Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 86750056

GI number: 86750056

Start: 3351558

End: 3352319

Strand: Reverse

Name: cobB [H]

Synonym: RPB_2939

Alternate gene names: 86750056

Gene position: 3352319-3351558 (Counterclockwise)

Preceding gene: 86750058

Following gene: 86750055

Centisome position: 62.88

GC content: 67.19

Gene sequence:

>762_bases
ATGATCGCACCCGACCTGCGTAGCGGCGTCGAGCGGCTCGGCGACATGATCGCCGGCGCCTCGGTGATCGTGCCGTTCAC
CGGCGCCGGGATTTCCACCGAATCCGGCATTCCGGATTTTCGCTCGCCGGGCGGGCTGTGGACCCGCAACCGGCCGATCC
CGTTCGAGGAATTCGTCGCGCGGCAGGATGCGCGCGACGAGGCCTGGCGGCGGCGCTTCGCGATGGACGACACCTTCGCG
GCGGCGCAGCCGGGACGCGGCCATCGCGCGCTCGCGGCGCTGTACAAGGCCGGCAAGGTGCCGGCGATCATCACCCAGAA
CATCGACAATCTGCATCAGGCTTCCGGCTTCGCCGCTGGCGATGTCGTGGAGCTGCACGGTAACACCACCTATGCGCGCT
GCATCGGCTGCGGCAAACGCCACGAGCTCGATTGGGTGCGGCAGTGGTTTCGACGCGCCGGCCATGCGCCGCATTGCACC
GCCTGCGACGAGCCGGTGAAGACCGCGACGATCTCGTTCGGCCAGGCGATGCCCGTCGACGAGATGCGCTACGCGAGCCA
GCTCGCGCAGAATTGCGATTTGTTTCTGGTGATCGGATCGTCGCTGGTGGTGTGGCCGGCCGCGGGGATTCCGATGCTCG
CGAAAGAGTCCGGCGCCAAGCTCGTGATCATCAACAACGAGCCGACCGAGCAGGACGAGATCGCCGATCTGGTGATCCGG
CACGACATCGGCGAGACGCTCGGACCGTTCGTCGGCAACTGA

Upstream 100 bases:

>100_bases
AGGCGCGTGGTCGAGCCGGTCGCATATCCGGGCCGCGTCCGATCGGCTTGCGGAAGGCGCGGCAAATTGCGAGATGTCGA
ATGTCACGGGACTGGAAGCC

Downstream 100 bases:

>100_bases
ATCCCCGTATGATTCGCAGGTATTCAAGCCTGTTCATAGCTGTCGCATCACGCGTTTTTTGTCTTTGCGGCGGGCATCGC
GGTGTTATCTTTAGATTAAT

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MIAPDLRSGVERLGDMIAGASVIVPFTGAGISTESGIPDFRSPGGLWTRNRPIPFEEFVARQDARDEAWRRRFAMDDTFA
AAQPGRGHRALAALYKAGKVPAIITQNIDNLHQASGFAAGDVVELHGNTTYARCIGCGKRHELDWVRQWFRRAGHAPHCT
ACDEPVKTATISFGQAMPVDEMRYASQLAQNCDLFLVIGSSLVVWPAAGIPMLAKESGAKLVIINNEPTEQDEIADLVIR
HDIGETLGPFVGN

Sequences:

>Translated_253_residues
MIAPDLRSGVERLGDMIAGASVIVPFTGAGISTESGIPDFRSPGGLWTRNRPIPFEEFVARQDARDEAWRRRFAMDDTFA
AAQPGRGHRALAALYKAGKVPAIITQNIDNLHQASGFAAGDVVELHGNTTYARCIGCGKRHELDWVRQWFRRAGHAPHCT
ACDEPVKTATISFGQAMPVDEMRYASQLAQNCDLFLVIGSSLVVWPAAGIPMLAKESGAKLVIINNEPTEQDEIADLVIR
HDIGETLGPFVGN
>Mature_253_residues
MIAPDLRSGVERLGDMIAGASVIVPFTGAGISTESGIPDFRSPGGLWTRNRPIPFEEFVARQDARDEAWRRRFAMDDTFA
AAQPGRGHRALAALYKAGKVPAIITQNIDNLHQASGFAAGDVVELHGNTTYARCIGCGKRHELDWVRQWFRRAGHAPHCT
ACDEPVKTATISFGQAMPVDEMRYASQLAQNCDLFLVIGSSLVVWPAAGIPMLAKESGAKLVIINNEPTEQDEIADLVIR
HDIGETLGPFVGN

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300797577, Length=251, Percent_Identity=31.8725099601594, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI6912662, Length=267, Percent_Identity=30.7116104868914, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI300797597, Length=244, Percent_Identity=32.3770491803279, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI7657575, Length=231, Percent_Identity=31.6017316017316, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI300795542, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI13775602, Length=218, Percent_Identity=31.1926605504587, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI13775600, Length=217, Percent_Identity=30.4147465437788, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI63054862, Length=202, Percent_Identity=31.6831683168317, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI6912660, Length=206, Percent_Identity=31.5533980582524, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI7706712, Length=247, Percent_Identity=28.7449392712551, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI6912664, Length=255, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI13787215, Length=237, Percent_Identity=28.2700421940928, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI300797705, Length=187, Percent_Identity=31.0160427807487, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI215982798, Length=163, Percent_Identity=32.5153374233129, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI308199517, Length=217, Percent_Identity=35.9447004608295, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567771, Length=272, Percent_Identity=27.5735294117647, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71990482, Length=272, Percent_Identity=26.8382352941176, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71990487, Length=274, Percent_Identity=26.6423357664234, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17541892, Length=207, Percent_Identity=31.4009661835749, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17505510, Length=232, Percent_Identity=28.448275862069, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325242, Length=213, Percent_Identity=30.5164319248826, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28571445, Length=277, Percent_Identity=31.7689530685921, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645650, Length=242, Percent_Identity=28.9256198347107, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648389, Length=200, Percent_Identity=32, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137536, Length=225, Percent_Identity=29.7777777777778, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650933, Length=255, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27477; Mature: 27477

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPDLRSGVERLGDMIAGASVIVPFTGAGISTESGIPDFRSPGGLWTRNRPIPFEEFVA
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RQDARDEAWRRRFAMDDTFAAAQPGRGHRALAALYKAGKVPAIITQNIDNLHQASGFAAG
HCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCC
DVVELHGNTTYARCIGCGKRHELDWVRQWFRRAGHAPHCTACDEPVKTATISFGQAMPVD
CEEEECCCCEEHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHH
EMRYASQLAQNCDLFLVIGSSLVVWPAAGIPMLAKESGAKLVIINNEPTEQDEIADLVIR
HHHHHHHHHHCCCEEEEECCCEEEECCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHH
HDIGETLGPFVGN
HHHHHHHHHCCCC
>Mature Secondary Structure
MIAPDLRSGVERLGDMIAGASVIVPFTGAGISTESGIPDFRSPGGLWTRNRPIPFEEFVA
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RQDARDEAWRRRFAMDDTFAAAQPGRGHRALAALYKAGKVPAIITQNIDNLHQASGFAAG
HCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCC
DVVELHGNTTYARCIGCGKRHELDWVRQWFRRAGHAPHCTACDEPVKTATISFGQAMPVD
CEEEECCCCEEHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHH
EMRYASQLAQNCDLFLVIGSSLVVWPAAGIPMLAKESGAKLVIINNEPTEQDEIADLVIR
HHHHHHHHHHCCCEEEEECCCEEEECCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHH
HDIGETLGPFVGN
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA