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The map label for this gene is prfB [H]

Identifier: 86750123

GI number: 86750123

Start: 3423797

End: 3424765

Strand: Reverse

Name: prfB [H]

Synonym: RPB_3006

Alternate gene names: 86750123

Gene position: 3424765-3423797 (Counterclockwise)

Preceding gene: 86750124

Following gene: 86750122

Centisome position: 64.23

GC content: 66.56

Gene sequence:

>969_bases
ATGCAGGAGCGCACCTCGCTGGAGGATTCGCTCACCGGCATCGGCAAGGTCGAGCGTGAGCTCAGCGACAATATCGAGAT
GATCGAACTCGGCGAGGCCGAGGGCGACGAGGGCGTCGTCCGCGAGGCCGAGGCTGCGCTCAAGGCGCTGAAGAAGGAAG
TCGCGCGGCGCGAACTCGAAGCGCTGCTGTCCGGCGAGGCCGATCGCTTCGACACTTATCTCGAAGTCCACGCCGGCGCC
GGCGGCACCGAGAGCCAGGACTGGGCCTCGATGCTGCTGCGGATGTACACGCGCTGGGCCGAGAAGCACGGCTTCAAGGT
CGAGTATCTCGAAGAATCCGAGGGCGAAGAGGCGGGGATCAAATCGGCGACGATCCAGATCTCGGGTCACAACGCCTATG
GCTGGCTCAAGACCGAGGCCGGCGTGCACCGGCTGGTGCGCATCTCGCCGTTCGATTCCAACGCGCGGCGGCACACCTCG
TTCTCGTCGGTGCAGATTTTCCCGGTGATCGACAATTCGATCAAGATCGACATCGCCGAGTCCGACGTCCGGGTCGACAC
GATGCGCTCCGGCGGCGCCGGCGGCCAGCACGTCAACAAGACCGAATCCGCGGTGCGCCTGACGCACGTCCCGACCGGCG
TCGCGGTGGTCTGTCAGGCCGGCCGCTCGCAGCACAAGAACCGCGCCCAGGCGTGGGACATGCTGCGCGCGCGTCTTTAC
GAAATCGAGCTGAAGAAGCGCGAAGAGCAGGCTGCCGCCGATCAGGCCGCCAAGACCGAAATCGGCTGGGGCCACCAGAT
CCGCTCCTACGTGCTGCAGCCCTACCAGATGGTCAAGGACCTGCGCACCGGCGTGCAGACCTCCGACACCGCCGGCGTGC
TCGACGGCGACCTCGACGAATTCATGGCCGCGACCCTGGCGCAGCGCGCGTTCGGCACGGGGCCGGGCAGTGTCGAGGAC
GTGGACTAA

Upstream 100 bases:

>100_bases
CATCTTTGACGTCGACCAGTCGACCGTCCGCCTCGCGGAGCTGAACAAGCTCGCCGAGGATCCAGACCTCTGGAACGATC
CGCAGAAGGCCCAGAAGCTG

Downstream 100 bases:

>100_bases
GCGTGGGCGCTCCCACAAAGGTCGGCTTCATCGGTCTCGGCAAGATGGGCCATGGCATGGCCGGCCGGCTGCTCGACGCC
GGCTTCGACGTCGCGGTATG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAEGDEGVVREAEAALKALKKEVARRELEALLSGEADRFDTYLEVHAGA
GGTESQDWASMLLRMYTRWAEKHGFKVEYLEESEGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTS
FSSVQIFPVIDNSIKIDIAESDVRVDTMRSGGAGGQHVNKTESAVRLTHVPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
EIELKKREEQAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTAGVLDGDLDEFMAATLAQRAFGTGPGSVED
VD

Sequences:

>Translated_322_residues
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAEGDEGVVREAEAALKALKKEVARRELEALLSGEADRFDTYLEVHAGA
GGTESQDWASMLLRMYTRWAEKHGFKVEYLEESEGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTS
FSSVQIFPVIDNSIKIDIAESDVRVDTMRSGGAGGQHVNKTESAVRLTHVPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
EIELKKREEQAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTAGVLDGDLDEFMAATLAQRAFGTGPGSVED
VD
>Mature_322_residues
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAEGDEGVVREAEAALKALKKEVARRELEALLSGEADRFDTYLEVHAGA
GGTESQDWASMLLRMYTRWAEKHGFKVEYLEESEGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTS
FSSVQIFPVIDNSIKIDIAESDVRVDTMRSGGAGGQHVNKTESAVRLTHVPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
EIELKKREEQAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTAGVLDGDLDEFMAATLAQRAFGTGPGSVED
VD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=300, Percent_Identity=36, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI34577120, Length=261, Percent_Identity=37.1647509578544, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI166795305, Length=213, Percent_Identity=35.2112676056338, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=55.0819672131148, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1787462, Length=238, Percent_Identity=41.5966386554622, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=226, Percent_Identity=33.1858407079646, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=243, Percent_Identity=40.3292181069959, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=40.3061224489796, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35478; Mature: 35478

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01181 RIBOSOMAL_S21 ; PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAEGDEGVVREAEAALKALKKEVARRELE
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALLSGEADRFDTYLEVHAGAGGTESQDWASMLLRMYTRWAEKHGFKVEYLEESEGEEAGI
HHHCCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
KSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSVQIFPVIDNSIKIDIAE
CEEEEEEECCCCCEEEECHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEC
SDVRVDTMRSGGAGGQHVNKTESAVRLTHVPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
CCCEEEECCCCCCCCCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIELKKREEQAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTAGVLDGDLDE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
FMAATLAQRAFGTGPGSVEDVD
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAEGDEGVVREAEAALKALKKEVARRELE
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALLSGEADRFDTYLEVHAGAGGTESQDWASMLLRMYTRWAEKHGFKVEYLEESEGEEAGI
HHHCCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
KSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSVQIFPVIDNSIKIDIAE
CEEEEEEECCCCCEEEECHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEC
SDVRVDTMRSGGAGGQHVNKTESAVRLTHVPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
CCCEEEECCCCCCCCCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIELKKREEQAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTAGVLDGDLDE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
FMAATLAQRAFGTGPGSVEDVD
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA