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The map label for this gene is ligA

Identifier: 86749131

GI number: 86749131

Start: 2283424

End: 2285568

Strand: Direct

Name: ligA

Synonym: RPB_2008

Alternate gene names: 86749131

Gene position: 2283424-2285568 (Clockwise)

Preceding gene: 86749130

Following gene: 86749134

Centisome position: 42.83

GC content: 67.13

Gene sequence:

>2145_bases
ATGACAAAAGCGCCGAAGCCCGCCCCCGACATCGCCACCCTCACCAAAGCGAAAGCCAAGGTGGAGGCGATGCGGTTGCG
GCTCGAGATCGAGCGGCACAACAGCGCGTACTACCAGCACGACGCGCCGACGGTTTCGGACGCGGAGTACGATGCGCTGC
GGCGCAGGCTGGAGGCGATCGAGGCGAAATTCCCCGAGCTCGTCAGCGCGTCGTCGCCGACACAGACGGTGGGCGCGGCG
CCGGCGCGTGGTTTCGCCAAGGTGCAGCACGCGGTGCCGATGCTGTCGCTCGGCAACGCCTTTGCCGACGACGAGGTCAC
GGAATTCGTCGAGCGCGTGCAGCGTTTCCTCCGGCTCGATGCGGTGCCGTCGATCGTCGCCGAGCCGAAGATCGACGGGC
TGTCGCTGTCGCTGCGTTACGAGCACGGCGAATTGATGCGCGCGGCGACGCGGGGCGACGGCTTCACCGGCGAGGACGTC
ACCGCCAACGTCCGCACTATCGCGGATATTCCGACCACGCTGAAGGCGAAGACGATCCCCGCCGCCTGCGAGCTGCGCGG
CGAAGTCTACATGCTGAAGCAGGATTTCCTCGCGCTCAACAAGCGGCAGGAGGAGGCCGGCGACACCGTGTTCGCCAATC
CGCGCAATTCCGCCGCCGGCTCGCTGCGGCAGAAGGATGTTGCGATCACCGCGTCGCGGCCGCTGAAATTCTTCGCCTAT
GCGTGGGGCGAGATGAGCGACTACCCGATGGACGAGCCGACCCAGTTCAAGATGCTCGGCTGGCTGAAACAGGCCGGCTT
CGTCGTCAATCCCGAGATCACGCTGTGTACCAGCGTCGACGACGCGCTCGCATTCTATCGCCGGATCGGCGAGCAGCGCG
CGGCGCTGCCCTACGACATCGACGGCGTGGTCTACAAGGTCGATCGGCTCGACTATCAGGAACGCCTCGGCTTCGTCTCG
CGCAGTCCGCGCTGGGCGATCGCGCACAAATTCGCCGCCGAGCAGGCGACCACGGTGCTGGAGAAGATCGACATCCAGGT
CGGCCGTACCGGCGCGCTCACTCCGGTGGCACGATTGCAGCCGGTGACGGTGGGTGGCGTGGTGGTGCAGAACGCGACGC
TGCACAACGAGGACTACATCAAGGGTCTCGGCAATGACGGCTCGCCGCTGCGCGACGGCGTCGACATCCGCGAGGGCGAC
ACCGTGGTGGTGCAGCGCGCCGGCGACGTGATTCCGCAGATCGTCAGCGTCGTGCTCGACAAGCGGCCGGCCGACGCGAC
GCCCTATCACTTCCCGCACAAATGCCCGGTGTGCGGCAGCCACGCGGCGCGCGAGGAGGGCGAGGCGGTGTGGCGTTGCA
CCGGCGCGCTGATCTGTCCGGCGCAGGCGGTGGAGCGGCTGAAGCATTTCGTCTCGCGGCTGGCCTTCGACATCGACGGG
CTCGGCGAGAAGCAGATCGTGCTGTTCCACGAACGCGGATGGGTGAAGGAACCCGCCGACATCTTCACGCTGCAGGCGCG
CAACGCCGCGCTGAAGCTCGAAGATATCGAAGGCTATGGCGAGACCTCGGTGCGCAATCTGTTCGCGGCGATCGACGCGC
GGCGCACCATCGAGCTGCATCGCTTGATCTTCGCGCTCGGCATCCGCCATGTCGGCGAGGGCAATGCGAAGCTGCTGGCG
CGGCACTACGGCACGCTGGATGCCTTCCTGGCGGCGATGCGCGCCGCCGCGGAAGGGCAGACTGAGGAAGGCAATACGTC
GGAGGCGTATCAGGATCTCGACAATATCGCCGGCATCGGCGAGGTCGTCGCTGCCGCTGTGGTCGAGTTCTTCGCCGAGC
CGCGCAACGTCGCGGCGCTCGATGCGCTGCTGGCCGAACTGAAGGACGTGCTGCCGGCCGAGCAGGCGCGGCGCGACACC
GCCGTCGCCGGCAAGACCGTGGTGTTCACCGGATCGCTGACGAAATTCACCCGCGACGAAGCCAAGGCGGCGGCGGAGCG
ACTGGGCGCCAAGGTCGCCGGCTCGGTGTCGAAGAAGACCGACTACGTCGTCGCCGGCGCGGACGCCGGATCGAAGCTCA
CCAAGGCGAAAGACCTCGGCGTCGCGGTGTTGACCGAGGACGAGTGGCTGGCGCTGATCTCTTGA

Upstream 100 bases:

>100_bases
GTGCAATAGCTTCGAATTCCATGCGATTGCCCTGCCGCGCGCGGGGAGTGGTGAGCCAGCCCCGCTTTCATCACCAGGTC
CTTCATTGTATCCATTGCGC

Downstream 100 bases:

>100_bases
CGCGTCATTCCGGGGCGCGAGCGCAGCTCGAGAACCCGGAATTCCGAGGTGCTCTGCCGATCGAGATTCCGGGTTCGCTC
ACTTCGTGAGCGCCCCGGAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MTKAPKPAPDIATLTKAKAKVEAMRLRLEIERHNSAYYQHDAPTVSDAEYDALRRRLEAIEAKFPELVSASSPTQTVGAA
PARGFAKVQHAVPMLSLGNAFADDEVTEFVERVQRFLRLDAVPSIVAEPKIDGLSLSLRYEHGELMRAATRGDGFTGEDV
TANVRTIADIPTTLKAKTIPAACELRGEVYMLKQDFLALNKRQEEAGDTVFANPRNSAAGSLRQKDVAITASRPLKFFAY
AWGEMSDYPMDEPTQFKMLGWLKQAGFVVNPEITLCTSVDDALAFYRRIGEQRAALPYDIDGVVYKVDRLDYQERLGFVS
RSPRWAIAHKFAAEQATTVLEKIDIQVGRTGALTPVARLQPVTVGGVVVQNATLHNEDYIKGLGNDGSPLRDGVDIREGD
TVVVQRAGDVIPQIVSVVLDKRPADATPYHFPHKCPVCGSHAAREEGEAVWRCTGALICPAQAVERLKHFVSRLAFDIDG
LGEKQIVLFHERGWVKEPADIFTLQARNAALKLEDIEGYGETSVRNLFAAIDARRTIELHRLIFALGIRHVGEGNAKLLA
RHYGTLDAFLAAMRAAAEGQTEEGNTSEAYQDLDNIAGIGEVVAAAVVEFFAEPRNVAALDALLAELKDVLPAEQARRDT
AVAGKTVVFTGSLTKFTRDEAKAAAERLGAKVAGSVSKKTDYVVAGADAGSKLTKAKDLGVAVLTEDEWLALIS

Sequences:

>Translated_714_residues
MTKAPKPAPDIATLTKAKAKVEAMRLRLEIERHNSAYYQHDAPTVSDAEYDALRRRLEAIEAKFPELVSASSPTQTVGAA
PARGFAKVQHAVPMLSLGNAFADDEVTEFVERVQRFLRLDAVPSIVAEPKIDGLSLSLRYEHGELMRAATRGDGFTGEDV
TANVRTIADIPTTLKAKTIPAACELRGEVYMLKQDFLALNKRQEEAGDTVFANPRNSAAGSLRQKDVAITASRPLKFFAY
AWGEMSDYPMDEPTQFKMLGWLKQAGFVVNPEITLCTSVDDALAFYRRIGEQRAALPYDIDGVVYKVDRLDYQERLGFVS
RSPRWAIAHKFAAEQATTVLEKIDIQVGRTGALTPVARLQPVTVGGVVVQNATLHNEDYIKGLGNDGSPLRDGVDIREGD
TVVVQRAGDVIPQIVSVVLDKRPADATPYHFPHKCPVCGSHAAREEGEAVWRCTGALICPAQAVERLKHFVSRLAFDIDG
LGEKQIVLFHERGWVKEPADIFTLQARNAALKLEDIEGYGETSVRNLFAAIDARRTIELHRLIFALGIRHVGEGNAKLLA
RHYGTLDAFLAAMRAAAEGQTEEGNTSEAYQDLDNIAGIGEVVAAAVVEFFAEPRNVAALDALLAELKDVLPAEQARRDT
AVAGKTVVFTGSLTKFTRDEAKAAAERLGAKVAGSVSKKTDYVVAGADAGSKLTKAKDLGVAVLTEDEWLALIS
>Mature_713_residues
TKAPKPAPDIATLTKAKAKVEAMRLRLEIERHNSAYYQHDAPTVSDAEYDALRRRLEAIEAKFPELVSASSPTQTVGAAP
ARGFAKVQHAVPMLSLGNAFADDEVTEFVERVQRFLRLDAVPSIVAEPKIDGLSLSLRYEHGELMRAATRGDGFTGEDVT
ANVRTIADIPTTLKAKTIPAACELRGEVYMLKQDFLALNKRQEEAGDTVFANPRNSAAGSLRQKDVAITASRPLKFFAYA
WGEMSDYPMDEPTQFKMLGWLKQAGFVVNPEITLCTSVDDALAFYRRIGEQRAALPYDIDGVVYKVDRLDYQERLGFVSR
SPRWAIAHKFAAEQATTVLEKIDIQVGRTGALTPVARLQPVTVGGVVVQNATLHNEDYIKGLGNDGSPLRDGVDIREGDT
VVVQRAGDVIPQIVSVVLDKRPADATPYHFPHKCPVCGSHAAREEGEAVWRCTGALICPAQAVERLKHFVSRLAFDIDGL
GEKQIVLFHERGWVKEPADIFTLQARNAALKLEDIEGYGETSVRNLFAAIDARRTIELHRLIFALGIRHVGEGNAKLLAR
HYGTLDAFLAAMRAAAEGQTEEGNTSEAYQDLDNIAGIGEVVAAAVVEFFAEPRNVAALDALLAELKDVLPAEQARRDTA
VAGKTVVFTGSLTKFTRDEAKAAAERLGAKVAGSVSKKTDYVVAGADAGSKLTKAKDLGVAVLTEDEWLALIS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=693, Percent_Identity=48.4848484848485, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI87082305, Length=537, Percent_Identity=25.1396648044693, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RHOP2 (Q2IYJ4)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485627.1
- ProteinModelPortal:   Q2IYJ4
- STRING:   Q2IYJ4
- GeneID:   3909514
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2008
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK589823
- BioCyc:   RPAL316058:RPB_2008-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77729; Mature: 77598

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 130-130 BINDING 128-128 BINDING 151-151 BINDING 188-188 BINDING 306-306 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAPKPAPDIATLTKAKAKVEAMRLRLEIERHNSAYYQHDAPTVSDAEYDALRRRLEAI
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHH
EAKFPELVSASSPTQTVGAAPARGFAKVQHAVPMLSLGNAFADDEVTEFVERVQRFLRLD
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHH
AVPSIVAEPKIDGLSLSLRYEHGELMRAATRGDGFTGEDVTANVRTIADIPTTLKAKTIP
CCCHHHCCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCEECCEEEECCCCCCCCCCCC
AACELRGEVYMLKQDFLALNKRQEEAGDTVFANPRNSAAGSLRQKDVAITASRPLKFFAY
HHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHCCEEEECCCCCEEEEE
AWGEMSDYPMDEPTQFKMLGWLKQAGFVVNPEITLCTSVDDALAFYRRIGEQRAALPYDI
ECCCCCCCCCCCCCHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCCC
DGVVYKVDRLDYQERLGFVSRSPRWAIAHKFAAEQATTVLEKIDIQVGRTGALTPVARLQ
CCEEEEEHHCCHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHCC
PVTVGGVVVQNATLHNEDYIKGLGNDGSPLRDGVDIREGDTVVVQRAGDVIPQIVSVVLD
CEEECCEEEECCEECCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHC
KRPADATPYHFPHKCPVCGSHAAREEGEAVWRCTGALICPAQAVERLKHFVSRLAFDIDG
CCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCC
LGEKQIVLFHERGWVKEPADIFTLQARNAALKLEDIEGYGETSVRNLFAAIDARRTIELH
CCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RLIFALGIRHVGEGNAKLLARHYGTLDAFLAAMRAAAEGQTEEGNTSEAYQDLDNIAGIG
HHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHH
EVVAAAVVEFFAEPRNVAALDALLAELKDVLPAEQARRDTAVAGKTVVFTGSLTKFTRDE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHH
AKAAAERLGAKVAGSVSKKTDYVVAGADAGSKLTKAKDLGVAVLTEDEWLALIS
HHHHHHHHCHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCEEEEECCCEEEECC
>Mature Secondary Structure 
TKAPKPAPDIATLTKAKAKVEAMRLRLEIERHNSAYYQHDAPTVSDAEYDALRRRLEAI
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHH
EAKFPELVSASSPTQTVGAAPARGFAKVQHAVPMLSLGNAFADDEVTEFVERVQRFLRLD
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHH
AVPSIVAEPKIDGLSLSLRYEHGELMRAATRGDGFTGEDVTANVRTIADIPTTLKAKTIP
CCCHHHCCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCEECCEEEECCCCCCCCCCCC
AACELRGEVYMLKQDFLALNKRQEEAGDTVFANPRNSAAGSLRQKDVAITASRPLKFFAY
HHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHCCEEEECCCCCEEEEE
AWGEMSDYPMDEPTQFKMLGWLKQAGFVVNPEITLCTSVDDALAFYRRIGEQRAALPYDI
ECCCCCCCCCCCCCHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCCC
DGVVYKVDRLDYQERLGFVSRSPRWAIAHKFAAEQATTVLEKIDIQVGRTGALTPVARLQ
CCEEEEEHHCCHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHCC
PVTVGGVVVQNATLHNEDYIKGLGNDGSPLRDGVDIREGDTVVVQRAGDVIPQIVSVVLD
CEEECCEEEECCEECCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHC
KRPADATPYHFPHKCPVCGSHAAREEGEAVWRCTGALICPAQAVERLKHFVSRLAFDIDG
CCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCC
LGEKQIVLFHERGWVKEPADIFTLQARNAALKLEDIEGYGETSVRNLFAAIDARRTIELH
CCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RLIFALGIRHVGEGNAKLLARHYGTLDAFLAAMRAAAEGQTEEGNTSEAYQDLDNIAGIG
HHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHH
EVVAAAVVEFFAEPRNVAALDALLAELKDVLPAEQARRDTAVAGKTVVFTGSLTKFTRDE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHH
AKAAAERLGAKVAGSVSKKTDYVVAGADAGSKLTKAKDLGVAVLTEDEWLALIS
HHHHHHHHCHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA