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The map label for this gene is ntrB [H]

Identifier: 86750000

GI number: 86750000

Start: 3283058

End: 3284227

Strand: Reverse

Name: ntrB [H]

Synonym: RPB_2883

Alternate gene names: 86750000

Gene position: 3284227-3283058 (Counterclockwise)

Preceding gene: 86750001

Following gene: 86749999

Centisome position: 61.6

GC content: 64.96

Gene sequence:

>1170_bases
ATGAACGCCGTCCTCGAACCGCGCCACGCCAAGCCGACCGAAAGCGATGCGATCCTCAACGCGCTGCCGAATCCCGTGCT
GTTGATCGGCCCGGACGGCAAGATCATCGACGCCAACATGGCGGCGGAATCGTTCTTCGAGATTTCGACGCAGCTCCTTC
GGCGCCAATCGCTGACCGAGCTGGTGCCGTTCGGCAGCCCGCTGCTGGCGTTGATCGAGCAGGTGCGTAGCGGCAATTCG
CCGGTCAACGAGTACAAGGTCGATCTCGGCACGCCGCGGATCGGCGCCGACCGTCAGGTCGATCTGCATGTTGCGCCGCT
GAACGAACGCCCCGGCCACATCGTGGTGATGCTGCAGGAGCGCACCATCGCCGACAAGATGGACCGGCAGCTCACCCATC
GCAGCGCCGCGCGTTCGGTGATCGCGCTGGCGGCGATGCTGGCGCACGAGATCAAGAACCCGCTGTCCGGCATCCGCGGC
GCGGCTCAGCTCCTCGAGCAGCAGGCGTCGTCGGAAGACCGGATGCTGACGCGGCTGATCTGCGACGAGGCCGATCGGAT
CGTCACGCTGGTCGATCGGATGGAAGTTTTCGGCGACGACCGCCCGGTGGCGCGCGGCCCGGTCAACATCCATTCGGTGC
TCGATCACGTCAAACGGCTCGCGCAGTCCGGCTTCGCGCGCAACATCAAGTTCGTCGAGGACTACGACCCGTCGCTGCCG
CCGGTGCTCGCCAATCAGGATCAACTGATCCAGGTGTTTCTGAATCTGGTGAAGAACGCCGCCGAGGCCGTGATCGATCT
CGGCAGCGACGCCGAGATCCAGCTCACCACCGCGTTCCGTCCCGGCGTGCGGCTGTCGGTGCCCGGCAAGAAGACCCGGG
TGTCGCTGCCGCTGGAATTCTGCGTCAAGGACAACGGGCCCGGCGTGCCGGAAGACCTGATGCCGAACCTGTTCGATCCG
TTCGTCACCACCAAGGCGTCGGGCTCAGGCCTCGGCCTCGCGCTCGTCGCCAAGATCGTCGGCGACCACGGCGGAATCAT
CGAATGTGAATCGCAGCCGCGCAAGACCACGTTTCGCGTGCTGCTGCCGATGTACAACACGGCGAAGAACTACAATCAGA
GCAACGGCGAGGACGTGCCGGCGTCATCCGCCTCACAGACTGCAAGATGA

Upstream 100 bases:

>100_bases
TGAAGCGGTGGAGGACGACGATCCTCACCGACGACAGCCCGACCCGTGTGCATCATGCGCTCACTGACGCCTACGACGAT
TTCGCCTGGAGAGCCGCCGC

Downstream 100 bases:

>100_bases
GGACGAGCAATGCCCGCAGGTAGTATTCTTGTCGCCGATGACGACACCGCCATCCGCACGGTGTTGAACCAGGCGCTGTC
GCGCGCCGGATACGAAGTGC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNAVLEPRHAKPTESDAILNALPNPVLLIGPDGKIIDANMAAESFFEISTQLLRRQSLTELVPFGSPLLALIEQVRSGNS
PVNEYKVDLGTPRIGADRQVDLHVAPLNERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIKFVEDYDPSLP
PVLANQDQLIQVFLNLVKNAAEAVIDLGSDAEIQLTTAFRPGVRLSVPGKKTRVSLPLEFCVKDNGPGVPEDLMPNLFDP
FVTTKASGSGLGLALVAKIVGDHGGIIECESQPRKTTFRVLLPMYNTAKNYNQSNGEDVPASSASQTAR

Sequences:

>Translated_389_residues
MNAVLEPRHAKPTESDAILNALPNPVLLIGPDGKIIDANMAAESFFEISTQLLRRQSLTELVPFGSPLLALIEQVRSGNS
PVNEYKVDLGTPRIGADRQVDLHVAPLNERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIKFVEDYDPSLP
PVLANQDQLIQVFLNLVKNAAEAVIDLGSDAEIQLTTAFRPGVRLSVPGKKTRVSLPLEFCVKDNGPGVPEDLMPNLFDP
FVTTKASGSGLGLALVAKIVGDHGGIIECESQPRKTTFRVLLPMYNTAKNYNQSNGEDVPASSASQTAR
>Mature_389_residues
MNAVLEPRHAKPTESDAILNALPNPVLLIGPDGKIIDANMAAESFFEISTQLLRRQSLTELVPFGSPLLALIEQVRSGNS
PVNEYKVDLGTPRIGADRQVDLHVAPLNERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIKFVEDYDPSLP
PVLANQDQLIQVFLNLVKNAAEAVIDLGSDAEIQLTTAFRPGVRLSVPGKKTRVSLPLEFCVKDNGPGVPEDLMPNLFDP
FVTTKASGSGLGLALVAKIVGDHGGIIECESQPRKTTFRVLLPMYNTAKNYNQSNGEDVPASSASQTAR

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=368, Percent_Identity=33.9673913043478, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=34.4827586206897, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788549, Length=363, Percent_Identity=27.8236914600551, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786600, Length=365, Percent_Identity=24.1095890410959, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789808, Length=277, Percent_Identity=26.3537906137184, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42329; Mature: 42329

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVLEPRHAKPTESDAILNALPNPVLLIGPDGKIIDANMAAESFFEISTQLLRRQSLTE
CCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LVPFGSPLLALIEQVRSGNSPVNEYKVDLGTPRIGADRQVDLHVAPLNERPGHIVVMLQE
HHCCCCHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEECC
RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQQASSEDRMLTRLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
CDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIKFVEDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCC
PVLANQDQLIQVFLNLVKNAAEAVIDLGSDAEIQLTTAFRPGVRLSVPGKKTRVSLPLEF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEECCCCCEEEECCCEE
CVKDNGPGVPEDLMPNLFDPFVTTKASGSGLGLALVAKIVGDHGGIIECESQPRKTTFRV
EEECCCCCCCHHHCHHHHCCHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEE
LLPMYNTAKNYNQSNGEDVPASSASQTAR
EEHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAVLEPRHAKPTESDAILNALPNPVLLIGPDGKIIDANMAAESFFEISTQLLRRQSLTE
CCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LVPFGSPLLALIEQVRSGNSPVNEYKVDLGTPRIGADRQVDLHVAPLNERPGHIVVMLQE
HHCCCCHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEECC
RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQQASSEDRMLTRLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
CDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKRLAQSGFARNIKFVEDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCC
PVLANQDQLIQVFLNLVKNAAEAVIDLGSDAEIQLTTAFRPGVRLSVPGKKTRVSLPLEF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEECCCCCEEEECCCEE
CVKDNGPGVPEDLMPNLFDPFVTTKASGSGLGLALVAKIVGDHGGIIECESQPRKTTFRV
EEECCCCCCCHHHCHHHHCCHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEE
LLPMYNTAKNYNQSNGEDVPASSASQTAR
EEHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]