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The map label for this gene is yfhM [H]

Identifier: 86749794

GI number: 86749794

Start: 3058720

End: 3059667

Strand: Reverse

Name: yfhM [H]

Synonym: RPB_2677

Alternate gene names: 86749794

Gene position: 3059667-3058720 (Counterclockwise)

Preceding gene: 86749801

Following gene: 86749792

Centisome position: 57.39

GC content: 65.3

Gene sequence:

>948_bases
ATGGAAACGACCAGGACCGTTGCGGTCAACGGGATCGAACTGTTCTTGCGCGAACGCGGCGAAGGCCCGCTGGTGCTGCT
GTGCCACGGCTGGCCGGAACTGTCGTATTCATGGCGGCACCAGATCCCGGCGCTCGCCGACGCCGGCTATCGCGTGGTCG
CGCCCGACATGCGCGGTTTCGGGCGCTCCTCCGCGCCGCATCCGATCGAGGCTTACAGCATCTTCGATCTGGTCGGCGAC
ATGGTGGCGCTGGTCGCCGAGTTGAAGGAGACCCGCGCGGTCATCATCGGCCACGACTGGGGCGCGCCGGTCGCCTGGCA
TGCGGCGTTGTTCCGGCCGGAGCTGTTCACTGCCGTCGCGGGCCTGAGCGTGCCGCCCCCCTGGCGCGGCAAGGGCCGGC
CGCTGGACCTTCTGAGCGCTGCAGGCGTCACCAACTTCTACTGGCAATATTTCCAGACACCCGGCGTCGCCGAGGCGGAA
TTCGAACGTGACGTGACCGCGACGATCCGGGGGATGCTGTGCGGCGGGTTTTCAGATCCGACGCGCTCGCTGTTCGTGCC
GGAAGGGCGCGGATTCATCGGCCGGTCGGCGTCATCGCTCCCGCTGCCGAGCTGGCTGAGCGAAACCGAACTCGCGTTCT
TCATCGAACAGTACAAGGAGAGCGGCTTTCGCGGCGGGTTGAACTGGTACCGCAATATCGACCGCAATTGGGACCTGACC
GCCCCCTGGGATGGCGCGCAAATTCGTCAGCCGGCTTTGTTCATCGCCGGCAGCAAAGACCCGGTGATCTCCGACAAGAT
GAGCGGCAAGCATGTCGCCGCAATGGAGCGGGTGCTGCCGAATCTGAAGCGGAAGCTGATCATCGACGGCGCCGGCCACT
GGATTCAGCAGGAGAAGCCCGCGGAGGTGAATGCCGCGCTGATCGATTTCCTGAAACAGGTGACGTGA

Upstream 100 bases:

>100_bases
AGGAGTGGACTGTTCTCCATTGTGGCGACGCCGGATCGATCCTATCGTGCGGGCCGCTCGGCTTCCACATAAACTCCAAT
CAATTCAGGGCGGAAACGCG

Downstream 100 bases:

>100_bases
GCCGCGGCTCAGTACAGCCCGCGCGCCGACAGCAGGCTGCCGATCTCGTCACTGGCGCGCGACGGCGCCTCTGCGCGAGC
GGCGGTCTCGGCGTAGCTGC

Product: Alpha/beta hydrolase

Products: glycol

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
METTRTVAVNGIELFLRERGEGPLVLLCHGWPELSYSWRHQIPALADAGYRVVAPDMRGFGRSSAPHPIEAYSIFDLVGD
MVALVAELKETRAVIIGHDWGAPVAWHAALFRPELFTAVAGLSVPPPWRGKGRPLDLLSAAGVTNFYWQYFQTPGVAEAE
FERDVTATIRGMLCGGFSDPTRSLFVPEGRGFIGRSASSLPLPSWLSETELAFFIEQYKESGFRGGLNWYRNIDRNWDLT
APWDGAQIRQPALFIAGSKDPVISDKMSGKHVAAMERVLPNLKRKLIIDGAGHWIQQEKPAEVNAALIDFLKQVT

Sequences:

>Translated_315_residues
METTRTVAVNGIELFLRERGEGPLVLLCHGWPELSYSWRHQIPALADAGYRVVAPDMRGFGRSSAPHPIEAYSIFDLVGD
MVALVAELKETRAVIIGHDWGAPVAWHAALFRPELFTAVAGLSVPPPWRGKGRPLDLLSAAGVTNFYWQYFQTPGVAEAE
FERDVTATIRGMLCGGFSDPTRSLFVPEGRGFIGRSASSLPLPSWLSETELAFFIEQYKESGFRGGLNWYRNIDRNWDLT
APWDGAQIRQPALFIAGSKDPVISDKMSGKHVAAMERVLPNLKRKLIIDGAGHWIQQEKPAEVNAALIDFLKQVT
>Mature_315_residues
METTRTVAVNGIELFLRERGEGPLVLLCHGWPELSYSWRHQIPALADAGYRVVAPDMRGFGRSSAPHPIEAYSIFDLVGD
MVALVAELKETRAVIIGHDWGAPVAWHAALFRPELFTAVAGLSVPPPWRGKGRPLDLLSAAGVTNFYWQYFQTPGVAEAE
FERDVTATIRGMLCGGFSDPTRSLFVPEGRGFIGRSASSLPLPSWLSETELAFFIEQYKESGFRGGLNWYRNIDRNWDLT
APWDGAQIRQPALFIAGSKDPVISDKMSGKHVAAMERVLPNLKRKLIIDGAGHWIQQEKPAEVNAALIDFLKQVT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=301, Percent_Identity=44.8504983388704, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI218777837, Length=303, Percent_Identity=30.03300330033, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI218777840, Length=297, Percent_Identity=27.9461279461279, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI13376164, Length=297, Percent_Identity=27.9461279461279, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17554012, Length=318, Percent_Identity=27.3584905660377, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17562298, Length=311, Percent_Identity=25.4019292604502, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.3.2.3

Molecular weight: Translated: 34914; Mature: 34914

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTRTVAVNGIELFLRERGEGPLVLLCHGWPELSYSWRHQIPALADAGYRVVAPDMRGF
CCCCEEEEECHHHEEEHHCCCCCEEEEECCCCCCCCHHHHCCCHHHCCCCEEECCCHHCC
GRSSAPHPIEAYSIFDLVGDMVALVAELKETRAVIIGHDWGAPVAWHAALFRPELFTAVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCHHHHHHHH
GLSVPPPWRGKGRPLDLLSAAGVTNFYWQYFQTPGVAEAEFERDVTATIRGMLCGGFSDP
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TRSLFVPEGRGFIGRSASSLPLPSWLSETELAFFIEQYKESGFRGGLNWYRNIDRNWDLT
CCEEECCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
APWDGAQIRQPALFIAGSKDPVISDKMSGKHVAAMERVLPNLKRKLIIDGAGHWIQQEKP
CCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
AEVNAALIDFLKQVT
CHHHHHHHHHHHHCC
>Mature Secondary Structure
METTRTVAVNGIELFLRERGEGPLVLLCHGWPELSYSWRHQIPALADAGYRVVAPDMRGF
CCCCEEEEECHHHEEEHHCCCCCEEEEECCCCCCCCHHHHCCCHHHCCCCEEECCCHHCC
GRSSAPHPIEAYSIFDLVGDMVALVAELKETRAVIIGHDWGAPVAWHAALFRPELFTAVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCHHHHHHHH
GLSVPPPWRGKGRPLDLLSAAGVTNFYWQYFQTPGVAEAEFERDVTATIRGMLCGGFSDP
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TRSLFVPEGRGFIGRSASSLPLPSWLSETELAFFIEQYKESGFRGGLNWYRNIDRNWDLT
CCEEECCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
APWDGAQIRQPALFIAGSKDPVISDKMSGKHVAAMERVLPNLKRKLIIDGAGHWIQQEKP
CCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
AEVNAALIDFLKQVT
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; epoxide

Specific reaction: H2O + an epoxide = a glycol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]