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The map label for this gene is yngI [H]

Identifier: 86749801

GI number: 86749801

Start: 3066330

End: 3067979

Strand: Reverse

Name: yngI [H]

Synonym: RPB_2684

Alternate gene names: 86749801

Gene position: 3067979-3066330 (Counterclockwise)

Preceding gene: 86749802

Following gene: 86749794

Centisome position: 57.54

GC content: 66.06

Gene sequence:

>1650_bases
ATGAGCGTTCCGCAAGGTCACTACAGCATCGGGCTGGACAAGACGCCGGCGAATTACGTGCCGCTGTCGCCGCTGAGTTT
CCTTGAACGCACCGCCAACGTCTATCCGGACCACACCAGCGTGGTCTACGAAGGCCGCCACTACACCTGGAAGGAGACTC
GCGCGCGCTGCCGCCGCTTCGCCTCCTGGCTGTCGCGCAGCGGCATCGGCCGCGGCGACACCGTGGCGCTGATGCTGCCC
AATGTGCCGGCGATGGTCGAGGCGCATTTCGCGGTGCCGATGGCCGGCGCGGTGCTGAACGCGCTGAACATTCGGCTCGA
CGCGCCGGCGATCGCGTTTCAGCTCGAGCATGGCGGCGCCAAGATCATCCTGGTCGACCCTGAATTCTCCGCGGTGATCG
CCGACGCGCTGAAGCAGATGAAGGGTCCGAAACCGCTGGTGATCGACGTCGACGACAAGATGTTTCCCGGCCATCACCGC
ATCGGCGAACTCGAATACGAATACGCCGTCGCCTCCGGCGATCCCTGCTTCGTGGGCCAGCGGCCGCAGGACGAATGGGA
TGCGATCGCGCTCGGCTACACCTCCGGCACGACGGGCAATCCCAAGGGCGTGGTGACGCATCATCGCGGCGCGTATCTGA
ACGCCGTCAGCAACATCCTCGCCGCCAACCTCGGCAGCCATCCGGTGTATCTGTGGACGCTGCCGATGTTCCACTGCAAC
GGCTGGTGCTTCCCCTGGACCATCGCGGCCGCGGCCGGCGTCAATGTCTGCCTGCGCAAGGTCGATCCGGCAAAAATCTT
CGATCTGATCCCGCGCCACGGCGTCACCCACATGTCCGGCGCGCCGATCGTCTACAACGCGCTGATCAATGCTCCCGAGG
CGCCGAAGGGCGCGGCCGCGCGTCCCGTCGTCGGCCTGATCGCCGGCGCCGCGCCGCCGGTCGCGGTGCTGGCCGGCGCC
GAGCAGGTCGGCATCAAGCTGACCCACGTCTACGGCCTCACCGAAGTCTACGGCCCCGCCTCGGTCTGCGCCGAGCAGCC
CGGCTGGGACGACCTGCCCGTCGCCGAGCGCGCCCAGCTCAAGCGCCGGCAGGGCGTGCCCTACCCGCTGCAGGAAGCCG
TCACCGTGCTCGATCCCGAGACGATGCAGGAAGTACCGCGCGACGGCGAGACCATCGGCGAGGTGATGTTTCGCGGCAAC
ATCGTGATGAAGGGCTATCTGAAGAACGAGAAGGCCACGAAGGAAGCGCTGGCCGGCGGCTGGTTTCACACCGGCGACCT
CGGCGTGCTCGACGCGCACGGCTACGTCATCATCAAGGACCGCTCGAAAGACATCATCATCTCCGGCGGCGAGAACGTCT
CCTCGGTCGAGGTCGAGGACGTGCTGTACAAGCACCCGGCGATCCTGTTCGCCGCCGTGGTCGCCAAGCCCGATCCGAAA
TGGGGCGAAGTCCCCTGCGCCTTCGTCGAACTGAAGGACGGCGCCAGCGCCAGCGAGGCCGAGATCATCGCCTATTGCCG
CGAACACCTGCCCGGCTTCAAGACGCCGAAGACCATCGTGTTCTCGGCGATCCCGAAGACCTCCACCGGCAAGATCCAGA
AATTCATGCTGCGCGATCAGGTGAAATCGGCGAAGGCGATTTCGGAGTAG

Upstream 100 bases:

>100_bases
TGATCATCGTCGCCGCGGTGCCGTGGATTTCGATCGGATTTCTGTGAGGCGTTGCGGGTCTTCGGACGCGCCGCCATCGT
GAACTGAGAGGCAGGTCGTC

Downstream 100 bases:

