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The map label for this gene is asnB [H]

Identifier: 86749185

GI number: 86749185

Start: 2345626

End: 2347374

Strand: Direct

Name: asnB [H]

Synonym: RPB_2064

Alternate gene names: 86749185

Gene position: 2345626-2347374 (Clockwise)

Preceding gene: 86749184

Following gene: 86749186

Centisome position: 43.99

GC content: 71.18

Gene sequence:

>1749_bases
ATGTGCGCCATAGCCGGACTCGTCTCGCTCGATGCGCCGCCGCACGTGCGGCCGATCCTCGACGCCATCCGCCATCGCGG
CCCGGATGGGCTCGGCACGCATCGCGACGGCGCCTGCGAGATCGGCGCGGCGCGGCTCGCGATCGTCGATCCCGAACGCG
GCGCGCAGCCGATGCGCGATCCGCTGTCGCAGACCGCGGTCGCGTTCAACGGCGAGATCTACAACGCCGACGAATTGCGC
CGCATGCTGATCGCGCGCGGGCACCGCTTCGCGACGCGCTGCGACACGGAAGTGGTGCTGCGCGGCTTCCTCGAATGGGG
GCTGGATCTGCCAACGCATCTGTCCGGCATGTACGCGATCGCGGTGCTGGAACGCGGGCGGCTGACGCTGATCCGCGATC
CGCTCGGCATCAAGCCGCTGCGCTATGCGCAGCGCGGCGCGCAATTCGCGTTCGCCTCGGAGGCCAAGGCGCTGCTGCGC
TGGTTCGGCCCGCCGCGTCTCGACGAGACGGCGTTGGCCGATTTCATCGCGATCGGCTATCCGACCGGCCGCCACACCTT
CTTCGCCGGGATCTCGACGCTGCCGCCCGGCCATCTGCTGACGGTAAACTGGGGGCAGGGGCTGCACGTCGCCGAGCCGC
GGCCGTATCAACTACGCACCCCGCCGCCGCAGTTGCAATTGTGCGAGGACGAGGCCGAACTCGCTTTCGTCGACGCGCTG
CTGGCGGCGACGAACAGCCATCTGCAGGCCGACACCGAGGTCGTGGCGGTGCTGTCCGGCGGCATCGATTCCGCGATGCT
GGCCTGCACCGCGGCGGAACTGTCGGGCCGCAGGCTGCGCACCTTCACCGTCGCCGACACCGCCGAGCATCCCGACGCCG
TCGCCGCCGCGCGCGTGGCGCGGGCGATCGGCGCCGATCACACGCTGCTGACCTTCGGCTTCGACGATTACCTCGCGGCA
ATTCCGGCGACCATCGCGGCGGTCGAGGCGCCCGATCTGCATGCCGGGCCGTTCTTCCATCTGCTGTGCCAGGTGATCGG
CGGCTGCGCCAAAGTCTGCCTCAACGGCGAAGGCGCCGACGAGACGCTCGGCGGCTACTACGATCCGGCCGGGACGATCG
CCACGCTCACCGACGGCCAGGCGCGCGCGCAGGCCGCGGGGCTCGTTCCCAGCGACCGCGCCTGCGCCATTCGCGACGGC
CTGCTCGAAGCCGCCGCGAAAGGCGCCGCCGGCATCCGCGCCACGCTCGACATTTCGCTGCAGGATCTGCTGGAGCGCGC
CCAGCTCGATCCGGTGGATCATCTGTCGATGGCCGCGGGCGTCGAGATGCGGGTGCCCTATCTCGACCCCGTGGTGCAGG
CGGTGGTGCGGCGCGTGCCGCCGGGCGAGATCGTCGATACGTCGCTCGGCATCACCAAGCGGCTGCTGCGCCGCGCCGCG
GTGCGCCGCCACGGCGCGCATCTGCTCGACAGCGCGCTGCGCGCCAAGATGATGATGCCGCACGCCTCCGGCGGCCACGC
CGCGCGCTTTGCCCAGGCCTGCGATCACGCGGTGCCCGACGACGCGCTGCGTCGTCTGCCGTTCGGCGCGGGCTTCGCCA
GCAAGACCGATGTCGTGCTGTTCGAACTGTTCCGCCGGATCCATCTCGACGGCGGCGGCGCTGCAGTGAAAATCGACGAC
GTGTTCGCCGAGATCGCCGGATCGTCGCGCGCCGGCGCGAGCGCGATCGCGCAGGCGGCCGCGTCATGA

Upstream 100 bases:

>100_bases
ACAGCCTGACCTTGTCGCAGGCGATGGCCGAGACGGCGCGCGGCATCGCCGCGTGCCTGCGCGCATCCGCATCCACCGCA
ACGCGAACGGAGCCGCCGCG

Downstream 100 bases:

>100_bases
CCGCGGTGCTGTTCGATCTCGACGGCGTGCTGATCGATAGCTGGCAGGCGGTGGAAGCCGCCTTCCTCGCAGCCGCGGCC
GACCACGCGCTCGACGGCCG

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MCAIAGLVSLDAPPHVRPILDAIRHRGPDGLGTHRDGACEIGAARLAIVDPERGAQPMRDPLSQTAVAFNGEIYNADELR
RMLIARGHRFATRCDTEVVLRGFLEWGLDLPTHLSGMYAIAVLERGRLTLIRDPLGIKPLRYAQRGAQFAFASEAKALLR
WFGPPRLDETALADFIAIGYPTGRHTFFAGISTLPPGHLLTVNWGQGLHVAEPRPYQLRTPPPQLQLCEDEAELAFVDAL
LAATNSHLQADTEVVAVLSGGIDSAMLACTAAELSGRRLRTFTVADTAEHPDAVAAARVARAIGADHTLLTFGFDDYLAA
IPATIAAVEAPDLHAGPFFHLLCQVIGGCAKVCLNGEGADETLGGYYDPAGTIATLTDGQARAQAAGLVPSDRACAIRDG
LLEAAAKGAAGIRATLDISLQDLLERAQLDPVDHLSMAAGVEMRVPYLDPVVQAVVRRVPPGEIVDTSLGITKRLLRRAA
VRRHGAHLLDSALRAKMMMPHASGGHAARFAQACDHAVPDDALRRLPFGAGFASKTDVVLFELFRRIHLDGGGAAVKIDD
VFAEIAGSSRAGASAIAQAAAS

Sequences:

>Translated_582_residues
MCAIAGLVSLDAPPHVRPILDAIRHRGPDGLGTHRDGACEIGAARLAIVDPERGAQPMRDPLSQTAVAFNGEIYNADELR
RMLIARGHRFATRCDTEVVLRGFLEWGLDLPTHLSGMYAIAVLERGRLTLIRDPLGIKPLRYAQRGAQFAFASEAKALLR
WFGPPRLDETALADFIAIGYPTGRHTFFAGISTLPPGHLLTVNWGQGLHVAEPRPYQLRTPPPQLQLCEDEAELAFVDAL
LAATNSHLQADTEVVAVLSGGIDSAMLACTAAELSGRRLRTFTVADTAEHPDAVAAARVARAIGADHTLLTFGFDDYLAA
IPATIAAVEAPDLHAGPFFHLLCQVIGGCAKVCLNGEGADETLGGYYDPAGTIATLTDGQARAQAAGLVPSDRACAIRDG
LLEAAAKGAAGIRATLDISLQDLLERAQLDPVDHLSMAAGVEMRVPYLDPVVQAVVRRVPPGEIVDTSLGITKRLLRRAA
VRRHGAHLLDSALRAKMMMPHASGGHAARFAQACDHAVPDDALRRLPFGAGFASKTDVVLFELFRRIHLDGGGAAVKIDD
VFAEIAGSSRAGASAIAQAAAS
>Mature_582_residues
MCAIAGLVSLDAPPHVRPILDAIRHRGPDGLGTHRDGACEIGAARLAIVDPERGAQPMRDPLSQTAVAFNGEIYNADELR
RMLIARGHRFATRCDTEVVLRGFLEWGLDLPTHLSGMYAIAVLERGRLTLIRDPLGIKPLRYAQRGAQFAFASEAKALLR
WFGPPRLDETALADFIAIGYPTGRHTFFAGISTLPPGHLLTVNWGQGLHVAEPRPYQLRTPPPQLQLCEDEAELAFVDAL
LAATNSHLQADTEVVAVLSGGIDSAMLACTAAELSGRRLRTFTVADTAEHPDAVAAARVARAIGADHTLLTFGFDDYLAA
IPATIAAVEAPDLHAGPFFHLLCQVIGGCAKVCLNGEGADETLGGYYDPAGTIATLTDGQARAQAAGLVPSDRACAIRDG
LLEAAAKGAAGIRATLDISLQDLLERAQLDPVDHLSMAAGVEMRVPYLDPVVQAVVRRVPPGEIVDTSLGITKRLLRRAA
VRRHGAHLLDSALRAKMMMPHASGGHAARFAQACDHAVPDDALRRLPFGAGFASKTDVVLFELFRRIHLDGGGAAVKIDD
VFAEIAGSSRAGASAIAQAAAS

