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The map label for this gene is amiC [H]

Identifier: 86750125

GI number: 86750125

Start: 3427721

End: 3429031

Strand: Reverse

Name: amiC [H]

Synonym: RPB_3008

Alternate gene names: 86750125

Gene position: 3429031-3427721 (Counterclockwise)

Preceding gene: 86750127

Following gene: 86750124

Centisome position: 64.31

GC content: 65.29

Gene sequence:

>1311_bases
TTGCAGGGGCGCGCAAATCATTTTGTTTTGTTCAGCGCCGTCTTCGCGTGCGCTGCAGCATGGATGTGCGCGTCGCCGAC
CCAGGGGTGGGCCGTGGAAGCCCTGCCTGCCCCCTCGACCCCAACCCAAAGCACTGCAGCGCCGGTCACAAACGGCTTTC
CGATCGCGTCCGATGCCCGTCTGGCGGGTGACGAGAAGCAGACCCGGTTCATCGTCGATTTCGACACCAAGGTTCCGATC
CGCGCGTTCGCGCTGGCGGATCCGTATCGCATCGTCATCGATCTGCCGCAGATCAATTTCCGCCTGCCGTCGGCCGCCAA
TGGTGCAAGTCGTGGCCTGATCAAGGCTTTTCGGTACGGCCTGGTGATGCCCGGCGGCTCGCGGATCGTGCTCGAACTGG
CGGGGCCGGCGAAGATCGCCAAGGCCGATATGCTCGACGCAGCCAATGGGCAGCCGGCGCGGCTGGTGATCGAACTCGAT
TCGGTTGACCGCACGGCCTTCGTCGCGGCGCTTAGTGCCGAGAAGGCGCCCGAACTGCGACCTTCGGTCAGTATGGCGGA
TGCGACCTCGTCAGTGCCAGCTGCCGATGCGGCGAAGGACGATCCGCGGCCGGTCGTCGTGCTCGATCCGGGACATGGCG
GGATCGACAACGGCACCCAATCCGCCAGTGGCATCGCCGAGAAGACGCTGGTGCTGGATTTCGCGCTGGCGTTGCGCGAC
CAGATGGAAAAGGGCGGCAAGTACCGCGTGGTGCTGACCCGCGCCGACGACACCTTCATTCCGCTCAACGACCGGGTGAA
GATCGCGCGCGCGCAGTCCGCCGCGCTGTTCGTGTCGATTCATGCCGACGCGCTGCCGCGCGGCGAGGGCGATGCCCAGG
GCGCCACCATCTACACGCTGTCCGACAGGGCCTCCGATGCCGAGGCGCAGCGGCTGGCGGATGCCGAAAACAGGGCCGAC
GCGATCGGCGGGGTCGATCTGACCGAAGAGCCGACCGAGGTCGCCGATATCCTGATCGACCTCGCGCAGCGCGAGACCAA
GACGTTCTCGAACAGCTTCGCCCGGACCTTGATGCGGGAAATGAAGGGCGCGACCCGGCTGCACAAGAATCCTCTCAAAT
CCGCCGGCTTCCGGGTCCTGAAGGCGCCCGACGTGCCGTCGGTGCTGATCGAACTCGGCTATGTCTCCAACAAAGGCGAC
CTCAAGCAGCTGATTTCCGAACAGTGGCGCACCAAGACCGTCGGCGCGGTCTCCCAGGCGATCGATTCGTTTTTCGCCAG
GCGGTTGGTCTCGGCCGGAAAGCCGAACTGA

Upstream 100 bases:

>100_bases
CCGGCAACACTGCCTATTATTCGAATCGCGCGCCTTAAGCCGCGATTAACCACGCACTTCTAATGCAGTAAAAGGACCAG
CGGAGAGGGGTCGGGATTCG

Downstream 100 bases:

>100_bases
CGCGGCATTGCGCGTGGCCGGGCCCTAGTTTGGCCACAGCGGAAGTTCTATAAGACCACGTTGCAGTTCGCGATTTCGGT
GACGAAACGAGGCCGGCCCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQGRANHFVLFSAVFACAAAWMCASPTQGWAVEALPAPSTPTQSTAAPVTNGFPIASDARLAGDEKQTRFIVDFDTKVPI
RAFALADPYRIVIDLPQINFRLPSAANGASRGLIKAFRYGLVMPGGSRIVLELAGPAKIAKADMLDAANGQPARLVIELD
SVDRTAFVAALSAEKAPELRPSVSMADATSSVPAADAAKDDPRPVVVLDPGHGGIDNGTQSASGIAEKTLVLDFALALRD
QMEKGGKYRVVLTRADDTFIPLNDRVKIARAQSAALFVSIHADALPRGEGDAQGATIYTLSDRASDAEAQRLADAENRAD
AIGGVDLTEEPTEVADILIDLAQRETKTFSNSFARTLMREMKGATRLHKNPLKSAGFRVLKAPDVPSVLIELGYVSNKGD
LKQLISEQWRTKTVGAVSQAIDSFFARRLVSAGKPN