>100_bases
TTACTTGTCGTCATTCCGGGGGCCGGCGTCCGCAGTCGGCGACAACCTCTCGTTCGTCATTCCGGGGCGCTCGCGAAGCG
AGCGAGCCCGGAATCCATAC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSVPQGHYSIGLDKTPANYVPLSPLSFLERTANVYPDHTSVVYEGRHYTWKETRARCRRFASWLSRSGIGRGDTVALMLP
NVPAMVEAHFAVPMAGAVLNALNIRLDAPAIAFQLEHGGAKIILVDPEFSAVIADALKQMKGPKPLVIDVDDKMFPGHHR
IGELEYEYAVASGDPCFVGQRPQDEWDAIALGYTSGTTGNPKGVVTHHRGAYLNAVSNILAANLGSHPVYLWTLPMFHCN
GWCFPWTIAAAAGVNVCLRKVDPAKIFDLIPRHGVTHMSGAPIVYNALINAPEAPKGAAARPVVGLIAGAAPPVAVLAGA
EQVGIKLTHVYGLTEVYGPASVCAEQPGWDDLPVAERAQLKRRQGVPYPLQEAVTVLDPETMQEVPRDGETIGEVMFRGN
IVMKGYLKNEKATKEALAGGWFHTGDLGVLDAHGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
WGEVPCAFVELKDGASASEAEIIAYCREHLPGFKTPKTIVFSAIPKTSTGKIQKFMLRDQVKSAKAISE

Sequences:

>Translated_549_residues
MSVPQGHYSIGLDKTPANYVPLSPLSFLERTANVYPDHTSVVYEGRHYTWKETRARCRRFASWLSRSGIGRGDTVALMLP
NVPAMVEAHFAVPMAGAVLNALNIRLDAPAIAFQLEHGGAKIILVDPEFSAVIADALKQMKGPKPLVIDVDDKMFPGHHR
IGELEYEYAVASGDPCFVGQRPQDEWDAIALGYTSGTTGNPKGVVTHHRGAYLNAVSNILAANLGSHPVYLWTLPMFHCN
GWCFPWTIAAAAGVNVCLRKVDPAKIFDLIPRHGVTHMSGAPIVYNALINAPEAPKGAAARPVVGLIAGAAPPVAVLAGA
EQVGIKLTHVYGLTEVYGPASVCAEQPGWDDLPVAERAQLKRRQGVPYPLQEAVTVLDPETMQEVPRDGETIGEVMFRGN
IVMKGYLKNEKATKEALAGGWFHTGDLGVLDAHGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
WGEVPCAFVELKDGASASEAEIIAYCREHLPGFKTPKTIVFSAIPKTSTGKIQKFMLRDQVKSAKAISE
>Mature_548_residues
SVPQGHYSIGLDKTPANYVPLSPLSFLERTANVYPDHTSVVYEGRHYTWKETRARCRRFASWLSRSGIGRGDTVALMLPN
VPAMVEAHFAVPMAGAVLNALNIRLDAPAIAFQLEHGGAKIILVDPEFSAVIADALKQMKGPKPLVIDVDDKMFPGHHRI
GELEYEYAVASGDPCFVGQRPQDEWDAIALGYTSGTTGNPKGVVTHHRGAYLNAVSNILAANLGSHPVYLWTLPMFHCNG
WCFPWTIAAAAGVNVCLRKVDPAKIFDLIPRHGVTHMSGAPIVYNALINAPEAPKGAAARPVVGLIAGAAPPVAVLAGAE
QVGIKLTHVYGLTEVYGPASVCAEQPGWDDLPVAERAQLKRRQGVPYPLQEAVTVLDPETMQEVPRDGETIGEVMFRGNI
VMKGYLKNEKATKEALAGGWFHTGDLGVLDAHGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPKW
GEVPCAFVELKDGASASEAEIIAYCREHLPGFKTPKTIVFSAIPKTSTGKIQKFMLRDQVKSAKAISE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=29.8913043478261, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI187761345, Length=583, Percent_Identity=25.557461406518, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI187761343, Length=583, Percent_Identity=25.557461406518, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI157311624, Length=526, Percent_Identity=26.9961977186312, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI157311622, Length=526, Percent_Identity=26.9961977186312, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI122937307, Length=518, Percent_Identity=25.8687258687259, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI42544132, Length=517, Percent_Identity=26.8858800773694, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI58082049, Length=523, Percent_Identity=26.1950286806883, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI38505220, Length=493, Percent_Identity=26.5720081135903, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI115511026, Length=520, Percent_Identity=26.3461538461538, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI13375727, Length=543, Percent_Identity=25.0460405156538, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI27477105, Length=495, Percent_Identity=24.4444444444444, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1788107, Length=559, Percent_Identity=29.1592128801431, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI145693145, Length=530, Percent_Identity=30.5660377358491, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI221142682, Length=525, Percent_Identity=25.9047619047619, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786810, Length=541, Percent_Identity=25.6931608133087, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788595, Length=504, Percent_Identity=25.7936507936508, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790505, Length=557, Percent_Identity=22.6211849192101, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=554, Percent_Identity=28.158844765343, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71994703, Length=554, Percent_Identity=28.158844765343, Blast_Score=189, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71994694, Length=554, Percent_Identity=28.158844765343, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17538037, Length=521, Percent_Identity=28.2149712092131, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=511, Percent_Identity=28.3757338551859, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=520, Percent_Identity=27.1153846153846, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=551, Percent_Identity=25.5898366606171, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=522, Percent_Identity=26.6283524904215, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=467, Percent_Identity=26.9807280513919, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=538, Percent_Identity=24.3494423791822, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=514, Percent_Identity=23.9299610894942, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=527, Percent_Identity=23.1499051233397, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=565, Percent_Identity=24.4247787610619, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982997, Length=565, Percent_Identity=24.4247787610619, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=374, Percent_Identity=28.8770053475936, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=495, Percent_Identity=25.4545454545455, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=565, Percent_Identity=23.716814159292, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323182, Length=135, Percent_Identity=32.5925925925926, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24581924, Length=557, Percent_Identity=28.1867145421903, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI21355181, Length=525, Percent_Identity=26.8571428571429, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859661, Length=523, Percent_Identity=28.2982791586998, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24656500, Length=539, Percent_Identity=24.6753246753247, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI45552801, Length=544, Percent_Identity=23.5294117647059, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550496, Length=544, Percent_Identity=23.5294117647059, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.6452905811623, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648260, Length=520, Percent_Identity=24.0384615384615, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281365686, Length=512, Percent_Identity=22.65625, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921316, Length=509, Percent_Identity=23.5756385068762, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583465, Length=560, Percent_Identity=22.1428571428571, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583463, Length=560, Percent_Identity=22.1428571428571, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356947, Length=520, Percent_Identity=22.6923076923077, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364798, Length=560, Percent_Identity=22.1428571428571, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20130357, Length=368, Percent_Identity=22.8260869565217, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17933690, Length=82, Percent_Identity=42.6829268292683, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59285; Mature: 59154

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPQGHYSIGLDKTPANYVPLSPLSFLERTANVYPDHTSVVYEGRHYTWKETRARCRRF
CCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHH
ASWLSRSGIGRGDTVALMLPNVPAMVEAHFAVPMAGAVLNALNIRLDAPAIAFQLEHGGA
HHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCE
KIILVDPEFSAVIADALKQMKGPKPLVIDVDDKMFPGHHRIGELEYEYAVASGDPCFVGQ
EEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCEEECCC
RPQDEWDAIALGYTSGTTGNPKGVVTHHRGAYLNAVSNILAANLGSHPVYLWTLPMFHCN
CCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECHHHCC
GWCFPWTIAAAAGVNVCLRKVDPAKIFDLIPRHGVTHMSGAPIVYNALINAPEAPKGAAA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHCCCCCCCCCCC
RPVVGLIAGAAPPVAVLAGAEQVGIKLTHVYGLTEVYGPASVCAEQPGWDDLPVAERAQL
CCHHHHHHCCCCCEEEEECCHHHCEEEEEEECHHHHCCCHHHHCCCCCCCCCCHHHHHHH
KRRQGVPYPLQEAVTVLDPETMQEVPRDGETIGEVMFRGNIVMKGYLKNEKATKEALAGG
HHHCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCC
WFHTGDLGVLDAHGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
CEECCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCCCC
WGEVPCAFVELKDGASASEAEIIAYCREHLPGFKTPKTIVFSAIPKTSTGKIQKFMLRDQ
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
VKSAKAISE
HHHHHHCCC
>Mature Secondary Structure 
SVPQGHYSIGLDKTPANYVPLSPLSFLERTANVYPDHTSVVYEGRHYTWKETRARCRRF
CCCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHH
ASWLSRSGIGRGDTVALMLPNVPAMVEAHFAVPMAGAVLNALNIRLDAPAIAFQLEHGGA
HHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCE
KIILVDPEFSAVIADALKQMKGPKPLVIDVDDKMFPGHHRIGELEYEYAVASGDPCFVGQ
EEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCEEECCC
RPQDEWDAIALGYTSGTTGNPKGVVTHHRGAYLNAVSNILAANLGSHPVYLWTLPMFHCN
CCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECHHHCC
GWCFPWTIAAAAGVNVCLRKVDPAKIFDLIPRHGVTHMSGAPIVYNALINAPEAPKGAAA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHCCCCCCCCCCC
RPVVGLIAGAAPPVAVLAGAEQVGIKLTHVYGLTEVYGPASVCAEQPGWDDLPVAERAQL
CCHHHHHHCCCCCEEEEECCHHHCEEEEEEECHHHHCCCHHHHCCCCCCCCCCHHHHHHH
KRRQGVPYPLQEAVTVLDPETMQEVPRDGETIGEVMFRGNIVMKGYLKNEKATKEALAGG
HHHCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCC
WFHTGDLGVLDAHGYVIIKDRSKDIIISGGENVSSVEVEDVLYKHPAILFAAVVAKPDPK
CEECCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHCCCCCC
WGEVPCAFVELKDGASASEAEIIAYCREHLPGFKTPKTIVFSAIPKTSTGKIQKFMLRDQ
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
VKSAKAISE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]