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The map label for this gene is hsdS [H]

Identifier: 86750171

GI number: 86750171

Start: 3484682

End: 3485917

Strand: Reverse

Name: hsdS [H]

Synonym: RPB_3055

Alternate gene names: 86750171

Gene position: 3485917-3484682 (Counterclockwise)

Preceding gene: 86750172

Following gene: 86750170

Centisome position: 65.38

GC content: 52.75

Gene sequence:

>1236_bases
ATGAGCGCGGCAGGGTTTCTGGAGGAGCTGCTGGATGGGGCTGATGTGGAGTGGGAACCATTGGGGGAGGTCACTCAACC
AACAGCGAACATCAAATGGTCACAAGCCGACGGCGTTTACCAATATATTGATCTCACCTCCGTCGACATCAAAACCAAAC
GCGTTACCGAGGCAAGCGAGATTACAGCCGAGACCGCGCCAAGCAGAGCGCAGAAGCTCGTTAAAGAAAATGACGTCATT
TTCGCTACGACGCGCCCCGCTCAACAACGATACTGCCTAATCGACTCCGAACTGGCCGGAAACGTCGCCAGCACGGGTTA
CTGCGTGCTCAGAGCAAAGAAGGATCAGGTACTACCTAAGTGGATTTTGCACTGGCTTGGCACAACAGAATTCAAGAATT
ACGTCGAGGAGAATCAGAGTGGGGCTGCATACCCAGCGATATCAGACGGCAAGGTGAAAGCGTTCAAAATTCCCATTCCA
TGCCCGGATGATCCGGAGAAGTCGCTGGCGATACAGGGGGAGATCGTCCGAATACTGGACACATTCACCGAGCTTACCGC
TGAGCTTACCGCGGGGCTTGCCGCCGAGCTTGCCCAGCGCAAAAAACAATACAGCCACTACCGCGACCAGCTCTTGACCT
TCAATGAAGATGAGGTGGAGTGGAAGACGCTGGGGGATATCGCGACTCTACGCCGAGGGCGAGTTATGTCGAAGGGCTAC
CTGCGAGATAACGCCGGTGTGTACCCGGTCTACAGCTCCCAAACTGCAAACAACGGCATGATTGGCCAGATCGACACGTT
TGACTTTGACGGTGAGTACGTCAGTTGGACCACAGACGGAGCAAACGCCGGAACTGTATTCTATAGAAACGAAAAATTCT
CGATTACTAACGTTTGCGGCGTAATAAAAGAAAATGGAACGTGCCCGCTGGACCTAAAATTTTTATCTTTTTGGCTTTCG
ACGGAGGCCAAGAAGCATGTTTACAGTGGAATGGGCAACCCGAAGCTGATGAGTCATCAAGTCGAGAAAATACCAATCCC
GATTCCCTTTCCAGATGACCCTAAAATATCGCTAGAAGCCCAAAAGCGCGTCGCCGCCATCCTCGACAAGTTGGATGCGC
TGACGACTTCCCTCACTGAGATCCTGCCGCGTGAAATCGAGCTGCGTGAAAAGCAGTATGCCTATTACCGCGATCAGCTG
CTGAGCTTCCCCAAGCCGGACGCGGAGGCTTTCTAA

Upstream 100 bases:

>100_bases
TTGTCAGTATTGGTGAGCTGAATGCGAAGATCAGAACCACGGTTCTGCGGATCGACCAGCTGCGCGCCGACATCGACGCC
ATCATTGCGGAGATTGACGC

Downstream 100 bases:

>100_bases
ATGGGACAGACGCTGACCGAAATTGCTAAAACGCTGCGAGATGCCGACAAGAAGGTGCAGCTGATCTATGCGTTCAATGC
CTCCGGCAAGACGCGACTGT

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoR124II; Type I restriction enzyme EcoR124II specificity protein; S protein [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSAAGFLEELLDGADVEWEPLGEVTQPTANIKWSQADGVYQYIDLTSVDIKTKRVTEASEITAETAPSRAQKLVKENDVI
FATTRPAQQRYCLIDSELAGNVASTGYCVLRAKKDQVLPKWILHWLGTTEFKNYVEENQSGAAYPAISDGKVKAFKIPIP
CPDDPEKSLAIQGEIVRILDTFTELTAELTAGLAAELAQRKKQYSHYRDQLLTFNEDEVEWKTLGDIATLRRGRVMSKGY
LRDNAGVYPVYSSQTANNGMIGQIDTFDFDGEYVSWTTDGANAGTVFYRNEKFSITNVCGVIKENGTCPLDLKFLSFWLS
TEAKKHVYSGMGNPKLMSHQVEKIPIPIPFPDDPKISLEAQKRVAAILDKLDALTTSLTEILPREIELREKQYAYYRDQL
LSFPKPDAEAF

