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The map label for this gene is bchE [H]

Identifier: 86749804

GI number: 86749804

Start: 3071354

End: 3072880

Strand: Reverse

Name: bchE [H]

Synonym: RPB_2687

Alternate gene names: 86749804

Gene position: 3072880-3071354 (Counterclockwise)

Preceding gene: 86749805

Following gene: 86749803

Centisome position: 57.63

GC content: 65.62

Gene sequence:

>1527_bases
ATGCGTATCCTTCTGGTCAACGTTCCCCATCCCGCCATCGGCAGCCTGATTCCGAGCGATCACTTGCCGCCGCTGGGCCT
GCTGGCGATCGGCGGACCGCTGATTGATGACGGCCATGACGTGCGTTTGCTTGATGCCGAGTTCGGCCCGACGTCGACCG
CGCAGATCGTCGGACAGGCGCGCGACTTCCGTCCCGATGCGGTGCTGTTCGGCCATTCCGGGTCGACCTCCGGCCACCCG
GTCATCGCCGAAGTCGCACAGGCCATCGCGCATGCCATTCCCGGCACGCGCATTGTCTATGGAGGTGTGTTTCCGACCTA
CCACTGGCGGGAGATCCTCGACGCCGAGCCTTACGTCACGGCCATCGTGCGTGGGGAGGGCGAGGAGACGGCGCGGCGCT
TGATGACCGCACTCGCTGATGGCGATGATCTCGCGGGCGTCCATGGGATTGCCTATCGCAGAGCCGGGCAAGCCTGCGCG
ACGCCGCCGGCCGTGGTGATCGGAGATCTCGACGCCTATCGGATCGGTTGGGAACTGATCGACCACGCTCGCTACAGCTA
TTGGGGCGGACTGCGCGCCGTCGTGGTGCAATTCTCGCGAGGCTGCCCACATCTGTGCAGTTACTGCGGACAACGCGGCT
TCTGGACGCGCTGGCGGCACCGCGATCCCGTGCTGTTCGCCAAGGAGCTCGCGCGGCTGCATCGGGAGCAGGGCGTCCGG
GTCGTCAATTTCGCCGACGAGAACCCGACGGTCTCGAAGAAGGTGTGGCAGACGTTCCTCGAGGCGTTGATCGCGGAGGA
GGTCGACCTGATCCTGGTGGGGTCGACCAGGGCCGACGACATCGTCCGCGACGCCAATATCTTGCATCTGTACAAGCAGG
CCGGCTGGGATCGCTTCCTGCTCGGCCTGGAAAACACCGACGACGCCACGCTGGCGCTGATCCGCAAGGGCGCGGCAACG
CCCACCGATCGCGAGGCCATTCGGCTGCTGCGTCGGCACGGCATCCTATCGATGGCCACCTGGGTGGTCGGCTTCGTCGA
GGAGACCGACCGCGATCACTGGCGCGGGCTGCGCCAGCTTCTCTCGTACGACCCGGACCAGATTCAGATGCTGTACGCGA
CGCCGCACCGCTGGACGCCATATTTCGGGCAGGCGGCCGAACGCCGGGTGATCACGACTGACCGGCGGCACTGGGACTAC
AAGCATCAGGTCCTCGCCAATCGCAACATGCCGCCGTGGCGCGTCCTGCTCTGGTTCAAGTTCACCGAGCTGGTGCTTCA
AGCCCGCCCGAAGGCGATGTTTCGCACCTTCTTCGAGCGCCGCGGGCGCCTGCGTCATGCCATGCAATGGTACACGCGGA
TCGGACGCCGGGTCTGGCCCTACGAGATCTGGCAGTTCCTGCGAGCCCGGCATTTGAAGACCGGACCGACCGTCGGCGAA
TTCTGGGGCGACGGCCAAGTGGTCGATGAGAACGCGATGGCCACATCGCGGCAACGACGCCAGCTTCCCAATCAAAGCGC
CGCCTGA

Upstream 100 bases:

