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The map label for this gene is glmU

Identifier: 86749972

GI number: 86749972

Start: 3254379

End: 3255737

Strand: Reverse

Name: glmU

Synonym: RPB_2855

Alternate gene names: 86749972

Gene position: 3255737-3254379 (Counterclockwise)

Preceding gene: 86749975

Following gene: 86749971

Centisome position: 61.06

GC content: 66.74

Gene sequence:

>1359_bases
ATGACGACGAGAACCAGCCTGACGATCGTACTTGCTGCCGGAGAGGGCACCCGGATGCGATCGTCGCTACCAAAGGTGCT
TCACCCGGTGGCCGGCCGCCCGCTGTTGGCTCATGTGCTGGCCGCAGCACCGCATGGCGAAGGCGACAAACTCGCCGTGG
TGATTGGCCCCGATCATCAGGCGGTCGCCGACGAGGCCAGACGAATCCGACCCGATGCGCAGATCTACGTGCAGACGGAG
CGTCTCGGCACCGCGCACGCCGTACTGGCGGCGAAGCAGGCGGTCGCGGGCGGCGCCGACGATCTGCTGATCGCCTTCGG
CGACACCCCGCTGATTTCGGCGGAGACCTTCGCCCGCCTGCGCGAACCGCTGCGCAATGGCGGCGCGCTGGTGGTGCTCG
GCTTCCGGGCGGTCGATCCGACCGGTTACGGCCGGCTGGTCGTCGAGGACGATCGATTGGCCGCGATCCGCGAGCAGGCC
GATGCCAGCCCGGACGAACTGAAGATCACGCTGTGCAATGCCGGCGTGATGGCGATCGACGGGCGCATCGCGCTCGACGT
GCTGGGCGCGATCGGCAACGCCAACAAGAAGGGCGAGTACTATCTGACTGACGCGGTCGGCATCGTGCGCGAACGCGGCC
TCGTCGCCGGCGTGATCGAGACCGACGAGGACGAGGTCCGCGGCATCAACACAAAGGCGCAACTCGCCGAGGCCGAAGCG
GTGATGCAGGCGCGGCTGCGCCAGGCCGCGATGGCCGCGGGTGTGACGCTGATCTCGCCGGAGACGATTCACCTCGCCGC
CGATACCACGTTCGGCCGGGACGTGACGATCGAACCATTCGTGGTGATCGGCCCCGGCGTCAGCATCGGCGACGGTGCGG
TGATCCATTCGTTCTCGCACATCGTCGACACCTCGCTCGGCAAGAATACATCGATCGGCCCCTATGCGCGGCTGCGGCCC
GGCACGTCGCTCGGCGACGGCGCCAAGATCGGCAATTTCGTCGAGACCAAAGCAGCGCAGATCGATGCCGGCGCCAAGGT
CAATCATCTGACCTATATCGGCGACGCGCATATCGGCCCGGGCGCCAATATCGGCGCCGGAACGATCACCTGCAATTACG
ACGGCTTCAACAAGCACAAGACCGAAATCGGCGCCGGCGCCTTCGTCGGCTCGAACTCGTCGCTGGTCGCGCCGGTCAGG
ATCGGCGCGGGCGCGTATATCGGTTCGGGATCGGTGATTACCAGGAACGTGCCGGACGACGCGCTGGCGGTCGAACGCAA
CGACCAGAGTGTGCGCGAGGGCTGGGCAACGCGGTTCCGTGAAGCCAAGCTGCGCGCGAAGAAACCCAAAGCCGGCTGA

Upstream 100 bases:

>100_bases
TCCACGCAGCCGGCCCGGCATTTTCGTTCCCCCCAAAGCGGTTTCCCCTCGGACTGAAAACCCTTTATGAGGGCTGCAGT
TCGACCGCGTGGGATTGCAG

Downstream 100 bases:

>100_bases
CGTTTCGGCTGCCGCGATTGGTTAATGCTGCGTCGCTTAACCCTGTCGCAATCCGCAATAATTATTGACTACCTCGGCTT
CGGCGGAACTCGCTAGATGT

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTTRTSLTIVLAAGEGTRMRSSLPKVLHPVAGRPLLAHVLAAAPHGEGDKLAVVIGPDHQAVADEARRIRPDAQIYVQTE
RLGTAHAVLAAKQAVAGGADDLLIAFGDTPLISAETFARLREPLRNGGALVVLGFRAVDPTGYGRLVVEDDRLAAIREQA
DASPDELKITLCNAGVMAIDGRIALDVLGAIGNANKKGEYYLTDAVGIVRERGLVAGVIETDEDEVRGINTKAQLAEAEA
VMQARLRQAAMAAGVTLISPETIHLAADTTFGRDVTIEPFVVIGPGVSIGDGAVIHSFSHIVDTSLGKNTSIGPYARLRP
GTSLGDGAKIGNFVETKAAQIDAGAKVNHLTYIGDAHIGPGANIGAGTITCNYDGFNKHKTEIGAGAFVGSNSSLVAPVR
IGAGAYIGSGSVITRNVPDDALAVERNDQSVREGWATRFREAKLRAKKPKAG

Sequences:

>Translated_452_residues
MTTRTSLTIVLAAGEGTRMRSSLPKVLHPVAGRPLLAHVLAAAPHGEGDKLAVVIGPDHQAVADEARRIRPDAQIYVQTE
RLGTAHAVLAAKQAVAGGADDLLIAFGDTPLISAETFARLREPLRNGGALVVLGFRAVDPTGYGRLVVEDDRLAAIREQA
DASPDELKITLCNAGVMAIDGRIALDVLGAIGNANKKGEYYLTDAVGIVRERGLVAGVIETDEDEVRGINTKAQLAEAEA
VMQARLRQAAMAAGVTLISPETIHLAADTTFGRDVTIEPFVVIGPGVSIGDGAVIHSFSHIVDTSLGKNTSIGPYARLRP
GTSLGDGAKIGNFVETKAAQIDAGAKVNHLTYIGDAHIGPGANIGAGTITCNYDGFNKHKTEIGAGAFVGSNSSLVAPVR
IGAGAYIGSGSVITRNVPDDALAVERNDQSVREGWATRFREAKLRAKKPKAG
>Mature_451_residues
TTRTSLTIVLAAGEGTRMRSSLPKVLHPVAGRPLLAHVLAAAPHGEGDKLAVVIGPDHQAVADEARRIRPDAQIYVQTER
LGTAHAVLAAKQAVAGGADDLLIAFGDTPLISAETFARLREPLRNGGALVVLGFRAVDPTGYGRLVVEDDRLAAIREQAD
ASPDELKITLCNAGVMAIDGRIALDVLGAIGNANKKGEYYLTDAVGIVRERGLVAGVIETDEDEVRGINTKAQLAEAEAV
MQARLRQAAMAAGVTLISPETIHLAADTTFGRDVTIEPFVVIGPGVSIGDGAVIHSFSHIVDTSLGKNTSIGPYARLRPG
TSLGDGAKIGNFVETKAAQIDAGAKVNHLTYIGDAHIGPGANIGAGTITCNYDGFNKHKTEIGAGAFVGSNSSLVAPVRI
GAGAYIGSGSVITRNVPDDALAVERNDQSVREGWATRFREAKLRAKKPKAG

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=459, Percent_Identity=40.958605664488, Blast_Score=311, Evalue=4e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_RHOP2 (Q2IW53)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486468.1
- ProteinModelPortal:   Q2IW53
- SMR:   Q2IW53
- STRING:   Q2IW53
- GeneID:   3910648
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2855
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14353
- BioCyc:   RPAL316058:RPB_2855-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR001228
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF01128 IspD; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 47079; Mature: 46948

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 349-349 BINDING 78-78 BINDING 144-144 BINDING 158-158 BINDING 173-173 BINDING 373-373 BINDING 391-391 BINDING 426-426

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRTSLTIVLAAGEGTRMRSSLPKVLHPVAGRPLLAHVLAAAPHGEGDKLAVVIGPDHQ
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCH
AVADEARRIRPDAQIYVQTERLGTAHAVLAAKQAVAGGADDLLIAFGDTPLISAETFARL
HHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
REPLRNGGALVVLGFRAVDPTGYGRLVVEDDRLAAIREQADASPDELKITLCNAGVMAID
HHHHHCCCEEEEEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCEEEEC
GRIALDVLGAIGNANKKGEYYLTDAVGIVRERGLVAGVIETDEDEVRGINTKAQLAEAEA
CEEEEHHHHHHCCCCCCCCEEEEHHHHHHHHCCEEEEEEECCHHHHCCCCCHHHHHHHHH
VMQARLRQAAMAAGVTLISPETIHLAADTTFGRDVTIEPFVVIGPGVSIGDGAVIHSFSH
HHHHHHHHHHHHCCEEEECCCEEEEEECCCCCCCEEECCEEEECCCCCCCCCHHHHHHHH
IVDTSLGKNTSIGPYARLRPGTSLGDGAKIGNFVETKAAQIDAGAKVNHLTYIGDAHIGP
HHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHEECCCCEEEEEEEECCCCCCC
GANIGAGTITCNYDGFNKHKTEIGAGAFVGSNSSLVAPVRIGAGAYIGSGSVITRNVPDD
CCCCCCCEEEEECCCCCCCHHHCCCCEEECCCCCEEEEEEECCCEEECCCCEEEECCCCC
ALAVERNDQSVREGWATRFREAKLRAKKPKAG
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTRTSLTIVLAAGEGTRMRSSLPKVLHPVAGRPLLAHVLAAAPHGEGDKLAVVIGPDHQ
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCH
AVADEARRIRPDAQIYVQTERLGTAHAVLAAKQAVAGGADDLLIAFGDTPLISAETFARL
HHHHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
REPLRNGGALVVLGFRAVDPTGYGRLVVEDDRLAAIREQADASPDELKITLCNAGVMAID
HHHHHCCCEEEEEEEEEECCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCEEEEC
GRIALDVLGAIGNANKKGEYYLTDAVGIVRERGLVAGVIETDEDEVRGINTKAQLAEAEA
CEEEEHHHHHHCCCCCCCCEEEEHHHHHHHHCCEEEEEEECCHHHHCCCCCHHHHHHHHH
VMQARLRQAAMAAGVTLISPETIHLAADTTFGRDVTIEPFVVIGPGVSIGDGAVIHSFSH
HHHHHHHHHHHHCCEEEECCCEEEEEECCCCCCCEEECCEEEECCCCCCCCCHHHHHHHH
IVDTSLGKNTSIGPYARLRPGTSLGDGAKIGNFVETKAAQIDAGAKVNHLTYIGDAHIGP
HHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHEECCCCEEEEEEEECCCCCCC
GANIGAGTITCNYDGFNKHKTEIGAGAFVGSNSSLVAPVRIGAGAYIGSGSVITRNVPDD
CCCCCCCEEEEECCCCCCCHHHCCCCEEECCCCCEEEEEEECCCEEECCCCEEEECCCCC
ALAVERNDQSVREGWATRFREAKLRAKKPKAG
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA