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The map label for this gene is hsdM [H]

Identifier: 86750172

GI number: 86750172

Start: 3485914

End: 3487461

Strand: Reverse

Name: hsdM [H]

Synonym: RPB_3056

Alternate gene names: 86750172

Gene position: 3487461-3485914 (Counterclockwise)

Preceding gene: 86750173

Following gene: 86750171

Centisome position: 65.41

GC content: 54.59

Gene sequence:

>1548_bases
ATGACGGGGCAAGAACAACGCGCGGCGCTACAACGGAAAATCTGGGATATCGCAAACGATGTTCGCGGCTCCGTTGATGG
CTGGGACTTCAAGCAATATGTCCTAGGTACACTCTTCTATCGGTTCATCAGCGAGAACTTCGCCGCTTATATCGAGGCGG
ATGATGAGAGCATCGATTATGCGGCTCTATCCGATGACGTCATCACCGATGACATCAAGGATGATGCCATCAAGACCAAG
GGCTATTTCATCTATCCCAGCCAGCTGTTCGTGAATGTCGCGAAGAACGCCAACATCAATCACAGCCTGAACACCGACCT
AGCGCACATCTTCGCGGCGATTGAATCATCGGCCAATGGCTACCCTTCCGAGCAGGACATCAGAGGTCTTTTCGCCGATT
TCGACACGACCAGCACGCGTCTTGGCCATACCGTCTCGGAAAAGAACAGCCGATTAGCCAAGGTCCTGAAACGCGTTGCA
GAGCTCGATTTCGGCGACTTCCACAACAGCCAGATTGATTTGTTCGGCGATGCCTACGAATTCCTGATCTCGAACTACGC
CGCCAATGCGGGCAAGTCCGGCGGGGAGTTCTTCACGCCTCAACATGTCTCAAAGCTCATCGCGCAGCTCGCCATGCACG
GGCAAACACAAGTTAACAAGATTTACGACCCGGCCTGCGGCTCCGGCTCGCTACTGTTGCAGGCGAAGAAGCATTTTGAC
GAACATATCATCGAAGAGGGTTTCTTTGGGCAGGAGATCAACCACACGACCTACAACCTCGCCCGCATGAACATGTTCCT
GCACAACATCAACTACGACAAGTTCAATATCCAGCGCGGCGATACGCTGACCCAGCCGCATTTCCAGGACGACAAACCCT
TTGACGCCATCGTCTCCAACCCGCCTTATTCGGTAAAGTGGATCGGCTCAGACGATCCGACCCTAATCAACGATGACCGC
TTTGCTCCGGCGGGGGTGTTGGCACCCAAATCAAAGGCCGATTTCGCCTTTGTGCTCCATGCGCTTAGCTATCTCTCGGC
CAAGGGCCGCGCGGCGATCGTCTGCTTTCCGGGCATTTTCTATCGCGACGGGGCGGAGAAGAAGATCCGACAATATCTGG
TCGATAACAATTACGTTGAGACAGTGATAGCGCTCGCCTCCAACCTCTTCTATGGCACGACCATCGCCGTGACGATCCTT
GTCCTCGCCAAGAACAAGACGGACACGGCCATCCAGTTCATCGACGCCAGCGGCGAAGAGTTTTTCAAGAAGGCCACCAA
CACCAATCTGATGACGGACGATCACATCGCGCGGGTGATGGAGATTTTTGATCGTAAGGAAGACGTCGATCATGTCGCCG
CCTCGGTGCAGTATGAGATCATCGTGGAGCGCGGCTATAACCTCTCGGTCAGCTCCTATGTCGAGCCGCGCGACACGCGC
GAGATTGTCAGTATTGGTGAGCTGAATGCGAAGATCAGAACCACGGTTCTGCGGATCGACCAGCTGCGCGCCGACATCGA
CGCCATCATTGCGGAGATTGACGCATGA

Upstream 100 bases:

>100_bases
AACAGCCCAGGTTCAACGTTGGCGACACCGGGCGGTTTCTGCGTGTGAGTACTAAGGACAACATCAATCGAGTGGCCAGC
GCGAAAAGGGATAAGAAAAC

Downstream 100 bases:

>100_bases
GCGCGGCAGGGTTTCTGGAGGAGCTGCTGGATGGGGCTGATGTGGAGTGGGAACCATTGGGGGAGGTCACTCAACCAACA
GCGAACATCAAATGGTCACA

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTGQEQRAALQRKIWDIANDVRGSVDGWDFKQYVLGTLFYRFISENFAAYIEADDESIDYAALSDDVITDDIKDDAIKTK
GYFIYPSQLFVNVAKNANINHSLNTDLAHIFAAIESSANGYPSEQDIRGLFADFDTTSTRLGHTVSEKNSRLAKVLKRVA
ELDFGDFHNSQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTQVNKIYDPACGSGSLLLQAKKHFD
EHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFNIQRGDTLTQPHFQDDKPFDAIVSNPPYSVKWIGSDDPTLINDDR
FAPAGVLAPKSKADFAFVLHALSYLSAKGRAAIVCFPGIFYRDGAEKKIRQYLVDNNYVETVIALASNLFYGTTIAVTIL
VLAKNKTDTAIQFIDASGEEFFKKATNTNLMTDDHIARVMEIFDRKEDVDHVAASVQYEIIVERGYNLSVSSYVEPRDTR
EIVSIGELNAKIRTTVLRIDQLRADIDAIIAEIDA

Sequences:

>Translated_515_residues
MTGQEQRAALQRKIWDIANDVRGSVDGWDFKQYVLGTLFYRFISENFAAYIEADDESIDYAALSDDVITDDIKDDAIKTK
GYFIYPSQLFVNVAKNANINHSLNTDLAHIFAAIESSANGYPSEQDIRGLFADFDTTSTRLGHTVSEKNSRLAKVLKRVA
ELDFGDFHNSQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTQVNKIYDPACGSGSLLLQAKKHFD
EHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFNIQRGDTLTQPHFQDDKPFDAIVSNPPYSVKWIGSDDPTLINDDR
FAPAGVLAPKSKADFAFVLHALSYLSAKGRAAIVCFPGIFYRDGAEKKIRQYLVDNNYVETVIALASNLFYGTTIAVTIL
VLAKNKTDTAIQFIDASGEEFFKKATNTNLMTDDHIARVMEIFDRKEDVDHVAASVQYEIIVERGYNLSVSSYVEPRDTR
EIVSIGELNAKIRTTVLRIDQLRADIDAIIAEIDA
>Mature_514_residues
TGQEQRAALQRKIWDIANDVRGSVDGWDFKQYVLGTLFYRFISENFAAYIEADDESIDYAALSDDVITDDIKDDAIKTKG
YFIYPSQLFVNVAKNANINHSLNTDLAHIFAAIESSANGYPSEQDIRGLFADFDTTSTRLGHTVSEKNSRLAKVLKRVAE
LDFGDFHNSQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTQVNKIYDPACGSGSLLLQAKKHFDE
HIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFNIQRGDTLTQPHFQDDKPFDAIVSNPPYSVKWIGSDDPTLINDDRF
APAGVLAPKSKADFAFVLHALSYLSAKGRAAIVCFPGIFYRDGAEKKIRQYLVDNNYVETVIALASNLFYGTTIAVTILV
LAKNKTDTAIQFIDASGEEFFKKATNTNLMTDDHIARVMEIFDRKEDVDHVAASVQYEIIVERGYNLSVSSYVEPRDTRE
IVSIGELNAKIRTTVLRIDQLRADIDAIIAEIDA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=284, Percent_Identity=27.4647887323944, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57700; Mature: 57569

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQEQRAALQRKIWDIANDVRGSVDGWDFKQYVLGTLFYRFISENFAAYIEADDESIDY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
AALSDDVITDDIKDDAIKTKGYFIYPSQLFVNVAKNANINHSLNTDLAHIFAAIESSANG
EECCCCHHCCCCCCCCEECCCEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
YPSEQDIRGLFADFDTTSTRLGHTVSEKNSRLAKVLKRVAELDFGDFHNSQIDLFGDAYE
CCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHH
FLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTQVNKIYDPACGSGSLLLQAKKHFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEHHHHHHHH
EHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFNIQRGDTLTQPHFQDDKPFDAIVSN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHCC
PPYSVKWIGSDDPTLINDDRFAPAGVLAPKSKADFAFVLHALSYLSAKGRAAIVCFPGIF
CCCEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCE
YRDGAEKKIRQYLVDNNYVETVIALASNLFYGTTIAVTILVLAKNKTDTAIQFIDASGEE
ECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEECCHHH
FFKKATNTNLMTDDHIARVMEIFDRKEDVDHVAASVQYEIIVERGYNLSVSSYVEPRDTR
HHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHEEEEEEEECCCCCCHHCCCCCCCHH
EIVSIGELNAKIRTTVLRIDQLRADIDAIIAEIDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGQEQRAALQRKIWDIANDVRGSVDGWDFKQYVLGTLFYRFISENFAAYIEADDESIDY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
AALSDDVITDDIKDDAIKTKGYFIYPSQLFVNVAKNANINHSLNTDLAHIFAAIESSANG
EECCCCHHCCCCCCCCEECCCEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
YPSEQDIRGLFADFDTTSTRLGHTVSEKNSRLAKVLKRVAELDFGDFHNSQIDLFGDAYE
CCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHH
FLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTQVNKIYDPACGSGSLLLQAKKHFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEHHHHHHHH
EHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFNIQRGDTLTQPHFQDDKPFDAIVSN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHCC
PPYSVKWIGSDDPTLINDDRFAPAGVLAPKSKADFAFVLHALSYLSAKGRAAIVCFPGIF
CCCEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCE
YRDGAEKKIRQYLVDNNYVETVIALASNLFYGTTIAVTILVLAKNKTDTAIQFIDASGEE
ECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEECCHHH
FFKKATNTNLMTDDHIARVMEIFDRKEDVDHVAASVQYEIIVERGYNLSVSSYVEPRDTR
HHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHEEEEEEEECCCCCCHHCCCCCCCHH
EIVSIGELNAKIRTTVLRIDQLRADIDAIIAEIDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]