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The map label for this gene is noeL [H]

Identifier: 86748650

GI number: 86748650

Start: 1718054

End: 1719136

Strand: Direct

Name: noeL [H]

Synonym: RPB_1525

Alternate gene names: 86748650

Gene position: 1718054-1719136 (Clockwise)

Preceding gene: 86748647

Following gene: 86748651

Centisome position: 32.22

GC content: 58.54

Gene sequence:

>1083_bases
TTGGCTAATCCGAGTTCGAAACGAGTTGCGCTCATCACTGGGGTCACGGGGCAGGACGGCGCATATCTTGCCGAATACCT
GCTGGGGCTCGGTTACACCGTCCACGGGGTCAAGCGTCGATCATCCTCCTTCAACACCGGGCGGATCGATCATCTTTACC
AGGACCGTCACGCGGCCAATGTGCCGTTCCTCCTCCACTACGGAGACATGACCGACGCCACCAATCTGATCCGTCTGATT
CAGGAAATTCAGCCAGACGAAATCTACAACCTGGCGGCGCAGAGTCATGTTGCGGTGAGTTTCGAGAGCCCGGAGTATAC
CGCCAATTCCGACGCAATCGGCGTCCTGCGCTTGCTGGAGGCGATCCGGATTCTCGGGATGGAAAAGAAGACGCGCTTCT
ACCAGGCGTCCACGTCCGAGCTCTACGGGCTCGTCCAGCAAATTCCTCAGACGGAGAAGACGCCGTTTTATCCGCGCTCG
CCCTATGCGGTCGCGAAATTATACGGCTACTGGATCACGGTCAATTATCGAGAAGCCTACGGAATGTTCGCCTCGAACGG
CATCTTGTTCAATCACGAGAGCCCGATCCGGGGCGAAACATTCGTGACGCGCAAGATCACGCGGTCGATCGCCCGCATCG
AACTGGGTGTCGAAAATTGTCTGTATCTCGGAAACCTCGATGCGAAGCGCGATTGGGGACATGCCAAAGACTATATCGAG
GGCATGCACAAGATTCTCCAGGCGAGCGAGCCGGACGATTTCGTGCTGGCGACGGGCGAGACCCGGTCGGTGCGCGAATT
CGTCGAAGTGGCGTTCTCGGAACTGGGCCGCAGCATCGAATGGCAGGGCCAGGGGTTGGACGAGATCGGCATCGACAAGG
CGTCGAACAAAGTGGTCGTTCGGATCGACGCGAATTATTTCCGGCCCACCGAGGTCGAGCTGCTGATCGGCGATGCCACC
AAGGCTCGTGAGAAACTCGGCTGGACGCCGAAGACGACTTTCGCCGAGCTGGTGAAGGAGATGGTGGCGAGCGATCTGGC
GATCGCCCGACGAGAGGCCGAGAATGGCAAATCTGCCGTTTGA

Upstream 100 bases:

>100_bases
TTGATCGAATGGGGGCATGCTAAGTCTTCGGCCTTCTTTCCAGAGAGTGGTCTCTGGCGGGCGGCTTGCAGCCCCTCGCG
TCGGCGCAAGAGGAGTTAAT

Downstream 100 bases:

>100_bases
GCTGAAGGGCAAGCGGATCTACGTCGCTGGTCACGCCGGCATGGTCGGGGGCGCTTTGATGCGTCGTCTCGAGCAGGAAG
ACGTGGAAACGCTCGTCGCG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MANPSSKRVALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTGRIDHLYQDRHAANVPFLLHYGDMTDATNLIRLI
QEIQPDEIYNLAAQSHVAVSFESPEYTANSDAIGVLRLLEAIRILGMEKKTRFYQASTSELYGLVQQIPQTEKTPFYPRS
PYAVAKLYGYWITVNYREAYGMFASNGILFNHESPIRGETFVTRKITRSIARIELGVENCLYLGNLDAKRDWGHAKDYIE
GMHKILQASEPDDFVLATGETRSVREFVEVAFSELGRSIEWQGQGLDEIGIDKASNKVVVRIDANYFRPTEVELLIGDAT
KAREKLGWTPKTTFAELVKEMVASDLAIARREAENGKSAV

