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The map label for this gene is paaF [C]

Identifier: 86749820

GI number: 86749820

Start: 3088732

End: 3089538

Strand: Reverse

Name: paaF [C]

Synonym: RPB_2703

Alternate gene names: 86749820

Gene position: 3089538-3088732 (Counterclockwise)

Preceding gene: 86749825

Following gene: 86749819

Centisome position: 57.95

GC content: 68.9

Gene sequence:

>807_bases
GTGGGGCAGTTCGTGACAATCGAGCGGGGGCTCGGGCCGGAGGGCCGTGTCGCCGTGGTGCGGTTCGACCGCGGCGACGG
CATCAACGCGCTGTCGCCGGACGCGATGCGGCAATTGACCGACGCCGCGCGCAGCTTTGAAGACGATGGCGCCACCTCGG
TGGTGGTACTTGCGGGCAACGCTATGGCATTCAGCGCCGGCTTCGATCTCAAGGACCCGGAAGGACGGGGCCGCGCCGGG
ATGGATCTCGGCGCATTGCGCCGGCACCTCAAGCTCGGCCCGCGGCTGACGCGCGCCTGGCAGGAGATGGAACAGATCAC
CATCGGCGCGATCGACGGCTTCTGCATCGGCGGCGGCGTCGCGCTGGCGGTGGCGCTGGATTTCCGGGTGATGGCGCGCG
ACGCGCATATGCGCGTGCCGGAAATCGGCCTCGGCATGAACATGAGCTGGCAGAGCGTGCCGCGGATGCTGCATCTGATC
GGCCCGGCGCGCACCAAGCAGGCGGTGATCCTCGCCGACGACCGGATCGGTGCCGACGACGCCTACGAATGGGGCCTGGT
CGAGCATCTGGCCGAGCCGGGGCAGGCGTTCACCGCAGCGATGGCGCTGGCCGACAAGGTCGCGGCGCAGCCGCCGATGT
CGGTGGCGATGACCAAGCTCACCGTCAACCGGCTGGCGCACGCGCTGGACGATCTCGCCAGCCACATGGACCTCGACCAG
TTCGCACTCGCGGGCATGAGCGAGGACCACCGGGAAGGCGTCGCCGCATTTTTGCATCGCCGCAAGCCCACTTTTCGCGG
AAAGTGA

Upstream 100 bases:

>100_bases
TGTTCGCTCATGCGACGCAACATAGATTAGAAAATGTTGCGCTGTCGAGCCGGGAAGCTGCTAGCCTTCGGACAACCAAG
AATGCAGTTCGGGAGGCATC

Downstream 100 bases:

>100_bases
GCAGCGTCGGCGCGGCTGCCGCGTTATCATGCGTTCAAACCAATCGGGAGGTGTCGATGATCACGACCATCGTGCAGTTC
GCGCTGTCGGAGCCGATCAG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MGQFVTIERGLGPEGRVAVVRFDRGDGINALSPDAMRQLTDAARSFEDDGATSVVVLAGNAMAFSAGFDLKDPEGRGRAG
MDLGALRRHLKLGPRLTRAWQEMEQITIGAIDGFCIGGGVALAVALDFRVMARDAHMRVPEIGLGMNMSWQSVPRMLHLI
GPARTKQAVILADDRIGADDAYEWGLVEHLAEPGQAFTAAMALADKVAAQPPMSVAMTKLTVNRLAHALDDLASHMDLDQ
FALAGMSEDHREGVAAFLHRRKPTFRGK

Sequences:

>Translated_268_residues
MGQFVTIERGLGPEGRVAVVRFDRGDGINALSPDAMRQLTDAARSFEDDGATSVVVLAGNAMAFSAGFDLKDPEGRGRAG
MDLGALRRHLKLGPRLTRAWQEMEQITIGAIDGFCIGGGVALAVALDFRVMARDAHMRVPEIGLGMNMSWQSVPRMLHLI
GPARTKQAVILADDRIGADDAYEWGLVEHLAEPGQAFTAAMALADKVAAQPPMSVAMTKLTVNRLAHALDDLASHMDLDQ
FALAGMSEDHREGVAAFLHRRKPTFRGK
>Mature_267_residues
GQFVTIERGLGPEGRVAVVRFDRGDGINALSPDAMRQLTDAARSFEDDGATSVVVLAGNAMAFSAGFDLKDPEGRGRAGM
DLGALRRHLKLGPRLTRAWQEMEQITIGAIDGFCIGGGVALAVALDFRVMARDAHMRVPEIGLGMNMSWQSVPRMLHLIG
PARTKQAVILADDRIGADDAYEWGLVEHLAEPGQAFTAAMALADKVAAQPPMSVAMTKLTVNRLAHALDDLASHMDLDQF
ALAGMSEDHREGVAAFLHRRKPTFRGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=27.1317829457364, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=26.8722466960352, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4502327, Length=274, Percent_Identity=27.7372262773723, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=27.843137254902, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=26.2357414448669, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=28.0769230769231, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=266, Percent_Identity=27.0676691729323, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=270, Percent_Identity=28.5185185185185, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=252, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=24.4274809160305, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=23.6842105263158, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=244, Percent_Identity=24.5901639344262, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653139, Length=274, Percent_Identity=28.1021897810219, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=25.8555133079848, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=25.8555133079848, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24650670, Length=245, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920382, Length=274, Percent_Identity=25.9124087591241, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357171, Length=251, Percent_Identity=25.8964143426295, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28870; Mature: 28738

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQFVTIERGLGPEGRVAVVRFDRGDGINALSPDAMRQLTDAARSFEDDGATSVVVLAGN
CCCEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
AMAFSAGFDLKDPEGRGRAGMDLGALRRHLKLGPRLTRAWQEMEQITIGAIDGFCIGGGV
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCHH
ALAVALDFRVMARDAHMRVPEIGLGMNMSWQSVPRMLHLIGPARTKQAVILADDRIGADD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
AYEWGLVEHLAEPGQAFTAAMALADKVAAQPPMSVAMTKLTVNRLAHALDDLASHMDLDQ
CHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FALAGMSEDHREGVAAFLHRRKPTFRGK
HHHCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GQFVTIERGLGPEGRVAVVRFDRGDGINALSPDAMRQLTDAARSFEDDGATSVVVLAGN
CCEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
AMAFSAGFDLKDPEGRGRAGMDLGALRRHLKLGPRLTRAWQEMEQITIGAIDGFCIGGGV
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCHH
ALAVALDFRVMARDAHMRVPEIGLGMNMSWQSVPRMLHLIGPARTKQAVILADDRIGADD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
AYEWGLVEHLAEPGQAFTAAMALADKVAAQPPMSVAMTKLTVNRLAHALDDLASHMDLDQ
CHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FALAGMSEDHREGVAAFLHRRKPTFRGK
HHHCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA