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The map label for this gene is yafC [H]

Identifier: 86748636

GI number: 86748636

Start: 1703890

End: 1704813

Strand: Reverse

Name: yafC [H]

Synonym: RPB_1511

Alternate gene names: 86748636

Gene position: 1704813-1703890 (Counterclockwise)

Preceding gene: 86748637

Following gene: 86748629

Centisome position: 31.98

GC content: 64.61

Gene sequence:

>924_bases
ATGGCTCGACACCGCGACGGGTTCACCGATATGGATTGGGATAAGCTCAAGGTCTTCCACGCCGCCGCCGAAGCCGGCAG
CTTCACCCATGCGGGTGAGCAGCTCGGGCTGTCGCAATCGGCGGTGTCGCGGCAGGTGAGCGCGCTGGAGCAGGAGCTTT
CGGTCTCGCTGTTCCACCGCCACGCCCGCGGCCTGATCCTGACCGAACAGGGCGACCTGCTGTTCCGCACCGCGCACGAC
GTGTTCATGCAGCTCCAGGCCGCGCGCGCCAAGCTGACAGACAGCCGCGAACGGCCGAGCGGAGATCTCAAGATCACCAC
CACCCCCGGCCTGGGCATTCACTGGTTGGTGCCGCGGCTCGGCGAATTCACCGCGCTGTATCCCGAGATCCGGATCTCGC
TGATCGTCACCGACGAAGAACTCGACCTGTCGATGCGCGAGGCCGACGTCGCGATCCGGACCCGCAAGCCGACCCAGCCC
GACCTGATCCAGCGCAAGCTGTTCTCGATCGGCTTCCACGCTTATTGCTCGCCGGAATACATCAAGCATTTCGGCACGCC
GCGGACGCTCGAGGAACTCGACAATCACCGCCTGATCATGTTGAGCGACAGCCAGGTGCCGCCGCATCTGCAGAACCGGA
GCTGGCTGGTCGACGCCGGCCGCAACGGCTCCGGCCCCCGCGAGCCGTATTTCAAGGTCAACAACATTCTCGGCCTGGTG
CGCGCCTGCCAGCAGGGCCTCGGCATCGCCGCGCTGCCGGACTACCTGGTCGAAGATCCCAACCGGCTGGTGCAATTGTT
CGGCGAGGAAGATTCAATCCAGCTCGACACCTATTTCGTCTATCCGGAAGAGTTGAAGACGGTGGCGCGCGTGCAGGTGT
TCCGCGATTTCGTGGTCAACAAGGCGCAGCGCTGGCCGAGCTGA

Upstream 100 bases:

>100_bases
GCCAGGCCGTCACCGCCGCGGGCCTCGGCTGCATGGCCGCGCTGGAAGCCGAACGCTTCCTCGCCCTGCGCGCCAGCGAA
CGCCAGGCGGCGGAGTAGAC

Downstream 100 bases:

>100_bases
AGGCCCCGGTCCCCGCATGACTGACATGCGGGCGCCCTGCTTGCCGCGACTGGCGTTCGCAGATCAAATGGCTCCACGCT
GAGGGTTCGCACTACGCATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MARHRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELSVSLFHRHARGLILTEQGDLLFRTAHD
VFMQLQAARAKLTDSRERPSGDLKITTTPGLGIHWLVPRLGEFTALYPEIRISLIVTDEELDLSMREADVAIRTRKPTQP
DLIQRKLFSIGFHAYCSPEYIKHFGTPRTLEELDNHRLIMLSDSQVPPHLQNRSWLVDAGRNGSGPREPYFKVNNILGLV
RACQQGLGIAALPDYLVEDPNRLVQLFGEEDSIQLDTYFVYPEELKTVARVQVFRDFVVNKAQRWPS

Sequences:

>Translated_307_residues
MARHRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELSVSLFHRHARGLILTEQGDLLFRTAHD
VFMQLQAARAKLTDSRERPSGDLKITTTPGLGIHWLVPRLGEFTALYPEIRISLIVTDEELDLSMREADVAIRTRKPTQP
DLIQRKLFSIGFHAYCSPEYIKHFGTPRTLEELDNHRLIMLSDSQVPPHLQNRSWLVDAGRNGSGPREPYFKVNNILGLV
RACQQGLGIAALPDYLVEDPNRLVQLFGEEDSIQLDTYFVYPEELKTVARVQVFRDFVVNKAQRWPS
>Mature_306_residues
ARHRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELSVSLFHRHARGLILTEQGDLLFRTAHDV
FMQLQAARAKLTDSRERPSGDLKITTTPGLGIHWLVPRLGEFTALYPEIRISLIVTDEELDLSMREADVAIRTRKPTQPD
LIQRKLFSIGFHAYCSPEYIKHFGTPRTLEELDNHRLIMLSDSQVPPHLQNRSWLVDAGRNGSGPREPYFKVNNILGLVR
ACQQGLGIAALPDYLVEDPNRLVQLFGEEDSIQLDTYFVYPEELKTVARVQVFRDFVVNKAQRWPS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=31.438127090301, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=30.7432432432432, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=28, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787128, Length=192, Percent_Identity=31.25, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=28.8135593220339, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789440, Length=189, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=27.681660899654, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787589, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=25.7731958762887, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788706, Length=220, Percent_Identity=29.0909090909091, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=190, Percent_Identity=29.4736842105263, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=28.8537549407115, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=24.4979919678715, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788626, Length=191, Percent_Identity=29.3193717277487, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34830; Mature: 34699

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELSVSLFHR
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HARGLILTEQGDLLFRTAHDVFMQLQAARAKLTDSRERPSGDLKITTTPGLGIHWLVPRL
HHCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEHHHHH
GEFTALYPEIRISLIVTDEELDLSMREADVAIRTRKPTQPDLIQRKLFSIGFHAYCSPEY
CCHHEECCEEEEEEEEECCHHCCHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHH
IKHFGTPRTLEELDNHRLIMLSDSQVPPHLQNRSWLVDAGRNGSGPREPYFKVNNILGLV
HHHCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
RACQQGLGIAALPDYLVEDPNRLVQLFGEEDSIQLDTYFVYPEELKTVARVQVFRDFVVN
HHHHHCCCEECCCHHHHHCHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHH
KAQRWPS
HHHCCCC
>Mature Secondary Structure 
ARHRDGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQVSALEQELSVSLFHR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HARGLILTEQGDLLFRTAHDVFMQLQAARAKLTDSRERPSGDLKITTTPGLGIHWLVPRL
HHCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEHHHHH
GEFTALYPEIRISLIVTDEELDLSMREADVAIRTRKPTQPDLIQRKLFSIGFHAYCSPEY
CCHHEECCEEEEEEEEECCHHCCHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHH
IKHFGTPRTLEELDNHRLIMLSDSQVPPHLQNRSWLVDAGRNGSGPREPYFKVNNILGLV
HHHCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
RACQQGLGIAALPDYLVEDPNRLVQLFGEEDSIQLDTYFVYPEELKTVARVQVFRDFVVN
HHHHHCCCEECCCHHHHHCHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHH
KAQRWPS
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]