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=509, Percent_Identity=28.8801571709234, Blast_Score=122, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=486, Percent_Identity=26.1316872427983, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=486, Percent_Identity=26.5432098765432, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=336, Percent_Identity=25.5952380952381, Blast_Score=99, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=526, Percent_Identity=26.8060836501901, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=500, Percent_Identity=26.8, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45553209, Length=495, Percent_Identity=26.8686868686869, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 61696; Mature: 61696

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAIAGLVSLDAPPHVRPILDAIRHRGPDGLGTHRDGACEIGAARLAIVDPERGAQPMRD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEECCEEEEEECCCCCCCHHHH
PLSQTAVAFNGEIYNADELRRMLIARGHRFATRCDTEVVLRGFLEWGLDLPTHLSGMYAI
HHHHHHEEECCCEECHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHEEE
AVLERGRLTLIRDPLGIKPLRYAQRGAQFAFASEAKALLRWFGPPRLDETALADFIAIGY
EEECCCCEEEEECCCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PTGRHTFFAGISTLPPGHLLTVNWGQGLHVAEPRPYQLRTPPPQLQLCEDEAELAFVDAL
CCCCCEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHH
LAATNSHLQADTEVVAVLSGGIDSAMLACTAAELSGRRLRTFTVADTAEHPDAVAAARVA
HHHHCCCCCCCHHEEEEHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHH
RAIGADHTLLTFGFDDYLAAIPATIAAVEAPDLHAGPFFHLLCQVIGGCAKVCLNGEGAD
HHHCCCCEEEEECHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
ETLGGYYDPAGTIATLTDGQARAQAAGLVPSDRACAIRDGLLEAAAKGAAGIRATLDISL
CCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCH
QDLLERAQLDPVDHLSMAAGVEMRVPYLDPVVQAVVRRVPPGEIVDTSLGITKRLLRRAA
HHHHHHHCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHH
VRRHGAHLLDSALRAKMMMPHASGGHAARFAQACDHAVPDDALRRLPFGAGFASKTDVVL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHH
FELFRRIHLDGGGAAVKIDDVFAEIAGSSRAGASAIAQAAAS
HHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MCAIAGLVSLDAPPHVRPILDAIRHRGPDGLGTHRDGACEIGAARLAIVDPERGAQPMRD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEECCEEEEEECCCCCCCHHHH
PLSQTAVAFNGEIYNADELRRMLIARGHRFATRCDTEVVLRGFLEWGLDLPTHLSGMYAI
HHHHHHEEECCCEECHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHEEE
AVLERGRLTLIRDPLGIKPLRYAQRGAQFAFASEAKALLRWFGPPRLDETALADFIAIGY
EEECCCCEEEEECCCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PTGRHTFFAGISTLPPGHLLTVNWGQGLHVAEPRPYQLRTPPPQLQLCEDEAELAFVDAL
CCCCCEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHH
LAATNSHLQADTEVVAVLSGGIDSAMLACTAAELSGRRLRTFTVADTAEHPDAVAAARVA
HHHHCCCCCCCHHEEEEHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHH
RAIGADHTLLTFGFDDYLAAIPATIAAVEAPDLHAGPFFHLLCQVIGGCAKVCLNGEGAD
HHHCCCCEEEEECHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
ETLGGYYDPAGTIATLTDGQARAQAAGLVPSDRACAIRDGLLEAAAKGAAGIRATLDISL
CCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCH
QDLLERAQLDPVDHLSMAAGVEMRVPYLDPVVQAVVRRVPPGEIVDTSLGITKRLLRRAA
HHHHHHHCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHH
VRRHGAHLLDSALRAKMMMPHASGGHAARFAQACDHAVPDDALRRLPFGAGFASKTDVVL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHH
FELFRRIHLDGGGAAVKIDDVFAEIAGSSRAGASAIAQAAAS
HHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]