTSMYLRNLMAMDTEEMIRAQPMDAVVVIGGCDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGACT
DCRRLWAKHRAGEIDEAEIEAVNGRLAPSVGTCMVMGTASTMACLTEAMGLSLPMSATIPAPHAERFRSAEESGRVAAAM
AKAKGPKPSDLLTPAAFRNAQVVLQAIGGSTNGLIHLTAIAGRVPHKIDLDGFDRIGRDVPVLVDLKPSGDHYMEHFHHA
GGVPKLMAQLGELIDLDARTITGAPLRDIVARAEHVQGQDVIRSRDNPIRREGGLAMLTGNLAPRGAVIKHAAASPQLMQ
HTGRAVVFDSVEDMTLRIDDPDLDVAADDVLVLRNAGPRGAPGMPEAGYLPIPMKLARAGIKDMVRISDARMSGTAFGTI
VLHITPESADGGPLALVETGDRIALDVAARRIDLLVDESELARRRAALSSSAAARPTRGYAQLFHDTILQADEGCDFDFL
TAAGRSER

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=556, Percent_Identity=30.3956834532374, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788157, Length=548, Percent_Identity=31.021897810219, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786464, Length=506, Percent_Identity=31.0276679841897, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI2367371, Length=458, Percent_Identity=32.0960698689956, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322476, Length=554, Percent_Identity=31.0469314079422, Blast_Score=257, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60470; Mature: 60339

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALERPIVGITNTHSDYNPCHGNVPQI
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCCCCCCCCCCCHHHH
IEAVKRGVMLAGAMPMVFPTISIAESFAHPTSMYLRNLMAMDTEEMIRAQPMDAVVVIGG
HHHHHCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEC
CDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGACTDCRRLWAKHRAGEIDEAEI
CCCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
EAVNGRLAPSVGTCMVMGTASTMACLTEAMGLSLPMSATIPAPHAERFRSAEESGRVAAA
HHHCCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHCCCCHHH
MAKAKGPKPSDLLTPAAFRNAQVVLQAIGGSTNGLIHLTAIAGRVPHKIDLDGFDRIGRD
HHHCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEEECCCCCCEECCCCHHHCCCC
VPVLVDLKPSGDHYMEHFHHAGGVPKLMAQLGELIDLDARTITGAPLRDIVARAEHVQGQ
CCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCH
DVIRSRDNPIRREGGLAMLTGNLAPRGAVIKHAAASPQLMQHTGRAVVFDSVEDMTLRID
HHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCHHHHHCCCEEEEECCCCCEEEEC
DPDLDVAADDVLVLRNAGPRGAPGMPEAGYLPIPMKLARAGIKDMVRISDARMSGTAFGT
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IVLHITPESADGGPLALVETGDRIALDVAARRIDLLVDESELARRRAALSSSAAARPTRG
EEEEEECCCCCCCCEEEEECCCEEEEHHHHHHHHEEECHHHHHHHHHHHHCCCCCCCCHH
YAQLFHDTILQADEGCDFDFLTAAGRSER
HHHHHHHHHHHCCCCCCCHHEECCCCCCC
>Mature Secondary Structure 
ADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALERPIVGITNTHSDYNPCHGNVPQI
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCCCCCCCCCCCHHHH
IEAVKRGVMLAGAMPMVFPTISIAESFAHPTSMYLRNLMAMDTEEMIRAQPMDAVVVIGG
HHHHHCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEC
CDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGACTDCRRLWAKHRAGEIDEAEI
CCCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
EAVNGRLAPSVGTCMVMGTASTMACLTEAMGLSLPMSATIPAPHAERFRSAEESGRVAAA
HHHCCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHCCCCHHH
MAKAKGPKPSDLLTPAAFRNAQVVLQAIGGSTNGLIHLTAIAGRVPHKIDLDGFDRIGRD
HHHCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEEECCCCCCEECCCCHHHCCCC
VPVLVDLKPSGDHYMEHFHHAGGVPKLMAQLGELIDLDARTITGAPLRDIVARAEHVQGQ
CCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCH
DVIRSRDNPIRREGGLAMLTGNLAPRGAVIKHAAASPQLMQHTGRAVVFDSVEDMTLRID
HHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCHHHHHCCCEEEEECCCCCEEEEC
DPDLDVAADDVLVLRNAGPRGAPGMPEAGYLPIPMKLARAGIKDMVRISDARMSGTAFGT
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IVLHITPESADGGPLALVETGDRIALDVAARRIDLLVDESELARRRAALSSSAAARPTRG
EEEEEECCCCCCCCEEEEECCCEEEEHHHHHHHHEEECHHHHHHHHHHHHCCCCCCCCHH
YAQLFHDTILQADEGCDFDFLTAAGRSER
HHHHHHHHHHHCCCCCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]