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The map label for this gene is rlmE

Identifier: 86750307

GI number: 86750307

Start: 3654817

End: 3655527

Strand: Reverse

Name: rlmE

Synonym: RPB_3196

Alternate gene names: 86750307

Gene position: 3655527-3654817 (Counterclockwise)

Preceding gene: 86750308

Following gene: 86750305

Centisome position: 68.56

GC content: 66.1

Gene sequence:

>711_bases
ATGGCCAAAGACACCACCGGGCGGATGCGCGTCACCGTCAAGAGCGGCGGCCGGATGAAGCTGTCGTCGAAGCTGTGGCT
CGATCGCCAGCTCAACGACCCCTATGTGGCGCAGGCCAAGCGCGACGGCTATCGCTCGCGCGCGGCCTACAAGCTGACCG
AGATCGACGACAAATATCATTTCCTCAAATCCGGCCAGGCGGTGGTCGATCTCGGCGCCGCGCCCGGAGGCTGGAGCCAG
GTCGCGGCGAAGCGGATCGGCTCGGCGAACGGCCGCGGCAAATTGATCGCGATCGACCTGCTGGAGATGGGCGAGATCCC
CGGCGTCACCTTCGCGCAGCTCGACTTTCTCGACAGTGCCGCGCCGGACAAATTGCGCGAGATGCTCGGCGGCGACGGCG
CCGACGTGGTGATGAGCGACATGGCGGGCAACACCACCGGGCATCGCAAGACCGATCAGCTCCGCATCGTCGGGCTGGTC
GAAAGCGCCGCGCAGTTCGCCAGTGAAGTGCTGAAGCCCGGCGGCATCTTCGTCGCCAAGGTATTTCAGAGCGGCGCCGA
TGCCACGCTGATGAACCAGCTCAAGCGCGACTTCGCCACCGTGAAGCACGTCAAGCCCGCCGCGAGCCGCAAGGATTCCT
CGGAGCGCTACGTGCTGGCGATGGGATTCCGCGGCACACAGCCGGCGGCTCAGGAACCGCAGGAACCGTAG

Upstream 100 bases:

>100_bases
GAAGACGGCGTGCCGGGCGTGGCCTGAGGCGGTTCCGCGCTGGTTTTTCCGCGCTCATCTGCTAAGCAACGCGCCACCAC
CACCAATTTCAAGCAGGGTC

Downstream 100 bases:

>100_bases
TCGCCGGCCAGCTCAGGCAGCGGCGGCCTTGCACCAGCACGCGGCGGCGGTCACAGTCGCTGATCGCGCGCGGTCTGGGT
TTCTTCGGTCGCGGTCTTGG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MAKDTTGRMRVTVKSGGRMKLSSKLWLDRQLNDPYVAQAKRDGYRSRAAYKLTEIDDKYHFLKSGQAVVDLGAAPGGWSQ
VAAKRIGSANGRGKLIAIDLLEMGEIPGVTFAQLDFLDSAAPDKLREMLGGDGADVVMSDMAGNTTGHRKTDQLRIVGLV
ESAAQFASEVLKPGGIFVAKVFQSGADATLMNQLKRDFATVKHVKPAASRKDSSERYVLAMGFRGTQPAAQEPQEP

Sequences:

>Translated_236_residues
MAKDTTGRMRVTVKSGGRMKLSSKLWLDRQLNDPYVAQAKRDGYRSRAAYKLTEIDDKYHFLKSGQAVVDLGAAPGGWSQ
VAAKRIGSANGRGKLIAIDLLEMGEIPGVTFAQLDFLDSAAPDKLREMLGGDGADVVMSDMAGNTTGHRKTDQLRIVGLV
ESAAQFASEVLKPGGIFVAKVFQSGADATLMNQLKRDFATVKHVKPAASRKDSSERYVLAMGFRGTQPAAQEPQEP
>Mature_235_residues
AKDTTGRMRVTVKSGGRMKLSSKLWLDRQLNDPYVAQAKRDGYRSRAAYKLTEIDDKYHFLKSGQAVVDLGAAPGGWSQV
AAKRIGSANGRGKLIAIDLLEMGEIPGVTFAQLDFLDSAAPDKLREMLGGDGADVVMSDMAGNTTGHRKTDQLRIVGLVE
SAAQFASEVLKPGGIFVAKVFQSGADATLMNQLKRDFATVKHVKPAASRKDSSERYVLAMGFRGTQPAAQEPQEP

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=210, Percent_Identity=38.5714285714286, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI29029591, Length=192, Percent_Identity=38.0208333333333, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI29029589, Length=192, Percent_Identity=38.0208333333333, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI7110661, Length=192, Percent_Identity=38.0208333333333, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=35.0785340314136, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=44.7115384615385, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=37.9487179487179, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553474, Length=205, Percent_Identity=36.5853658536585, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71987550, Length=216, Percent_Identity=32.4074074074074, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=216, Percent_Identity=32.4074074074074, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=216, Percent_Identity=32.4074074074074, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=36.3636363636364, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319796, Length=224, Percent_Identity=34.8214285714286, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=123, Percent_Identity=38.2113821138211, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356387, Length=216, Percent_Identity=44.4444444444444, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=36.734693877551, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI18859957, Length=191, Percent_Identity=35.6020942408377, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24647580, Length=209, Percent_Identity=34.4497607655502, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_RHOP2 (Q2IV68)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486803.1
- ProteinModelPortal:   Q2IV68
- SMR:   Q2IV68
- STRING:   Q2IV68
- GeneID:   3910997
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3196
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK819310
- BioCyc:   RPAL316058:RPB_3196-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 25473; Mature: 25342

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 76-76 BINDING 78-78 BINDING 99-99 BINDING 115-115 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDTTGRMRVTVKSGGRMKLSSKLWLDRQLNDPYVAQAKRDGYRSRAAYKLTEIDDKYH
CCCCCCCCEEEEEECCCCEEECCCEEECCCCCCCHHHHHHHCCCHHHHEEEEECCCCHHH
FLKSGQAVVDLGAAPGGWSQVAAKRIGSANGRGKLIAIDLLEMGEIPGVTFAQLDFLDSA
HHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCC
APDKLREMLGGDGADVVMSDMAGNTTGHRKTDQLRIVGLVESAAQFASEVLKPGGIFVAK
CHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEH
VFQSGADATLMNQLKRDFATVKHVKPAASRKDSSERYVLAMGFRGTQPAAQEPQEP
HHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKDTTGRMRVTVKSGGRMKLSSKLWLDRQLNDPYVAQAKRDGYRSRAAYKLTEIDDKYH
CCCCCCCEEEEEECCCCEEECCCEEECCCCCCCHHHHHHHCCCHHHHEEEEECCCCHHH
FLKSGQAVVDLGAAPGGWSQVAAKRIGSANGRGKLIAIDLLEMGEIPGVTFAQLDFLDSA
HHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCC
APDKLREMLGGDGADVVMSDMAGNTTGHRKTDQLRIVGLVESAAQFASEVLKPGGIFVAK
CHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEH
VFQSGADATLMNQLKRDFATVKHVKPAASRKDSSERYVLAMGFRGTQPAAQEPQEP
HHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA