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The map label for this gene is yfeR [H]

Identifier: 86748625

GI number: 86748625

Start: 1695143

End: 1696126

Strand: Direct

Name: yfeR [H]

Synonym: RPB_1500

Alternate gene names: 86748625

Gene position: 1695143-1696126 (Clockwise)

Preceding gene: 86748618

Following gene: 86748627

Centisome position: 31.79

GC content: 61.08

Gene sequence:

>984_bases
ATGCCTAAATATATTACAAATGAATTAGTAGCATCGTTGATTTCGAGAATCGAATTAATGGCTGAACCGACCCTGAAACA
GATTCGTATGTTTCTCGCTGTGGCCAGGCACCGCAGTTTTCGTGTCGCGGGCGACCGGGTTTCGGCGTCCCAGTCGAGTA
TTTCCATTCAGATCAAGCAGTTGGAAGATCGTGTCGGGCTTGCTTTGTTCGACCGCACGACCCGGGTGGTGAAACTGACC
CCGGCGGGCTCGGAACTGCTCGCGGATTTCGAGCGTCTCGCGGCCATGGCCGACGAGGTCCGCGTCAAATGTGCGCAATT
GGCCGCCGGGCGAACCGGTCAACTCAGGATCGGGGTGCTTCCGTCGATTGCCGAGCATTTGCTTCCCGAGGTGATCGCCG
AGTTCAGCCGCGAGCTTCCCGGCGTTCACTTTGAGATCTTTGAAGCCGTCGAGCAGGAATTGATCGCCGCCGTCAAAGTC
GGGCGATGTGATCTCGGCCTGACCAGCGCGCGCATGCTCGAGCGTGGCATGACGTTCGAGGGATTGTTCAGCGACGACCT
GTTCGCCGTCATGCTGTCGTCGCACCCCCTGGCCTCGCTGGACGAAATTCCAATCGCGGCTCTCGCCGAGCGGCCGCTCA
TCTTGACGAAATGGGGGACCAGTCTGCGTCTGGCGGCGAGCCAGGCCTTCGAGGACGAGGGCATCGCCATCGTGCCCGCC
TATGAAGTGACGAACATGGCGACGGCCCTTGGATTTGCCGCGAAGGGTCTCGGCGTCGCGCTGCTGCCGGCCAGCGCAGT
CAGGAACATGCAGAACGTCGCGACCGTCGCGAGGCCGCTGCGGGGGCAGGCTGGTTCGCGCATGATGGGCATTCTTCAGC
TCGAAGGCGCCGTCAGCTATCCTCTCCAGCAAAGGTTCATCGCGCTGCTGCGCAGCGTCGTTGCGACAGCCGCGTCGCGA
CAGGTGCTTGCGCCGGAATCATGA

Upstream 100 bases:

>100_bases
CTGCTCATGAACTTCCCCGTCACCCCTTGCGATGTGTCGCAAGCTCGTTGTCGTCAGCCCTTGCGGCCAACCTCCAAATA
CCTGAGCCCTATTGTTGGGC

Downstream 100 bases:

>100_bases
ACTCAAGGCCCGCGGCGGTCAGATCGCAGCAGTGCGGAGCGGCTGTCCCGGGGGGGGGGGCGGCTTTATCGCATGGGCGC
CGGGATGGGCTAACCGCCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPKYITNELVASLISRIELMAEPTLKQIRMFLAVARHRSFRVAGDRVSASQSSISIQIKQLEDRVGLALFDRTTRVVKLT
PAGSELLADFERLAAMADEVRVKCAQLAAGRTGQLRIGVLPSIAEHLLPEVIAEFSRELPGVHFEIFEAVEQELIAAVKV
GRCDLGLTSARMLERGMTFEGLFSDDLFAVMLSSHPLASLDEIPIAALAERPLILTKWGTSLRLAASQAFEDEGIAIVPA
YEVTNMATALGFAAKGLGVALLPASAVRNMQNVATVARPLRGQAGSRMMGILQLEGAVSYPLQQRFIALLRSVVATAASR
QVLAPES

Sequences:

>Translated_327_residues
MPKYITNELVASLISRIELMAEPTLKQIRMFLAVARHRSFRVAGDRVSASQSSISIQIKQLEDRVGLALFDRTTRVVKLT
PAGSELLADFERLAAMADEVRVKCAQLAAGRTGQLRIGVLPSIAEHLLPEVIAEFSRELPGVHFEIFEAVEQELIAAVKV
GRCDLGLTSARMLERGMTFEGLFSDDLFAVMLSSHPLASLDEIPIAALAERPLILTKWGTSLRLAASQAFEDEGIAIVPA
YEVTNMATALGFAAKGLGVALLPASAVRNMQNVATVARPLRGQAGSRMMGILQLEGAVSYPLQQRFIALLRSVVATAASR
QVLAPES
>Mature_326_residues
PKYITNELVASLISRIELMAEPTLKQIRMFLAVARHRSFRVAGDRVSASQSSISIQIKQLEDRVGLALFDRTTRVVKLTP
AGSELLADFERLAAMADEVRVKCAQLAAGRTGQLRIGVLPSIAEHLLPEVIAEFSRELPGVHFEIFEAVEQELIAAVKVG
RCDLGLTSARMLERGMTFEGLFSDDLFAVMLSSHPLASLDEIPIAALAERPLILTKWGTSLRLAASQAFEDEGIAIVPAY
EVTNMATALGFAAKGLGVALLPASAVRNMQNVATVARPLRGQAGSRMMGILQLEGAVSYPLQQRFIALLRSVVATAASRQ
VLAPES

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=30.7377049180328, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693105, Length=286, Percent_Identity=26.5734265734266, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=32.3886639676113, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=28.7162162162162, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=27.9461279461279, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=29.6577946768061, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787128, Length=150, Percent_Identity=38, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081904, Length=284, Percent_Identity=25.7042253521127, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=251, Percent_Identity=26.2948207171315, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788296, Length=216, Percent_Identity=27.3148148148148, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787949, Length=247, Percent_Identity=27.1255060728745, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=30.8988764044944, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789173, Length=191, Percent_Identity=31.413612565445, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=25.1724137931034, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786713, Length=277, Percent_Identity=27.4368231046931, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788481, Length=267, Percent_Identity=26.2172284644195, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=25.3968253968254, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=119, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789204, Length=179, Percent_Identity=29.0502793296089, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI48994962, Length=267, Percent_Identity=25.0936329588015, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790262, Length=286, Percent_Identity=24.1258741258741, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35332; Mature: 35201

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYITNELVASLISRIELMAEPTLKQIRMFLAVARHRSFRVAGDRVSASQSSISIQIKQ
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHEEEHEE
LEDRVGLALFDRTTRVVKLTPAGSELLADFERLAAMADEVRVKCAQLAAGRTGQLRIGVL
HHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
PSIAEHLLPEVIAEFSRELPGVHFEIFEAVEQELIAAVKVGRCDLGLTSARMLERGMTFE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
GLFSDDLFAVMLSSHPLASLDEIPIAALAERPLILTKWGTSLRLAASQAFEDEGIAIVPA
CCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEECCCCEEHHHHHHHCCCCEEEEEC
YEVTNMATALGFAAKGLGVALLPASAVRNMQNVATVARPLRGQAGSRMMGILQLEGAVSY
HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCCC
PLQQRFIALLRSVVATAASRQVLAPES
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PKYITNELVASLISRIELMAEPTLKQIRMFLAVARHRSFRVAGDRVSASQSSISIQIKQ
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHEEEHEE
LEDRVGLALFDRTTRVVKLTPAGSELLADFERLAAMADEVRVKCAQLAAGRTGQLRIGVL
HHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
PSIAEHLLPEVIAEFSRELPGVHFEIFEAVEQELIAAVKVGRCDLGLTSARMLERGMTFE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
GLFSDDLFAVMLSSHPLASLDEIPIAALAERPLILTKWGTSLRLAASQAFEDEGIAIVPA
CCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEECCCCEEHHHHHHHCCCCEEEEEC
YEVTNMATALGFAAKGLGVALLPASAVRNMQNVATVARPLRGQAGSRMMGILQLEGAVSY
HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCCC
PLQQRFIALLRSVVATAASRQVLAPES
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]