BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is braF [H]

Identifier: 86748707

GI number: 86748707

Start: 1782019

End: 1782771

Strand: Direct

Name: braF [H]

Synonym: RPB_1582

Alternate gene names: 86748707

Gene position: 1782019-1782771 (Clockwise)

Preceding gene: 86748706

Following gene: 86748708

Centisome position: 33.42

GC content: 68.53

Gene sequence:

>753_bases
ATGACGCTGGCGCTGCAGACCATCGGGCTGGACAAGCATTTCGGCGGGCTCCACGTCACCCGCAGCCTGTCGCTCAACAT
CGAGGCCGGCGCGCGCCACGCGCTGATCGGCCCCAACGGCGCCGGCAAGACCACGGTGATCAACCTGCTCACCGGCGTGC
TGAAGCCGGATGCCGGCCGCATCCTGCTGGAGGGGCAGGACATCAGCACGATGCCGGTGCACAGGCGCGTGCTGAGCGGG
CTGTCGCGCACCTTCCAGATCAATCAGCTGTATGCGGACCTGACGCCGCTCGAGACCATCGGCCTCGCGGTGTCGGAGCG
CTCCGGCCATGGCGGCGATTGGTGGCGGCGGATGGGCACCCGCGCCGACATCAACGGCGAGATCGCCGAGAATCTGGCGC
GCTTCCATCTGCTCGACGTCATGAACGAGCCGACCGGGCGGCTGCCTTATGGCAAGCAGCGGCTGCTGGAGATCGCGGTC
GCGATCGCCACCAAGCCGCGCGTGCTGCTGCTCGACGAACCCGCCGCCGGCGTGCCCGAAAGCGAGCGCCACGACATTCT
CGCCGTGGTGGCGAACCTGCCGCGCGACGTCACCGTGCTGTTGATCGAGCACGACATGGATCTGGTGTTCTCCTTCGCCG
ACCGGATCTCGGTGCTGGTGTCGGGCGCATTGCTGGTCGAAGGCCCGCCCGCCGAGGTCGCGCGCGATCCGCAGGTCAAG
GCGGTGTATCTCGGCGAGGACGCCCATGCCTGA

Upstream 100 bases:

>100_bases
TCGTCCTGATCGGTCGCGAGCGGCTGCAGAACTGGGTGCTGTTCGTGCCGCGCCAAGTGATGCGCGCGCTGCGCCGCGCC
GGCCCGAGCGGAGGCGCGAC

Downstream 100 bases:

>100_bases
GCTTCCAATGCTTGAGCTTCCAAATGTCTGAGCTGCTGGCGATCGACAGGCTGCGCGCCGGCTATGGCGAGGCCGTGGTG
CTGCCGGAGCTGTCGCTGCA

Product: ABC transporter related

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTLALQTIGLDKHFGGLHVTRSLSLNIEAGARHALIGPNGAGKTTVINLLTGVLKPDAGRILLEGQDISTMPVHRRVLSG
LSRTFQINQLYADLTPLETIGLAVSERSGHGGDWWRRMGTRADINGEIAENLARFHLLDVMNEPTGRLPYGKQRLLEIAV
AIATKPRVLLLDEPAAGVPESERHDILAVVANLPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGPPAEVARDPQVK
AVYLGEDAHA

Sequences:

>Translated_250_residues
MTLALQTIGLDKHFGGLHVTRSLSLNIEAGARHALIGPNGAGKTTVINLLTGVLKPDAGRILLEGQDISTMPVHRRVLSG
LSRTFQINQLYADLTPLETIGLAVSERSGHGGDWWRRMGTRADINGEIAENLARFHLLDVMNEPTGRLPYGKQRLLEIAV
AIATKPRVLLLDEPAAGVPESERHDILAVVANLPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGPPAEVARDPQVK
AVYLGEDAHA
>Mature_249_residues
TLALQTIGLDKHFGGLHVTRSLSLNIEAGARHALIGPNGAGKTTVINLLTGVLKPDAGRILLEGQDISTMPVHRRVLSGL
SRTFQINQLYADLTPLETIGLAVSERSGHGGDWWRRMGTRADINGEIAENLARFHLLDVMNEPTGRLPYGKQRLLEIAVA
IATKPRVLLLDEPAAGVPESERHDILAVVANLPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGPPAEVARDPQVKA
VYLGEDAHA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=230, Percent_Identity=32.1739130434783, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI30795238, Length=230, Percent_Identity=32.1739130434783, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI31657092, Length=212, Percent_Identity=29.7169811320755, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI116734710, Length=208, Percent_Identity=31.25, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI27436953, Length=208, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI27262624, Length=211, Percent_Identity=29.3838862559242, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI27262626, Length=211, Percent_Identity=29.3838862559242, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI153792144, Length=208, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI105990541, Length=197, Percent_Identity=31.4720812182741, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI150417984, Length=230, Percent_Identity=31.304347826087, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI6005701, Length=209, Percent_Identity=31.1004784688995, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI45446740, Length=210, Percent_Identity=29.5238095238095, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI47078218, Length=210, Percent_Identity=29.5238095238095, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27477115, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI11967969, Length=225, Percent_Identity=25.7777777777778, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI255708477, Length=223, Percent_Identity=26.9058295964126, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI9961250, Length=241, Percent_Identity=23.2365145228216, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI4505771, Length=241, Percent_Identity=23.2365145228216, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI9961252, Length=241, Percent_Identity=23.2365145228216, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1789864, Length=247, Percent_Identity=38.8663967611336, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789593, Length=245, Percent_Identity=33.0612244897959, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787370, Length=246, Percent_Identity=29.2682926829268, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081791, Length=242, Percent_Identity=28.099173553719, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI87081709, Length=240, Percent_Identity=27.0833333333333, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788472, Length=239, Percent_Identity=27.1966527196653, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1790190, Length=241, Percent_Identity=28.2157676348548, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI48995001, Length=243, Percent_Identity=29.2181069958848, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786319, Length=228, Percent_Identity=29.8245614035088, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789672, Length=240, Percent_Identity=26.25, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788761, Length=250, Percent_Identity=26.8, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081782, Length=235, Percent_Identity=30.6382978723404, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788210, Length=215, Percent_Identity=27.4418604651163, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790525, Length=236, Percent_Identity=27.1186440677966, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786253, Length=230, Percent_Identity=27.8260869565217, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994943, Length=234, Percent_Identity=30.3418803418803, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787029, Length=231, Percent_Identity=25.5411255411255, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87082268, Length=234, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789873, Length=218, Percent_Identity=29.3577981651376, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787548, Length=225, Percent_Identity=26.2222222222222, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790739, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787792, Length=236, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786398, Length=221, Percent_Identity=26.6968325791855, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786345, Length=250, Percent_Identity=29.2, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789991, Length=225, Percent_Identity=28.4444444444444, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790162, Length=230, Percent_Identity=25.6521739130435, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693107, Length=231, Percent_Identity=29.4372294372294, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788165, Length=234, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787712, Length=244, Percent_Identity=23.3606557377049, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788450, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786803, Length=237, Percent_Identity=27.0042194092827, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788897, Length=222, Percent_Identity=27.4774774774775, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789032, Length=243, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790467, Length=236, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788225, Length=243, Percent_Identity=25.5144032921811, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789962, Length=256, Percent_Identity=24.21875, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787758, Length=234, Percent_Identity=25.6410256410256, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082267, Length=229, Percent_Identity=25.764192139738, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787089, Length=246, Percent_Identity=23.9837398373984, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787759, Length=232, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081834, Length=206, Percent_Identity=25.7281553398058, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787105, Length=221, Percent_Identity=28.0542986425339, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788644, Length=255, Percent_Identity=23.921568627451, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1787164, Length=217, Percent_Identity=26.7281105990783, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=213, Percent_Identity=30.0469483568075, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565586, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=220, Percent_Identity=30.4545454545455, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=214, Percent_Identity=29.4392523364486, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=214, Percent_Identity=29.4392523364486, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=214, Percent_Identity=29.4392523364486, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533592, Length=200, Percent_Identity=29.5, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=216, Percent_Identity=28.7037037037037, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322980, Length=224, Percent_Identity=24.5535714285714, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643674, Length=227, Percent_Identity=30.8370044052863, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI221512771, Length=226, Percent_Identity=29.2035398230088, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24666092, Length=221, Percent_Identity=28.9592760180996, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI221500365, Length=220, Percent_Identity=28.1818181818182, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI116007184, Length=220, Percent_Identity=28.1818181818182, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574150, Length=225, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI116007328, Length=225, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28571209, Length=224, Percent_Identity=24.5535714285714, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28573571, Length=227, Percent_Identity=24.2290748898678, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19920532, Length=226, Percent_Identity=26.5486725663717, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24580930, Length=226, Percent_Identity=26.5486725663717, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28573573, Length=220, Percent_Identity=24.5454545454545, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI85724858, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI85724856, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580547, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580551, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580549, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580545, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27010; Mature: 26878

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLALQTIGLDKHFGGLHVTRSLSLNIEAGARHALIGPNGAGKTTVINLLTGVLKPDAGR
CEEEEEEECCCCCCCCEEEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCE
ILLEGQDISTMPVHRRVLSGLSRTFQINQLYADLTPLETIGLAVSERSGHGGDWWRRMGT
EEEECCCCCCCHHHHHHHHHHHHEEEHHHHHHCCCCHHHHCEEEECCCCCCHHHHHHCCC
RADINGEIAENLARFHLLDVMNEPTGRLPYGKQRLLEIAVAIATKPRVLLLDEPAAGVPE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
SERHDILAVVANLPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGPPAEVARDPQVK
CCCCHHHEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCEE
AVYLGEDAHA
EEEECCCCCC
>Mature Secondary Structure 
TLALQTIGLDKHFGGLHVTRSLSLNIEAGARHALIGPNGAGKTTVINLLTGVLKPDAGR
EEEEEEECCCCCCCCEEEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCE
ILLEGQDISTMPVHRRVLSGLSRTFQINQLYADLTPLETIGLAVSERSGHGGDWWRRMGT
EEEECCCCCCCHHHHHHHHHHHHEEEHHHHHHCCCCHHHHCEEEECCCCCCHHHHHHCCC
RADINGEIAENLARFHLLDVMNEPTGRLPYGKQRLLEIAVAIATKPRVLLLDEPAAGVPE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
SERHDILAVVANLPRDVTVLLIEHDMDLVFSFADRISVLVSGALLVEGPPAEVARDPQVK
CCCCHHHEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCEE
AVYLGEDAHA
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]