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The map label for this gene is dadB [H]

Identifier: 86748627

GI number: 86748627

Start: 1696860

End: 1698026

Strand: Direct

Name: dadB [H]

Synonym: RPB_1502

Alternate gene names: 86748627

Gene position: 1696860-1698026 (Clockwise)

Preceding gene: 86748625

Following gene: 86748628

Centisome position: 31.83

GC content: 66.84

Gene sequence:

>1167_bases
ATGAGGCTGGGGCATCGTCATGCCGGGGCGACGCTGACGATCGATCTGGCGGCCATCCGGTGGAATTATCGCCGTCTGGC
CGAGATGGTAGGACCGAAGGTCCGCTGCGGCGGGGTCGTCAAGGCGGATGCCTACGGGTTGGGTGCGCGCCAGGTCGCTC
CGGCGCTCTATGGCGCCGGATGCCGGGATTTCTATGTCGCCCATCTCGATGAGGCGCTCGATCTGCGACCGCATCTGCCG
CCCGACGCATCGATCGTGGTCCTGAACGGGCTGCCGCGCGGCGGCGAAGACGATTGCGCCGATGCAGGCATCGTGCCCGT
CCTCAATTCGCTCGCGCAACTCGATGACTGGTCGGCCTGCGCGCGCAAGGCGGGCCGCGTGCTGGGGGCGATCGTACAGA
TCGACAGCGGCATGTCGCGGCTCGGGCTCTCGCCACGAGAACTTGACGAGCTTTCGCACGCGCGCCTGCGCGGCATCGAT
ATTCGCCTGGTGATGAGCCATCTGGCCTGCGCCGATGTCGCCGATCACGCGGCGAATGCGTATCAGCGGGCGCGGTTCTG
TGCGCTGGCCGATCGCTTCGGAGGGATTCCGAAGTCGCTGGCGAATTCGTCCGGAATCTTTCTCGGCCCCGACTTTCACT
ACGACGCCGCCCGGCCGGGTGCGGCGCTCTACGGCATCAATCCCACGTCGTCCCGGCCCAACCCGATGCGGCCGGTGGTT
CGGCTCGCGGCCGCGATCGTTCAGACGCGCGAGATCGAAGCCGGCGATCACGTCGGCTACGGCTGGGATTTCCGCGTCGA
ACGGCCGTCGCGGTTGGCGACGCTGTCGATCGGTTATGCCGACGGCCTGCATCGCGCGCTGGGGCGAGCCGGCGCGGTGT
ACTTCGAGGGCCGCGCCCTTGCGATCGCCGGACGGGTGTCGATGGACAGCATCACCGTCGATCTCGGTGATCTGCCGGCC
GAGCGCCTCGGGCGTGGCGCAGAGATCGAACTGATCGGTCCGCATCAGTCGGTCGACGAACTCGCCTCCGCGAGCGGCAC
CATCGGCTACGAAATCCTGACCAGCCTCGGCGATCGCTTTCGACGGATTTATCGCGATGCCGCCGACCGGCCGCAGGACA
GTGACCTCCAAAAAAGAGACGATGCCATGAAAGCGCTGATGCCATGA

Upstream 100 bases:

>100_bases
GAATGGACGGTTACCATAAAAATTCGCCAACAACCACCGCGCTCTTCCTGACATCCTGTTGCAGGCGACACTGGCAGAGG
ATGGGGACGTGAGCGTCGAG

Downstream 100 bases:

>100_bases
AAGTTCTGGTGATGGGAGCCGGCGTGATCGGCGTCACGACGGCTTATTATCTGGCCAAGGCCGGCTTCGAGGTCACCGTG
ATCGACCGTCAGCCCGGCCC

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRLGHRHAGATLTIDLAAIRWNYRRLAEMVGPKVRCGGVVKADAYGLGARQVAPALYGAGCRDFYVAHLDEALDLRPHLP
PDASIVVLNGLPRGGEDDCADAGIVPVLNSLAQLDDWSACARKAGRVLGAIVQIDSGMSRLGLSPRELDELSHARLRGID
IRLVMSHLACADVADHAANAYQRARFCALADRFGGIPKSLANSSGIFLGPDFHYDAARPGAALYGINPTSSRPNPMRPVV
RLAAAIVQTREIEAGDHVGYGWDFRVERPSRLATLSIGYADGLHRALGRAGAVYFEGRALAIAGRVSMDSITVDLGDLPA
ERLGRGAEIELIGPHQSVDELASASGTIGYEILTSLGDRFRRIYRDAADRPQDSDLQKRDDAMKALMP

Sequences:

>Translated_388_residues
MRLGHRHAGATLTIDLAAIRWNYRRLAEMVGPKVRCGGVVKADAYGLGARQVAPALYGAGCRDFYVAHLDEALDLRPHLP
PDASIVVLNGLPRGGEDDCADAGIVPVLNSLAQLDDWSACARKAGRVLGAIVQIDSGMSRLGLSPRELDELSHARLRGID
IRLVMSHLACADVADHAANAYQRARFCALADRFGGIPKSLANSSGIFLGPDFHYDAARPGAALYGINPTSSRPNPMRPVV
RLAAAIVQTREIEAGDHVGYGWDFRVERPSRLATLSIGYADGLHRALGRAGAVYFEGRALAIAGRVSMDSITVDLGDLPA
ERLGRGAEIELIGPHQSVDELASASGTIGYEILTSLGDRFRRIYRDAADRPQDSDLQKRDDAMKALMP
>Mature_388_residues
MRLGHRHAGATLTIDLAAIRWNYRRLAEMVGPKVRCGGVVKADAYGLGARQVAPALYGAGCRDFYVAHLDEALDLRPHLP
PDASIVVLNGLPRGGEDDCADAGIVPVLNSLAQLDDWSACARKAGRVLGAIVQIDSGMSRLGLSPRELDELSHARLRGID
IRLVMSHLACADVADHAANAYQRARFCALADRFGGIPKSLANSSGIFLGPDFHYDAARPGAALYGINPTSSRPNPMRPVV
RLAAAIVQTREIEAGDHVGYGWDFRVERPSRLATLSIGYADGLHRALGRAGAVYFEGRALAIAGRVSMDSITVDLGDLPA
ERLGRGAEIELIGPHQSVDELASASGTIGYEILTSLGDRFRRIYRDAADRPQDSDLQKRDDAMKALMP

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1790487, Length=368, Percent_Identity=32.8804347826087, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1787439, Length=357, Percent_Identity=31.0924369747899, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 41674; Mature: 41674

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGHRHAGATLTIDLAAIRWNYRRLAEMVGPKVRCGGVVKADAYGLGARQVAPALYGAG
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCHHHHHHHHHCCC
CRDFYVAHLDEALDLRPHLPPDASIVVLNGLPRGGEDDCADAGIVPVLNSLAQLDDWSAC
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
ARKAGRVLGAIVQIDSGMSRLGLSPRELDELSHARLRGIDIRLVMSHLACADVADHAANA
HHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YQRARFCALADRFGGIPKSLANSSGIFLGPDFHYDAARPGAALYGINPTSSRPNPMRPVV
HHHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCHHHHH
RLAAAIVQTREIEAGDHVGYGWDFRVERPSRLATLSIGYADGLHRALGRAGAVYFEGRAL
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHCCCEEEECCEEE
AIAGRVSMDSITVDLGDLPAERLGRGAEIELIGPHQSVDELASASGTIGYEILTSLGDRF
EEECCEEECEEEEECCCCCHHHCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
RRIYRDAADRPQDSDLQKRDDAMKALMP
HHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLGHRHAGATLTIDLAAIRWNYRRLAEMVGPKVRCGGVVKADAYGLGARQVAPALYGAG
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCHHHHHHHHHCCC
CRDFYVAHLDEALDLRPHLPPDASIVVLNGLPRGGEDDCADAGIVPVLNSLAQLDDWSAC
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
ARKAGRVLGAIVQIDSGMSRLGLSPRELDELSHARLRGIDIRLVMSHLACADVADHAANA
HHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YQRARFCALADRFGGIPKSLANSSGIFLGPDFHYDAARPGAALYGINPTSSRPNPMRPVV
HHHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCHHHHH
RLAAAIVQTREIEAGDHVGYGWDFRVERPSRLATLSIGYADGLHRALGRAGAVYFEGRAL
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHCCCEEEECCEEE
AIAGRVSMDSITVDLGDLPAERLGRGAEIELIGPHQSVDELASASGTIGYEILTSLGDRF
EEECCEEECEEEEECCCCCHHHCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
RRIYRDAADRPQDSDLQKRDDAMKALMP
HHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]