Sequences:

>Translated_360_residues
MANPSSKRVALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTGRIDHLYQDRHAANVPFLLHYGDMTDATNLIRLI
QEIQPDEIYNLAAQSHVAVSFESPEYTANSDAIGVLRLLEAIRILGMEKKTRFYQASTSELYGLVQQIPQTEKTPFYPRS
PYAVAKLYGYWITVNYREAYGMFASNGILFNHESPIRGETFVTRKITRSIARIELGVENCLYLGNLDAKRDWGHAKDYIE
GMHKILQASEPDDFVLATGETRSVREFVEVAFSELGRSIEWQGQGLDEIGIDKASNKVVVRIDANYFRPTEVELLIGDAT
KAREKLGWTPKTTFAELVKEMVASDLAIARREAENGKSAV
>Mature_359_residues
ANPSSKRVALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTGRIDHLYQDRHAANVPFLLHYGDMTDATNLIRLIQ
EIQPDEIYNLAAQSHVAVSFESPEYTANSDAIGVLRLLEAIRILGMEKKTRFYQASTSELYGLVQQIPQTEKTPFYPRSP
YAVAKLYGYWITVNYREAYGMFASNGILFNHESPIRGETFVTRKITRSIARIELGVENCLYLGNLDAKRDWGHAKDYIEG
MHKILQASEPDDFVLATGETRSVREFVEVAFSELGRSIEWQGQGLDEIGIDKASNKVVVRIDANYFRPTEVELLIGDATK
AREKLGWTPKTTFAELVKEMVASDLAIARREAENGKSAV

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=353, Percent_Identity=60.9065155807365, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=66.0112359550562, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=26.4204545454545, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788353, Length=296, Percent_Identity=25.6756756756757, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=63.1123919308357, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=63.1123919308357, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI133901790, Length=347, Percent_Identity=63.1123919308357, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=63.1123919308357, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=63.1123919308357, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=64.8255813953488, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=61.7816091954023, Blast_Score=462, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 40343; Mature: 40212

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPSSKRVALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTGRIDHLYQDRHAAN
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHHHHCCC
VPFLLHYGDMTDATNLIRLIQEIQPDEIYNLAAQSHVAVSFESPEYTANSDAIGVLRLLE
CCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHH
AIRILGMEKKTRFYQASTSELYGLVQQIPQTEKTPFYPRSPYAVAKLYGYWITVNYREAY
HHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHH
GMFASNGILFNHESPIRGETFVTRKITRSIARIELGVENCLYLGNLDAKRDWGHAKDYIE
CEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCHHHHHHH
GMHKILQASEPDDFVLATGETRSVREFVEVAFSELGRSIEWQGQGLDEIGIDKASNKVVV
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEE
RIDANYFRPTEVELLIGDATKAREKLGWTPKTTFAELVKEMVASDLAIARREAENGKSAV
EEECCCCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANPSSKRVALITGVTGQDGAYLAEYLLGLGYTVHGVKRRSSSFNTGRIDHLYQDRHAAN
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHHHHCCC
VPFLLHYGDMTDATNLIRLIQEIQPDEIYNLAAQSHVAVSFESPEYTANSDAIGVLRLLE
CCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHH
AIRILGMEKKTRFYQASTSELYGLVQQIPQTEKTPFYPRSPYAVAKLYGYWITVNYREAY
HHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHH
GMFASNGILFNHESPIRGETFVTRKITRSIARIELGVENCLYLGNLDAKRDWGHAKDYIE
CEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCHHHHHHH
GMHKILQASEPDDFVLATGETRSVREFVEVAFSELGRSIEWQGQGLDEIGIDKASNKVVV
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEE
RIDANYFRPTEVELLIGDATKAREKLGWTPKTTFAELVKEMVASDLAIARREAENGKSAV
EEECCCCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]