Sequences:

>Translated_436_residues
MQGRANHFVLFSAVFACAAAWMCASPTQGWAVEALPAPSTPTQSTAAPVTNGFPIASDARLAGDEKQTRFIVDFDTKVPI
RAFALADPYRIVIDLPQINFRLPSAANGASRGLIKAFRYGLVMPGGSRIVLELAGPAKIAKADMLDAANGQPARLVIELD
SVDRTAFVAALSAEKAPELRPSVSMADATSSVPAADAAKDDPRPVVVLDPGHGGIDNGTQSASGIAEKTLVLDFALALRD
QMEKGGKYRVVLTRADDTFIPLNDRVKIARAQSAALFVSIHADALPRGEGDAQGATIYTLSDRASDAEAQRLADAENRAD
AIGGVDLTEEPTEVADILIDLAQRETKTFSNSFARTLMREMKGATRLHKNPLKSAGFRVLKAPDVPSVLIELGYVSNKGD
LKQLISEQWRTKTVGAVSQAIDSFFARRLVSAGKPN
>Mature_436_residues
MQGRANHFVLFSAVFACAAAWMCASPTQGWAVEALPAPSTPTQSTAAPVTNGFPIASDARLAGDEKQTRFIVDFDTKVPI
RAFALADPYRIVIDLPQINFRLPSAANGASRGLIKAFRYGLVMPGGSRIVLELAGPAKIAKADMLDAANGQPARLVIELD
SVDRTAFVAALSAEKAPELRPSVSMADATSSVPAADAAKDDPRPVVVLDPGHGGIDNGTQSASGIAEKTLVLDFALALRD
QMEKGGKYRVVLTRADDTFIPLNDRVKIARAQSAALFVSIHADALPRGEGDAQGATIYTLSDRASDAEAQRLADAENRAD
AIGGVDLTEEPTEVADILIDLAQRETKTFSNSFARTLMREMKGATRLHKNPLKSAGFRVLKAPDVPSVLIELGYVSNKGD
LKQLISEQWRTKTVGAVSQAIDSFFARRLVSAGKPN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=373, Percent_Identity=33.5120643431635, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788776, Length=230, Percent_Identity=39.5652173913043, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790611, Length=428, Percent_Identity=25.9345794392523, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46377; Mature: 46377

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGRANHFVLFSAVFACAAAWMCASPTQGWAVEALPAPSTPTQSTAAPVTNGFPIASDAR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LAGDEKQTRFIVDFDTKVPIRAFALADPYRIVIDLPQINFRLPSAANGASRGLIKAFRYG
CCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHC
LVMPGGSRIVLELAGPAKIAKADMLDAANGQPARLVIELDSVDRTAFVAALSAEKAPELR
EECCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCC
PSVSMADATSSVPAADAAKDDPRPVVVLDPGHGGIDNGTQSASGIAEKTLVLDFALALRD
CCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QMEKGGKYRVVLTRADDTFIPLNDRVKIARAQSAALFVSIHADALPRGEGDAQGATIYTL
HHHCCCCEEEEEEECCCCEECCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEE
SDRASDAEAQRLADAENRADAIGGVDLTEEPTEVADILIDLAQRETKTFSNSFARTLMRE
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGATRLHKNPLKSAGFRVLKAPDVPSVLIELGYVSNKGDLKQLISEQWRTKTVGAVSQA
HHHHHHHHCCCHHHHCEEEEECCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
IDSFFARRLVSAGKPN
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQGRANHFVLFSAVFACAAAWMCASPTQGWAVEALPAPSTPTQSTAAPVTNGFPIASDAR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LAGDEKQTRFIVDFDTKVPIRAFALADPYRIVIDLPQINFRLPSAANGASRGLIKAFRYG
CCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHC
LVMPGGSRIVLELAGPAKIAKADMLDAANGQPARLVIELDSVDRTAFVAALSAEKAPELR
EECCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCC
PSVSMADATSSVPAADAAKDDPRPVVVLDPGHGGIDNGTQSASGIAEKTLVLDFALALRD
CCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QMEKGGKYRVVLTRADDTFIPLNDRVKIARAQSAALFVSIHADALPRGEGDAQGATIYTL
HHHCCCCEEEEEEECCCCEECCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEE
SDRASDAEAQRLADAENRADAIGGVDLTEEPTEVADILIDLAQRETKTFSNSFARTLMRE
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGATRLHKNPLKSAGFRVLKAPDVPSVLIELGYVSNKGDLKQLISEQWRTKTVGAVSQA
HHHHHHHHCCCHHHHCEEEEECCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
IDSFFARRLVSAGKPN
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]