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The map label for this gene is gca [H]

Identifier: 86748677

GI number: 86748677

Start: 1749350

End: 1750324

Strand: Reverse

Name: gca [H]

Synonym: RPB_1552

Alternate gene names: 86748677

Gene position: 1750324-1749350 (Counterclockwise)

Preceding gene: 86748680

Following gene: 86748667

Centisome position: 32.83

GC content: 62.05

Gene sequence:

>975_bases
ATGGCCAAGACCGCACTGATATTCGGGATCTCCGGACAGGATGGCGCCCTGCTTGCGCTGCATCTGCTGGAGCGCGGATA
CGAGGTCCATGGCACGTCGCGTGACAAGGAATTGTCGAGCTTCGCGAACCTCAGGCGTGTCGGAATTCAGGATCGGGTTC
GGCTGTATTCCGCCAGCCTCACGGACTTCCGCAGCGTGCTGCAGGTCGTCGCCGACGCCGCCCCCAGCGAGATCTACAAC
CTGGCGGGGCAATCGTCGGTCAGCCTGTCGTTCGAACAGCCGGTCGAAACCCTGGATTCCACGATCTTCGGGCCCCTCAA
CATTCTCGAATCGATCCGGCTTCTGAAGCTCGATGCGCGGCTCTACAACGCCTGCTCGAGTGAGTGTTTCGGCAATACCG
AGCTGGCGGCGACCGAACAGACCCCGTTCAATCCGCGAAGTCCCTACGGCGTCGGCAAGGCGGCCGCATTCTGGACCGTC
GCCAATTATCGCGATGCTTACGGCTTGTTCGCCTGCTCCGGCATCCTGTTCAATCACGACTCGCCGTTGCGTCCCCGTCG
GTTCGTCACGCGCAAGATCGTCGCCGGCGCGGCCGATATCGCCGAGAAGAAATCCGACCGTCTGATGCTCGGCAGCCTGC
ATGTCGCGCGCGACTTCGGCTGGGCGCCGGAATACGTCGAAGCAATGGCGATGATGCTGGGCGCGGACAGCCCCGGAGAC
TACGTCATCGCAACGGGACAAACGCACTCGCTCGAGAGCTTTGTCGACAAGGCGTTTGCATTTTTCGGTCTGGATTGGCG
CCGCCATGTCGAGATCGACGATCGCCTGATGCGTCCGACGGAAATCGTGACCAGCGCGGGAAACCCCGAGAAGGCCAAGC
GGGTGCTGGGCTGGGAAGCAACCGTGACCATGGAAGAGGTGGTGCGTAGACTTTCGGAAGCCGAGTTGAGTCTACGGCAC
GACGCTTCCGCCTGA

Upstream 100 bases:

>100_bases
GCAACCGGCTCCGCGCCCCGCCGGGCGACCGGAACGCCCGTATTTTGCAGCGGGCGGCGCTTCCGGTACAGTCGCGCGCT
GACATTCAGGATTCGAGTTC

Downstream 100 bases:

>100_bases
GCGCTCCGACAGAAACGCCGCGGCCGTTCTGCGCAGCCCTTCGTCGACCGAGACCGGCGGCGTCCATTCCAGAAGCTGCC
GGGCCTTGCCGATCTCCAGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAKTALIFGISGQDGALLALHLLERGYEVHGTSRDKELSSFANLRRVGIQDRVRLYSASLTDFRSVLQVVADAAPSEIYN
LAGQSSVSLSFEQPVETLDSTIFGPLNILESIRLLKLDARLYNACSSECFGNTELAATEQTPFNPRSPYGVGKAAAFWTV
ANYRDAYGLFACSGILFNHDSPLRPRRFVTRKIVAGAADIAEKKSDRLMLGSLHVARDFGWAPEYVEAMAMMLGADSPGD
YVIATGQTHSLESFVDKAFAFFGLDWRRHVEIDDRLMRPTEIVTSAGNPEKAKRVLGWEATVTMEEVVRRLSEAELSLRH
DASA

Sequences:

>Translated_324_residues
MAKTALIFGISGQDGALLALHLLERGYEVHGTSRDKELSSFANLRRVGIQDRVRLYSASLTDFRSVLQVVADAAPSEIYN
LAGQSSVSLSFEQPVETLDSTIFGPLNILESIRLLKLDARLYNACSSECFGNTELAATEQTPFNPRSPYGVGKAAAFWTV
ANYRDAYGLFACSGILFNHDSPLRPRRFVTRKIVAGAADIAEKKSDRLMLGSLHVARDFGWAPEYVEAMAMMLGADSPGD
YVIATGQTHSLESFVDKAFAFFGLDWRRHVEIDDRLMRPTEIVTSAGNPEKAKRVLGWEATVTMEEVVRRLSEAELSLRH
DASA
>Mature_323_residues
AKTALIFGISGQDGALLALHLLERGYEVHGTSRDKELSSFANLRRVGIQDRVRLYSASLTDFRSVLQVVADAAPSEIYNL
AGQSSVSLSFEQPVETLDSTIFGPLNILESIRLLKLDARLYNACSSECFGNTELAATEQTPFNPRSPYGVGKAAAFWTVA
NYRDAYGLFACSGILFNHDSPLRPRRFVTRKIVAGAADIAEKKSDRLMLGSLHVARDFGWAPEYVEAMAMMLGADSPGDY
VIATGQTHSLESFVDKAFAFFGLDWRRHVEIDDRLMRPTEIVTSAGNPEKAKRVLGWEATVTMEEVVRRLSEAELSLRHD
ASA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=40.9356725146199, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=40.1129943502825, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=41.5697674418605, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=41.5697674418605, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=41.5697674418605, Blast_Score=252, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=41.5697674418605, Blast_Score=252, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=41.5697674418605, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=40.406976744186, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24158427, Length=344, Percent_Identity=41.5697674418605, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 35702; Mature: 35571

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTALIFGISGQDGALLALHLLERGYEVHGTSRDKELSSFANLRRVGIQDRVRLYSASL
CCCEEEEEEECCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
TDFRSVLQVVADAAPSEIYNLAGQSSVSLSFEQPVETLDSTIFGPLNILESIRLLKLDAR
HHHHHHHHHHHCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LYNACSSECFGNTELAATEQTPFNPRSPYGVGKAAAFWTVANYRDAYGLFACSGILFNHD
HHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCEEECCC
SPLRPRRFVTRKIVAGAADIAEKKSDRLMLGSLHVARDFGWAPEYVEAMAMMLGADSPGD
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
YVIATGQTHSLESFVDKAFAFFGLDWRRHVEIDDRLMRPTEIVTSAGNPEKAKRVLGWEA
EEEECCCCCCHHHHHHHHHHHHCCCCCEECCHHHHHCCCHHHHHCCCCHHHHHHHHCCCC
TVTMEEVVRRLSEAELSLRHDASA
EEEHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKTALIFGISGQDGALLALHLLERGYEVHGTSRDKELSSFANLRRVGIQDRVRLYSASL
CCEEEEEEECCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
TDFRSVLQVVADAAPSEIYNLAGQSSVSLSFEQPVETLDSTIFGPLNILESIRLLKLDAR
HHHHHHHHHHHCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LYNACSSECFGNTELAATEQTPFNPRSPYGVGKAAAFWTVANYRDAYGLFACSGILFNHD
HHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCEEECCC
SPLRPRRFVTRKIVAGAADIAEKKSDRLMLGSLHVARDFGWAPEYVEAMAMMLGADSPGD
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
YVIATGQTHSLESFVDKAFAFFGLDWRRHVEIDDRLMRPTEIVTSAGNPEKAKRVLGWEA
EEEECCCCCCHHHHHHHHHHHHCCCCCEECCHHHHHCCCHHHHHCCCCHHHHHHHHCCCC
TVTMEEVVRRLSEAELSLRHDASA
EEEHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]