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The map label for this gene is ntcA [H]

Identifier: 86748600

GI number: 86748600

Start: 1664807

End: 1665589

Strand: Reverse

Name: ntcA [H]

Synonym: RPB_1475

Alternate gene names: 86748600

Gene position: 1665589-1664807 (Counterclockwise)

Preceding gene: 86748601

Following gene: 86748599

Centisome position: 31.24

GC content: 64.62

Gene sequence:

>783_bases
ATGCAGGTTCTCGCCTCAGTCAAATCCGCAACGAATCCGGTGGCGTCCCGCCCGGCCGTCGCATCGCAGGCGGGACACTC
CCTGCTGCTGACGGAAAGTGCCCGTTCGCTGGGCGGTCCGCCGTCTCTGCTGGCAGCGCTGACCGATCGCGAGCGTGAGA
TCGTGCTGCGGCACGGCCGGCGCAAGGTGCTGTATCGCGGCAAGACGCTGTTCAATCAGGGCGCCAAACACGACGGCATC
TATCTGATCGAGACCGGACGAATCCGCGTGTTCTACACCGCCCCATCCGGCCGCGAGATCACACTCGCTTATTGGCATCC
GGGCAATTTCGTCGGCGGACCGGAAGTCTTCGGCAGCGGCGTGCATCAGTGGTCCGGCGTCGCGACCAGCAACTCCAACA
TCGTGCAATTGCCCGGCAAGGATCTGCGCGCGCTGGTCGTCGAGGTTCCGGGTCTGGCGATCGGGCTGATCGAGGGGCTG
ACCTTCAAGGGCAAATGCTACTCGGCGCTGGCGCAGATGCTGGGCACCCGCTCGATCACCGAAAGGCTGGCGCATCTGCT
GCTGCATCTCGCCCAGCTCTACGGCGTCGACGATGCCGACGGCGTCCTGATCGGTGCGGCTTTCACCCATGCCGATCTCG
CCCACATGGTCGGCGCCACACGGCAATGGGTGACGATCAGCCTGAAACGGATGCAGGAGAAGGGCATCGTCGCCTCGCGG
CGCTCACAGATCGTGATTCTGAGACCTGATCTGCTGCAGGAGATGCGCGGGCTCGGCGACTGA

Upstream 100 bases:

>100_bases
ACGCGTGCGACCGGGAAGCGATCGACCACGCACGGCATAGTTGCGAGCGATTGGCACGAGGTTTGCTGCGTGTTTAGAAC
ACGTCAAATCCGGAGTGCGC

Downstream 100 bases:

>100_bases
CGTGCCTGCCTATGGTTGCGGCTACCGACGCTCAGACTGCTCAAGTGCTGAGCAGCCGTTCTATCGCGGCAACTAATCGA
CCACTCGACTGCCACGACGT

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQVLASVKSATNPVASRPAVASQAGHSLLLTESARSLGGPPSLLAALTDREREIVLRHGRRKVLYRGKTLFNQGAKHDGI
YLIETGRIRVFYTAPSGREITLAYWHPGNFVGGPEVFGSGVHQWSGVATSNSNIVQLPGKDLRALVVEVPGLAIGLIEGL
TFKGKCYSALAQMLGTRSITERLAHLLLHLAQLYGVDDADGVLIGAAFTHADLAHMVGATRQWVTISLKRMQEKGIVASR
RSQIVILRPDLLQEMRGLGD

Sequences:

>Translated_260_residues
MQVLASVKSATNPVASRPAVASQAGHSLLLTESARSLGGPPSLLAALTDREREIVLRHGRRKVLYRGKTLFNQGAKHDGI
YLIETGRIRVFYTAPSGREITLAYWHPGNFVGGPEVFGSGVHQWSGVATSNSNIVQLPGKDLRALVVEVPGLAIGLIEGL
TFKGKCYSALAQMLGTRSITERLAHLLLHLAQLYGVDDADGVLIGAAFTHADLAHMVGATRQWVTISLKRMQEKGIVASR
RSQIVILRPDLLQEMRGLGD
>Mature_260_residues
MQVLASVKSATNPVASRPAVASQAGHSLLLTESARSLGGPPSLLAALTDREREIVLRHGRRKVLYRGKTLFNQGAKHDGI
YLIETGRIRVFYTAPSGREITLAYWHPGNFVGGPEVFGSGVHQWSGVATSNSNIVQLPGKDLRALVVEVPGLAIGLIEGL
TFKGKCYSALAQMLGTRSITERLAHLLLHLAQLYGVDDADGVLIGAAFTHADLAHMVGATRQWVTISLKRMQEKGIVASR
RSQIVILRPDLLQEMRGLGD

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=198, Percent_Identity=24.2424242424242, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789756, Length=201, Percent_Identity=25.8706467661692, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28059; Mature: 28059

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVLASVKSATNPVASRPAVASQAGHSLLLTESARSLGGPPSLLAALTDREREIVLRHGR
CCHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCHHHCCCCHHHHHHHHCCHHHHHHHCCC
RKVLYRGKTLFNQGAKHDGIYLIETGRIRVFYTAPSGREITLAYWHPGNFVGGPEVFGSG
CEEEECCHHHHHCCCCCCCEEEEEECCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCC
VHQWSGVATSNSNIVQLPGKDLRALVVEVPGLAIGLIEGLTFKGKCYSALAQMLGTRSIT
HHHHCCEEECCCCEEEECCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHH
ERLAHLLLHLAQLYGVDDADGVLIGAAFTHADLAHMVGATRQWVTISLKRMQEKGIVASR
HHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCHHEEHHHHHHHHCCCEECC
RSQIVILRPDLLQEMRGLGD
CCCEEEECHHHHHHHHCCCC
>Mature Secondary Structure
MQVLASVKSATNPVASRPAVASQAGHSLLLTESARSLGGPPSLLAALTDREREIVLRHGR
CCHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCHHHCCCCHHHHHHHHCCHHHHHHHCCC
RKVLYRGKTLFNQGAKHDGIYLIETGRIRVFYTAPSGREITLAYWHPGNFVGGPEVFGSG
CEEEECCHHHHHCCCCCCCEEEEEECCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCC
VHQWSGVATSNSNIVQLPGKDLRALVVEVPGLAIGLIEGLTFKGKCYSALAQMLGTRSIT
HHHHCCEEECCCCEEEECCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHCCHHHH
ERLAHLLLHLAQLYGVDDADGVLIGAAFTHADLAHMVGATRQWVTISLKRMQEKGIVASR
HHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCHHEEHHHHHHHHCCCEECC
RSQIVILRPDLLQEMRGLGD
CCCEEEECHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630321 [H]