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The map label for this gene is garR [H]

Identifier: 86750122

GI number: 86750122

Start: 3422907

End: 3423794

Strand: Reverse

Name: garR [H]

Synonym: RPB_3005

Alternate gene names: 86750122

Gene position: 3423794-3422907 (Counterclockwise)

Preceding gene: 86750123

Following gene: 86750121

Centisome position: 64.22

GC content: 67.34

Gene sequence:

>888_bases
GTGGGCGCTCCCACAAAGGTCGGCTTCATCGGTCTCGGCAAGATGGGCCATGGCATGGCCGGCCGGCTGCTCGACGCCGG
CTTCGACGTCGCGGTATGGAATCGCAATCCGGCAAAGGCCGACGCTCTGATCAGCCGCGGCGCGCGCCGCGCTGCGACGC
CGGCCGAGGCCGCACACGCCGCCGATGCGATCGTGACCATGGTCGCCGATGACGAAGCGTCGCAAGCCGTCTGGCTCGGC
GCCGATGGTGTCGCGACGACGGCCAAGGTTGGTGCTGTCGCGATCGAATGTTCGACCGTCTCGCATCAACATGCGCTCGA
ACTCGCCGCGGCGCTGCGCGCGCGTGGGTTGATCTACATCGATTGTCCCGTCACGGGCCTGCCCGACGCTGCCGCACGCG
GCGAACTCACCTTGCTGATTGGCGCCGAGCCGAACGATCTCGACGCGGCGCGCCCCTATCTTGCGCCGCTTGGCCGGACG
ATCCGGCATTTCGGTCCGGTCGGGTCCGGCACTGTCTACAAGCTGATCAACAATCTGATGGGCGCGATCCAGATCGCCGG
CCTTGCCGAAGGCATCGCGATCGCGGAGCGCGCCGGACTCGACATGGCACTGGTGCGTGAAGCGATCGCGGGCAGTGTCG
TTGCCAGTCCGCAGGTGCTGCGGCAGGCACCTCGGATGATCGACCGCGACTTCACCAACGCCACCTTTACCGCGGGGCTC
CGGCACAAAGACGCGCGCTACGCGATCGAACTGGCGAAAGCGCTGCGATTGGAGCTGCCGCTGTCGCTCGCAGCGGTCGC
GAGTTATGCGCACGCGACCGATGCCGCGCCCGACGACGACGAGGCAAAGATCGTCGAGATCGTCTCGAAGCCACGCCCGC
GCGAGTAA

Upstream 100 bases:

>100_bases
CCGGCGTGCTCGACGGCGACCTCGACGAATTCATGGCCGCGACCCTGGCGCAGCGCGCGTTCGGCACGGGGCCGGGCAGT
GTCGAGGACGTGGACTAAGC

Downstream 100 bases:

>100_bases
CTCCCGGCACCGCGCCGTTGAACGGCCGTGCGCAACCGGCTTTCGGAATTCGGGTGGCGGCGTGACACTCTCTGCAGGAT
GACTGGCGTCGCGAGGTGCG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MGAPTKVGFIGLGKMGHGMAGRLLDAGFDVAVWNRNPAKADALISRGARRAATPAEAAHAADAIVTMVADDEASQAVWLG
ADGVATTAKVGAVAIECSTVSHQHALELAAALRARGLIYIDCPVTGLPDAAARGELTLLIGAEPNDLDAARPYLAPLGRT
IRHFGPVGSGTVYKLINNLMGAIQIAGLAEGIAIAERAGLDMALVREAIAGSVVASPQVLRQAPRMIDRDFTNATFTAGL
RHKDARYAIELAKALRLELPLSLAAVASYAHATDAAPDDDEAKIVEIVSKPRPRE

Sequences:

>Translated_295_residues
MGAPTKVGFIGLGKMGHGMAGRLLDAGFDVAVWNRNPAKADALISRGARRAATPAEAAHAADAIVTMVADDEASQAVWLG
ADGVATTAKVGAVAIECSTVSHQHALELAAALRARGLIYIDCPVTGLPDAAARGELTLLIGAEPNDLDAARPYLAPLGRT
IRHFGPVGSGTVYKLINNLMGAIQIAGLAEGIAIAERAGLDMALVREAIAGSVVASPQVLRQAPRMIDRDFTNATFTAGL
RHKDARYAIELAKALRLELPLSLAAVASYAHATDAAPDDDEAKIVEIVSKPRPRE
>Mature_294_residues
GAPTKVGFIGLGKMGHGMAGRLLDAGFDVAVWNRNPAKADALISRGARRAATPAEAAHAADAIVTMVADDEASQAVWLGA
DGVATTAKVGAVAIECSTVSHQHALELAAALRARGLIYIDCPVTGLPDAAARGELTLLIGAEPNDLDAARPYLAPLGRTI
RHFGPVGSGTVYKLINNLMGAIQIAGLAEGIAIAERAGLDMALVREAIAGSVVASPQVLRQAPRMIDRDFTNATFTAGLR
HKDARYAIELAKALRLELPLSLAAVASYAHATDAAPDDDEAKIVEIVSKPRPRE

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=276, Percent_Identity=30.0724637681159, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI23308751, Length=203, Percent_Identity=33.0049261083744, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI145693186, Length=261, Percent_Identity=33.7164750957854, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786719, Length=255, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790315, Length=266, Percent_Identity=30.4511278195489, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789092, Length=298, Percent_Identity=29.5302013422819, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557316, Length=211, Percent_Identity=34.1232227488152, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=30.8474576271186, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=30.8474576271186, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574115, Length=266, Percent_Identity=24.4360902255639, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 30643; Mature: 30512

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAPTKVGFIGLGKMGHGMAGRLLDAGFDVAVWNRNPAKADALISRGARRAATPAEAAHA
CCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHH
ADAIVTMVADDEASQAVWLGADGVATTAKVGAVAIECSTVSHQHALELAAALRARGLIYI
HHHEEEEEECCCCCCEEEECCCCCCHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCEEEE
DCPVTGLPDAAARGELTLLIGAEPNDLDAARPYLAPLGRTIRHFGPVGSGTVYKLINNLM
ECCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GAIQIAGLAEGIAIAERAGLDMALVREAIAGSVVASPQVLRQAPRMIDRDFTNATFTAGL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCCEEECCC
RHKDARYAIELAKALRLELPLSLAAVASYAHATDAAPDDDEAKIVEIVSKPRPRE
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GAPTKVGFIGLGKMGHGMAGRLLDAGFDVAVWNRNPAKADALISRGARRAATPAEAAHA
CCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHH
ADAIVTMVADDEASQAVWLGADGVATTAKVGAVAIECSTVSHQHALELAAALRARGLIYI
HHHEEEEEECCCCCCEEEECCCCCCHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCEEEE
DCPVTGLPDAAARGELTLLIGAEPNDLDAARPYLAPLGRTIRHFGPVGSGTVYKLINNLM
ECCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GAIQIAGLAEGIAIAERAGLDMALVREAIAGSVVASPQVLRQAPRMIDRDFTNATFTAGL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCCEEECCC
RHKDARYAIELAKALRLELPLSLAAVASYAHATDAAPDDDEAKIVEIVSKPRPRE
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]