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The map label for this gene is mmsB [H]

Identifier: 86749243

GI number: 86749243

Start: 2413052

End: 2413939

Strand: Direct

Name: mmsB [H]

Synonym: RPB_2122

Alternate gene names: 86749243

Gene position: 2413052-2413939 (Clockwise)

Preceding gene: 86749242

Following gene: 86749244

Centisome position: 45.26

GC content: 68.92

Gene sequence:

>888_bases
ATGACGACAATCGCATTCATCGGTCTCGGCAATATGGGCGGGCCGATGGCCGCCAATCTGGTCAAGGCCGGGCGCACCGT
CACCGGCTTCGATCTGTCGGCCGACTCCTGCGACGCAGCCAAGGCCGACGGCGTCGCGATCGCGAATTCCGCGGTCGACG
CGGTGCAGGGCGCCAGCGTGATCGTCACCATGCTGCCCGCCGGCAAGCACGTGCTCGCGGTCTGGAACGAAATCCTGCCG
CATGCGGCCCCGGGCTCGCTGATCATCGACTGCTCGACCATCGACGTCGAAAGCGCCGTGCAGGCGCATACGCTGGCGGC
GAAGGGCGGCATCGCCTCGATCGACGCGCCGGTCTCCGGCGGCACCGGCGGCGCCAAGGCGGCGACGCTGACCTTCATGG
CCGGCGGCAGCGAGGCGGCATTCGCCGCGGCCAAGCCGGTGCTGGAACAGATGGGCAAGAAGATCGTGCATTGCGGCGGC
GCCGGCGCCGGGCAGGCGGCGAAGATCTGCAACAACATGATCCTCGGCATTTCGATGATCGCGGTCGGCGAGGCGTTCAC
GCTGGCCGAAAAGCTCGGCCTGTCGCATCAGGCGCTGTTCGACGTCGCCTCGACCTCCTCGGGGCAGTGCTGGTCGCTGA
CGACCTATTGCCCGGTGCCCGGCCCGGTGCCGACCTCGCCGGCCAACAACGGCTACAAGCCCGGCTTCGCGGCGGCCTTG
ATGCTGAAGGATCTGAAGCTGGCGCAGGAGGCCGCGCAGACCTCCGGCGCCGCGACGCCGCTCGGCGCCCACGCCGCCGA
GCTCTACGCCGCGTTCGAACAGGCCGGCAACGGCGGCACCGATTTTTCCGGGATCATCAACTACCTGCGCGAGCAGGGGC
GGAAGTAA

Upstream 100 bases:

>100_bases
GCCGGACATCGTGGCGCGCTACTTTGACGATCGCGGCGACGACGAGCTGAAATTGCCGGACTGAAGAGCCGGGCCCGCCG
ACAACAAGAAGGGGAGGACT

Downstream 100 bases:

>100_bases
TTGGTCGCTGCCGCTACCACTGAATCGTCATCCTGAGGTGCGAGCCCGTTTGGGCGAGCCTCGAAGGATGCGGAGAAGAG
TGCAGGCGGCCCATCCTTCG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTTIAFIGLGNMGGPMAANLVKAGRTVTGFDLSADSCDAAKADGVAIANSAVDAVQGASVIVTMLPAGKHVLAVWNEILP
HAAPGSLIIDCSTIDVESAVQAHTLAAKGGIASIDAPVSGGTGGAKAATLTFMAGGSEAAFAAAKPVLEQMGKKIVHCGG
AGAGQAAKICNNMILGISMIAVGEAFTLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAAL
MLKDLKLAQEAAQTSGAATPLGAHAAELYAAFEQAGNGGTDFSGIINYLREQGRK

Sequences:

>Translated_295_residues
MTTIAFIGLGNMGGPMAANLVKAGRTVTGFDLSADSCDAAKADGVAIANSAVDAVQGASVIVTMLPAGKHVLAVWNEILP
HAAPGSLIIDCSTIDVESAVQAHTLAAKGGIASIDAPVSGGTGGAKAATLTFMAGGSEAAFAAAKPVLEQMGKKIVHCGG
AGAGQAAKICNNMILGISMIAVGEAFTLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAAL
MLKDLKLAQEAAQTSGAATPLGAHAAELYAAFEQAGNGGTDFSGIINYLREQGRK
>Mature_294_residues
TTIAFIGLGNMGGPMAANLVKAGRTVTGFDLSADSCDAAKADGVAIANSAVDAVQGASVIVTMLPAGKHVLAVWNEILPH
AAPGSLIIDCSTIDVESAVQAHTLAAKGGIASIDAPVSGGTGGAKAATLTFMAGGSEAAFAAAKPVLEQMGKKIVHCGGA
GAGQAAKICNNMILGISMIAVGEAFTLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAALM
LKDLKLAQEAAQTSGAATPLGAHAAELYAAFEQAGNGGTDFSGIINYLREQGRK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=43.5374149659864, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=26.3888888888889, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI145693186, Length=295, Percent_Identity=33.5593220338983, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=32.8859060402685, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786719, Length=296, Percent_Identity=34.4594594594595, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=29.2096219931272, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=297, Percent_Identity=43.0976430976431, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=44.0273037542662, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=44.0273037542662, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29372; Mature: 29241

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAFIGLGNMGGPMAANLVKAGRTVTGFDLSADSCDAAKADGVAIANSAVDAVQGASV
CCEEEEEEECCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHCCCEE
IVTMLPAGKHVLAVWNEILPHAAPGSLIIDCSTIDVESAVQAHTLAAKGGIASIDAPVSG
EEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCCC
GTGGAKAATLTFMAGGSEAAFAAAKPVLEQMGKKIVHCGGAGAGQAAKICNNMILGISMI
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
AVGEAFTLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAAL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
MLKDLKLAQEAAQTSGAATPLGAHAAELYAAFEQAGNGGTDFSGIINYLREQGRK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIAFIGLGNMGGPMAANLVKAGRTVTGFDLSADSCDAAKADGVAIANSAVDAVQGASV
CEEEEEEECCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHCCCEE
IVTMLPAGKHVLAVWNEILPHAAPGSLIIDCSTIDVESAVQAHTLAAKGGIASIDAPVSG
EEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCCC
GTGGAKAATLTFMAGGSEAAFAAAKPVLEQMGKKIVHCGGAGAGQAAKICNNMILGISMI
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHH
AVGEAFTLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAAL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
MLKDLKLAQEAAQTSGAATPLGAHAAELYAAFEQAGNGGTDFSGIINYLREQGRK
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]