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The map label for this gene is tsf

Identifier: 86749944

GI number: 86749944

Start: 3221206

End: 3222132

Strand: Reverse

Name: tsf

Synonym: RPB_2827

Alternate gene names: 86749944

Gene position: 3222132-3221206 (Counterclockwise)

Preceding gene: 86749945

Following gene: 86749943

Centisome position: 60.43

GC content: 65.8

Gene sequence:

>927_bases
ATGGCAACGATTACTGCAGCGATGGTCAAGGAACTGCGCGAGACCACCGGCGTCGGCATGATGGACTGCAAGCAGGCGCT
CGCCGAGAACGACGGCAACATGGAGGCCGCGGTCGACTGGCTGCGCAAGAAGGGTTTGTCGAAGGCCGCCAAGAAGGCCG
GCCGCGTCGCCGCGGAAGGCCTGATCGGTGCGCTGACCGACGGCACCAAGGGCGTCGTCATCGAAGTCAATTCGGAGACC
GATTTCGTCGCGCGCAACGAGCAGTTCCAGGGTCTGGTCAAGATGATCGCCCAGGTCGCGCTCAAGGTCGGCGCCGATGT
CGACAAGATCAACGCGGCCCCGGTCGGCTCCTCGACCGTCGCCGGTGCGATCTCGGATGCGATCGCCACGATCGGCGAGA
ACATGACCCTTCGCCGCGCCGCTGCGCTGGAAGTGTCGCAGGGCGTGGTCGCGAGCTACGTCCACGGTGCGGTGATCGAG
GGCGCCGGCAAGATGGGCGTGATCGTCGCGCTGGAATCCACCGGCAAGACCGACGAGCTCGCCGCGCTCGGCCGCCAGCT
CGCGATGCACGTCGCCGCCGCCAATCCGCAGGCGCTGGATCCGGCCGGGCTCGATCCGGACGTGGTTCGCCGCGAGCGCG
AGGTGATGGCCGACAAATATCGCCAGCAGGGCAAGCCGGAAAACATGATCGAGAAGATCGTCGAGAACGGCCTGAAGACC
TACTACAAGGAAGTCTGTCTGCTGGAGCAGGCCTACATCCACGACGAGAAGGGCAAGGCCGTCGGCCAGGCGGTGAAGGA
AGCCGAGGGCAAGGTCGGCGCGCCGATCAAGATCATCGGCTTCGTCCGCTACGCGCTCGGCGAAGGCATCGAGAAGCAGA
CCTCGGACTTCGCGGCGGAAGTCGCCGCGGTCTCGGGCCAGAAGTAA

Upstream 100 bases:

>100_bases
GGCCGCAACGTTCCGTCGTCAAAATCTGCAATGAAATTCCTGATCGCGTGCCGCGGGCTCTTGCCACGCGATGCGCCCTG
ACAGGCAAAGGACTACGAGC

Downstream 100 bases:

>100_bases
TTGGGTCAGAAGTAATCGCTTTTCACGTGATCAACTCCCGGCCGTTCCGGCCGGGAGCGAACCCACCGAGGACGCGACCG
CATCATGGGTGAGCCGATCT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MATITAAMVKELRETTGVGMMDCKQALAENDGNMEAAVDWLRKKGLSKAAKKAGRVAAEGLIGALTDGTKGVVIEVNSET
DFVARNEQFQGLVKMIAQVALKVGADVDKINAAPVGSSTVAGAISDAIATIGENMTLRRAAALEVSQGVVASYVHGAVIE
GAGKMGVIVALESTGKTDELAALGRQLAMHVAAANPQALDPAGLDPDVVRREREVMADKYRQQGKPENMIEKIVENGLKT
YYKEVCLLEQAYIHDEKGKAVGQAVKEAEGKVGAPIKIIGFVRYALGEGIEKQTSDFAAEVAAVSGQK

Sequences:

>Translated_308_residues
MATITAAMVKELRETTGVGMMDCKQALAENDGNMEAAVDWLRKKGLSKAAKKAGRVAAEGLIGALTDGTKGVVIEVNSET
DFVARNEQFQGLVKMIAQVALKVGADVDKINAAPVGSSTVAGAISDAIATIGENMTLRRAAALEVSQGVVASYVHGAVIE
GAGKMGVIVALESTGKTDELAALGRQLAMHVAAANPQALDPAGLDPDVVRREREVMADKYRQQGKPENMIEKIVENGLKT
YYKEVCLLEQAYIHDEKGKAVGQAVKEAEGKVGAPIKIIGFVRYALGEGIEKQTSDFAAEVAAVSGQK
>Mature_307_residues
ATITAAMVKELRETTGVGMMDCKQALAENDGNMEAAVDWLRKKGLSKAAKKAGRVAAEGLIGALTDGTKGVVIEVNSETD
FVARNEQFQGLVKMIAQVALKVGADVDKINAAPVGSSTVAGAISDAIATIGENMTLRRAAALEVSQGVVASYVHGAVIEG
AGKMGVIVALESTGKTDELAALGRQLAMHVAAANPQALDPAGLDPDVVRREREVMADKYRQQGKPENMIEKIVENGLKTY
YKEVCLLEQAYIHDEKGKAVGQAVKEAEGKVGAPIKIIGFVRYALGEGIEKQTSDFAAEVAAVSGQK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=220, Percent_Identity=37.7272727272727, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI291084500, Length=241, Percent_Identity=34.4398340248963, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI291084502, Length=94, Percent_Identity=46.8085106382979, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI291084498, Length=94, Percent_Identity=46.8085106382979, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786366, Length=309, Percent_Identity=44.9838187702265, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17561440, Length=304, Percent_Identity=28.2894736842105, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921466, Length=308, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_RHOP2 (Q2IW81)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486440.1
- ProteinModelPortal:   Q2IW81
- SMR:   Q2IW81
- STRING:   Q2IW81
- GeneID:   3910620
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2827
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   RPAL316058:RPB_2827-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 32324; Mature: 32192

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATITAAMVKELRETTGVGMMDCKQALAENDGNMEAAVDWLRKKGLSKAAKKAGRVAAEG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHH
LIGALTDGTKGVVIEVNSETDFVARNEQFQGLVKMIAQVALKVGADVDKINAAPVGSSTV
HHHHCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHH
AGAISDAIATIGENMTLRRAAALEVSQGVVASYVHGAVIEGAGKMGVIVALESTGKTDEL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
AALGRQLAMHVAAANPQALDPAGLDPDVVRREREVMADKYRQQGKPENMIEKIVENGLKT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YYKEVCLLEQAYIHDEKGKAVGQAVKEAEGKVGAPIKIIGFVRYALGEGIEKQTSDFAAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VAAVSGQK
HHHHCCCC
>Mature Secondary Structure 
ATITAAMVKELRETTGVGMMDCKQALAENDGNMEAAVDWLRKKGLSKAAKKAGRVAAEG
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHH
LIGALTDGTKGVVIEVNSETDFVARNEQFQGLVKMIAQVALKVGADVDKINAAPVGSSTV
HHHHCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHH
AGAISDAIATIGENMTLRRAAALEVSQGVVASYVHGAVIEGAGKMGVIVALESTGKTDEL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
AALGRQLAMHVAAANPQALDPAGLDPDVVRREREVMADKYRQQGKPENMIEKIVENGLKT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YYKEVCLLEQAYIHDEKGKAVGQAVKEAEGKVGAPIKIIGFVRYALGEGIEKQTSDFAAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VAAVSGQK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA