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The map label for this gene is cspE [C]

Identifier: 86750055

GI number: 86750055

Start: 3350686

End: 3351351

Strand: Reverse

Name: cspE [C]

Synonym: RPB_2938

Alternate gene names: 86750055

Gene position: 3351351-3350686 (Counterclockwise)

Preceding gene: 86750056

Following gene: 86750054

Centisome position: 62.86

GC content: 67.12

Gene sequence:

>666_bases
ATGGGGTCGGACGGTTTTGAGTCCAAAAAGCCAGGCGTGATGGCGACCGGCGGTGCCGGGCGGTCAGGGGCCGTTGAATT
CGCGCCTCGCGACGAATTCACGCATCGCGACGGTGTCGGCGCTGCCTTCGCCGGCATCGGCGAAACCGCGAACCTGGTCG
AGATCGGCGGCGTCATCAAATGGTTCGACGCCTCGAAGGGGTACGGCTTCATCGTCCCCGACAACGGCGCTGCGGATGTG
TTGCTGCACGTCACCGTGCTGCGCCGGGACGGCTATCAGACCGCCTATGAGGGCGCACGGATCGTCGTCGAATGCGTCCA
GCGCGCCAAGGGCTATCAGGCGTTCCGGGTCGTCTCGATGGACGAATCCACCGCGATCCATCCGGCCCAGATGCTGCCGC
CGCGCACCCATGTCAGCGTCACGCCGACCAGCGGGCTCGAGCGCGCCCAGGTGAAATGGTTCAACCGGCTGCGCGGTTTC
GGCTTCCTGACCTGCGGAGAGGGCACGCCGGACATCTTCGTACACATGGAGACGCTGCGCCGCTACGGCATGACCGAGCT
GCGCCCCGGCCAGTATGTGCTGGTGCGGTTCGGGCCGGGCTCCAAGGGGATGATGGCGGCGGAGATCCAGCCGGAGAACG
GCGCCCCCGGCCTGTCCTCGCACTGA

Upstream 100 bases:

>100_bases
GCGGCGGCGCAATCCTGATTAGTCATGGAACTGGGGCACGCGGATTGCTGGCGTCGCAGGAGCGGCACGGTTGAAATTTT
GACGCGCGGGGTCCGGGGTC

Downstream 100 bases:

>100_bases
CGGTCCCCGCGTCGGCCTTTCAGACCACGCCGCTCCCCAGATCGCCGATTTGTGATCCGTCGCCGCCTTGGCGCGGCCGT
TCCGTTCATCGCGGTCTGGC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: Transcriptional Regulator Cold Shock Protein; Cold Shock Protein CspA; Cold Shock-Like Protein CspA; Cold Shock Domain-Containing Protein CspD; Cold Shock-Like Protein CspE

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MGSDGFESKKPGVMATGGAGRSGAVEFAPRDEFTHRDGVGAAFAGIGETANLVEIGGVIKWFDASKGYGFIVPDNGAADV
LLHVTVLRRDGYQTAYEGARIVVECVQRAKGYQAFRVVSMDESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRGF
GFLTCGEGTPDIFVHMETLRRYGMTELRPGQYVLVRFGPGSKGMMAAEIQPENGAPGLSSH

Sequences:

>Translated_221_residues
MGSDGFESKKPGVMATGGAGRSGAVEFAPRDEFTHRDGVGAAFAGIGETANLVEIGGVIKWFDASKGYGFIVPDNGAADV
LLHVTVLRRDGYQTAYEGARIVVECVQRAKGYQAFRVVSMDESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRGF
GFLTCGEGTPDIFVHMETLRRYGMTELRPGQYVLVRFGPGSKGMMAAEIQPENGAPGLSSH
>Mature_220_residues
GSDGFESKKPGVMATGGAGRSGAVEFAPRDEFTHRDGVGAAFAGIGETANLVEIGGVIKWFDASKGYGFIVPDNGAADVL
LHVTVLRRDGYQTAYEGARIVVECVQRAKGYQAFRVVSMDESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRGFG
FLTCGEGTPDIFVHMETLRRYGMTELRPGQYVLVRFGPGSKGMMAAEIQPENGAPGLSSH

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=39.1304347826087, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=40.2985074626866, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=38.2352941176471, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23752; Mature: 23621

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDGFESKKPGVMATGGAGRSGAVEFAPRDEFTHRDGVGAAFAGIGETANLVEIGGVIK
CCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCEEECCCEEE
WFDASKGYGFIVPDNGAADVLLHVTVLRRDGYQTAYEGARIVVECVQRAKGYQAFRVVSM
EEECCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRGFGFLTCGEGTPDIFVHMETLR
CCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEHHHHH
RYGMTELRPGQYVLVRFGPGSKGMMAAEIQPENGAPGLSSH
HCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
GSDGFESKKPGVMATGGAGRSGAVEFAPRDEFTHRDGVGAAFAGIGETANLVEIGGVIK
CCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCEEECCCEEE
WFDASKGYGFIVPDNGAADVLLHVTVLRRDGYQTAYEGARIVVECVQRAKGYQAFRVVSM
EEECCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DESTAIHPAQMLPPRTHVSVTPTSGLERAQVKWFNRLRGFGFLTCGEGTPDIFVHMETLR
CCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEHHHHH
RYGMTELRPGQYVLVRFGPGSKGMMAAEIQPENGAPGLSSH
HCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA