Click here to switch to the map view.

The map label for this gene is pit [H]

Identifier: 86750272

GI number: 86750272

Start: 3617023

End: 3618033

Strand: Reverse

Name: pit [H]

Synonym: RPB_3161

Alternate gene names: 86750272

Gene position: 3618033-3617023 (Counterclockwise)

Preceding gene: 86750273

Following gene: 86750270

Centisome position: 67.86

GC content: 67.46

Gene sequence:

>1011_bases
GTGGATGCTTCGATCGGACTGCCGATTCTGGTCGGATTGATCGCCGTCGCCCTGCTGTTCGACTTTCTGAACGGCCTGCA
CGACGCCGCCAATTCGATCGCCACCATCGTCTCGACCCGGGTGCTGCGGCCGCAATACGCCGTGTTCTGGGCGGCGTTCT
TCAATTTCATCGCCTTCCTGGTGTTCGGCCTGCACGTCGCCAACACCATCGGCACCGGCATCATCGAGCCGAGCGTGATC
GACGCTTCGGTGATTTTCGCAGCGCTGGTCGGCGCGATCGTCTGGAACGTCGTCACCTGGGGGCTCGGGATTCCGTCGAG
CTCGTCGCACGCGCTGATCGGCGGGCTGCTCGGCGCCGGCCTCGCCAAGGCCGGGCTGTCGGCGGCGCTGTGGAGCGGCT
TGTCCAAGGCGCTGCTGGCGATCGTGCTGTCGCCGCTGGTCGGCTTCCTGCTGGCGCTGGTGCTGGTCGCGATCGTGTCC
TGGGCCTCGGTGCGCTCGACGCCGTTCGCCGTCGACCGCGCCTTCCGCATCCTGCAATTTGTCTCCGCCTCGCTGTATTC
GCTCGGCCATGGCGGCAACGACGCGCAGAAGACGATGGGCATCATCGCGGTGCTTTTGTATTCGCAGGGCTATCTCGGCA
GCGAGTTTTCGGTGCCGTTCTGGGTGGTGATTTCGTGCCAGGCGGCGATGGCGCTCGGCACGTTGATGGGCGGCTGGCGG
ATCGTCCGCACCATGGGCCTGCGGATCACCAAGCTGACGCCGATGCAGGGCTTCTGCGCCGAGACCGGCGGCGCCGCGAC
GCTGTTCATGGCGACCTTCCTCGGCGTGCCGGTGTCGACCACCCACACCATCACCGGCGCGATCGTCGGCGTCGGCGCGG
CGCGGCGGATGTCGGCGGTGCGCTGGAACGTGGCCAGTTCCATCGTCTACGCCTGGGTGATCACGATCCCGGCCGCGGCG
ATCGTCGCGGCGCTGGCGTATTGGGCGACCCGGCTGCTGCCGGCGATGTGA

Upstream 100 bases:

>100_bases
AAATCTACGACCATCTCGAGAAGGTCGCCGATCGGTTCGACGACGTCGCCAACGAGATCAACAGCATCGTCATCGAGCAA
GTCTAGGGCAGGGCCGCATT

Downstream 100 bases:

>100_bases
GCGGGAGCCCGCGCCCGCTTCAGGTCACCACCTTCAGCCCGATGATGCCGGCGACGATCAGGCCGATGCAGGCGAGGCGG
GCCACCGTCGCCGGATCGCC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDASIGLPILVGLIAVALLFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFIAFLVFGLHVANTIGTGIIEPSVI
DASVIFAALVGAIVWNVVTWGLGIPSSSSHALIGGLLGAGLAKAGLSAALWSGLSKALLAIVLSPLVGFLLALVLVAIVS
WASVRSTPFAVDRAFRILQFVSASLYSLGHGGNDAQKTMGIIAVLLYSQGYLGSEFSVPFWVVISCQAAMALGTLMGGWR
IVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGVPVSTTHTITGAIVGVGAARRMSAVRWNVASSIVYAWVITIPAAA
IVAALAYWATRLLPAM

Sequences:

>Translated_336_residues
MDASIGLPILVGLIAVALLFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFIAFLVFGLHVANTIGTGIIEPSVI
DASVIFAALVGAIVWNVVTWGLGIPSSSSHALIGGLLGAGLAKAGLSAALWSGLSKALLAIVLSPLVGFLLALVLVAIVS
WASVRSTPFAVDRAFRILQFVSASLYSLGHGGNDAQKTMGIIAVLLYSQGYLGSEFSVPFWVVISCQAAMALGTLMGGWR
IVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGVPVSTTHTITGAIVGVGAARRMSAVRWNVASSIVYAWVITIPAAA
IVAALAYWATRLLPAM
>Mature_336_residues
MDASIGLPILVGLIAVALLFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFIAFLVFGLHVANTIGTGIIEPSVI
DASVIFAALVGAIVWNVVTWGLGIPSSSSHALIGGLLGAGLAKAGLSAALWSGLSKALLAIVLSPLVGFLLALVLVAIVS
WASVRSTPFAVDRAFRILQFVSASLYSLGHGGNDAQKTMGIIAVLLYSQGYLGSEFSVPFWVVISCQAAMALGTLMGGWR
IVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGVPVSTTHTITGAIVGVGAARRMSAVRWNVASSIVYAWVITIPAAA
IVAALAYWATRLLPAM

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=152, Percent_Identity=34.2105263157895, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=36.1842105263158, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1789360, Length=220, Percent_Identity=32.2727272727273, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789907, Length=216, Percent_Identity=32.8703703703704, Blast_Score=101, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=30.2469135802469, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=165, Percent_Identity=29.6969696969697, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=156, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17536725, Length=172, Percent_Identity=27.3255813953488, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=158, Percent_Identity=30.379746835443, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557328, Length=164, Percent_Identity=30.4878048780488, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=29.746835443038, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=28.8770053475936, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35025; Mature: 35025

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASIGLPILVGLIAVALLFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFIAFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VFGLHVANTIGTGIIEPSVIDASVIFAALVGAIVWNVVTWGLGIPSSSSHALIGGLLGAG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LAKAGLSAALWSGLSKALLAIVLSPLVGFLLALVLVAIVSWASVRSTPFAVDRAFRILQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VSASLYSLGHGGNDAQKTMGIIAVLLYSQGYLGSEFSVPFWVVISCQAAMALGTLMGGWR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
IVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGVPVSTTHTITGAIVGVGAARRMSAV
HHHHHCCEEEEECCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHH
RWNVASSIVYAWVITIPAAAIVAALAYWATRLLPAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDASIGLPILVGLIAVALLFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFIAFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VFGLHVANTIGTGIIEPSVIDASVIFAALVGAIVWNVVTWGLGIPSSSSHALIGGLLGAG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LAKAGLSAALWSGLSKALLAIVLSPLVGFLLALVLVAIVSWASVRSTPFAVDRAFRILQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VSASLYSLGHGGNDAQKTMGIIAVLLYSQGYLGSEFSVPFWVVISCQAAMALGTLMGGWR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
IVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGVPVSTTHTITGAIVGVGAARRMSAV
HHHHHCCEEEEECCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHH
RWNVASSIVYAWVITIPAAAIVAALAYWATRLLPAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]