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The map label for this gene is hcl [H]

Identifier: 86749190

GI number: 86749190

Start: 2351180

End: 2352550

Strand: Direct

Name: hcl [H]

Synonym: RPB_2069

Alternate gene names: 86749190

Gene position: 2351180-2352550 (Clockwise)

Preceding gene: 86749189

Following gene: 86749191

Centisome position: 44.1

GC content: 72.87

Gene sequence:

>1371_bases
ATGAGCGCGCCGCGCCGCATCGATGATCTGATCGCGTTGATCCGACGCGGACGGGTGCTGCTGCCGGACGGCCGCGACGT
GACGATCGCGGAGGCTTGGCACGACGCCGAGACCGATCCGGCGGCGCGATGGATCGCGCCGGGCGACCGCGTGGTGTTGT
CCGAGCCTGCCGGCGCCGATCTGGTACGCGCCACGCTCGCGGTGTGGGCGCAAGGCGGCGTTGCGGTGTTCGGCGTCGAT
CCGCATCGCTGCGGCGACTGGCCGGCCGATGTTCGCGTGCGGAGCTGGCCGGACGGCGCGGTGCTGCACGATGCGTCGCC
GCGCACCGACGCTGTGGCTGTGATCCATACGTCGTCGGGAACGACGGGGCGGCCGAAGCTGGCGCGCAGGTCGTTCGAGA
GCCTCGCGGCCGAGGCGGAGCGCTATGCCGCGTGCTACGCGCCGGCCGCCGGCGCGCGCGCTTATCTGGCCGCGCCGATC
ACGCACTCGTTTGCATTCGGCGCGATGCTCGGTCTGCTCGCGGCCGGCTGCGTGGTGCAAGTCGCGCCGGTGTTCCGGCC
ACGCGCGCTGGCGGACGCATTGCGCGGCGGCGCCGACATCGTCGTGCTGACGCCGCCGATGGCGCGGCTTGCGATCGAGG
CGGCGCAGGAGCGCGATCAGCCGCTGCGGTGCGCGCCGCAGCTGGTGATCGCCGGCGCCGGCGCCGTGCCGGGCCGGCTC
GACGCGGAGTTTCGCCGCGCATTCGGCTGCGGCCTCGCGCGCAACTACGGCGCGTCGGAAACCGGCGCGAGTTTCGGCGC
GGCGCAGTCGCTGCCCGAGGGCTGCCTCGGCCGGCCGTTCCGCGGCGTCCGTGTCGTGTCGCCCCGGCCGGGCGACCACA
TCGGCGAACTGATCGTCGATCTCGACCACCCGATCCTCAGCCTCGAAGGCAAGACCGCGACGCCACCAATGCAGCGCGGC
CTGTGGCGCAGCGGCGATCTCGCCGAGGTCGACGACGATCAACGGGTGTGGCTGCGCGGACGGATCGACGATCGCGTCAA
GATCAACGGCCATCTGATCGATTGCGCCGCGCTGGCGGCGCAGGCGCGGGCGGCGCCGGGCGTCAGCGATGCGGTGGCGC
TGGCGCTGCCACGGCCGCAGCGCGGCGAGATCCATGATCTGGTGCTGATCTGCGAGTGCCGCGATGAGGCTGTGTCGGCG
GCCGATATCAGCCTGGCTGCGATGCCGCAGACGCCGATGATCGTGCTGACCCGGACCGAATTTCCGCGCACGGCGGCGGG
CAAACCGGATCGCGACCAATTGCGCAGCTTCGCCGAAGCGCGGCTGCGCCGCGGGGCGGCGCGAGGCGAGATGCAAGGCG
AGCCGCGATGA

Upstream 100 bases:

>100_bases
TGCTCGGCGACGACGAATTGTTCGCCGACATCGCCCGCGCGATCGGCGAATTGCGACAGCCGCAACGCGCCGCGACGCCC
CTGTCGCTGTCGGAGACGGC

Downstream 100 bases:

>100_bases
ACGCAGCCGGGCGCAGCATCGAAATCGATCGCTGCGCGCGCGACGCGGTGGCGCTGCTGCCGCATGTCGTGCCGCATCGT
GACGGCCTGCAGACGCTCGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: 3-hydroxybenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSAPRRIDDLIALIRRGRVLLPDGRDVTIAEAWHDAETDPAARWIAPGDRVVLSEPAGADLVRATLAVWAQGGVAVFGVD
PHRCGDWPADVRVRSWPDGAVLHDASPRTDAVAVIHTSSGTTGRPKLARRSFESLAAEAERYAACYAPAAGARAYLAAPI
THSFAFGAMLGLLAAGCVVQVAPVFRPRALADALRGGADIVVLTPPMARLAIEAAQERDQPLRCAPQLVIAGAGAVPGRL
DAEFRRAFGCGLARNYGASETGASFGAAQSLPEGCLGRPFRGVRVVSPRPGDHIGELIVDLDHPILSLEGKTATPPMQRG
LWRSGDLAEVDDDQRVWLRGRIDDRVKINGHLIDCAALAAQARAAPGVSDAVALALPRPQRGEIHDLVLICECRDEAVSA
ADISLAAMPQTPMIVLTRTEFPRTAAGKPDRDQLRSFAEARLRRGAARGEMQGEPR

Sequences:

>Translated_456_residues
MSAPRRIDDLIALIRRGRVLLPDGRDVTIAEAWHDAETDPAARWIAPGDRVVLSEPAGADLVRATLAVWAQGGVAVFGVD
PHRCGDWPADVRVRSWPDGAVLHDASPRTDAVAVIHTSSGTTGRPKLARRSFESLAAEAERYAACYAPAAGARAYLAAPI
THSFAFGAMLGLLAAGCVVQVAPVFRPRALADALRGGADIVVLTPPMARLAIEAAQERDQPLRCAPQLVIAGAGAVPGRL
DAEFRRAFGCGLARNYGASETGASFGAAQSLPEGCLGRPFRGVRVVSPRPGDHIGELIVDLDHPILSLEGKTATPPMQRG
LWRSGDLAEVDDDQRVWLRGRIDDRVKINGHLIDCAALAAQARAAPGVSDAVALALPRPQRGEIHDLVLICECRDEAVSA
ADISLAAMPQTPMIVLTRTEFPRTAAGKPDRDQLRSFAEARLRRGAARGEMQGEPR
>Mature_455_residues
SAPRRIDDLIALIRRGRVLLPDGRDVTIAEAWHDAETDPAARWIAPGDRVVLSEPAGADLVRATLAVWAQGGVAVFGVDP
HRCGDWPADVRVRSWPDGAVLHDASPRTDAVAVIHTSSGTTGRPKLARRSFESLAAEAERYAACYAPAAGARAYLAAPIT
HSFAFGAMLGLLAAGCVVQVAPVFRPRALADALRGGADIVVLTPPMARLAIEAAQERDQPLRCAPQLVIAGAGAVPGRLD
AEFRRAFGCGLARNYGASETGASFGAAQSLPEGCLGRPFRGVRVVSPRPGDHIGELIVDLDHPILSLEGKTATPPMQRGL
WRSGDLAEVDDDQRVWLRGRIDDRVKINGHLIDCAALAAQARAAPGVSDAVALALPRPQRGEIHDLVLICECRDEAVSAA
DISLAAMPQTPMIVLTRTEFPRTAAGKPDRDQLRSFAEARLRRGAARGEMQGEPR

Specific function: Catalyzes the ligation of 3-hydroxybenzoate or 4- hydroxybenzoate and CoA at the expense of ATP. The enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4- chlorobenzoate.

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.27 [H]

Molecular weight: Translated: 48473; Mature: 48342

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRRIDDLIALIRRGRVLLPDGRDVTIAEAWHDAETDPAARWIAPGDRVVLSEPAGAD
CCCCHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCCCCCEEECCCCEEEEECCCCHH
LVRATLAVWAQGGVAVFGVDPHRCGDWPADVRVRSWPDGAVLHDASPRTDAVAVIHTSSG
HHHHHHHHHCCCCEEEEECCHHHCCCCCCCCEEEECCCCCEEECCCCCCCEEEEEEECCC
TTGRPKLARRSFESLAAEAERYAACYAPAAGARAYLAAPITHSFAFGAMLGLLAAGCVVQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
VAPVFRPRALADALRGGADIVVLTPPMARLAIEAAQERDQPLRCAPQLVIAGAGAVPGRL
HHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
DAEFRRAFGCGLARNYGASETGASFGAAQSLPEGCLGRPFRGVRVVSPRPGDHIGELIVD
CHHHHHHHCCCHHCCCCCCCCCCCCCHHHCCCHHHCCCCCCCEEEECCCCCHHHHHHHHC
LDHPILSLEGKTATPPMQRGLWRSGDLAEVDDDQRVWLRGRIDDRVKINGHLIDCAALAA
CCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEHHHHHHH
QARAAPGVSDAVALALPRPQRGEIHDLVLICECRDEAVSAADISLAAMPQTPMIVLTRTE
HHHCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHCEEEEECCCCCEEEEEECC
FPRTAAGKPDRDQLRSFAEARLRRGAARGEMQGEPR
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SAPRRIDDLIALIRRGRVLLPDGRDVTIAEAWHDAETDPAARWIAPGDRVVLSEPAGAD
CCCHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCCCCCEEECCCCEEEEECCCCHH
LVRATLAVWAQGGVAVFGVDPHRCGDWPADVRVRSWPDGAVLHDASPRTDAVAVIHTSSG
HHHHHHHHHCCCCEEEEECCHHHCCCCCCCCEEEECCCCCEEECCCCCCCEEEEEEECCC
TTGRPKLARRSFESLAAEAERYAACYAPAAGARAYLAAPITHSFAFGAMLGLLAAGCVVQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
VAPVFRPRALADALRGGADIVVLTPPMARLAIEAAQERDQPLRCAPQLVIAGAGAVPGRL
HHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
DAEFRRAFGCGLARNYGASETGASFGAAQSLPEGCLGRPFRGVRVVSPRPGDHIGELIVD
CHHHHHHHCCCHHCCCCCCCCCCCCCHHHCCCHHHCCCCCCCEEEECCCCCHHHHHHHHC
LDHPILSLEGKTATPPMQRGLWRSGDLAEVDDDQRVWLRGRIDDRVKINGHLIDCAALAA
CCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEHHHHHHH
QARAAPGVSDAVALALPRPQRGEIHDLVLICECRDEAVSAADISLAAMPQTPMIVLTRTE
HHHCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCHHCEEEEECCCCCEEEEEECC
FPRTAAGKPDRDQLRSFAEARLRRGAARGEMQGEPR
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11208796 [H]