Click here to switch to the map view.

The map label for this gene is fcl [C]

Identifier: 86748651

GI number: 86748651

Start: 1719117

End: 1720070

Strand: Direct

Name: fcl [C]

Synonym: RPB_1526

Alternate gene names: 86748651

Gene position: 1719117-1720070 (Clockwise)

Preceding gene: 86748650

Following gene: 86748652

Centisome position: 32.24

GC content: 60.48

Gene sequence:

>954_bases
ATGGCAAATCTGCCGTTTGAGCTGAAGGGCAAGCGGATCTACGTCGCTGGTCACGCCGGCATGGTCGGGGGCGCTTTGAT
GCGTCGTCTCGAGCAGGAAGACGTGGAAACGCTCGTCGCGGCGAGGGCCGAACTGGATTTGCGGGATCAAGCGGCGGTCA
ATGGCTGGTTCGCGAGCCATCGTCCCCAGGTCGTGTTCCTGGCCGCGGCGAAAGTCGGCGGCATTGCCGCCAACGACGCG
CTGCGCGGCGAATTCATCTACGACAACATCATGATCGCAGCCAACGTGATCCACGCTGCGCACCGCGTCGGCGTCGAGAA
GCTGATGTGTCTCGGGTCATCCTGCATCTTTCCGAAGTTTGCGCCGCAGCCACTTCGAGAGGAGGCGCTGCTGACGGGAC
TGCTGGAGCCGACCAACGAACCCTATGCGATTGCCAAGATTGCCGCGATCAAGCTGGTCGAGGCCTATCGAAGTCAGTTC
GGCTCCGATTTCATCAACGTCATGCCGACCAACGTGTACGGTCCGGGGGATAATTTTCATCCCGAATACAGTCATGTCGT
GGCTGCATTGATCCGACGCTTTCACGAAGCAAAGATGGCCGATGCCACCAACGTGCTGGTCTGGGGATCGGGAACGCCGC
GTCGCGAGTTCCTGTACGTCGACGATCTCGCGGACGCCTGTGTGCACCTCATGAGGAATTACTCCGATCCACAGTTCATC
AATGTCGGAACCGGAACCGATCTGACGATCGCCGAATTGGCGAAGGTCATCGCATCGGTGGTCGGGTATGCCGGTGAGAT
CAGCTTCGATTCCTCTCGTCCGGATGGCACCCCACGGAAGTTGCTCGATGTCAGCCGGCTCACCGGCCTGGGTTGGCGCG
CGCGGACGTCACTGCAAGACGGAATTCGACTGGCCTACGAGGCGTTCATCGATCGGCCTGCGAGCAGGGCATGA

Upstream 100 bases:

>100_bases
GCTCGTGAGAAACTCGGCTGGACGCCGAAGACGACTTTCGCCGAGCTGGTGAAGGAGATGGTGGCGAGCGATCTGGCGAT
CGCCCGACGAGAGGCCGAGA

Downstream 100 bases:

>100_bases
CCGGATCTCCGGATCGTTCGCCCGATCGGCTGGTGGTGTGAGCATGACGTTGCGAAGGCCTAATCCGGGATCGGGAACGC
GCATTCGTCGAACCGCGCCG

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MANLPFELKGKRIYVAGHAGMVGGALMRRLEQEDVETLVAARAELDLRDQAAVNGWFASHRPQVVFLAAAKVGGIAANDA
LRGEFIYDNIMIAANVIHAAHRVGVEKLMCLGSSCIFPKFAPQPLREEALLTGLLEPTNEPYAIAKIAAIKLVEAYRSQF
GSDFINVMPTNVYGPGDNFHPEYSHVVAALIRRFHEAKMADATNVLVWGSGTPRREFLYVDDLADACVHLMRNYSDPQFI
NVGTGTDLTIAELAKVIASVVGYAGEISFDSSRPDGTPRKLLDVSRLTGLGWRARTSLQDGIRLAYEAFIDRPASRA

Sequences:

>Translated_317_residues
MANLPFELKGKRIYVAGHAGMVGGALMRRLEQEDVETLVAARAELDLRDQAAVNGWFASHRPQVVFLAAAKVGGIAANDA
LRGEFIYDNIMIAANVIHAAHRVGVEKLMCLGSSCIFPKFAPQPLREEALLTGLLEPTNEPYAIAKIAAIKLVEAYRSQF
GSDFINVMPTNVYGPGDNFHPEYSHVVAALIRRFHEAKMADATNVLVWGSGTPRREFLYVDDLADACVHLMRNYSDPQFI
NVGTGTDLTIAELAKVIASVVGYAGEISFDSSRPDGTPRKLLDVSRLTGLGWRARTSLQDGIRLAYEAFIDRPASRA
>Mature_316_residues
ANLPFELKGKRIYVAGHAGMVGGALMRRLEQEDVETLVAARAELDLRDQAAVNGWFASHRPQVVFLAAAKVGGIAANDAL
RGEFIYDNIMIAANVIHAAHRVGVEKLMCLGSSCIFPKFAPQPLREEALLTGLLEPTNEPYAIAKIAAIKLVEAYRSQFG
SDFINVMPTNVYGPGDNFHPEYSHVVAALIRRFHEAKMADATNVLVWGSGTPRREFLYVDDLADACVHLMRNYSDPQFIN
VGTGTDLTIAELAKVIASVVGYAGEISFDSSRPDGTPRKLLDVSRLTGLGWRARTSLQDGIRLAYEAFIDRPASRA

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=284, Percent_Identity=34.1549295774648, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=23.0769230769231, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI42516563, Length=318, Percent_Identity=25.4716981132075, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=57.0063694267516, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17554428, Length=308, Percent_Identity=29.8701298701299, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922778, Length=315, Percent_Identity=31.7460317460317, Blast_Score=165, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34630; Mature: 34499

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLPFELKGKRIYVAGHAGMVGGALMRRLEQEDVETLVAARAELDLRDQAAVNGWFASH
CCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHCC
RPQVVFLAAAKVGGIAANDALRGEFIYDNIMIAANVIHAAHRVGVEKLMCLGSSCIFPKF
CCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
APQPLREEALLTGLLEPTNEPYAIAKIAAIKLVEAYRSQFGSDFINVMPTNVYGPGDNFH
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCC
PEYSHVVAALIRRFHEAKMADATNVLVWGSGTPRREFLYVDDLADACVHLMRNYSDPQFI
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEE
NVGTGTDLTIAELAKVIASVVGYAGEISFDSSRPDGTPRKLLDVSRLTGLGWRARTSLQD
ECCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCHHH
GIRLAYEAFIDRPASRA
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANLPFELKGKRIYVAGHAGMVGGALMRRLEQEDVETLVAARAELDLRDQAAVNGWFASH
CCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHCC
RPQVVFLAAAKVGGIAANDALRGEFIYDNIMIAANVIHAAHRVGVEKLMCLGSSCIFPKF
CCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
APQPLREEALLTGLLEPTNEPYAIAKIAAIKLVEAYRSQFGSDFINVMPTNVYGPGDNFH
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCC
PEYSHVVAALIRRFHEAKMADATNVLVWGSGTPRREFLYVDDLADACVHLMRNYSDPQFI
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEE
NVGTGTDLTIAELAKVIASVVGYAGEISFDSSRPDGTPRKLLDVSRLTGLGWRARTSLQD
ECCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCHHH
GIRLAYEAFIDRPASRA
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]