>100_bases
GACGCCATGGCGTCGTTCCATGGCCGTGAGCGCCGGTTCGGCGGCCTGCCATCGATGAAACCGGACGTCTCCATCAGCGC
CTAGGCGAAGGAAGTCCGTG

Downstream 100 bases:

>100_bases
GTGGGGTGTGTAACGGACAGTGCTCCAGCGGCCCCAGCCCACATCCGTAGTTCGCATAAGGTATATTATGGAATATTCAA
ATGTGCCAATTCGGCCAGAG

Product: magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase

Products: 5'-deoxyadenosine; methionine; flavodoxin; formate acetyltransferase-glycine-2-yl radical

Alternate protein names: Mg-protoporphyrin IX monomethyl ester oxidative cyclase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MRILLVNVPHPAIGSLIPSDHLPPLGLLAIGGPLIDDGHDVRLLDAEFGPTSTAQIVGQARDFRPDAVLFGHSGSTSGHP
VIAEVAQAIAHAIPGTRIVYGGVFPTYHWREILDAEPYVTAIVRGEGEETARRLMTALADGDDLAGVHGIAYRRAGQACA
TPPAVVIGDLDAYRIGWELIDHARYSYWGGLRAVVVQFSRGCPHLCSYCGQRGFWTRWRHRDPVLFAKELARLHREQGVR
VVNFADENPTVSKKVWQTFLEALIAEEVDLILVGSTRADDIVRDANILHLYKQAGWDRFLLGLENTDDATLALIRKGAAT
PTDREAIRLLRRHGILSMATWVVGFVEETDRDHWRGLRQLLSYDPDQIQMLYATPHRWTPYFGQAAERRVITTDRRHWDY
KHQVLANRNMPPWRVLLWFKFTELVLQARPKAMFRTFFERRGRLRHAMQWYTRIGRRVWPYEIWQFLRARHLKTGPTVGE
FWGDGQVVDENAMATSRQRRQLPNQSAA

Sequences:

>Translated_508_residues
MRILLVNVPHPAIGSLIPSDHLPPLGLLAIGGPLIDDGHDVRLLDAEFGPTSTAQIVGQARDFRPDAVLFGHSGSTSGHP
VIAEVAQAIAHAIPGTRIVYGGVFPTYHWREILDAEPYVTAIVRGEGEETARRLMTALADGDDLAGVHGIAYRRAGQACA
TPPAVVIGDLDAYRIGWELIDHARYSYWGGLRAVVVQFSRGCPHLCSYCGQRGFWTRWRHRDPVLFAKELARLHREQGVR
VVNFADENPTVSKKVWQTFLEALIAEEVDLILVGSTRADDIVRDANILHLYKQAGWDRFLLGLENTDDATLALIRKGAAT
PTDREAIRLLRRHGILSMATWVVGFVEETDRDHWRGLRQLLSYDPDQIQMLYATPHRWTPYFGQAAERRVITTDRRHWDY
KHQVLANRNMPPWRVLLWFKFTELVLQARPKAMFRTFFERRGRLRHAMQWYTRIGRRVWPYEIWQFLRARHLKTGPTVGE
FWGDGQVVDENAMATSRQRRQLPNQSAA
>Mature_508_residues
MRILLVNVPHPAIGSLIPSDHLPPLGLLAIGGPLIDDGHDVRLLDAEFGPTSTAQIVGQARDFRPDAVLFGHSGSTSGHP
VIAEVAQAIAHAIPGTRIVYGGVFPTYHWREILDAEPYVTAIVRGEGEETARRLMTALADGDDLAGVHGIAYRRAGQACA
TPPAVVIGDLDAYRIGWELIDHARYSYWGGLRAVVVQFSRGCPHLCSYCGQRGFWTRWRHRDPVLFAKELARLHREQGVR
VVNFADENPTVSKKVWQTFLEALIAEEVDLILVGSTRADDIVRDANILHLYKQAGWDRFLLGLENTDDATLALIRKGAAT
PTDREAIRLLRRHGILSMATWVVGFVEETDRDHWRGLRQLLSYDPDQIQMLYATPHRWTPYFGQAAERRVITTDRRHWDY
KHQVLANRNMPPWRVLLWFKFTELVLQARPKAMFRTFFERRGRLRHAMQWYTRIGRRVWPYEIWQFLRARHLKTGPTVGE
FWGDGQVVDENAMATSRQRRQLPNQSAA

Specific function: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis in anaerobic conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-prot

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011772
- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: 1.97.1.4

Molecular weight: Translated: 57813; Mature: 57813

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLVNVPHPAIGSLIPSDHLPPLGLLAIGGPLIDDGHDVRLLDAEFGPTSTAQIVGQA
CEEEEEECCCHHHHCCCCCCCCCCCCHHEECCCEECCCCCEEEEECCCCCCHHHHHHHHH
RDFRPDAVLFGHSGSTSGHPVIAEVAQAIAHAIPGTRIVYGGVFPTYHWREILDAEPYVT
HCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEE
AIVRGEGEETARRLMTALADGDDLAGVHGIAYRRAGQACATPPAVVIGDLDAYRIGWELI
EEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
DHARYSYWGGLRAVVVQFSRGCPHLCSYCGQRGFWTRWRHRDPVLFAKELARLHREQGVR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCE
VVNFADENPTVSKKVWQTFLEALIAEEVDLILVGSTRADDIVRDANILHLYKQAGWDRFL
EEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCHHHHHHHHCCCCEEE
LGLENTDDATLALIRKGAATPTDREAIRLLRRHGILSMATWVVGFVEETDRDHWRGLRQL
EECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHH
LSYDPDQIQMLYATPHRWTPYFGQAAERRVITTDRRHWDYKHQVLANRNMPPWRVLLWFK
HCCCCCCEEEEEECCCCCCCCCCHHHCCCEEECCCCCCCHHHHHHHCCCCCCCEEHHHHH
FTELVLQARPKAMFRTFFERRGRLRHAMQWYTRIGRRVWPYEIWQFLRARHLKTGPTVGE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHH
FWGDGQVVDENAMATSRQRRQLPNQSAA
HCCCCEEECCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRILLVNVPHPAIGSLIPSDHLPPLGLLAIGGPLIDDGHDVRLLDAEFGPTSTAQIVGQA
CEEEEEECCCHHHHCCCCCCCCCCCCHHEECCCEECCCCCEEEEECCCCCCHHHHHHHHH
RDFRPDAVLFGHSGSTSGHPVIAEVAQAIAHAIPGTRIVYGGVFPTYHWREILDAEPYVT
HCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCEEE
AIVRGEGEETARRLMTALADGDDLAGVHGIAYRRAGQACATPPAVVIGDLDAYRIGWELI
EEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
DHARYSYWGGLRAVVVQFSRGCPHLCSYCGQRGFWTRWRHRDPVLFAKELARLHREQGVR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCE
VVNFADENPTVSKKVWQTFLEALIAEEVDLILVGSTRADDIVRDANILHLYKQAGWDRFL
EEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCHHHHHHHHCCCCEEE
LGLENTDDATLALIRKGAATPTDREAIRLLRRHGILSMATWVVGFVEETDRDHWRGLRQL
EECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHH
LSYDPDQIQMLYATPHRWTPYFGQAAERRVITTDRRHWDYKHQVLANRNMPPWRVLLWFK
HCCCCCCEEEEEECCCCCCCCCCHHHCCCEEECCCCCCCHHHHHHHCCCCCCCEEHHHHH
FTELVLQARPKAMFRTFFERRGRLRHAMQWYTRIGRRVWPYEIWQFLRARHLKTGPTVGE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHH
FWGDGQVVDENAMATSRQRRQLPNQSAA
HCCCCEEECCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; dihydroflavodoxin; formate acetyltransferase-glycine

Specific reaction: S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine = 5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl radical

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]