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The map label for this gene is mrcA [H]

Identifier: 86750124

GI number: 86750124

Start: 3425035

End: 3427527

Strand: Reverse

Name: mrcA [H]

Synonym: RPB_3007

Alternate gene names: 86750124

Gene position: 3427527-3425035 (Counterclockwise)

Preceding gene: 86750125

Following gene: 86750123

Centisome position: 64.29

GC content: 66.31

Gene sequence:

>2493_bases
ATGCGCTTGCTGCTGCGGTTTTTCGGCTTTCTGTTCGCAGCCGGGACCATCGTGTTCCTGGTCGGCGTGGCCGCTGCGGC
CGGGATGATCTGGCATTTTTCCAAGGACTTGCCCGACTACTCTCAGCTCCAGGATTACGAACCGCCGGTGATGACGCGCG
TCCATGCGACGGACGGCTCGCTGCTCGGCGAATATTCGAAAGAGCGACGGCTGTATCTGCCGATCCAGGCCGTGCCGAAG
CTGGTGATCAACGCCTTCCTCGCCGCCGAAGACAAGAATTTCTACGAGCACGGCGGCATCGACTATCAGGGCATGGCGCG
CGCCGCGATCCTGTACGCGCAGAACATCGGGTCCAACCGGCGGCCGCAGGGTGCGTCGACGATCACCCAGCAGGTCGCCA
AGAACTTCCTGCTCACCAACGAAGTGTCGTTCACCCGTAAGATCAAGGAAGCCTTGCTGGCGATGCGGATCGAGCGGGCC
TATTCGAAGGACCGCATCCTCGAACTGTATCTCAACGAGATCTATCTGGGACTCGGCGCTTACGGCGTCGCCGCCGCCTC
ACTGGTGTATTTCGACAAATCGGTCAACGAACTGACCCTGTCGGAAGCGGCCTATCTGGCGGCGCTGCCGAAGGCCCCCG
CGGCGCTGCACCCGATCCGCAACCACGATCGCGCGGTCGAGCGCCGCAACTACGTCATCGACCGCCTGGTCGAAAACGGC
TGGACCAAGCAGGCCGACGCCGACAAGGCGCGCAAGGAGCCGCTGACCGTCACCAGCCGCGGCACCGGCGCGCACACGTT
CGCCGGCGAGTATTTCGCCGAGGAGGTCCGCCGCGATATCCTCGAGCGCTACGGCGAAAAGAAGCTCTACGAAGGCGGCC
TGTCGGTCCGCACCTCGCTCGATCCGAAGCTGCAGGTCGAGGCGCGCAAGACGATGACCGCGGGCCTCGTCAAATTCGAC
GAGGCGCAGGGCTGGCGCGGGCCGGTCTCGAAGCTGGATATCAGCGGCGACTGGGGTGTGAAGCTGGCCGACGTCAAATC
GCTGTCCGACATCTCGCCGTGGCGCATGGCTGTGGTGCTCGAGACCTCCGACCAGTCGGCGCGGATCGGCTTCCAGCCCG
GCCGCGAACTCGGCGGCGCGGTGTCGAAGAAGCGCGAGACCGGATTGATCGCTCTCGACGGCGTGAAATGGGCGAAAGCC
GCGGCGGGTCCGCAGCGCGGCCGGACGCCGGGCTCGGTGTCGCAGGTGTTGGCGGCCGGCGACGTCATCTATGCGGATTC
GCTGCTCGACAAGGAAGGCAAGCCCGTCGAGGGCCAGTACCGGCTGCGACAGATCCCCGAAGTGTCGGGCGCGATGGTGG
CGATGGATCCCTGGACCGGCCGCGTGGTGGCGATGGTCGGCGGCTTCTCGTTCGACCAGAGCCAGTTCAACCGCGCCACA
CAGGCCTATCGCCAGCCCGGCTCGTCGTTCAAGCCGCTGGTGTATTCGGCGGCGATGGACAACGGCTATACGCCGTCCAC
CGTGGTGATCGACGCGCCGATCGAAATCGATCAGGGGCAGGGCGCCGGCGTGTGGCGACCGGAGAACTATTCGACCGGCA
AATATTACGGACCGGTCACGCTGCGCAACGCGCTGCAGCGCTCGCTCAACACCGTGACGGTGCGGCTGGCGCAGGACGTC
GGCATGCCGCTGATCGGCGAATACGCCAAGCGCTTCGGCGTCTATGACGAATTGCCGAACTATCTGTCCTATGCGCTCGG
CGCCGGCGAGACCACGGTGATGCGGATGGTCACCGCCTATTCGATGTTCGCCAATGGCGGACGGCGGGTGAAGGCGACGC
TGATCGACCGCATTCAGGATCGCTACGGCCGCACCATCTTCAAGCACGACGCCCGCGAATGCCGCGGCTGCGATGCGCCG
GAGGGTTGGAAGAACCAGCCCGAGCCGCAGCTCGTCGACCGTCGCGAGCAGGTGCTCGATCCGATGACCGCCTACCAGAT
CACGTCGATGATGGAGGGCGTGGTGCAGGCCGGCACCGCGACGGTGGTGCGCGAGGTCGGCAAGCCGATCGCCGGCAAGA
CCGGCACCACCAATGACGAGAAGGATGCCTGGTTCATCGGGTTCTCGCCGGACCTCGTGGTCGGGATCTATTTCGGCTAC
GACAAGCCGCGCAATCTCGGCCGCGGCGCCACCGGCGGCCATCTCGCCGCCCCCGTCGCCAAGGATTTCTTCAAGCTCGC
GCTCGCCGACAAGCCGGCGGTGCCGTTCCGCGTGCCGGCGGGAATCAAGCTGGTCCGCGTCGACGCCAAGAGCGGGATGC
GCGCCGGCCCGGGCGACGGCGGCCGCACCATCCTCGAGGCGTTCAAGCCGGGCACCGCGCCGCCCGACAATTATTCGGTG
ATCGGCGTCGCCGATGCCGATGGCCGCCAGCAGCAGGCGCCGCCGCCGGACGATCGCGGATTTTTCCGCCCCGGCACCGG
CGGACTGTACTGA

