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The map label for this gene is nudF [H]

Identifier: 86748898

GI number: 86748898

Start: 2036487

End: 2037125

Strand: Direct

Name: nudF [H]

Synonym: RPB_1775

Alternate gene names: 86748898

Gene position: 2036487-2037125 (Clockwise)

Preceding gene: 86748897

Following gene: 86748901

Centisome position: 38.2

GC content: 71.99

Gene sequence:

>639_bases
ATGGGTTTGATCGACGGGACCGGGCTGCTCGCGGATCGCGAGGCCGATGTCACCGTGTCCGAGGCCGAGCTGATCGGCCG
CGGCTTCATGAGCTACGAGCGCTACGAGGTCTCGCTGCGGCGCGACGGCGAGCCGCCGCTGCTGCAGCGGCGCGACGTGC
TGCGCGCCGCCCGCGTCGCGGCGGTGGTGCCGATCGATCTGGCGCGCGACGCGCTGGTGCTGATCCGGCAGTTCAGGTTG
CCCGCGCATTTCGCCACCGGCCGCGGCGACATGGTGGAGATCGTCGCCGGCCGCGTCGAGTCCGGCGAGGCGCCGGCCGC
CGCGGCGCGGCGTGAGTGCATCGAAGAGATCGGCGTGGCGCCGGAACGGCTCGTCGAACTCTACAGCGTGCTGGCGACGC
CGGGCTTCACCGACGAGCAGATCGTGTATTTCGCCGGGTTCGTGGATTCCAGCGCGGCGCTGCAGCGCGGCGGCCTCGCC
GACGAGGACGAGGACACCCAGCCCTTCGTGGTGTCGATCGACGAGGCGCTGGCTGCGCTCGAGCGCGGGCAGGTCGCCAA
TGCGCTGCTGGTCGGCGCGCTGCAATGGCTGGCGCTCAACCGATCCCGGCTGCGGGAGCTGTTCGACCGAGCCGCCTGA

Upstream 100 bases:

>100_bases
CTGTTCAAACACCCGAAGACCGACTATACGCGTGCGCTGTTTGCCGCCGCGTTCCGGCTCGAGACCGCTCCGGGCGGCGG
CGCCGCGCCATAGGTGTTGG

Downstream 100 bases:

>100_bases
TCGCGGTGAGCTCGCTGCCGCTTGCTGCGATCCGCGCGATCGCCGCGTGCACAGGCTCGATTGCGGTCGCCATCGCATAC
GCATTGGCGTGGACAATGCT

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MGLIDGTGLLADREADVTVSEAELIGRGFMSYERYEVSLRRDGEPPLLQRRDVLRAARVAAVVPIDLARDALVLIRQFRL
PAHFATGRGDMVEIVAGRVESGEAPAAAARRECIEEIGVAPERLVELYSVLATPGFTDEQIVYFAGFVDSSAALQRGGLA
DEDEDTQPFVVSIDEALAALERGQVANALLVGALQWLALNRSRLRELFDRAA

Sequences:

>Translated_212_residues
MGLIDGTGLLADREADVTVSEAELIGRGFMSYERYEVSLRRDGEPPLLQRRDVLRAARVAAVVPIDLARDALVLIRQFRL
PAHFATGRGDMVEIVAGRVESGEAPAAAARRECIEEIGVAPERLVELYSVLATPGFTDEQIVYFAGFVDSSAALQRGGLA
DEDEDTQPFVVSIDEALAALERGQVANALLVGALQWLALNRSRLRELFDRAA
>Mature_211_residues
GLIDGTGLLADREADVTVSEAELIGRGFMSYERYEVSLRRDGEPPLLQRRDVLRAARVAAVVPIDLARDALVLIRQFRLP
AHFATGRGDMVEIVAGRVESGEAPAAAARRECIEEIGVAPERLVELYSVLATPGFTDEQIVYFAGFVDSSAALQRGGLAD
EDEDTQPFVVSIDEALAALERGQVANALLVGALQWLALNRSRLRELFDRAA

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=191, Percent_Identity=38.2198952879581, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788810, Length=185, Percent_Identity=27.5675675675676, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23049; Mature: 22918

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLIDGTGLLADREADVTVSEAELIGRGFMSYERYEVSLRRDGEPPLLQRRDVLRAARVA
CCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHH
AVVPIDLARDALVLIRQFRLPAHFATGRGDMVEIVAGRVESGEAPAAAARRECIEEIGVA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHCCCCCCCCCHHHHHHHHHHHHCCC
PERLVELYSVLATPGFTDEQIVYFAGFVDSSAALQRGGLADEDEDTQPFVVSIDEALAAL
HHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHH
ERGQVANALLVGALQWLALNRSRLRELFDRAA
HHCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
GLIDGTGLLADREADVTVSEAELIGRGFMSYERYEVSLRRDGEPPLLQRRDVLRAARVA
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHH
AVVPIDLARDALVLIRQFRLPAHFATGRGDMVEIVAGRVESGEAPAAAARRECIEEIGVA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHCCCCCCCCCHHHHHHHHHHHHCCC
PERLVELYSVLATPGFTDEQIVYFAGFVDSSAALQRGGLADEDEDTQPFVVSIDEALAAL
HHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHH
ERGQVANALLVGALQWLALNRSRLRELFDRAA
HHCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]