Sequences:

>Translated_411_residues
MSAAGFLEELLDGADVEWEPLGEVTQPTANIKWSQADGVYQYIDLTSVDIKTKRVTEASEITAETAPSRAQKLVKENDVI
FATTRPAQQRYCLIDSELAGNVASTGYCVLRAKKDQVLPKWILHWLGTTEFKNYVEENQSGAAYPAISDGKVKAFKIPIP
CPDDPEKSLAIQGEIVRILDTFTELTAELTAGLAAELAQRKKQYSHYRDQLLTFNEDEVEWKTLGDIATLRRGRVMSKGY
LRDNAGVYPVYSSQTANNGMIGQIDTFDFDGEYVSWTTDGANAGTVFYRNEKFSITNVCGVIKENGTCPLDLKFLSFWLS
TEAKKHVYSGMGNPKLMSHQVEKIPIPIPFPDDPKISLEAQKRVAAILDKLDALTTSLTEILPREIELREKQYAYYRDQL
LSFPKPDAEAF
>Mature_410_residues
SAAGFLEELLDGADVEWEPLGEVTQPTANIKWSQADGVYQYIDLTSVDIKTKRVTEASEITAETAPSRAQKLVKENDVIF
ATTRPAQQRYCLIDSELAGNVASTGYCVLRAKKDQVLPKWILHWLGTTEFKNYVEENQSGAAYPAISDGKVKAFKIPIPC
PDDPEKSLAIQGEIVRILDTFTELTAELTAGLAAELAQRKKQYSHYRDQLLTFNEDEVEWKTLGDIATLRRGRVMSKGYL
RDNAGVYPVYSSQTANNGMIGQIDTFDFDGEYVSWTTDGANAGTVFYRNEKFSITNVCGVIKENGTCPLDLKFLSFWLST
EAKKHVYSGMGNPKLMSHQVEKIPIPIPFPDDPKISLEAQKRVAAILDKLDALTTSLTEILPREIELREKQYAYYRDQLL
SFPKPDAEAF

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 46008; Mature: 45877

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAGFLEELLDGADVEWEPLGEVTQPTANIKWSQADGVYQYIDLTSVDIKTKRVTEASE
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEEEEEEEHHHCCCHHH
ITAETAPSRAQKLVKENDVIFATTRPAQQRYCLIDSELAGNVASTGYCVLRAKKDQVLPK
HHHHCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHCCCCCCCCEEEEEECCCCCCHH
WILHWLGTTEFKNYVEENQSGAAYPAISDGKVKAFKIPIPCPDDPEKSLAIQGEIVRILD
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECHHHHHHHH
TFTELTAELTAGLAAELAQRKKQYSHYRDQLLTFNEDEVEWKTLGDIATLRRGRVMSKGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LRDNAGVYPVYSSQTANNGMIGQIDTFDFDGEYVSWTTDGANAGTVFYRNEKFSITNVCG
CCCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHH
VIKENGTCPLDLKFLSFWLSTEAKKHVYSGMGNPKLMSHQVEKIPIPIPFPDDPKISLEA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEHHH
QKRVAAILDKLDALTTSLTEILPREIELREKQYAYYRDQLLSFPKPDAEAF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAAGFLEELLDGADVEWEPLGEVTQPTANIKWSQADGVYQYIDLTSVDIKTKRVTEASE
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEEEEEEEHHHCCCHHH
ITAETAPSRAQKLVKENDVIFATTRPAQQRYCLIDSELAGNVASTGYCVLRAKKDQVLPK
HHHHCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHCCCCCCCCEEEEEECCCCCCHH
WILHWLGTTEFKNYVEENQSGAAYPAISDGKVKAFKIPIPCPDDPEKSLAIQGEIVRILD
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECHHHHHHHH
TFTELTAELTAGLAAELAQRKKQYSHYRDQLLTFNEDEVEWKTLGDIATLRRGRVMSKGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LRDNAGVYPVYSSQTANNGMIGQIDTFDFDGEYVSWTTDGANAGTVFYRNEKFSITNVCG
CCCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHH
VIKENGTCPLDLKFLSFWLSTEAKKHVYSGMGNPKLMSHQVEKIPIPIPFPDDPKISLEA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEHHH
QKRVAAILDKLDALTTSLTEILPREIELREKQYAYYRDQLLSFPKPDAEAF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]