Upstream 100 bases:

>100_bases
CGGCCCCGGCGAAGACGGGGGACTGGTTGCGGACAGTGCCAATCTCGTTCAAGGGAATTTCGCCGCGTCGCGCGGTCCTC
GGTGAAACGGAACTCAATCG

Downstream 100 bases:

>100_bases
CCGCCTTCCGGCCCAACGGGCGATTGCGCTTTGCCGCGTCGCCCGCTAAATCGCGTTTGACACATTCGCGGTCGCCTCGC
GGTGGCCGCCGCCCAGCAGA

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 830; Mature: 830

Protein sequence:

>830_residues
MRLLLRFFGFLFAAGTIVFLVGVAAAAGMIWHFSKDLPDYSQLQDYEPPVMTRVHATDGSLLGEYSKERRLYLPIQAVPK
LVINAFLAAEDKNFYEHGGIDYQGMARAAILYAQNIGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERA
YSKDRILELYLNEIYLGLGAYGVAAASLVYFDKSVNELTLSEAAYLAALPKAPAALHPIRNHDRAVERRNYVIDRLVENG
WTKQADADKARKEPLTVTSRGTGAHTFAGEYFAEEVRRDILERYGEKKLYEGGLSVRTSLDPKLQVEARKTMTAGLVKFD
EAQGWRGPVSKLDISGDWGVKLADVKSLSDISPWRMAVVLETSDQSARIGFQPGRELGGAVSKKRETGLIALDGVKWAKA
AAGPQRGRTPGSVSQVLAAGDVIYADSLLDKEGKPVEGQYRLRQIPEVSGAMVAMDPWTGRVVAMVGGFSFDQSQFNRAT
QAYRQPGSSFKPLVYSAAMDNGYTPSTVVIDAPIEIDQGQGAGVWRPENYSTGKYYGPVTLRNALQRSLNTVTVRLAQDV
GMPLIGEYAKRFGVYDELPNYLSYALGAGETTVMRMVTAYSMFANGGRRVKATLIDRIQDRYGRTIFKHDARECRGCDAP
EGWKNQPEPQLVDRREQVLDPMTAYQITSMMEGVVQAGTATVVREVGKPIAGKTGTTNDEKDAWFIGFSPDLVVGIYFGY
DKPRNLGRGATGGHLAAPVAKDFFKLALADKPAVPFRVPAGIKLVRVDAKSGMRAGPGDGGRTILEAFKPGTAPPDNYSV
IGVADADGRQQQAPPPDDRGFFRPGTGGLY

Sequences:

>Translated_830_residues
MRLLLRFFGFLFAAGTIVFLVGVAAAAGMIWHFSKDLPDYSQLQDYEPPVMTRVHATDGSLLGEYSKERRLYLPIQAVPK
LVINAFLAAEDKNFYEHGGIDYQGMARAAILYAQNIGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERA
YSKDRILELYLNEIYLGLGAYGVAAASLVYFDKSVNELTLSEAAYLAALPKAPAALHPIRNHDRAVERRNYVIDRLVENG
WTKQADADKARKEPLTVTSRGTGAHTFAGEYFAEEVRRDILERYGEKKLYEGGLSVRTSLDPKLQVEARKTMTAGLVKFD
EAQGWRGPVSKLDISGDWGVKLADVKSLSDISPWRMAVVLETSDQSARIGFQPGRELGGAVSKKRETGLIALDGVKWAKA
AAGPQRGRTPGSVSQVLAAGDVIYADSLLDKEGKPVEGQYRLRQIPEVSGAMVAMDPWTGRVVAMVGGFSFDQSQFNRAT
QAYRQPGSSFKPLVYSAAMDNGYTPSTVVIDAPIEIDQGQGAGVWRPENYSTGKYYGPVTLRNALQRSLNTVTVRLAQDV
GMPLIGEYAKRFGVYDELPNYLSYALGAGETTVMRMVTAYSMFANGGRRVKATLIDRIQDRYGRTIFKHDARECRGCDAP
EGWKNQPEPQLVDRREQVLDPMTAYQITSMMEGVVQAGTATVVREVGKPIAGKTGTTNDEKDAWFIGFSPDLVVGIYFGY
DKPRNLGRGATGGHLAAPVAKDFFKLALADKPAVPFRVPAGIKLVRVDAKSGMRAGPGDGGRTILEAFKPGTAPPDNYSV
IGVADADGRQQQAPPPDDRGFFRPGTGGLY
>Mature_830_residues
MRLLLRFFGFLFAAGTIVFLVGVAAAAGMIWHFSKDLPDYSQLQDYEPPVMTRVHATDGSLLGEYSKERRLYLPIQAVPK
LVINAFLAAEDKNFYEHGGIDYQGMARAAILYAQNIGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERA
YSKDRILELYLNEIYLGLGAYGVAAASLVYFDKSVNELTLSEAAYLAALPKAPAALHPIRNHDRAVERRNYVIDRLVENG
WTKQADADKARKEPLTVTSRGTGAHTFAGEYFAEEVRRDILERYGEKKLYEGGLSVRTSLDPKLQVEARKTMTAGLVKFD
EAQGWRGPVSKLDISGDWGVKLADVKSLSDISPWRMAVVLETSDQSARIGFQPGRELGGAVSKKRETGLIALDGVKWAKA
AAGPQRGRTPGSVSQVLAAGDVIYADSLLDKEGKPVEGQYRLRQIPEVSGAMVAMDPWTGRVVAMVGGFSFDQSQFNRAT
QAYRQPGSSFKPLVYSAAMDNGYTPSTVVIDAPIEIDQGQGAGVWRPENYSTGKYYGPVTLRNALQRSLNTVTVRLAQDV
GMPLIGEYAKRFGVYDELPNYLSYALGAGETTVMRMVTAYSMFANGGRRVKATLIDRIQDRYGRTIFKHDARECRGCDAP
EGWKNQPEPQLVDRREQVLDPMTAYQITSMMEGVVQAGTATVVREVGKPIAGKTGTTNDEKDAWFIGFSPDLVVGIYFGY
DKPRNLGRGATGGHLAAPVAKDFFKLALADKPAVPFRVPAGIKLVRVDAKSGMRAGPGDGGRTILEAFKPGTAPPDNYSV
IGVADADGRQQQAPPPDDRGFFRPGTGGLY

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=859, Percent_Identity=34.1094295692666, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1786343, Length=274, Percent_Identity=35.7664233576642, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1788867, Length=210, Percent_Identity=35.2380952380952, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789601, Length=129, Percent_Identity=46.5116279069767, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 90826; Mature: 90826

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLLRFFGFLFAAGTIVFLVGVAAAAGMIWHFSKDLPDYSQLQDYEPPVMTRVHATDGS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCH
LLGEYSKERRLYLPIQAVPKLVINAFLAAEDKNFYEHGGIDYQGMARAAILYAQNIGSNR
HHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCC
RPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERAYSKDRILELYLNEIYLGLGA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCH
YGVAAASLVYFDKSVNELTLSEAAYLAALPKAPAALHPIRNHDRAVERRNYVIDRLVENG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCC
WTKQADADKARKEPLTVTSRGTGAHTFAGEYFAEEVRRDILERYGEKKLYEGGLSVRTSL
CCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECC
DPKLQVEARKTMTAGLVKFDEAQGWRGPVSKLDISGDWGVKLADVKSLSDISPWRMAVVL
CCCEEEHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCCEEEECHHCCCCCCCEEEEEEE
ETSDQSARIGFQPGRELGGAVSKKRETGLIALDGVKWAKAAAGPQRGRTPGSVSQVLAAG
ECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHC
DVIYADSLLDKEGKPVEGQYRLRQIPEVSGAMVAMDPWTGRVVAMVGGFSFDQSQFNRAT
CEEEEHHHHCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHH
QAYRQPGSSFKPLVYSAAMDNGYTPSTVVIDAPIEIDQGQGAGVWRPENYSTGKYYGPVT
HHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCEECCHH
LRNALQRSLNTVTVRLAQDVGMPLIGEYAKRFGVYDELPNYLSYALGAGETTVMRMVTAY
HHHHHHHHHHHEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SMFANGGRRVKATLIDRIQDRYGRTIFKHDARECRGCDAPEGWKNQPEPQLVDRREQVLD
HHHHCCCCEEHHHHHHHHHHHHCCHHHHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
PMTAYQITSMMEGVVQAGTATVVREVGKPIAGKTGTTNDEKDAWFIGFSPDLVVGIYFGY
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECC
DKPRNLGRGATGGHLAAPVAKDFFKLALADKPAVPFRVPAGIKLVRVDAKSGMRAGPGDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEECCCCCCCCCCCC
GRTILEAFKPGTAPPDNYSVIGVADADGRQQQAPPPDDRGFFRPGTGGLY
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLLLRFFGFLFAAGTIVFLVGVAAAAGMIWHFSKDLPDYSQLQDYEPPVMTRVHATDGS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCH
LLGEYSKERRLYLPIQAVPKLVINAFLAAEDKNFYEHGGIDYQGMARAAILYAQNIGSNR
HHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCC
RPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRIERAYSKDRILELYLNEIYLGLGA
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCH
YGVAAASLVYFDKSVNELTLSEAAYLAALPKAPAALHPIRNHDRAVERRNYVIDRLVENG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCC
WTKQADADKARKEPLTVTSRGTGAHTFAGEYFAEEVRRDILERYGEKKLYEGGLSVRTSL
CCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECC
DPKLQVEARKTMTAGLVKFDEAQGWRGPVSKLDISGDWGVKLADVKSLSDISPWRMAVVL
CCCEEEHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCCEEEECHHCCCCCCCEEEEEEE
ETSDQSARIGFQPGRELGGAVSKKRETGLIALDGVKWAKAAAGPQRGRTPGSVSQVLAAG
ECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHC
DVIYADSLLDKEGKPVEGQYRLRQIPEVSGAMVAMDPWTGRVVAMVGGFSFDQSQFNRAT
CEEEEHHHHCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHH
QAYRQPGSSFKPLVYSAAMDNGYTPSTVVIDAPIEIDQGQGAGVWRPENYSTGKYYGPVT
HHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCEECCHH
LRNALQRSLNTVTVRLAQDVGMPLIGEYAKRFGVYDELPNYLSYALGAGETTVMRMVTAY
HHHHHHHHHHHEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SMFANGGRRVKATLIDRIQDRYGRTIFKHDARECRGCDAPEGWKNQPEPQLVDRREQVLD
HHHHCCCCEEHHHHHHHHHHHHCCHHHHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
PMTAYQITSMMEGVVQAGTATVVREVGKPIAGKTGTTNDEKDAWFIGFSPDLVVGIYFGY
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECC
DKPRNLGRGATGGHLAAPVAKDFFKLALADKPAVPFRVPAGIKLVRVDAKSGMRAGPGDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEECCCCCCCCCCCC
GRTILEAFKPGTAPPDNYSVIGVADADGRQQQAPPPDDRGFFRPGTGGLY
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA