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The map label for this gene is murD

Identifier: 86749115

GI number: 86749115

Start: 2263174

End: 2264574

Strand: Direct

Name: murD

Synonym: RPB_1992

Alternate gene names: 86749115

Gene position: 2263174-2264574 (Clockwise)

Preceding gene: 86749114

Following gene: 86749116

Centisome position: 42.45

GC content: 69.16

Gene sequence:

>1401_bases
ATGATCCCCGTCACCTCTTTCGCCGGGCAATCCGTCGCGGTGTTCGGGCTCGGCGGCTCGGGGCTGGCGAGCTGCCACGC
GCTGCGCGCCGGCGGCGCCGAAGTGATCGCCTGCGACGACAATCTCGACCGCATGGTCGAAGCGGCGCAGGCCAATTTCA
TCACCGCCGATCTGCGCAATCTGCCGTGGATGAATTTTGCCGCGCTGGTGCTCACGCCGGGCGTGCCGCTGACGCATCCG
ACGCCGCATTGGAGCGTGCTCAAGGCGCGCGAGGCGGGCGTCGAGGTGATCGGTGACGTCGAGCTGTTCTGCCGCGAGCG
GCGGCTGCACGCGCCGAACGCGCCGTTCGTCGCCATCACCGGCACCAACGGCAAGTCCACCACCACGGCGCTGATCGCGC
ATCTGATGCGGCAGGCCGGCTACGACACCCAGATGGGCGGCAATATCGGCACCGCGATCCTGTCGCTGGAGCCGCCGCGC
GCCGGCCGCGTCCACGTGATCGAGATGTCGTCCTACCAGATCGATCTGACACCGTCGCTCGATCCGAGCGTCGGCATCCT
GCTCAATGTCACCGAGGACCACATCGATCGCCACGGCACCATCGAGCACTATGCCGCGGTGAAGGAGCGGCTGGTTGCCG
GCGTGCAGGACGGCGGCACCGCGATCATCGGCGTCGACGACGGCTTCGGCCGCGACGCCGCCGACCGGCTGGAGCGCGCC
GGCAAGCGCGTGGTGCGGATTTCGGTGAAGCAGCCGCTCGCCTCGGGCATCACCGCGGATCGCGAGACGATCGTGCAAGC
CGACGGCGGCGCATCGCATGAAGTCGCGAAGCTCGACGGCATCGGTTCGCTGCGCGGTTTGCACAACGCGCAGAACGCCG
CGGCGGCCGCCGCCGCAGCGCTGGCGCTCGGCGTCGGCCCGGACGTGCTGCAGAACGGCCTGCGCAGCTTCCCGGGCCTC
GCGCACCGGATGGAGCAGGTCGGACGCCAAGGCACGACGCTGTTCGTCAACGACTCCAAGGGCACCAATGCCGACGCGAC
CGCGAAAGCGCTGTCGTCGTTCGGCGAGATCTTCTGGATCGCCGGCGGCAAGCCGAAGACCGGCGGCATCGACAGCCTCG
CCGAATACTTCCCGCGCATCCGCAAGGCCTATCTGATCGGCGAGGCGGCGCAGGAATTCGCCGCGACGCTGGAAGGGCGT
GTGCCCTACGAGATCAGCGTGACGCTGGACAACGCGGTGCCGGCCGCCGCACGCGACGCCGCATCGTCGGGGCTGCCGGA
GCCGGTCGTGCTGCTGTCGCCGGCCTGCGCCTCGTTCGACCAGTTCAGGAATTTCGAAATCCGCGGGACGAAGTTCCGCG
ATCTGGTGACGGCGCTGGATGGGGTGAAGCCGGTGGCCTAG

Upstream 100 bases:

>100_bases
GGCACGCGCGGCCATCCTTCGAGGCTCGCCGAAGGCGGCGAGCACCTCAGGATGACGCCGTGTCGGTTGTGCGCGAGGTC
CTTGTGGCCGGAGCAATGCA

Downstream 100 bases:

>100_bases
CCACGGCCAACCACGTCATCGCCCGCGCAGGCGGGCGACCCAGTAGTCCGGAGCGCAGGTGCTCAGCCACGAACGCTCTG
GGATACTGGATCCCCGCCTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIPVTSFAGQSVAVFGLGGSGLASCHALRAGGAEVIACDDNLDRMVEAAQANFITADLRNLPWMNFAALVLTPGVPLTHP
TPHWSVLKAREAGVEVIGDVELFCRERRLHAPNAPFVAITGTNGKSTTTALIAHLMRQAGYDTQMGGNIGTAILSLEPPR
AGRVHVIEMSSYQIDLTPSLDPSVGILLNVTEDHIDRHGTIEHYAAVKERLVAGVQDGGTAIIGVDDGFGRDAADRLERA
GKRVVRISVKQPLASGITADRETIVQADGGASHEVAKLDGIGSLRGLHNAQNAAAAAAAALALGVGPDVLQNGLRSFPGL
AHRMEQVGRQGTTLFVNDSKGTNADATAKALSSFGEIFWIAGGKPKTGGIDSLAEYFPRIRKAYLIGEAAQEFAATLEGR
VPYEISVTLDNAVPAAARDAASSGLPEPVVLLSPACASFDQFRNFEIRGTKFRDLVTALDGVKPVA

Sequences:

>Translated_466_residues
MIPVTSFAGQSVAVFGLGGSGLASCHALRAGGAEVIACDDNLDRMVEAAQANFITADLRNLPWMNFAALVLTPGVPLTHP
TPHWSVLKAREAGVEVIGDVELFCRERRLHAPNAPFVAITGTNGKSTTTALIAHLMRQAGYDTQMGGNIGTAILSLEPPR
AGRVHVIEMSSYQIDLTPSLDPSVGILLNVTEDHIDRHGTIEHYAAVKERLVAGVQDGGTAIIGVDDGFGRDAADRLERA
GKRVVRISVKQPLASGITADRETIVQADGGASHEVAKLDGIGSLRGLHNAQNAAAAAAAALALGVGPDVLQNGLRSFPGL
AHRMEQVGRQGTTLFVNDSKGTNADATAKALSSFGEIFWIAGGKPKTGGIDSLAEYFPRIRKAYLIGEAAQEFAATLEGR
VPYEISVTLDNAVPAAARDAASSGLPEPVVLLSPACASFDQFRNFEIRGTKFRDLVTALDGVKPVA
>Mature_466_residues
MIPVTSFAGQSVAVFGLGGSGLASCHALRAGGAEVIACDDNLDRMVEAAQANFITADLRNLPWMNFAALVLTPGVPLTHP
TPHWSVLKAREAGVEVIGDVELFCRERRLHAPNAPFVAITGTNGKSTTTALIAHLMRQAGYDTQMGGNIGTAILSLEPPR
AGRVHVIEMSSYQIDLTPSLDPSVGILLNVTEDHIDRHGTIEHYAAVKERLVAGVQDGGTAIIGVDDGFGRDAADRLERA
GKRVVRISVKQPLASGITADRETIVQADGGASHEVAKLDGIGSLRGLHNAQNAAAAAAAALALGVGPDVLQNGLRSFPGL
AHRMEQVGRQGTTLFVNDSKGTNADATAKALSSFGEIFWIAGGKPKTGGIDSLAEYFPRIRKAYLIGEAAQEFAATLEGR
VPYEISVTLDNAVPAAARDAASSGLPEPVVLLSPACASFDQFRNFEIRGTKFRDLVTALDGVKPVA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=467, Percent_Identity=34.2612419700214, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_RHOP2 (Q2IYL0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485611.1
- ProteinModelPortal:   Q2IYL0
- STRING:   Q2IYL0
- GeneID:   3909498
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1992
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK01390
- BioCyc:   RPAL316058:RPB_1992-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48874; Mature: 48874

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTSFAGQSVAVFGLGGSGLASCHALRAGGAEVIACDDNLDRMVEAAQANFITADLRN
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEHHHC
LPWMNFAALVLTPGVPLTHPTPHWSVLKAREAGVEVIGDVELFCRERRLHAPNAPFVAIT
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHLMRQAGYDTQMGGNIGTAILSLEPPRAGRVHVIEMSSYQIDLTPSL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPSVGILLNVTEDHIDRHGTIEHYAAVKERLVAGVQDGGTAIIGVDDGFGRDAADRLERA
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHC
GKRVVRISVKQPLASGITADRETIVQADGGASHEVAKLDGIGSLRGLHNAQNAAAAAAAA
CCEEEEEEECCHHHHCCCCCCCEEEECCCCCCCCHHHHCCCHHHHHHCCHHHHHHHHHHH
LALGVGPDVLQNGLRSFPGLAHRMEQVGRQGTTLFVNDSKGTNADATAKALSSFGEIFWI
HHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEE
AGGKPKTGGIDSLAEYFPRIRKAYLIGEAAQEFAATLEGRVPYEISVTLDNAVPAAARDA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
ASSGLPEPVVLLSPACASFDQFRNFEIRGTKFRDLVTALDGVKPVA
HHCCCCCCEEEECCCHHCHHHHCCEEECCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIPVTSFAGQSVAVFGLGGSGLASCHALRAGGAEVIACDDNLDRMVEAAQANFITADLRN
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCEEEEHHHC
LPWMNFAALVLTPGVPLTHPTPHWSVLKAREAGVEVIGDVELFCRERRLHAPNAPFVAIT
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHLMRQAGYDTQMGGNIGTAILSLEPPRAGRVHVIEMSSYQIDLTPSL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPSVGILLNVTEDHIDRHGTIEHYAAVKERLVAGVQDGGTAIIGVDDGFGRDAADRLERA
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHC
GKRVVRISVKQPLASGITADRETIVQADGGASHEVAKLDGIGSLRGLHNAQNAAAAAAAA
CCEEEEEEECCHHHHCCCCCCCEEEECCCCCCCCHHHHCCCHHHHHHCCHHHHHHHHHHH
LALGVGPDVLQNGLRSFPGLAHRMEQVGRQGTTLFVNDSKGTNADATAKALSSFGEIFWI
HHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEE
AGGKPKTGGIDSLAEYFPRIRKAYLIGEAAQEFAATLEGRVPYEISVTLDNAVPAAARDA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
ASSGLPEPVVLLSPACASFDQFRNFEIRGTKFRDLVTALDGVKPVA
HHCCCCCCEEEECCCHHCHHHHCCEEECCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA