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The map label for this gene is braE [H]

Identifier: 86748706

GI number: 86748706

Start: 1781003

End: 1782022

Strand: Direct

Name: braE [H]

Synonym: RPB_1581

Alternate gene names: 86748706

Gene position: 1781003-1782022 (Clockwise)

Preceding gene: 86748705

Following gene: 86748707

Centisome position: 33.4

GC content: 66.86

Gene sequence:

>1020_bases
ATGACCGCGCCCGCAGATAATGTCGGCTTTCACGCCCGCCGCACCGCTCGCTGGCATGCCGGCGAGTTCGTGTTCTGGAT
CGCGCTGATCGGCTGCGGCCTGCTGTTTCCGTCGCGCTATCTGATCATGACCGAGATCCTCCGGATCGGCCTGTTCGCGC
TGTCGCTCGACCTGATCCTCGGCTATGCCGGCATCGTTTCGCTCGGCCACGCCGCGTTCTTCGGCGTCGGCGCCTATGCG
GCCGGCCTGATGGCGCTGCACGGCATCGTGGGCGAGCCGGTGCTGGCGCTGTTGGTCGCCGGGCTGGTGGCTGCCGTGCT
CGGCTTCGCCACCAGCTTCCTGGTGATCCGCGGCGTCGATCTCACCCGGCTGATGGTGACACTCGGCATCGCGCTCTTGA
TGGAAGCGCTGGCGGAGCGGTTCTCCGACATCACCGGCGGCTCCGACGGCCTGCAGGGCATCGTGATGCAGCCGATCCTC
GGCCTGTTCGAGTTCGACATCTTCGGCAAGGTCGCGTTCTGGTACAGCGTCGCGGTGCTGTTCGTGCTGTTCCTCTTGGC
GCGCCGCGTCGTGCATTCGCCGTTCGGGCTCAGCCTGCGCGCGATCCGCAACAATCCGCTGCGCGCCTCCGCGGTCGGCA
TTCCGGTGAACCGGCGGCTGGTGATGGTCTACACGCTCGCCGCATTCTATGCCGGCATCGCCGGCGCGCTGTTCACCCAG
ACCACGCAACTCGCCTCGCTCGACGTGTTTTCGTTCGAGCGCTCGGCCGATCTGGTGCTGGTGCTGGTGCTCGGCGGCAC
CGGCTATCTCTATGGCGGGCTGATCGGCGCCGTGATCTTCAAGATCCTGCAGGAGTTCTTCTCCACGCTCACGCCGCAAT
ACTGGCTGTTCTGGATCGGCCTGGTGCTGGTGACGATCGTCCTGATCGGTCGCGAGCGGCTGCAGAACTGGGTGCTGTTC
GTGCCGCGCCAAGTGATGCGCGCGCTGCGCCGCGCCGGCCCGAGCGGAGGCGCGACATGA

Upstream 100 bases:

>100_bases
TTTCCCAAGCTCGGCCCGTTCGTGATCTACACCATCATGATCGTGATCCTGATCTGGCGCCCCAACGGCCTGTTCGGCCG
CACCGCGGCGCGGTGACGGC

Downstream 100 bases:

>100_bases
CGCTGGCGCTGCAGACCATCGGGCTGGACAAGCATTTCGGCGGGCTCCACGTCACCCGCAGCCTGTCGCTCAACATCGAG
GCCGGCGCGCGCCACGCGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTAPADNVGFHARRTARWHAGEFVFWIALIGCGLLFPSRYLIMTEILRIGLFALSLDLILGYAGIVSLGHAAFFGVGAYA
AGLMALHGIVGEPVLALLVAGLVAAVLGFATSFLVIRGVDLTRLMVTLGIALLMEALAERFSDITGGSDGLQGIVMQPIL
GLFEFDIFGKVAFWYSVAVLFVLFLLARRVVHSPFGLSLRAIRNNPLRASAVGIPVNRRLVMVYTLAAFYAGIAGALFTQ
TTQLASLDVFSFERSADLVLVLVLGGTGYLYGGLIGAVIFKILQEFFSTLTPQYWLFWIGLVLVTIVLIGRERLQNWVLF
VPRQVMRALRRAGPSGGAT

Sequences:

>Translated_339_residues
MTAPADNVGFHARRTARWHAGEFVFWIALIGCGLLFPSRYLIMTEILRIGLFALSLDLILGYAGIVSLGHAAFFGVGAYA
AGLMALHGIVGEPVLALLVAGLVAAVLGFATSFLVIRGVDLTRLMVTLGIALLMEALAERFSDITGGSDGLQGIVMQPIL
GLFEFDIFGKVAFWYSVAVLFVLFLLARRVVHSPFGLSLRAIRNNPLRASAVGIPVNRRLVMVYTLAAFYAGIAGALFTQ
TTQLASLDVFSFERSADLVLVLVLGGTGYLYGGLIGAVIFKILQEFFSTLTPQYWLFWIGLVLVTIVLIGRERLQNWVLF
VPRQVMRALRRAGPSGGAT
>Mature_338_residues
TAPADNVGFHARRTARWHAGEFVFWIALIGCGLLFPSRYLIMTEILRIGLFALSLDLILGYAGIVSLGHAAFFGVGAYAA
GLMALHGIVGEPVLALLVAGLVAAVLGFATSFLVIRGVDLTRLMVTLGIALLMEALAERFSDITGGSDGLQGIVMQPILG
LFEFDIFGKVAFWYSVAVLFVLFLLARRVVHSPFGLSLRAIRNNPLRASAVGIPVNRRLVMVYTLAAFYAGIAGALFTQT
TQLASLDVFSFERSADLVLVLVLGGTGYLYGGLIGAVIFKILQEFFSTLTPQYWLFWIGLVLVTIVLIGRERLQNWVLFV
PRQVMRALRRAGPSGGAT

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=280, Percent_Identity=26.0714285714286, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36672; Mature: 36541

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPADNVGFHARRTARWHAGEFVFWIALIGCGLLFPSRYLIMTEILRIGLFALSLDLIL
CCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYAGIVSLGHAAFFGVGAYAAGLMALHGIVGEPVLALLVAGLVAAVLGFATSFLVIRGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LTRLMVTLGIALLMEALAERFSDITGGSDGLQGIVMQPILGLFEFDIFGKVAFWYSVAVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVLFLLARRVVHSPFGLSLRAIRNNPLRASAVGIPVNRRLVMVYTLAAFYAGIAGALFTQ
HHHHHHHHHHHCCCCCCEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
TTQLASLDVFSFERSADLVLVLVLGGTGYLYGGLIGAVIFKILQEFFSTLTPQYWLFWIG
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LVLVTIVLIGRERLQNWVLFVPRQVMRALRRAGPSGGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TAPADNVGFHARRTARWHAGEFVFWIALIGCGLLFPSRYLIMTEILRIGLFALSLDLIL
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]