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The map label for this gene is hgiDIIM [H]

Identifier: 86749313

GI number: 86749313

Start: 2486279

End: 2487355

Strand: Direct

Name: hgiDIIM [H]

Synonym: RPB_2193

Alternate gene names: 86749313

Gene position: 2486279-2487355 (Clockwise)

Preceding gene: 86749312

Following gene: 86749318

Centisome position: 46.63

GC content: 53.95

Gene sequence:

>1077_bases
ATGGGGAATCACGCGATGCAACGAGAAGTGCCGTCGAGGATTGTGGCGGTCGACCTTTTTTGCGGGGCCGCTGGCCTTTC
ATTGGGCTTGAAGATCAGCGGGATAAAGGTCGCAGCCGGAATTGATCTCGATCCGGCATGTCGTTTCCCTTTCGAGACCA
ATATTGGAGCCATGTTTGTTGAAGCGGACGTTTCCAGCCTGTCGGGGAAGGCCGTAGATAGCCTATTTGGCGATGCCTCG
ATTCGAGTTTTGGCCGGATGTGCACCGTGCCAGCCGTTCTCAGGATACACGACAAAGCGACGTTCAATTGATCGTCGGTG
GGAACTGCTACTAGAATTTCTGCGCATAGTTCGTGAGTCAGATCCGGACATTGTCACCCTAGAGAATGTGCCTCGCCTCG
CGAATCTCCCGCTTTGGCAAGACTTCGTTACAGACCTCAAGTCGCAAGGTTACTTCCTGAATTGGGATGTACTGGATGTC
TCCAAATTCGGAGTGCCCCAGAATCGTCGGAGACTGGTACTCTTGGCGTCCAAATTTGGAGATATTCGACTTCCCGAGCC
GATTGGAACACCGCCGACAACGGTGAAGAAGGCAATCGGCAAGCTTTCCCCTGTGAAAGCGGGAGAAGCCAATCCTGTCG
ATCCGCTTCATGCGTCTCGCGCTTTGACGCCGCGCAATCTGGCACGAATTCGCCAATCAAGGCCGGCGGGCACCTGGCGG
GAATGGCCAAAGCGTATGCGTGTCGCATGTCACAAGACTGGGTCTGGAAAGACCTATCCGTCCGTGTATGGACGTATGTC
ATGGGACAAACCCTCACCGACGATTACAACTCAGTTCTACGGCTTTGGGAATGGTCGCTTCGGCCACCCGGAGCAAGATC
GTGCGATTACTCTCCGAGAGGGAGCCATTCTGCAATCGTTTCCTCGAGATTTTCAGTTTGCTCCGACTGGGAGTCGTGTG
AATTTTCGAGGTATAGGCAGATTGATCGGAAATGCTGTGCCGCCGGCCCTTGGCTTGGCAATCGGTAACGCCATTCTAAA
TCACGTTACTGCGCTAAAACGCACTGATAGAGGCTGA

Upstream 100 bases:

>100_bases
GCCAAATCAGATGGGCCGTCCAAGATCAAATCCCATCACCGCGTGTGCCTTAGGGCAAAAAAAGATCAAAATCCCCTGCG
TGCTAGGGTAATTTCCGATA

Downstream 100 bases:

>100_bases
ATGTCGAGCGCGATTGGTGGCAATTCCAGATAGCGCTCGTGTTCCAAATAGTATGCGACATTGCTCAATATTCCGTCCAT
ATATCGCATTGATCGTCGTG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MGNHAMQREVPSRIVAVDLFCGAAGLSLGLKISGIKVAAGIDLDPACRFPFETNIGAMFVEADVSSLSGKAVDSLFGDAS
IRVLAGCAPCQPFSGYTTKRRSIDRRWELLLEFLRIVRESDPDIVTLENVPRLANLPLWQDFVTDLKSQGYFLNWDVLDV
SKFGVPQNRRRLVLLASKFGDIRLPEPIGTPPTTVKKAIGKLSPVKAGEANPVDPLHASRALTPRNLARIRQSRPAGTWR
EWPKRMRVACHKTGSGKTYPSVYGRMSWDKPSPTITTQFYGFGNGRFGHPEQDRAITLREGAILQSFPRDFQFAPTGSRV
NFRGIGRLIGNAVPPALGLAIGNAILNHVTALKRTDRG

Sequences:

>Translated_358_residues
MGNHAMQREVPSRIVAVDLFCGAAGLSLGLKISGIKVAAGIDLDPACRFPFETNIGAMFVEADVSSLSGKAVDSLFGDAS
IRVLAGCAPCQPFSGYTTKRRSIDRRWELLLEFLRIVRESDPDIVTLENVPRLANLPLWQDFVTDLKSQGYFLNWDVLDV
SKFGVPQNRRRLVLLASKFGDIRLPEPIGTPPTTVKKAIGKLSPVKAGEANPVDPLHASRALTPRNLARIRQSRPAGTWR
EWPKRMRVACHKTGSGKTYPSVYGRMSWDKPSPTITTQFYGFGNGRFGHPEQDRAITLREGAILQSFPRDFQFAPTGSRV
NFRGIGRLIGNAVPPALGLAIGNAILNHVTALKRTDRG
>Mature_357_residues
GNHAMQREVPSRIVAVDLFCGAAGLSLGLKISGIKVAAGIDLDPACRFPFETNIGAMFVEADVSSLSGKAVDSLFGDASI
RVLAGCAPCQPFSGYTTKRRSIDRRWELLLEFLRIVRESDPDIVTLENVPRLANLPLWQDFVTDLKSQGYFLNWDVLDVS
KFGVPQNRRRLVLLASKFGDIRLPEPIGTPPTTVKKAIGKLSPVKAGEANPVDPLHASRALTPRNLARIRQSRPAGTWRE
WPKRMRVACHKTGSGKTYPSVYGRMSWDKPSPTITTQFYGFGNGRFGHPEQDRAITLREGAILQSFPRDFQFAPTGSRVN
FRGIGRLIGNAVPPALGLAIGNAILNHVTALKRTDRG

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39286; Mature: 39155

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHAMQREVPSRIVAVDLFCGAAGLSLGLKISGIKVAAGIDLDPACRFPFETNIGAMFV
CCCHHHHHHHHHHEEEEEEHHCCCCCCCCEEEECEEEEECCCCCCCCCCCCCCCCCEEEE
EADVSSLSGKAVDSLFGDASIRVLAGCAPCQPFSGYTTKRRSIDRRWELLLEFLRIVRES
ECCHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DPDIVTLENVPRLANLPLWQDFVTDLKSQGYFLNWDVLDVSKFGVPQNRRRLVLLASKFG
CCCEEEECCCCCHHCCCHHHHHHHHHHHCCEEEEEEEEEHHHCCCCCCCCEEEEEEECCC
DIRLPEPIGTPPTTVKKAIGKLSPVKAGEANPVDPLHASRALTPRNLARIRQSRPAGTWR
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHH
EWPKRMRVACHKTGSGKTYPSVYGRMSWDKPSPTITTQFYGFGNGRFGHPEQDRAITLRE
HHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEC
GAILQSFPRDFQFAPTGSRVNFRGIGRLIGNAVPPALGLAIGNAILNHVTALKRTDRG
CCHHHHCCCCCEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GNHAMQREVPSRIVAVDLFCGAAGLSLGLKISGIKVAAGIDLDPACRFPFETNIGAMFV
CCHHHHHHHHHHEEEEEEHHCCCCCCCCEEEECEEEEECCCCCCCCCCCCCCCCCEEEE
EADVSSLSGKAVDSLFGDASIRVLAGCAPCQPFSGYTTKRRSIDRRWELLLEFLRIVRES
ECCHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DPDIVTLENVPRLANLPLWQDFVTDLKSQGYFLNWDVLDVSKFGVPQNRRRLVLLASKFG
CCCEEEECCCCCHHCCCHHHHHHHHHHHCCEEEEEEEEEHHHCCCCCCCCEEEEEEECCC
DIRLPEPIGTPPTTVKKAIGKLSPVKAGEANPVDPLHASRALTPRNLARIRQSRPAGTWR
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHH
EWPKRMRVACHKTGSGKTYPSVYGRMSWDKPSPTITTQFYGFGNGRFGHPEQDRAITLRE
HHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEC
GAILQSFPRDFQFAPTGSRVNFRGIGRLIGNAVPPALGLAIGNAILNHVTALKRTDRG
CCHHHHCCCCCEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]