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The map label for this gene is ntrC [H]

Identifier: 86749999

GI number: 86749999

Start: 3281606

End: 3283048

Strand: Reverse

Name: ntrC [H]

Synonym: RPB_2882

Alternate gene names: 86749999

Gene position: 3283048-3281606 (Counterclockwise)

Preceding gene: 86750000

Following gene: 86749998

Centisome position: 61.58

GC content: 65.97

Gene sequence:

>1443_bases
ATGCCCGCAGGTAGTATTCTTGTCGCCGATGACGACACCGCCATCCGCACGGTGTTGAACCAGGCGCTGTCGCGCGCCGG
ATACGAAGTGCGGCTGACCGGCAATGCCGCGACGCTGTGGCGCTGGGTCAGCCAGGGCGAGGGCGATCTGGTGATCACCG
ACGTGGTGATGCCGGACGAAAACGCCTTCGACCTGCTGCCGCGGATCAAGAAGATGCGGCCGAACCTGCCGGTCATCGTG
ATGAGCGCGCAGAACACCTTCATGACCGCGATCCGCGCCTCGGAGAAGGGCGCCTACGAATATCTGCCGAAGCCGTTCGA
CCTCAAGGAGCTGATCGCGATCGTCGGCCGCGCGCTGGCCGAGCCGAAGGAGCGCTCCAGCAACCAGAACGACGAGAGCG
AATTCGATTCGATCCCGCTGGTCGGCCGCTCGCCGGCGATGCAGGAAATCTACCGCGTGCTGGCGCGGCTGATGCAGACC
GACCTCACCGTGATGATCTCCGGCGAGAGCGGCACCGGCAAGGAACTGGTGGCGCGCGCGCTGCACGATTACGGCAAGCG
CCGCAACGGCCCGTTCGTCGCGGTCAACATGGCGGCGATCCCGCGCGACCTGATCGAGTCGGAATTGTTCGGCCACGAGC
GCGGCGCCTTTACCGGTGCCAACACCCGCGCCTCCGGCCGGTTCGAACAGGCCGAGGGCGGCACGCTGTTTCTCGACGAG
ATCGGCGACATGCCGATGGAGGCGCAGACGCGGTTGCTGCGGGTGCTGCAGCAGGGCGAATACACCACCGTCGGCGGCCG
CACCCCGATCAAGACCGACGTGCGGATCGTTGCAGCCTCCAACAAGGATTTGCGCATCCTGATCCAGCAGGGTCTGTTCC
GGGAGGATTTGTTCTTTCGTCTCAACGTGGTGCCGCTGCGGCTTCCTCCATTGCGCGAACACATCGAGGATCTGCCGGAC
CTGGTGCGGCACTTCTTCGCGCTGGCGGAGAAGGACGGCCTACCGGCCAAGAAGCTCGACAGCGCGGCGCTGGAGCGGAT
GAAGCAGCATCGCTGGCCGGGCAACGTCCGCGAGCTGGAGAATCTGGCGCGGCGGCTGGCGGCGCTGTATCCGCAGGACG
TCATCACCGCCTCGGTGATCGACGGCGAACTGGCACCGCCCGCGGTGGTGTCCGGCGGCAGCGTCGCCCACACCGTCGAC
AATCTCGGCGGAGCGGTCGAAATGTACCTTTCGTCGCATTTTTCGGGCTTCCCGAACGGCGTCCCGCCGCCGGGGCTCTA
CCATCGCGTGCTGCGCGAGATCGAAGTGCCGCTCTTGACCGCGGCGCTGGCGGCGACTCGCGGCAACCAGATCAGGGCGG
CCGATCTGCTCGGTCTGAACCGCAATACGCTGCGGAAGAAGATCCGCGACCTCGATATTCAGGTTTATCGGACCGGCGGC
TGA

Upstream 100 bases:

>100_bases
GCTGCTGCCGATGTACAACACGGCGAAGAACTACAATCAGAGCAACGGCGAGGACGTGCCGGCGTCATCCGCCTCACAGA
CTGCAAGATGAGGACGAGCA

Downstream 100 bases:

>100_bases
CACGCTGGGTCAAGGTTTTATGCTGTGCACTGCGTAGCCGCCCGAGGTTCTTCGGGCGGCGCGCCGCGATATTGTCGTAA
TTGAGCAACAATGTTGTAGG

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIV
MSAQNTFMTAIRASEKGAYEYLPKPFDLKELIAIVGRALAEPKERSSNQNDESEFDSIPLVGRSPAMQEIYRVLARLMQT
DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLREHIEDLPD
LVRHFFALAEKDGLPAKKLDSAALERMKQHRWPGNVRELENLARRLAALYPQDVITASVIDGELAPPAVVSGGSVAHTVD
NLGGAVEMYLSSHFSGFPNGVPPPGLYHRVLREIEVPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRTGG

Sequences:

>Translated_480_residues
MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIV
MSAQNTFMTAIRASEKGAYEYLPKPFDLKELIAIVGRALAEPKERSSNQNDESEFDSIPLVGRSPAMQEIYRVLARLMQT
DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLREHIEDLPD
LVRHFFALAEKDGLPAKKLDSAALERMKQHRWPGNVRELENLARRLAALYPQDVITASVIDGELAPPAVVSGGSVAHTVD
NLGGAVEMYLSSHFSGFPNGVPPPGLYHRVLREIEVPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRTGG
>Mature_479_residues
PAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIVM
SAQNTFMTAIRASEKGAYEYLPKPFDLKELIAIVGRALAEPKERSSNQNDESEFDSIPLVGRSPAMQEIYRVLARLMQTD
LTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLREHIEDLPDL
VRHFFALAEKDGLPAKKLDSAALERMKQHRWPGNVRELENLARRLAALYPQDVITASVIDGELAPPAVVSGGSVAHTVDN
LGGAVEMYLSSHFSGFPNGVPPPGLYHRVLREIEVPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRTGG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=479, Percent_Identity=45.3027139874739, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1788550, Length=477, Percent_Identity=37.5262054507338, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=38.412017167382, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788905, Length=462, Percent_Identity=35.9307359307359, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1789233, Length=349, Percent_Identity=40.1146131805158, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=345, Percent_Identity=42.0289855072464, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=49.3506493506493, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=254, Percent_Identity=44.0944881889764, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=44.8, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081872, Length=342, Percent_Identity=37.719298245614, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1787583, Length=253, Percent_Identity=40.3162055335968, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789828, Length=289, Percent_Identity=30.7958477508651, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=35.7843137254902, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=32.1100917431193, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790552, Length=131, Percent_Identity=34.3511450381679, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786784, Length=109, Percent_Identity=29.3577981651376, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52940; Mature: 52808

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDE
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIRASEKGAYEYLPKPFDLKELIAIVGRALA
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKERSSNQNDESEFDSIPLVGRSPAMQEIYRVLARLMQTDLTVMISGESGTGKELVARA
CCHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
HHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCEEHHHHHCCHHHCCEEE
LNVVPLRLPPLREHIEDLPDLVRHFFALAEKDGLPAKKLDSAALERMKQHRWPGNVRELE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
NLARRLAALYPQDVITASVIDGELAPPAVVSGGSVAHTVDNLGGAVEMYLSSHFSGFPNG
HHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCC
VPPPGLYHRVLREIEVPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRTGG
CCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
PAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVSQGEGDLVITDVVMPDE
CCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIRASEKGAYEYLPKPFDLKELIAIVGRALA
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKERSSNQNDESEFDSIPLVGRSPAMQEIYRVLARLMQTDLTVMISGESGTGKELVARA
CCHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFTGANTRASGRFEQAEGGTLFLDE
HHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAASNKDLRILIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCEEHHHHHCCHHHCCEEE
LNVVPLRLPPLREHIEDLPDLVRHFFALAEKDGLPAKKLDSAALERMKQHRWPGNVRELE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
NLARRLAALYPQDVITASVIDGELAPPAVVSGGSVAHTVDNLGGAVEMYLSSHFSGFPNG
HHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCC
VPPPGLYHRVLREIEVPLLTAALAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRTGG
CCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]