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The map label for this gene is prfC [H]

Identifier: 86750270

GI number: 86750270

Start: 3614910

End: 3616535

Strand: Reverse

Name: prfC [H]

Synonym: RPB_3159

Alternate gene names: 86750270

Gene position: 3616535-3614910 (Counterclockwise)

Preceding gene: 86750272

Following gene: 86750268

Centisome position: 67.83

GC content: 65.62

Gene sequence:

>1626_bases
ATGTCCGACATTGCCATTTCGACCGACGCGCCGTCCCAGCCCCCGTCTTCGCTCGCGCATGAAGTTGCGCGGCGGCGCAC
CTTTGCGATCATCTCGCACCCCGACGCCGGCAAGACCACGCTGACAGAAAAGCTGCTGCTGTTCGGCGGCGCGATCAATC
TCGCCGGGCAGGTCAAGGCCAAGGGCGAGCGCCGCACCACCCGTTCGGACTGGATGAAGATCGAGCGCGACCGCGGCATC
TCGGTGGTCACCTCGGTGATGACCTTCGAGTTCGAGGGACTGGTGTTCAACCTGCTCGACACGCCGGGCCACGAGGACTT
CTCGGAGGACACCTACCGGACGCTGACCGCGGTCGATTCCGCCGTGATGGTGATCGACGCCGCCAAGGGCATCGAGGCGC
GGACGCGCAAGCTGTTCGAGGTCTGCCGGCTGCGCGACATCCCGATCATCACCTTCATCAACAAGATGGACCGCGAGAGC
CGCGACACGTTCGACATCCTGGACGAGATCGAGAAGACGCTGGCGCTCGACACCACGCCGATGACCTGGCCGGTCGGCCG
TGGCCGCGACTTCCTCGGCACCTACGACGTGCTCACCGGCGGCGTGCGTCTCTTGGAAGGCGGCGGCGCCAAGACCGGCG
CGGCCGAGCAGATCGCCATCGAGGATCTCGGCAAGCGCAACCCCAATCTCGATGTCGCTGCGGTGAAGGAAGAGCTGGAA
CTCGCCACCGAAGCCTGCAAGCCGTTCGACCTCGAGGCGTTTCGCGAGGGCCATCTGACGCCGGTGTATTTCGGCTCGGC
GCTGCGCAATTTCGGCGTCGGCGATCTGCTGCAAGGGCTCGGCCGCTACGCGCCGCCGCCGCGCGCGCAGGACTCGAACC
TGCGCAAGGTCGAGGCCGACGAGCCGAAGATGAGCGCCTTCGTCTTCAAGATCCAGGCGAACATGGATCCGAACCACCGC
GATCGCATCGCCTTCGCAAGGCTTTGCTCCGGCAAGCTGACCCGCGGCATGAAGGCCAAGCTGGTCCGCACCGGCAAGAA
CATGAGCCTGTCGGCGCCGCAATTCTTCTTCGCGCAGGATCGCTCGGTGGCGGACGAAGCCTTCGCCGGCGACGTCGTCG
GCATTCCCAATCACGGCACGCTGCGGATCGGCGACACGCTGACCGAGGGCGAGGACGTCACCTTCGTCGGCGTGCCGTCG
TTCGCGCCGGAAATCGTCCGCCGCGTCCGGCTCGGCGACGCGATGAAGGCGAAGAAGCTGAAAGAGGCGCTGCAGCAGAT
GTCCGAAGAGGGCGTCGTGCAGGTGTTCCGCCCGCGCGACGGCGCCCCCGCTCTGGTCGGCGTCGTCGGCCCGCTGCAGC
TCGACGTGCTGAAGGCGCGGCTCGACGCCGAATATCAGCTCCCGGTCGATTTCGAAGTCTCCGAATTCCAGCTCGCGCGC
TGGATCTCGGCCGAGGACCGCAAAAAGCTCGACACCTTCATCACCACCAACAATTCCTCGATCGCCGACGACGTCGACGG
CGACCCGGTGTATCTCGCCAAAAACGAGTTCTATCTCGGCTACACCCGCGACCGCGCCGAAGGCATCGTGTTCTCCGAGA
TCAAGGACGTGAAGAAGGTGGCGTGA

Upstream 100 bases:

>100_bases
ATATGAGGCTGTGAAGGCGCTACAGCAATTCCGTACGCCGCTTGATGTTCGAAGCCTTCGCCCTTGATTGCGCCGGCTTT
CTTTGTCACAGCCTCAGCCC

Downstream 100 bases:

>100_bases
GGCGCTGGGCTCGTGGCCTCCGCTCTGAATACTGGGGCGCCCGTTCGAGCCGGACGATGACCTCTGTCGGCGATGCTCGT
TTATCCGACTGATGGTCCCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSDIAISTDAPSQPPSSLAHEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRTTRSDWMKIERDRGI
SVVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRES
RDTFDILDEIEKTLALDTTPMTWPVGRGRDFLGTYDVLTGGVRLLEGGGAKTGAAEQIAIEDLGKRNPNLDVAAVKEELE
LATEACKPFDLEAFREGHLTPVYFGSALRNFGVGDLLQGLGRYAPPPRAQDSNLRKVEADEPKMSAFVFKIQANMDPNHR
DRIAFARLCSGKLTRGMKAKLVRTGKNMSLSAPQFFFAQDRSVADEAFAGDVVGIPNHGTLRIGDTLTEGEDVTFVGVPS
FAPEIVRRVRLGDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYQLPVDFEVSEFQLAR
WISAEDRKKLDTFITTNNSSIADDVDGDPVYLAKNEFYLGYTRDRAEGIVFSEIKDVKKVA

Sequences:

>Translated_541_residues
MSDIAISTDAPSQPPSSLAHEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRTTRSDWMKIERDRGI
SVVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRES
RDTFDILDEIEKTLALDTTPMTWPVGRGRDFLGTYDVLTGGVRLLEGGGAKTGAAEQIAIEDLGKRNPNLDVAAVKEELE
LATEACKPFDLEAFREGHLTPVYFGSALRNFGVGDLLQGLGRYAPPPRAQDSNLRKVEADEPKMSAFVFKIQANMDPNHR
DRIAFARLCSGKLTRGMKAKLVRTGKNMSLSAPQFFFAQDRSVADEAFAGDVVGIPNHGTLRIGDTLTEGEDVTFVGVPS
FAPEIVRRVRLGDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYQLPVDFEVSEFQLAR
WISAEDRKKLDTFITTNNSSIADDVDGDPVYLAKNEFYLGYTRDRAEGIVFSEIKDVKKVA
>Mature_540_residues
SDIAISTDAPSQPPSSLAHEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRTTRSDWMKIERDRGIS
VVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESR
DTFDILDEIEKTLALDTTPMTWPVGRGRDFLGTYDVLTGGVRLLEGGGAKTGAAEQIAIEDLGKRNPNLDVAAVKEELEL
ATEACKPFDLEAFREGHLTPVYFGSALRNFGVGDLLQGLGRYAPPPRAQDSNLRKVEADEPKMSAFVFKIQANMDPNHRD
RIAFARLCSGKLTRGMKAKLVRTGKNMSLSAPQFFFAQDRSVADEAFAGDVVGIPNHGTLRIGDTLTEGEDVTFVGVPSF
APEIVRRVRLGDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYQLPVDFEVSEFQLARW
ISAEDRKKLDTFITTNNSSIADDVDGDPVYLAKNEFYLGYTRDRAEGIVFSEIKDVKKVA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=476, Percent_Identity=26.0504201680672, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI19923640, Length=501, Percent_Identity=26.1477045908184, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI25306287, Length=295, Percent_Identity=30.5084745762712, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI157426893, Length=153, Percent_Identity=35.9477124183007, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=30.9210526315789, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1790835, Length=528, Percent_Identity=50.1893939393939, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1789738, Length=501, Percent_Identity=25.5489021956088, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=32.0754716981132, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=32.8467153284672, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=503, Percent_Identity=25.4473161033797, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=495, Percent_Identity=24.040404040404, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=35.6060606060606, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=475, Percent_Identity=25.0526315789474, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322359, Length=526, Percent_Identity=25.0950570342205, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=177, Percent_Identity=27.1186440677966, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=31.0126582278481, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=31.0126582278481, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582462, Length=479, Percent_Identity=25.2609603340292, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI221458488, Length=512, Percent_Identity=25.5859375, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI78706572, Length=177, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59683; Mature: 59551

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIAISTDAPSQPPSSLAHEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKA
CCCEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEC
KGERRTTRSDWMKIERDRGISVVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDS
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCEEEEEECCCCCCCCCHHHHHHHHHHCC
AVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRDTFDILDEIEKTLALDTTP
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
MTWPVGRGRDFLGTYDVLTGGVRLLEGGGAKTGAAEQIAIEDLGKRNPNLDVAAVKEELE
CCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
LATEACKPFDLEAFREGHLTPVYFGSALRNFGVGDLLQGLGRYAPPPRAQDSNLRKVEAD
HHHHHCCCCCHHHHHCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEECCC
EPKMSAFVFKIQANMDPNHRDRIAFARLCSGKLTRGMKAKLVRTGKNMSLSAPQFFFAQD
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEECC
RSVADEAFAGDVVGIPNHGTLRIGDTLTEGEDVTFVGVPSFAPEIVRRVRLGDAMKAKKL
CCHHHHHHCCCEEECCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHH
KEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYQLPVDFEVSEFQLAR
HHHHHHHHCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCEECCCCCCHHHHHHHH
WISAEDRKKLDTFITTNNSSIADDVDGDPVYLAKNEFYLGYTRDRAEGIVFSEIKDVKKV
HCCCHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEHHHHHHHHHC
A
C
>Mature Secondary Structure 
SDIAISTDAPSQPPSSLAHEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKA
CCEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEC
KGERRTTRSDWMKIERDRGISVVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDS
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCEEEEEECCCCCCCCCHHHHHHHHHHCC
AVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRDTFDILDEIEKTLALDTTP
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
MTWPVGRGRDFLGTYDVLTGGVRLLEGGGAKTGAAEQIAIEDLGKRNPNLDVAAVKEELE
CCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
LATEACKPFDLEAFREGHLTPVYFGSALRNFGVGDLLQGLGRYAPPPRAQDSNLRKVEAD
HHHHHCCCCCHHHHHCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEECCC
EPKMSAFVFKIQANMDPNHRDRIAFARLCSGKLTRGMKAKLVRTGKNMSLSAPQFFFAQD
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEECC
RSVADEAFAGDVVGIPNHGTLRIGDTLTEGEDVTFVGVPSFAPEIVRRVRLGDAMKAKKL
CCHHHHHHCCCEEECCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHH
KEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYQLPVDFEVSEFQLAR
HHHHHHHHCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCEECCCCCCHHHHHHHH
WISAEDRKKLDTFITTNNSSIADDVDGDPVYLAKNEFYLGYTRDRAEGIVFSEIKDVKKV
HCCCHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA